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4篇 您的检索式:作者名="Chae Oh Lim"
    题名 作者 年代 出处 被引量
1A Proximal Promoter Region of Arabidopsis DREB2C Confers Tissue-specific Expression under Heat Stress显示文摘The dehydration-responsive element-binding factor 2C (DREB2C) is a member of the CBF/DREB subfamily of proteins, which contains a single APETALA2/Ethylene responsive element-binding factor (AP2/ERF) domain. To identify the expression pattern of the DREB2C gene, which contains multiple transcription cis-regulatory elements in its promoter, an approximately 1.4 kb upstream DREB2C sequence was fused to the β-glucuronidase reporter gene (GUS) and the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh. The promoter of the gene directed prominent GUS activity in the vasculature in diverse young dividing tissues. Upon applying heat stress (HS), GUS staining was also enhanced in the vasculature of the growing tissues. Analysis of a series of 5 -deletions of the DREB2C promoter revealed that a proximal upstream sequence sufficient for the tissue-specific spatial and temporal induction of GUS expression by HS is localized in the promoter region between 204 and 34 bps relative to the transcriptional start site. Furthermore, electrophoretic mobility shift assay (EMSA) demonstrated that nuclear protein binding activities specific to a 120 to 32 bp promoter fragment increased after HS. These results indicate that the TATA-proximal region and some latent trans-acting factors may cooperate in HS-induced activation of the Arabidopsis DREB2C promoter.Jihyun Je Chieun Song Jung Eun Hwang Chae Oh Lim 2012Journal of Integrative Plant Biology2012,54,9:4
2Isolation and characterization of two β-tubulin cDNA clones from rice显示文摘Mee Sun Kang Young Ju Choi Min Chul Kim Chae Oh Lim Inhwan Hwang Moo Je Cho 1994Plant Molecular Biology1994,,6:1
3Novel method to dissociate platelet clumps in EDTA-dependent pseudothrombocytopenia based on the pathophysiological mechanism显示文摘Hyojin Chae Myungshin Kim Jihyang Lim Eun-Jee Oh Yonggoo Kim Kyungja Han 2012Clinical Chemistry and Laboratory Medicine2012,,8:1
4Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds显示文摘Chae Oh Lim Soo In Lee Woo Sik Chung Sung Han Park Inhwan Hwang Moo Je Cho 1996Plant Molecular Biology1996,,2:1
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