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| 1 | Aberrant activation of latent transforming growth factor-β initiates the onset of temporomandibular joint osteoarthritis显示文摘There is currently no effective medical treatment for temporomandibular joint osteoarthritis(TMJ-OA) due to a limited understanding of its pathogenesis. This study was undertaken to investigate the key role of transforming growth factor-β(TGF-β)signalling in the cartilage and subchondral bone of the TMJ using a temporomandibular joint disorder(TMD) rat model, an ageing mouse model and a Camurati–Engelmann disease(CED) mouse model. In the three animal models, the subchondral bone phenotypes in the mandibular condyles were evaluated by μCT, and changes in TMJ condyles were examined by TRAP staining and immunohistochemical analysis of Osterix and p-Smad2/3. Condyle degradation was confirmed by Safranin O staining, the Mankin and OARSI scoring systems and type X collagen(Col X), p-Smad2/3 a and Osterix immunohistochemical analyses. We found apparent histological phenotypes of TMJ-OA in the TMD, ageing and CED animal models, with abnormal activation of TGF-βsignalling in the condylar cartilage and subchondral bone. Moreover, inhibition of TGF-β receptor I attenuated TMJ-OA progression in the TMD models. Therefore, aberrant activation of TGF-β signalling could be a key player in TMJ-OA development. | Liwei Zheng Caixia Pi Jun Zhang Yi Fan Chen Cui Yang Zhou Jianxun Sun Quan Yuan Xin Xu Ling Ye Xu Cao Xuedong Zhou | 2018 | Bone Research2018，6，4： | 13 |
| 2 | Osteoporosis-decreased extracellular matrix stiffness impairs connexin 43-mediated gap junction intercellular communication in osteocytes显示文摘Osteocytes are the main sensitive and responsive cells for mechanical stimuli in bone.The connexin family enables them to communicate with each other via forming functional gap junctions.However,how osteoporosis-impaired extracellular mechanical property modulates gap junction intercellular communication in osteocytes remains elusive.In this study,we established an ovariectomy(OVX)-induced osteoporosis mouse model in vivo and a polydimethylsiloxane(PDMS)-based cell culture substrate model in vitro to explore the influence of extracellular matrix(ECM)stiffness on cell-to-cell communication in osteocytes.Firstly,we established an OVX-induced osteoporosis mouse model by characterizing the changes in radiography,morphology and histochemistry of femurs.Our results showed that osteoporosis decreased the bone matrix stiffness together with the changes including the loss of osteocytes and the decrease of protein markers.Meanwhile,the dendritic process interconnection and channel-forming protein,Cx43,were reduced in osteoporosis mice.Next we mimicked ECM stiffness changes in vitro by using PDMS substrates at ratios 1:5 for normal stiffness and 1:45 for osteoporosis stiffness.Our results showed that the decreased ECM stiffness reduced the number of dendritic processes in a single cell and gap junctions between adjacent osteocytes.We further detected the decreased expression of Cx43,in the substrate with decreased stiffness.Finally,we found that gap junction-based intercellular communication was reduced in living osteocytes in the substrate with decreased stiffness.This study demonstrates the correlation between ECM mechanical property and cell-to-cell communication in osteocytes and might pave the way for further exploration of osteoporosis in terms of biomechanics. | Demao Zhang Xin Li Caixia Pi Linyi Cai Yang Liu Wei Du Wenbin Yang Jing Xie | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 3 |
| 3 | PTHrP promotes subchondral bone formation in TMJ-OA显示文摘PTH-related peptide(PTHr P) improves the bone marrow micro-environment to activate the bone-remodelling, but the coordinated regulation of PTHr P and transforming growth factor-β(TGFβ) signalling in TMJ-OA remains incompletely understood. We used disordered occlusion to establish model animals that recapitulate the ordinary clinical aetiology of TMJ-OA. Immunohistochemical and histological analyses revealed condylar fibrocartilage degeneration in model animals following disordered occlusion. TMJ-OA model animals administered intermittent PTHr P(i PTH) exhibited significantly decreased condylar cartilage degeneration. Micro-CT,histomorphometry, and Western Blot analyses disclosed that i PTH promoted subchondral bone formation in the TMJ-OA model animals. In addition, i PTH increased the number of osterix(OSX)-positive cells and osteocalcin(OCN)-positive cells in the subchondral bone marrow cavity. However, the number of osteoclasts was also increased by i PTH, indicating that subchondral bone volume increase was mainly due to the i PTH-mediated increase in the bone-formation ability of condylar subchondral bone.In vitro, PTHr P treatment increased condylar subchondral bone marrow-derived mesenchymal stem cell(SMSC) osteoblastic differentiation potential and upregulated the gene and protein expression of key regulators of osteogenesis. Furthermore, we found that PTHr P-PTH1R signalling inhibits TGFβ signalling during osteoblastic differentiation. Collectively, these data suggested that i PTH improves OA lesions by enhancing osteoblastic differentiation in subchondral bone and suppressing aberrant active TGFβsignalling. These findings indicated that PTHr P, which targets the TGFβ signalling pathway, may be an effective biological reagent to prevent and treat TMJ-OA in the clinic. | Jun Zhang Caixia Pi Chen Cui Yang Zhou Bo Liu Juan Liu Xin Xu Xuedong Zhou Liwei Zheng | 2022 | International Journal of Oral Science2022，14，4： | 1 |
| 4 | Effects of In-video Questions and Feedback on Learning Performance显示文摘The eye-tracking technology was used in this study to investigate the effects of embedded questions and feedback in instructional videos on learning performance and attention allocation and whether an expertise reversal effect existed.The experiment involved 49 learners with high-level prior knowledge and 45 ones with low-level prior knowledge from a university.Meanwhile,they learned instructional videos with no embedded feedback,embedded questions without feedback and embedded questions with feedback.Findings from the experiment showed that the instructional videos with embedded questions but without feedback not only improved the participants’attention but also enhanced their learning performance.Furthermore,there was an expertise reversal effect on the learning performance whereby instructional videos with embedded questions but without feedback improved the learning performance of learners with low-level prior knowledge,but not those with high-level prior knowledge. | XIE Yaohui YANG Jiumin PI Zhongling DAI Chenyan LIU Caixia | 2022 | Frontiers of Education in China2022，17，1： | 0 |
| 5 | Expert Consensus on Nutritional Support for Children with Congenital Heart Disease(2023 Edition)显示文摘The second edition of the expert consensus on pediatric nutrition was formed based on a global update of pedia-tric nutrition guidelines or consensus worldwide,the management of congenital heart disease,and the results of multi-center clinical nutrition research for congenital heart disease following thefirst Chinese consensus edition of 2016.The consensus was also shaped by the results of three discussion sessions and two questionnaires con-ducted by the 13-member collaboration group.This process was informed by both clinical guidelines and expert consensus.The quality of literature,both in English and Chinese,and the level of recommendations were evaluated using the Grading of Recommendations Assessment,Development,and Evaluations(GRADE)system. | Xuming Mo Wei Cai Jirong Qi Zhuoming Xu Ying Wang Weihui Yan Shoujun Li Nianguo Dong Xinxin Chen Jinfen Liu Qiang Shu Jimei Chen Haibo Zhang Hao Zhang Quansheng Xing Qi An Xiaofeng Li Xu Wang Yan He Junwu Su Taibing Fan Teng Ming Weibing Tang Li Hong Jinghao Zheng Ming Ye Guocheng Sun Yiqun Ding Liang Tao Yifeng Yang Zhongshi Wu Hua Cao Qiang Wang Keming Yang Libing Zhang Ping Wen Yanqin Cui Bo Zhai Yong Zou Qingya Tang Rui Chen Chun Wu Zhiyu Feng Caixia Liu Yaping Mi Rufang Zhang Ke Lin Xin Li Mingan Pi Xiangming Fan Shanshan Shi Peng Huang Zhengxia Pan Jiafeng Qi Renwei Chen Shuguang Tao Yaqin Shu Huifeng Zhang Lan Jiang Min Da Nishant Patel Liang Hu Cardiac Surgery Group of Pediatric Surgery Society of Chinese Medical Association and Parenteral Enteral Nutrition Society of Chinese Medical Association | 2023 | Congenital Heart Disease2023，18，6： | 0 |
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