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| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | Sertoli-Leydig cell tumor of the ovary, a rare cause of precocious puberty in a 12-month-old infant 显示文摘 | CATHERINE S CHOON G PETER J | 2002 | The Journal of Clinical Endocrinology & Metabolism2002，87，1： | 1 |
| 3 | The treat- ment of open tibial fractures and of tibial non - union with a novel external fixator 显示文摘 | Ong C T Choon D S K Cabrera N P | 2002 | Injur2002，33，9： | 1 |
| 4 | Fatigue strength evaluation of aluminum carbody of urban transit unit by large scale dynamic load test 显示文摘 | Sung I S Choon P Kin K H | 2005 | JSME international journal2005，48，1： | 1 |
| 5 | Active control of tip clearance flow in axial compressors显示文摘 | Jin Woo Bae Kenneth S Breuer Choon S Tan | 2005 | ASME Journal of Turbomachinery2005，127，4： | 1 |
| 6 | Fatigue strength evaluation of the aluminum earbody of urban transit unit by large scale dynamic load test 显示文摘 | Sung I S Choon P Kim K H | 2005 | JSME International Journal2005，48，1： | 1 |
| 7 | Heuristic procedures for muhi-item inventory planning with limited storage 显示文摘 | Paul S D Choon L P | 1990 | IIE Transactions1990，22，2： | 1 |
| 8 | Credibility of electronic word-of-mouth:informational and normative determinants of on-line consumer recommendations显示文摘 | Man C Chuan L Choon S | 2009 | International Journal of Electronic Commerce2009，13，4： | 1 |
| 9 | Synthesis of lead zirconate powders via a polyaniline-mediated microemulsion processing route显示文摘 | Fang J Y Wang J Choon S | 1998 | Mater Lett1998，36，： | 1 |
| 10 | Mineralization of osteoblasts with electrospun eollagerdhydroxyapatite nanofibers 显示文摘 | Venugopal J Low S Choon AT | 2008 | J Mater Sci Mater Med2008，19，5： | 1 |
| 11 | Accurate and scalable RF interconnect model for silicon-based RFIC applications显示文摘 | Choon B S Beng H O Kiat S Y | 2005 | IEEE Trans Microw Theo and Tech2005，53，9： | 1 |
| 12 | Heuristic procedures for multi-item inventory planning with limited storage显示文摘 | Paul S D Choon L P | 1990 | IIE Transactions1990，22，2： | 1 |
| 13 | Segmental pedicle screw fixation in the treatment of thoracic idiopathic scoliosis显示文摘 | Suk S Choon K L Won-Joong K | 1995 | Spine1995，20，16： | 1 |
| 14 | Increased levels of interleukin-6 are associated with lymphocytosis in bronchoalveolar lavage fluids of idiopathic nonspecific interstitial pneumonia 显示文摘 | CHOON S P SUNG W C SHIN Y K | 2000 | Am J Respir Crit Care Med2000，162，： | 1 |
| 15 | Relational archetypes, organizational learning, and value creation: Extending the human resource architecture 显示文摘 | Sung- Choon Kang Morris Shad S Snell Scott A | 2007 | Academy of Management Review2007，32，1： | 1 |
| 16 | New Pool Boiling Data for Water With Copper-Foam Metal at Sub- Atmospheric Pressures: Experiments and Correlation 显示文摘 | Choon N K Chakraborty A Aye S M | 2006 | Applied Thermal Engineering2006，26，： | 1 |
| 17 | Interaction of cells andnanofiber scaffolds in tissue engineering 显示文摘 | Venugopal J Low S Choon A T | 2008 | Journal of BiomedicalMaterials Research Part B: Applied Biomaterials2008，84，： | 1 |
| 18 | Calibration of a wind farm wind speed model with incomplete wind data显示文摘 | HOSSEIN H S CHOON Y T JOHN N J | 2014 | IEEE Trans on Sustainable Energy2014，5，1： | 1 |
| 19 | Synthesis of lead zirconate powders via a polyaniline-mediated microemulsion-processing route显示文摘 | Fang J Wang J Choon S | 1998 | Materials Letters1998，36，： | 1 |
| 20 | Magnetic and electrical properties of polypyrrole-coated γ -Fe2O3 nanocomposite particles显示文摘 | Yang X T Xu L G Choon N S | 2003 | Nanotechnology2003，14，： | 1 |
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