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| 1 | Evolution of quasispecies diversity for porcine reproductive and respiratory syndrome virus under antibody selective pressure显示文摘To study the quasispecies diversity of porcine reproductive and respiratory syndrome virus (PRRSV), open reading frame 5 (ORF5) of strain SD0612 was amplified and cloned. Sixty clones of ORF5 were sequenced and analyzed with DNAStar software. Nucleic acid sequence homology was 97.7%-100%, with 78 mutations observed. Among these 60 clones, the sequences of 17 clones were identical and recognized as the dominant quasispecies of strain SD0612. Evolution of SD0612 quasispecies diversity under antibody selective pressure was also studied. SD0612 was passed continuously in the Marc-145 cell line over 40 passages in 6 independent lineages. SD0612 antiserum was not added to lineage A, B, and C cultures; however, antiserum was added to culture medium for lineages D, E, and F. PRRSV ORF5 was then amplified, cloned, and sequenced from each of the 6 lineages, designated as A40-F40. F40 was further passed in Marc-145 cells using 6 independent lineages with or without F40 antiserum for another 40 passages. ORF5 from the 6 newly-derived virus lineages, which we designated as a40-f40, were amplified, cloned and sequenced. The proportion of dominant quasispecies increased with passage number in cell cultures supplemented with antibodies, but decreased when antibodies were lacking. Our work has demonstrated a diversity of quasispecies for ORF5 in PRRSV SD0612. Antibody selective pressure was able to significantly influence quasispecies diversity and promote a dominant quasispecies that was able to evade immune reactions. | ZHAO Peng MA ChengTai DONG Xuan CUI ZhiZhong | 2012 | Science China(Life Sciences)2012，55，9： | 4 |
| 2 | Crystal Structure of Copper(Ⅱ) Complex with 3-Hydroxyl-1,5-diazacyclooctane显示文摘CrystalStructureofCopper(Ⅱ)Complexwith3-Hydroxyl-1,5-diazacyclooctaneLiuSheng-Hua;XueGuo-Ping;FuEn-Qin;WuChengTai(Departmento... | Liu Sheng-Hua Xue Guo-Ping Fu En-Qin Wu ChengTai(Department of Chemistry, Wuhan University, Wuhan 430072) Luo Bao-Sheng Chen Liao-Rong(Centre of Analysis and Testing, Wuhan University, Wuhan 430072) | 1996 | Chinese Journal of Structural Chemistry1996，15，4： | 2 |
| 3 | A synthetical evaluation about the catastropha-hility of urban geomorphic environment in Chongqing显示文摘 | Diao Chengtai | 1995 | Journal of southwest China Normal University(natural Science)1995，20，4： | 1 |
| 4 | Application of microbial phytase in fish feed 显示文摘 | Ling Cao Weimin Wang Chengtai Yang | 2007 | Enzyme and microbial technology2007，，40： | 1 |
| 5 | Application of microbial phytase in fish feed 显示文摘 | Ling Cao Weimin Wang Chengtai Yang | 2007 | Enzyme and Microbial Technology2007，，40： | 1 |
| 6 | A facile method for the synthesis of hepta-O-acetyl-β-D- lactosyl subtituted benzoates显示文摘 | Zhiqun D Fanqi Q Chengtai W | 2001 | J ChemRes2001，，3： | 1 |
| 7 | Single-Phase Fluid Flow in a Stratified Porous Medium with Crossflow显示文摘 | | | SPE0，，： | 1 |
| 8 | Pressure Transients and Crossflow Caused by Diffusivities in Muitilayer Reservoirs显示文摘 | Deans H A | 1966 | SPE1966，1，： | 1 |
| 9 | Coppre Compex of Hydroxyl-Substituted Triazamacrocyclic Ligand and Antitumor Activity显示文摘 | Feng Liang Chengtai Wu Huakuan Lin | 2003 | Bioorganic & Medicinal Chemistry Letter2003，13，： | 1 |
| 10 | Preparation and coordination behavior of cellulose beads derivative modified by 1,5-diaminoethyl-3-hydroxy-1,5-diazacycloheptane 显示文摘 | He Yongbing Meng Lingzhi Wu Chengtai | 1999 | J Appl Poly Sc1999，74，5： | 1 |
| 11 | Comparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China显示文摘 | Chengtai Yang Ling Cao Weimin Wang Yi Yang Khalid Abbas Binlun Yan Hanping Wang Li Su Yuhua Sun Huanling Wang | 2009 | Environmental Biology of Fishes2009，，1： | 1 |
| 12 | Single-phase fluid flow in a stratfied porous medium with crossflow 显示文摘 | Gao Chengtai | 1984 | Society of Petroleum Engineers Journal1984，24，1： | 1 |
| 13 | Application of microbial phytase in fish feed显示文摘 | CAO Ling WANG Weimin YANG Chengtai | 2007 | Enzyme and Microbial Technology2007，40，4： | 1 |
| 14 | A synthetical evaluation about the catastropha-bility of urban geomorphic environment in Chongqing显示文摘 | Diao Chengtai | 1995 | Journal of Southwest China Normal University(Nature Seience)1995，20，4： | 1 |
| 15 | Application of microbial phytase in fish feed显示文摘 | Cao Ling Wang Weimin Yang Chengtai et a/ | 2007 | Enzyme Microb Technol2007，40，4： | 1 |
| 16 | Application of microbial phytase in fish feed显示文摘 | Ling Cao Weimin Wang Chengtai Yang etc | 2007 | Enzyme and Microbial Technology2007，40，： | 1 |
| 17 | Hyperbaric oxygen therapy ameliorates the blood–retinal barrier breakdown in diabetic retinopathy显示文摘 | Yun‐HsiangChang Po‐LiangChen Ming‐ChengTai Chiao‐HongChen Da‐WenLu Jiann‐TorngChen | 2006 | Clinical & Experimental Ophthalmology2006，，6： | 1 |
| 18 | Synthesis of New Bis-diphenyl-22-and Diphenyl-11-Membered Ring N-pivot lariat Crown Ethers显示文摘SynthesisofNewBis┐diphenyl┐22┐andDiphenyl┐11┐MemberedRingN┐pivotlariatCrownEthersNiuChangrong,LuoTiejun,WuChengtai,HeYongbin... | Niu Changrong Luo Tiejun Wu Chengtai He Yongbin | 1997 | Wuhan University Journal of Natural Sciences1997，2，1： | 0 |
| 19 | Gas Flow in a Stratified Porous Medium with Crossflow显示文摘A new model called semi-permeable wall model is presented for multilayer gas reservoir. The model is used to study the influence of crossflow on pressure transient well tests and other single-phase flow problems. It is suggested here to use this model to approximate the actual multilayer gas reservoir, so that the problem is greatly simplified mathematically. Its differential equation is established here for multilayer gas reservoirs, and is linearized by normalized pseudo pressure and pseudo time. Simulation program is developed by finite-difference method when all layers are perforated. The feature of wellbore pressure and rate is clarified by analyzing the results of numerical simulation. | Sun Hedong Gao Chengtai Qian Huanqun Zhou Fangde | 2002 | Journal of Thermal Science2002，11，1： | 0 |
| 20 | Comprehensive Construction of Innovative Talent Cultivating Model in Colleges and Universities显示文摘Innovation is the soul of progress of a nation and the first driving force for development.Building an innovative country is the core of the national development strategy,which requires a large number of talents with innovative consciousness and ability.Colleges and universities undertake the important mission of cultivating innovative talents,while the cultivating model is related to the quality of innovative talents cultivation.From nine aspects,namely,disciplines,course systems,teaching methods,teacher staff,practical links,scientific and technological activities,innovation platforms,system guarantees,and party building,this study explained the construction of innovative talent cultivation model,so as to provide a certain reference for the cultivation of innovative talents in colleges and universities. | Rongan CAO Chengtai ZHANG Yinghua LUO Jiamiao ZHANG Guinan SHEN Chenghao JIN | 2021 | Asian Agricultural Research2021，13，4： | 0 |
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