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| 1 | Measurement of calprotectin in ascitic fluid to identify elevated polymorphonuclear cell count显示文摘AIM: To evaluate the diagnostic capability of calprotectin in ascitic fluid for detecting a polymorphonuclear (PMN) cell count > 250/μL ascites. METHODS: In this prospective observational study, a total of 130 ascites samples were analysed from 71 consecutive patients referred for paracentesis. Total and differential leukocyte cell counts were determined manually with a Neubauer chamber and gentianviolet stain. Calprotectin was measured in 1 mL ascetic fluid by enzyme-linked immunosorbent assay (ELISA) and a point-of-care (POC) lateral flow assay with the Quantum Blue Reader (Bühlmann Laboratories). All measurements were carried out in a central laboratory by senior personnel blinded to patient history. A PMN count > 250/μL was the primary endpoint of the study. The diagnostic value of ascitic calprotectin measurement was assessed by comparing to the final diagnosis of each patient that had been adjudicated by investigators blinded to calprotectin values. RESULTS: The PMN count was > 250/μL in 19 samples (14.6%) from 15 patients (21.1%) and varied widely among the study population (range 10-19 800/mL and 1-17 820/mL, respectively). Spontaneous bacterial peritonitis (SBP) was the final diagnosis in four patients (5.6%). All patients with PMN ≤ 250/μL had negative bacterial culture. PMN count was elevated in five patients with peritoneal carcinomatosis, three with lymphoma, one with neuroendocrine carcinoma, and two with secondary peritonitis due to abdominal perforation. PMN cell counts correlated with ascitic calprotectin values (Spearman's rho; r = 0.457 for ELISA, r = 0.473 for POC). A considerable range of ascitic calprotectin concentrations was detected by ELISA [median 0.43 μg/mL, interquartile range (IQR) 0.23-1.23 (range 0.10-14.93)] and POC [median 0.38 μg/mL, IQR 0.38-0.56 (range 0.38-13.31)]. Ascitic calprotectin levels were higher in samples with PMN > 250/μL, by both ELISA [median (IQR) 2.48 μg/mL (1.61-3.65) vs 0.10 μg/mL (0.10-0.36), P < 0.001] and POC [2.78 μg/mL (2.05-5.37) vs 0.38 μg/mL (0.38-0.41), P < 0.001]. The area under the receiver operating characteristics curve for identifying an elevated PMN count was 0.977 (95%CI: 0.933 to 0.995) for ELISA and 0.982 (95%CI: 0.942 to 0.997) for POC (P = 0.246 vs ELISA). Using the optimal cut-off value for ELISA (0.63 μg/mL), ascitic calprotectin had 94.8% sensitivity, 89.2% specificity, positive and negative likelihood ratios of 8.76 and 0.06 respectively, positive and negative predictive values of 60.0% and 99.0% respectively, and 90.0% overall accuracy. Using the optimal cut-off value for POC (0.51 μg/mL), the respective values were 100.0%, 84.7%, 6.53, 0.00, 52.8%, 100% and 87.7%. Correlation between ELISA and POC was excellent (r = 0.873, P < 0.001). The mean ± SD of the difference was -0.11 ± 0.48 μg/mL with limits of agreement of + 0.8 μg/mL (95%CI: 0.69 to 0.98) and -1.1 μg/mL (95%CI: -1.19 to -0.91). CONCLUSION: Ascitic calprotectin reliably predicts PMN count > 250/μL, which may prove useful in the diagnosis of SBP, especially with a readily available bedside testing device. | Emanuel Burri Felix Schulte Jürgen Muser Rémy Meier Christoph Beglinger | 2013 | World Journal of Gastroenterology2013，19，13： | 10 |
| 2 | Predictors of outcome in patients with normal-pressure hydrocephalus显示文摘 | Ullrich Meier Christoph Miethke | 2003 | Journal of Clinical Neuroscience2003，10，4： | 1 |
| 3 | Utliziation of the less invasive stabilization system internal fixation for open fractures of the proximal tibia:a multi-center evaluation显示文摘 | James P Stannard Christopher G Finke Meier Jackson Lee | 2008 | Indian J Orthop2008，42，4： | 1 |
| 4 | The promyelocytic leukemia protein does not mediate foamy virus latency in vitro显示文摘 | Christopher D Meiering Maxine L Linial | 2003 | Journal of Virology2003，2，： | 1 |
| 5 | Seizures in patients with Alzheimer’s disease or vascular dementia: A population‐based nested case–control analysis显示文摘 | Patrick Imfeld Michael Bodmer Markus Schuerch Susan S. Jick Christoph R. Meier | 2012 | Epilepsia2012，，： | 1 |
| 6 | Compartment Pressure of the Rectus Sheath Accurately Reflects Intra-Abdominal Pressure in a Porcine Model显示文摘 | Emanuel Benninger Matthias W. Laschke Markus Cardell Joerg H. Holstein Burkhardt Seifert Marius Keel Otmar Trentz Michael D. Menger Christoph Meier | 2010 | Journal of Surgical Research2010，，2： | 1 |
| 7 | vibrational excitation Laser in carboxyhemoglobin: A quantum wave packet study 显示文摘 | Meier Christoph control of Heitz Marie-Catherine | 2005 | The Journal of Chemical Physics2005，123，04： | 1 |
| 8 | Risk of colorectal cancer after initiation of orlistat: matched cohort study显示文摘 | Jin-Liern Hong Christoph R Meier Robert S Sandler Susan S Jick Til Stürmer | 2013 | BMJ . 2013 (aug2)2013，，2： | 1 |
| 9 | Chest injuries - what is new显示文摘 | Marius Keel Christoph Meier | 2007 | Current Opinion in Critical Care2007，13，： | 1 |
| 10 | Clinical Comparison of Two Optional Vena Cava Filters显示文摘 | Isabella S. Keller Christoph Meier Roger Pfiffner Emanuela Keller Thomas Pfammatter | 2007 | Journal of Vascular and Interventional Radiology2007，，： | 1 |
| 11 | Novel technique for endoscopic en bloc resection(EMR+)-Evaluation in a porcine model显示文摘BACKGROUND Endoscopic en bloc resection of larger polyps is relevant because risk of advanced neoplasia or malignancy correlates with tumor size. Recurrence rates after piecemeal endoscopic mucosal resection (EMR) are high and endoscopic submucosal dissection (ESD) is associated with higher complication rates in the western world. AIM To develop a modified endoscopic en bloc resection technique using an external additional working channel and novel agent for submucosal injection. METHODS EMR+ was considered as modified grasp and snare technique. For simultaneous use of a grasping and cutting device a novel additional working channel was used (AWC■, Ovesco Endoscopy, Tübingen, Germany). AWC■ is installed on the outer surface of the endoscope, covered with a plastic sleeve and designed for single use. For submucosal injection a new agent consisting of poloxamers was used (LiftUp■, Ovesco Endoscopy, Tübingen, Germany). The agent is liquid at room temperature and forms a stable and permanent gel cushion after injection. Safety of LiftUp? has been shown in a pre-clinical study in domestic pigs. LiftUp■ is commercially not yet available but approval is expected in early 2019. EMR+ was first developed ex vivo (explanted pig stomach) and subsequently evaluated in vivo (stomach, porcine model, 3 domestic pigs). Main outcome measurements were: Procedure time, macroscopic en bloc resection and adverse events. RESULTS Concept of EMR+ was first developed ex vivo (explanted pig stomach). Ex vivo, 22 resections were performed after technique was established. Median procedure time (measured from begin of injection to extraction of resection specimen) was 7 min (range 5-11, SD 1.68) and median size of resection specimens was 30 mm × 26 mm × 11 mm ex vivo. Subsequently 13 resections were performed in vivo (stomach, porcine model, 3 domestic pigs). In vivo, median procedure time (measured from begin of injection to extraction of resection specimen) was 5 min (range 3-12, SD 2.72) and median size of resection specimens was 35 mm × 35 mm × 11 mm. In vivo, resection was macroscopic complete in 92.3%, major adverse events were not observed. In one case (7.7%) minor periprocedural bleeding was observed and managed by coagulation. CONCLUSION EMR+ appeared to be effective and safe and was easy and fast to perform in the porcine model. EMR+ needs to be further evaluated clinically in comparative trials. | Benjamin Meier Andreas Wannhoff Christoph Klinger Karel Caca | 2019 | World Journal of Gastroenterology2019，25，28： | 1 |
| 12 | Association Between Extracellular Matrix Expansion Quantified by Cardiovascular Magnetic Resonance and Short-Term Mortality显示文摘 | Timothy C. Wong Kayla Piehler Christopher G. Meier Stephen M. Testa Amanda M. Klock Ali A. Aneizi Jonathan Shakesprere Peter Kellman Sanjeev G. Shroff David S. Schwartzman Suresh R. Mulukutla Marc A. Simon Erik B. Schelbert | 2012 | Circulation2012，，10： | 1 |
| 13 | The Adjustable proGAV Shunt: A Prospective Safety and Reliability Multicenter Study显示文摘 | Christian Sprung Hans-Georg Schlosser Johannes Lemcke Ullrich Meier Martina Messing-Jünger Hans Axel Trost Friedrich Weber Christoph Schul Veit Rohde Hans-Christian Ludwig Jürgen H?pfner Abolghassem Sepehrnia M. Javad Mirzayan Joachim K. Krauss | 2010 | Neurosurgery2010，，3： | 1 |
| 14 | Extent of coronary artery disease and outcomes after ticagrelor administration in patients with an acute coronary syndrome: Insights from the PLATelet inhibition and patient Outcomes (PLATO) trial显示文摘 | Anna Kotsia Emmanouil S. Brilakis Claes Held Christopher Cannon Gabriel P. Steg Bernhard Meier Frank Cools Marc J. Claeys Jan H. Cornel Philip Aylward Basil S. Lewis Douglas Weaver Gunnar Brandrup-Wognsen Susanna R. Stevens Anders Himmelmann Lars Wallenti | 2014 | American Heart Journal2014，，： | 1 |
| 15 | Case–control analysis on metformin and cancer of the esophagus显示文摘 | Claudia Becker Christoph R. Meier Susan S. Jick Michael Bodmer | 2013 | Cancer Causes & Control2013，，10： | 1 |
| 16 | Comparison of abdominal aortic aneurysm diameter measurements obtained with ultrasound and computed tomography: is there a difference?显示文摘 | L.Richard Sprouse George H Meier Christopher J LeSar Richard J DeMasi Jaideep Sood F.Noel Parent Michael J Marcinzyck Robert G Gayle | 2003 | Journal of Vascular Surgery2003，，： | 1 |
| 17 | Evaluation of a handheld creatinine measurement device for real-time determination of serum creatinine in radiology departments显示文摘AIM: To assess the feasibility/accuracy of a commercial handheld device in the context of increased demand for point-of-care serum creatinine (SCr) determination. METHODS: In this institutional review board-approved, prospective study, 401 patients referred for contrastenhanced computed tomography were included at two centres. Capillary (c)SCr was determined using two devices A+B and venous (v)SCr was determined in the centre's laboratory. Method comparison statistics for both centres and for vSCr<>1.2 mg/dL, receiver operating characteristic analysis, negative predictive values (NPV), sensitivity and specificity were calculated pre-/ post-curve offset correction with vSCr. RESULTS: Pearson's coefficients for cSCr vs vSCr were: centre 1-A:0.93/B:0.92; centre 2-A:0.85/B:0.82 (all P < 0.0001). Overall correlation was better for vSCr > 1.2 mg/dL. The area under the receiver operating characteristic curves showed a high accuracy for cSCr, but the device underestimated SCr, which was confirmed by Bland-Altman plot. Addition of the offset correction factor to the original data from centre 1 resulted in an improvement in sensitivity for detecting patients at risk (> 1.2 mg/dL), whilst maintaining acceptable specificity and improving NPV. CONCLUSION: This study showed the feasibility of SCr determination using the evaluated handheld device in a routine clinical setting. The device showed high sensitivity and high NPV, but may significantly underestimate SCr without offset correction to local laboratories. | Stefan Haneder Alexandra Gutfleisch Claudia Meier Joachim Brade Dieter Hannak Stefan O Schoenberg Christoph R Becker Henrik J Michaely | 2012 | World Journal of Radiology2012，4，7： | 0 |
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