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| 1 | Responses of wheat seedlings to cadmium,mercury and trichlorobenzene stresses显示文摘The molecular response of wheat(Triticum aestivum L.,cv.Yangmai 13) seedlings to heavy metal(Cd,Hg) and 1,2,4-trichlorobenzene(TCB) stresses were examined by two-dimensional gel electrophoresis,image analysis,and peptide mass fingerprinting.The results showed inhibitions of root and shoot growth by Cd,Hg,and TCB.These stresses led to water deficit and lipid phosphorylation in the seedling which also promoted protein phophorylation in the leaves.Hg stress inhibited protein synthesis while Cd and TCB stresses induced or up-regulated more proteins in the leaves.Most of these induced proteins played important roles in the biochemical reactions involved in tolerance of wheat to Cd and TCB stresses.The primary functions of Cd-and TCB-induced proteins included methionine metabolism,Rubisco modification,protein phosphorylation regulation,protein configuration protection,H+ transmembrane transportation and also the synthesis of ethylene,defense substances and cell wall compounds. | GE Cailin DING Yan WANG Zegang WAN Dingzhen WANG Yulong SHANG Qi LUO Shishi | 2009 | Journal of Environmental Sciences2009，21，6： | 12 |
| 2 | A proteomic analysis of rice seedlings responding to 1,2,4-trichlorobenzene stress显示文摘回答 to1,2,4-trichlorobenzene (TCB ) 的根和叶子强调的米饭(Oryza sativa L.) 的 proteomic 分析被二维的胶化电气泳动执行,质量度谱(MS ) ,并且蛋白质数据库分析。结果证明 5 mg/L TCB 应力在米饭根和叶子在全球 proteome 上有重要效果。范畴的分析和 TCB 的功能强调可诱导的蛋白质证明回答的不同类型在米饭根和叶子被生产,当稻秧暴露于 5mg/L TCB 应力时。大多数回答为保卫 TCB 应力的损坏的米饭是必要的。这些回答包括有毒的物质,致病相关的蛋白质的表示,细胞壁物质的合成和第二等的混合物的解毒,蛋白质的规定和氨基酸新陈代谢,蛋氨酸的激活抢救小径,并且也包括渗透的规定和植物激素新陈代谢。比较 TCB 压力在二栽培变种,β-葡萄糖苷酶和致病相关的蛋白质之间的可诱导的蛋白质家庭 10 蛋白质被 TCB 应力特别地在米饭栽培变种( Oryza sativa L.)的根导致 Aizaizhan ,并且谷胱甘肽 S-transferase 和 aci-reductone dioxygenase 4 在米饭栽培变种 Shanyou 63 的根被导致。这可以是为比 Aizaizhan 有更高的公差到 TCB 应力的 Shanyou 63 的重要机制之一。 | GE Cailin WAN Dingzhen WANG Zegang DING Yan WANG Yulong SHANG Qi MA Fai LUO Shishi | 2008 | Journal of Environmental Sciences2008，20，3： | 7 |
| 3 | Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes显示文摘The diploid species Brassica rapa(genome AA)and B.oleracea(genome CC)were compared by full-scale proteome analyses of seedling.A total of 28.2% of the proteins was common to both species,indicating the existence of a basal or ubiquitous proteome.However,a number of discriminating proteins(32.0%)and specific proteins(39.8%)of the Brassica A and C genomes,respectively,were identified,which could represent potentially species-specific functions.Based on these A or C genome-specific proteins,a number of PCR-based markers to distinguish B.rapa and B.oleracea species were also developed. | Fang Kong Cailin Ge Xiaoping Fang Rod J. Snowdon Youping Wang | 2010 | Journal of Genetics and Genomics2010，37，5： | 3 |
| 4 | Expression of sulfur uptake assimilation-related genes in response to cadmium,bensulfuron-methyl and their co-contamination in rice roots显示文摘The responses of sulfur(S) uptake assimilation-related genes' expression in roots of two rice cultivars to cadmium(Cd), bensulfuron-methyl(BSM) and their co-contamination(Cd+BSM) were investigated by gene-chip microarray analysis and quantitative real-time PCR(QRT-PCR) technology. Treatments of Cd and Cd+BSM induced expression of sulfate transporter and permease genes, and promoted sulfate uptake in rice roots. Cd+BSM could alleviate Cd toxicity to cv. Fengmeizhan seedlings, probably due to Cd+BSM promoting greater S absorption by seedlings. Cd and Cd+BSM induced expression of sulfate assimilation-related genes, and thus activated the sulfur assimilation pathway. Cd and Cd+BSM induced expression of phytochelatin synthase and metallothionein genes, and induced expression of glutathione S-transferases(GSTs), glutathione synthase(GS) and antioxidation enzyme genes, which detoxified Cd2+. It is suggested that(to cope with the toxicity of Cd, BSM and their co-contamination) the S uptake and assimilation pathway was activated in rice roots by increased expression of related genes, thus enhancing the supply of organic S for synthesis of Cd or BSM resistance-related substances. | Jian Zhou Zegang Wang Zhiwei Huang Chao Lu Zhuo Han Jianfeng Zhang Huimin Jiang Cailin Ge Juncheng Yang | 2014 | Journal of Environmental Sciences2014，26，3： | 2 |
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