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您的检索式：作者名="Changrong Deng"
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| 1 | The microgravity enhanced polymer-mediated siRNA gene silence by improving cellular uptake显示文摘Microgravity(MG)effect is a weightlessness phenomenon caused by the distance from the ground or low gravity of other planets outside the earth's atmosphere.The various effects of MG have been corroborated in human and animal studies and modeled in cell-based analogs.However,the impact of MG on siRNA performance remains to be elucidated,which is crucial for aerospace medicine.In this study,we prepared nucleic acid nanomicelles(EAASc/siRNA)by using tri-block copolymer of PEG45-PAMA40-P(C7A36-DBA37)(EAASc)and siRNA and explored its working mechanism under simulated microgravity(SMG)condition generated by a random positioning machine(RPM).The binding ability of EAASc to siRNA and silence activity were firstly confirmed in normal gravity(NG)environment.Evaluation of PLK1 mRNA expression revealed that gene inhibition efficiencies were increased by 28.7%(HepG2)and 28.9%(A549)under SMG condition,compared with those under NG condition.In addition,mechanism exploration indicated that morphology and migration capability of cancer cells were significantly changed,the internalization of EAASc/siRNA by cells was magnified when the cells were incubated with RPM.No significant difference was observed regarding the expression profiles of genes involved in RNA interference(RNAi)pathway,including Ago2,Dicer,TRBP,and so on.Taken together,siRNA activity was elevated under SMG condition owning to increased cellular internalization.This study,for the first time to our knowledge,provides valuable theory for development and application of siRNA therapeutic in space in the future. | Tongren Yang Chanchan Yu Changrong Wang Chunhui Li Mengjie Zhang Xiaofan Luo Yuhua Weng Anjie Dong Xiaoqiong Li Yulin Deng Yuanyu Huang | 2020 | Biophysics Reports2020，6，6： | 1 |
| 2 | Application of BeiDou navigation satellite system in emergency rescue of natural hazards: a case study for field geological survey of Qinghai−Tibet plateau显示文摘In recent years,geological and mineral resources exploration in China has expanded to deep hinterland of the Qinghai−Tibet Plateau and other regions with complex geological conditions.The special natural conditions of Qinghai−Tibet Plateau determine the characteristics of“life-forbidden zone”that is characterized by alpine hypoxia,changeable weather,complex road conditions,and beast attack.In particular,the work in wild depopulated zones with severe environment and poor communications imposes serious threats to the life safety of geological personnel.Therefore,how to guarantee the safety of geological personnel working on the Qinghai−Tibet Plateau and how to reduce or even avoid casualty of geological personnel have currently become the urgent challenge.In this study,an emergency rescue information system for field geological survey is constructed based on BeiDou Navigation Satellite System.A case study of emergency rescue has been conducted in the depopulated zone of the Qinghai−Tibet Plateau and good effects have been achieved,providing security assurance for personnel engaged in field geological survey on the Qinghai−Tibet Plateau and technical support for the emergency rescue in case of natural hazards on the Qinghai−Tibet Plateau.The BeiDou Navigation Satellite System(BDS)can be effectively used to locate and communicate in the emergency rescue for rigorous Geological survey task where there is no network signal for the mobile phone,and the emergency rescue guarantee system is independent,reliable,and relatively cheap.The application value of BDS is demonstrated in the geological field. | Ming Hao Jianlong Zhang Ruiqing Niu Changrong Deng Hong Liang | 2018 | Geo-Spatial Information Science2018，21，4： | 0 |
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