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| 1 | The treat- ment of open tibial fractures and of tibial non - union with a novel external fixator 显示文摘 | Ong C T Choon D S K Cabrera N P | 2002 | Injur2002，33，9： | 1 |
| 2 | Fatigue strength evaluation of aluminum carbody of urban transit unit by large scale dynamic load test 显示文摘 | Sung I S Choon P Kin K H | 2005 | JSME international journal2005，48，1： | 1 |
| 3 | Fatigue strength evaluation of the aluminum earbody of urban transit unit by large scale dynamic load test 显示文摘 | Sung I S Choon P Kim K H | 2005 | JSME International Journal2005，48，1： | 1 |
| 4 | Heuristic procedures for muhi-item inventory planning with limited storage 显示文摘 | Paul S D Choon L P | 1990 | IIE Transactions1990，22，2： | 1 |
| 5 | Growth and characterisation of CsLiB6Oi0 (CLBO) crystals显示文摘 | RYU Gihan YOON Choon Sup HAN Thomas P J | 1998 | Journal of Crystal Growth1998，191，3： | 1 |
| 6 | Heuristic procedures for multi-item inventory planning with limited storage显示文摘 | Paul S D Choon L P | 1990 | IIE Transactions1990，22，2： | 1 |
| 7 | Increased levels of interleukin-6 are associated with lymphocytosis in bronchoalveolar lavage fluids of idiopathic nonspecific interstitial pneumonia 显示文摘 | CHOON S P SUNG W C SHIN Y K | 2000 | Am J Respir Crit Care Med2000，162，： | 1 |
| 8 | Acoustic surperlens using membrane-based metamaterials 显示文摘 | JONG J P CHOON M P LEE K J B | 2015 | Appl Phys Lett2015，106，05： | 1 |
| 9 | Defluoridation from aqueous solution by lanthanum hydroxide显示文摘 | CHOON K N HYUN J P | 2010 | Journal of Hazardous Materials2010，183，： | 1 |
| 10 | Exchange Rates and the Trade Balance: the Case of Singapore 1970 to 1996显示文摘 | Wilson P Choon Tat Kua | 2002 | Journal of Asian Economics2002，12，： | 1 |
| 11 | The treatment of open tibial fractures and tibial non-union with a novel external fixator 显示文摘 | Ong C T Choon D S Cahrera N P | 2002 | Injury2002，，33： | 1 |
| 12 | Generation of a human embryonic stem cell line stably expressing high levels of the fluorescent protein mCherry显示文摘AIM:The generation and characterization of a human embryonic stem cell (hESC) line stably expressing red fluorescent mCherry protein. METHODS:Lentiviral transduction of a ubiquitously-expressed human EF-1α promoter driven mCherry transgene was performed in MEL2 hESC. Red fluore-scence was assessed by immunofluorescence and flow cytometry. Pluripotency of stably transduced hESC was determined by immunofluorescent pluripotency marker expression, flow cytometry, teratoma assays andembryoid body-based differentiation followed by reverse transcriptase-polymerase chain reaction. Quantification of cell motility and survival was performed with time lapse microscopy. RESULTS:Constitutively fluorescently-labeled hESCs are useful tools for facile in vitro and in vivo tracking of survival, motility and cell spreading on various surfaces before and after differentiation. Here we describe the generation and characterization of a hESC line (MEL2) stably expressing red fluorescent protein, mCherry. This line was generated by random integration of a fluorescent protein-expressing cassette, driven by the ubiquitously-expressed human EF-1α promoter. Stably transfected MEL2-mCherry hESC were shown to express pluripo-tency markers in the nucleus (POU5F1/OCT4, NANOG and SOX2) and on the cell surface (SSEA4, TRA1-60 and TG30/CD9) and were shown to maintain a normal karyotype in long-term (for at least 35 passages) culture. MEL2-mCherry hESC further readily differentiated into representative cell types of the three germ layers in embryoid body and teratoma based assays and, importantly, maintained robust mCherry expression throughout differentiation. The cell line was next adapted to singlecell passaging, rendering it compatible with numerous bioengineering applications such as measurement of cell motility and cell spreading on various protein modified surfaces, quantification of cell attachment to nanoparticles and rapid estimation of cell survival. CONCLUSION:The MEL2-mCherry hESC line conforms to the criteria of bona fide pluripotent stem cells and maintains red fluorescence throughout differentiation, making it a useful tool for bioengineering and in vivo tracking experiments. | Dmitry A Ovchinnikov Jennifer P Turner Drew M Titmarsh Nilay Y Thakar Dong Choon Sin Justin J Cooper-White Ernst J Wolvetang | 2012 | World Journal of Stem Cells2012，4，7： | 1 |
| 13 | Polyphenoloxidases in Jerusalem artichoke显示文摘 | MI-JEONG K CHOON Y K INSHIK P | 2005 | Food Chemistry2005，89，： | 1 |
| 14 | Preparation and characterization of polyethylene glycol modi fled salmon calcitonins preparation and characterization of polyethylene glycol-modified salmon calcitonins显示文摘 | Kang Choon L Kyung Kook T Myung Ok P | 1999 | Pharmaceutical Development and Technology1999，4，2： | 1 |
| 15 | Efficient risk taking and regulatory covenant enforcement in a deregulated banking indus- try显示文摘 | De Young R Hughes J P Choon Geol Moon | 2001 | Journal of Economics and Business2001，53，： | 1 |
| 16 | Breast cancer stem ceils: an up- date显示文摘 | Iqbal J Choon P Y Tan P H | 2013 | J Clin Pathol2013，66，6： | 1 |
| 17 | Composite Acoustic Medium with Simultaneously Negative Den- sity and Modulus显示文摘 | L SAM HYEON P CHOON MAHN SY MUN | 2010 | Physical Review Letters2010，104，5： | 1 |
| 18 | Gas Separation Properties of Polysiloxane/Polyether Mixed Soft Segment Urethane Urea Membrane显示文摘 | Bum P Ho Ki Kim Choon Moo Lee Young | | 0，，01： | 1 |
| 19 | Are Scale Economies in Banking Elusive or Illusive? Evidence Obtained by Incorporating Capital Structure and Risk-taking into Models of Bank Production 显示文摘 | Joseph P Hughes Loretta J Mester Choon Geol Moon | 2001 | Jouranl of Banking & Finance2001，25，： | 1 |
| 20 | Fatigue strength evaluation of aluminum car body of urban transit unit by large scale dynamic load test显示文摘 | Sung I S Choon P Kin K H etal | 2005 | JSME International Journal2005，48，1： | 1 |
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