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| 1 | SNP-based QTL mapping for panicle traits in the japonica super rice cultivar Liaoxing 1显示文摘Panicle traits directly associated with yield are a target of selection in rice breeding.Although abundant QTL for panicle traits have been identified,there is little information about the genetic basis of panicle traits in japonica super rice(JSR)cultivars.In this study,we identified QTL for panicle traits in three environments using a population of recombinant inbred lines(RILs)derived from the JSR cultivar Liaoxing 1.A total of 197 RILs were genotyped with 285 polymorphic SNP markers.Phenotypic data and best linear unbiased prediction(BLUP)value of primary branch number(BNP),secondary branch number(BNS),grain number on primary branch(GNP),grain number on secondary branch(GNS),grain number per panicle(GN),panicle length(PL)and grain density(GD)were used for QTL mapping.A total of 105 QTL for seven panicle traits were detected in single environments using their BLUP values.Individual QTL explained 0.51%–52.22%of the phenotypic variation.Of the 105,49 were also detected by joint multi-environment analyses.Five stable QTL:qGD9,qPL9,qGNP9,qGN6,and qBNS6.2 were identified in multiple environments.qGD9,qGNP9,and qPL9,co-localizing on chromosome 9,likely correspond to the known gene DEP1.Importantly,qGN6 and qBNS6.2 in a co-localization region were identified as novel QTL,and their Liaoxing 1 alleles had a positive effect.Several RILs with the QTL allele combinations qGD9/qPL9/qGNP9 and qGN6/qBNS6.2 showed greater GN.Further investigation of the putative gene underlying qGN6/qBNS6.2 would shed light on the molecular mechanism of JSR. | Mingzhu Zhao Zuobin Ma Lili Wang Zhiqiang Tang Ting Mao Chuanbin Liang Hong Gao Liying Zhang Na He Liang Fu Changhua Wang Guomin Sui Wenjing Zheng | 2020 | The Crop Journal2020，8，5： | 4 |
| 2 | Effect of ball milling time on the microstructure and compressive properties of the Fe–Mn–Al porous steel显示文摘In the present work,Fe–Mn–Al–C powder mixtures were manufactured by elemental powders with different ball milling time,and the porous high-Mn and high-Al steel was fabricated by powder sintering.The results indicated that the powder size significantly decreased,and the morphology of the Fe powder tended to be increasingly flat as the milling time increased.However,the prolonged milling duration had limited impact on the phase transition of the powder mixture.The main phases of all the samples sintered at 640℃ were α-Fe,α-Mn and Al,and a small amount of Fe2Al5 and Al8Mn5.When the sintering temperature increased to 1200℃,the phase composition was mainly comprised of γ-Fe and α-Fe.The weight loss fraction of the sintered sample decreased with milling time,i.e.,8.3wt% after 20 h milling compared to15.3wt% for 10 h.The Mn depletion region(MDR) for the 10,15,and 20 h milled samples was about 780,600,and 370 μm,respectively.The total porosity of samples sintered at 640℃ decreased from ~46.6vol% for the 10 h milled powder to ~44.2vol% for 20 h milled powder.After sintering at 1200℃,the total porosity of sintered samples prepared by 10 and 20 h milled powder was ~58.3vol% and ~51.3vol%,respectively.The compressive strength and ductility of the 1200℃ sintered porous steel increased as the milling time increased. | Lingzhi Xie Zhigang Xu Yunzhe Qi Jinrong Liang Peng He Qiang Shen Chuanbin Wang | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，5： | 0 |
| 3 | Characterization of 2,2’,4,4’-tetrabromodiphenyl ether(BDE47)-induced testicular toxicity via single-cell RNA-sequencing显示文摘Background:The growing male reproductive diseases have been linked to higher exposure to certain environmental compounds such as 2,2,4,4-tetrabromodiphenyl ether(BDE47)that are widely distributed in the food chain.However,the specific underlying molecular mechanisms for BDE47-induced male reproductive toxicity are not completely understood.Methods:Here,for the first time,advanced single-cell RNA sequencing(ScRNA-seq)was employed to dissect BDE47-induced prepubertal testicular toxicity in mice from a pool of 76859 cells.Results:Our ScRNA-seq results revealed shared and heterogeneous information of differentially expressed genes,signaling pathways,transcription factors,and ligands-receptors in major testicular cell types in mice upon BDE47 treatment.Apart from disruption of hormone homeostasis,BDE47 was discovered to downregulate multiple previously unappreciated pathways such as double-strand break repair and cytokinesis pathways,indicative of their potential roles involved in BDE47-induced testicular injury.Interestingly,transcription factors analysis of ScRNA-seq results revealed that Kdm5b(lysine-specific demethylase 5B),a key transcription factor required for spermatogenesis,was downregulated in all germ cells as well as in Sertoli and telocyte cells in BDE47-treated testes of mice,suggesting its contribution to BDE47-induced impairment of spermatogenesis.Conclusions:Overall,for the first time,we established the molecular cell atlas of mice testes to define BDE47-induced prepubertal testicular toxicity using the ScRNA-seq approach,providing novel insight into our understanding of the underlying mechanisms and pathways involved in BDE47-associated testicular injury at a single-cell resolution.Our results can serve as an important resource to further dissect the potential roles of BDE47,and other relevant endocrine-disrupting chemicals,in inducing male reproductive toxicity. | Wei Zhang Siyu Xia Xiaoru Zhong Guoyong Gao Jing Yang Shuang Wang Min Cao Zhen Liang Chuanbin Yang Jigang Wang | 2022 | Precision Clinical Medicine2022，5，3： | 0 |
| 4 | Interactions of phenol and benzaldehyde in electrocatalytic upgrading process显示文摘Biomass pyrolysis oil can be improved effectively by electrocatalytic hydrogenation(ECH).However,the unclear interactions among different components lead to low bio-oil upgrading efficiency in the conversion process.Herein,benzaldehyde and phenol,as common compounds in bio-oil,were chosen as model compounds.The interactions between the two components were explored in the ECH process by combining experiments and theoretical calculations.Results showed that phenol could accelerate the conversion of benzaldehyde in the ECH.The selectivity of benzyl alcohol was increased from 60.9%of unadded phenol to 99.1%with 30 mmol/L phenol concentration at 5 h.Benzaldehyde inhibited the ECH of phenol.In the presence of benzaldehyde,the conversion rate of phenol was below 10.0%with no cyclohexanone and cyclohexanol formation at 5 h.The density functional theory(DFT)calculations revealed that the phenol could promote the adsorption of benzaldehyde and facilitate the targeted conversion of benzaldehyde on the active site by lowering the reaction energy barrier.The research on the interaction between phenol and benzaldehyde in the ECH provides a theoretical basis for the application of ECH in practical bio-oil upgrading. | Lan Liang Chuanbin Wang Xukai Lu Yunan Sun Beibei Yan Ning Li Guanyi Chen Li'an Hou | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
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