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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Herbicide resistant fertile transgenic wheat plant obtained by microprojectile bombardment of regenerable embryogenic callus显示文摘 | Vasil V Castillo A M Fromm M E Vasil I K | 1992 | Bio/Tech1992，10，： | 2 |
| 3 | Genotypic variation in nitrogen use efficiency in medium- and long-duration rice显示文摘 | U Singh J.K Ladha E.G Castillo G Punzalan A Tirol-Padre M Duqueza | 1998 | Field Crops Research1998，，1： | 2 |
| 4 | Decontamination of beef carcass surface tissue by steam vacuuming alone and combined wit h hot water and Iactic acid sprays显示文摘 | Castillo A L ucia L M Goodson K J | 1999 | J Food Prot1999，62，： | 1 |
| 5 | Somatic embryogenesis and plant regenerarion from barley cuhivars grown in spain 显示文摘 | Castillo A Egana B Sanz J M | 1995 | Plant Cell Rep1995，17，： | 1 |
| 6 | Herbicide ersistant regenerable embryogenic callus显示文摘 | Vasil V Castillo A M Fromm M E | 1992 | Bio/Technology1992，10，： | 1 |
| 7 | Agricultural water management and poverty linkages显示文摘 | Namara R E Hanjra M A Castillo G E | 2010 | Agricultural Water Management2010，97，4： | 1 |
| 8 | Laparoscopic resection of adrenal teratoma显示文摘 | CASTILLO O A VITAGLIANO G VILLETA M | 2006 | JSLS2006，10，： | 1 |
| 9 | Factors regulating spatial distribution of soil water content in small semiarid catchments显示文摘 | A Gómez-Plaza M Mart??nez-Mena J Albaladejo V.M Castillo | 2001 | Journal of Hydrology2001，，1： | 1 |
| 10 | In-plant evaluation of a lactic acid treatment for reduction of bacteria on chilled beef carcasses显示文摘 | CASTILLO A LUCIA L M MERCADO I | 2001 | Food Protection2001，，64： | 1 |
| 11 | Diffusion-weighted MR imaging offer no advantage over routine noncontrast MR imaging in the detection of vertebral metastases显示文摘 | Castillo M Arbelaez A Smith JK | 2000 | AJNR2000，21，： | 1 |
| 12 | Influence of salinity on germination and seeds viability of two primary colonizers of Mediterranean salt pans显示文摘 | Rubio-Casal A E Castillo J M Luque C J | 2003 | J Arid Environ2003，53，2： | 1 |
| 13 | Isolated soy protein improves endothelial function in postmenopa- usal hypercholesterolemic women 显示文摘 | Cuevas A M Irribarra V L Castillo 0 A | 2003 | European Journal of Clinical Nutrition2003，57，8： | 1 |
| 14 | The role of antecedent soil water content in the runoff response of semiarid catchments: a simulation approach显示文摘 | Castillo V M Gomez-Plaza A Martinez-Mena M | 2003 | Journal of Hydrology2003，284，： | 1 |
| 15 | Methylenetet- rahyd - Rofolate reductase polymorphism( 677C > T)predicts long time to pro - gression in metastic colon cancer treated with 5 - flu- orouracil and folinic acid 显示文摘 | Castillo - Fernandez O Santibaez M Bauza A | 2010 | Arch Med Res2010，41，6： | 1 |
| 16 | Path planning for autonomous mobile robot navigation with ant colony optimization and fuzzy cost function evaluation显示文摘 | GARCIA M A P MONTIEL O CASTILLO O | 2009 | Applied Soft Computing2009，9，3： | 1 |
| 17 | Predicting eoag-ulation and syneresis parameters of milk gels when inulin is added as fat substitute using infrared light backscatter 显示文摘 | ARANGO O TRUJILLO A J CASTILLO M | 2015 | Journal of Food Engineering2015，157，： | 1 |
| 18 | Acute Marchiafava-Bignami disease:MR findings in two patients显示文摘 | Arbelaez A Pajon A Castillo M | 2003 | AJNR Am J Neuroradiol2003，24，10： | 1 |
| 19 | Diffusion-weighted MR Imaging offers no advantage over routine noncontrast MR Imaging in the detection of vertebral metastases显示文摘 | Castillo M Arbelaez A Simth JK | 2000 | AJNR2000，21，5： | 1 |
| 20 | Influence of salinity on germination and seeds viability of two primary colonizers of Mediterranean salt pans显示文摘 | Rubio-Casal A E Castillo J M Luque C J | 2003 | Journal of Arid Environments2003，53，： | 1 |
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