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| 1 | Microfluidic actuators based on temperature-responsive hydrogels显示文摘The concept of using stimuli-responsive hydrogels to actuate fluids in microfluidic devices is particularly attractive,but limitations,in terms of spatial resolution,speed,reliability and integration,have hindered its development during the past two decades.By patterning and grafting poly(N-isopropylacrylamide)PNIPAM hydrogel films on plane substrates with a 2μm horizontal resolution and closing the system afterward,we have succeeded in unblocking bottlenecks that thermo-sensitive hydrogel technology has been challenged with until now.In this paper,we demonstrate,for the first time with this technology,devices with up to 7800 actuated micro-cages that sequester and release solutes,along with valves actuated individually with closing and opening switching times of 0.6±0.1 and 0.25±0.15 s,respectively.Two applications of this technology are illustrated in the domain of single cell handling and the nuclear acid amplification test(NAAT)for the Human Synaptojanin 1 gene,which is suspected to be involved in several neurodegenerative diseases such as Parkinson’s disease.The performance of the temperature-responsive hydrogels we demonstrate here suggests that in association with their moderate costs,hydrogels may represent an alternative to the actuation or handling techniques currently used in microfluidics,that are,pressure actuated polydimethylsiloxane(PDMS)valves and droplets. | Loïc D'Eramo Benjamin Chollet Marie Leman Ekkachai Martwong Mengxing Li Hubert Geisler Jules Dupire Margaux Kerdraon Clémence Vergne Fabrice Monti Yvette Tran Patrick Tabeling | 2018 | Microsystems & Nanoengineering2018，4，1： | 3 |
| 2 | Functional reorganization of the brain in recovery from striatocapsular infarction in man 显示文摘 | Weiller C Chollet F Friston K | 1992 | Ann Neurol1992，31，： | 2 |
| 3 | Immunocytochemical characterization of pulmonary histiocytosis X cells in lung biopsies显示文摘 | Soler P Chollet S Jacque C | 1985 | Am J Pathol1985，118，3： | 1 |
| 4 | Hypo-oxygenation in paraquat poisoning: Apropos of 6 cases 显示文摘 | Chollet A Muszynsky J Bismuth C | 1983 | Toxicol Eur Res1983，5，： | 1 |
| 5 | In vitro antileishmanial activity of fluoro artemisinin derivatives against Leishmania donovani 显示文摘 | CHOLLET C CROUSSE B BORIES C | 2008 | Biol Med Pharmacother2008，62，7： | 1 |
| 6 | mar operon involvedin multidrug resistance of Enterobacter aero genes显示文摘 | Chollet R Bollet C Chevalier J | 2002 | Antimicrob Agents Chemother2002，46，4： | 1 |
| 7 | Functional reorganization of thebrain in recovery from striatocapsular infarction in man显示文摘 | Weiller C Chollet F Friston KJ | 1992 | Ann Neurol1992，，31： | 1 |
| 8 | Selective indirect electroreduction of 5-(nitrophenyl)-1,2-dithiole-3-thiones显示文摘 | CHOLLET M BURGOT J MOINET C | 1988 | Electrochimica Acta1988，15，： | 1 |
| 9 | Improvement in solubility and dissolution rate of 1,2-Dithiole-3-thiones upon eomplexation with b-Cyelodextrin and its hydroxypropyl and sulfobutyl ether-7 derivatives 显示文摘 | Dollo G Le C P Chollet M | 1999 | Journal of Pharmaceutical Sciences1999，88，9： | 1 |
| 10 | Regulation of pyruvate, orthophosphate dikinase by ADP -/Pi - dependent reversible phosphorylation in C3 and C4 plants 显示文摘 | CHASTAIN C J CHOLLET R | 2003 | Plant Physiol Bioehem2003，41，67： | 1 |
| 11 | Kallikrein protects against microalbuminuria in experimental type I diabetes显示文摘 | Bodin S Chollet C Goncalves-Mendes N | 2009 | Kidney Int2009，76，4： | 1 |
| 12 | Imipenem and expression of multidrug efflux pump in Enterobacter aerogenes显示文摘 | Bornet C Chollet R Mallea M | 2003 | Biochem Biophys Res Commun2003，301，4： | 1 |
| 13 | The effect of RGD density on osteoblast and endothelial cell behavior on RGD-grafted polyethylene terephthalate surfaces 显示文摘 | Chollet C Chanseau C Remy M | 2009 | Biomaterials2009，30，： | 1 |
| 14 | Functional reorganization of the brain in recovery from striatocapsular infarction in man显示文摘 | Weiller C Chollet F Friston K J | 1992 | Ann Neurol1992，31，5： | 1 |
| 15 | A quality control program for acute pain management in out-of-hospital critical care medicine显示文摘 | RicardHibon A Chollet C Saada S | 1999 | Ann Emerg Med1999，34，8： | 1 |
| 16 | Indirect electroreduction of 5 - (nitrophenyl) - 1,2 - dithiole - 3 - thiones 显示文摘 | Chollet M Burgot J L Moinet C | 1998 | Electrochimica Acta1998，44，23： | 1 |
| 17 | Thermal valley inversion impact on the dispersion of a passive pollutant in a complex mountainous area显示文摘 | ANQUET1N S GUILBAUD C CHOLLET J P | 1999 | Atmos Environ1999，33，2425： | 1 |
| 18 | p53 status and efficacy of primary anthracyclines/alkylating agent based regimen according to breast cancer molecular classes显示文摘 | Bidard F C Matthieu M C Chollet P | 2008 | Ann Oncol2008，19，7： | 1 |
| 19 | Stroking characteristic variations in the 100-m freestyle for male swimmers of differing skill 显示文摘 | CHOLLET D PELAYO P DELAPLACE C | 1997 | Perceptual Motor Skills1997，85，1： | 1 |
| 20 | Comparative analysis of 100m and 200m events in the four strokes in top level swimmers显示文摘 | CHOLLET D PELAYO P TOURNY C | 1996 | J Human Move Studies1996，31，1： | 1 |
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