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| 1 | Plant abiotic stress response and nutrient use efficiency显示文摘Abiotic stresses and soil nutrient limitations are major environmental conditions that reduce plant growth,productivity and quality.Plants have evolved mechanisms to perceive these environmental challenges,transmit the stress signals within cells as well as between cells and tissues,and make appropriate adjustments in their growth and development in order to survive and reproduce.In recent years,significant progress has been made on many fronts of the stress signaling research,particularly in understanding the downstream signaling events that culminate at the activation of stress-and nutrient limitation-responsive genes,cellular ion homeostasis,and growth adjustment.However,the revelation of the early events of stress signaling,particularly the identification of primary stress sensors,still lags behind.In this review,we summarize recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling and discuss new directions for future studies. | Zhizhong Gong Liming Xiong Huazhong Shi Shuhua Yang Luis R.Herrera-Estrella Guohua Xu Dai-Yin Chao Jingrui Li Peng-Yun Wang Feng Qin Jigang Li Yanglin Ding Yiting Shi Yu Wang Yongqing Yang Yan Guo Jian-Kang Zhu | 2020 | Science China(Life Sciences)2020，63，5： | 94 |
| 2 | PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy显示文摘Mitophagy 是为损坏线粒体的特定的消除的选择 autophagy 的一种重要类型。(PINK1 )导致 PTEN 的通常认为的 kinase 蛋白质 1 催化 ubiquitin (Ub ) 的 phosphorylation 在 PINK1-Parkin-mediated mitophagy 的发作起一个关键作用。(PTEN-L ) 磷酸酶和 tensin 相当或相同的事物(PTEN ) 长是 PTEN 的最新识别的 isoform,与到它的 N 终点的 173 氨基酸的增加。这里,我们报导 PTEN-L 是经由它对 phosphorylated ubiquitin 的蛋白质磷酸酶活动的 mitophagy 的一个新奇否定管理者。我们发现 PTEN-L 在外部 mitochondrial 膜(OMM ) 本地化,而 PTEN-L 的删除支持, PTEN-L 的 overexpression 禁止, mitophagy 由各种各样的损坏线粒体的代理人导致了。机械学地, PTEN-L 能够有效地阻止 Parkin mitochondrial translocation,减少 Parkin phosphorylation,维持它的关上的不活跃的符合构造,并且禁止它的 E3 ligase 活动。更重要地, PTEN-L 在 vivo 减少 phosphorylated ubiquitin (pSer65-Ub ) 的水平,并且在 vitro,磷酸酶试金经由它的蛋白质磷酸酶活动证实那 PTEN-L dephosphorylates pSer65-Ub,独立于它的类脂化合物磷酸酶功能。一起拿,我们的调查结果为 ubiquitin 作为蛋白质磷酸酶表明 PTEN-L 的新奇功能,它抵抗在 mitophagy 正式就职和 mitophagy 的最终的抑制导致前馈控制机制的阻塞的调停 PINK1 的 ubiquitin phosphorylation。因此,理解 PTEN-L 的这新奇功能在控制 mitophagy 的分子的难题提供一关键错过片,在包括象 Parkinsons 那样的 neurodegenerative 混乱的许多重要人的疾病的一个批评过程疾病。 | Liming Wang Yik-Lam Cho Yancheng Tang Jigang Wang Jung-Eun Park Yajun Wu Chunxin Wang Yan Tong Ritu Chawla Jianbin Zhang Yin Shi Shuo Deng Guang Lu Yihua Wu Hayden Weng-Siong Tan Pornteera Pawijit Grace Gui-Yin Lim Hui-Ying Chan Jingzi Zhang Lei Fang Hanry Yu Yih-Cherng Liou Mallilankaraman Karthik Boon-Huat Bay Kah-Leong Lim Siu-Kwan Sze Celestial T. Yap Han-Ming Shen | 2018 | Cell Research2018，28，8： | 25 |
| 3 | Artemisinin, the Magic Drug Discovered from Traditional Chinese Medicine显示文摘Artemisinin and its derivatives represent the most important and influential class of drugs in the fight against malaria. Since the discovery of artemisinin in the early 1970s, the global community has made great strides in characterizing and understanding this remarkable phytochemical and its unique chemical and pharmacological properties. Today, even as artemisinin continues to serve as the foundation for antimalarial therapy, numerous challenges have surfaced in the continued application and development of this family of drugs. These challenges include the emergence of delayed treatment responses to artemisinins in malaria and efforts to apply artemisinins for non-malarial indications. Here, we provide an overview of the story of artemisinin in terms of its past, present, and future. In particular, we comment on the current understanding of the mechanism of action (MOA) of artemisinins, and emphasize the importance of relating mechanistic studies to therapeutic outcomes, both in malarial and non-malarial contexts. | Jigang Wang Chengchao Xu Yin Kwan Wong Yujie Li Fulong Liao Tingliang Jiang Youyou Tu | 2019 | Engineering2019，5，1： | 16 |
| 4 | Traditional Chinese medicine in COVID-19显示文摘COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2(SARSCo V-2)has spread across the globe,posing an enormous threat to public health and safety.Traditional Chinese medicine(TCM),in combination with Western medicine(WM),has made important and lasting contributions in the battle against COVID-19.In this review,updated clinical effects and potential mechanisms of TCM,presented in newly recognized three distinct phases of the disease,are summarized and discussed.By integrating the available clinical and preclinical evidence,the efficacies and underlying mechanisms of TCM on COVID-19,including the highly recommended three Chinese patent medicines and three Chinese medicine formulas,are described in a panorama.We hope that this comprehensive review not only provides a reference for health care professionals and the public to recognize the significant contributions of TCM for COVID-19,but also serves as an evidence-based in-depth summary and analysis to facilitate understanding the true scientific value of TCM. | Ming Lyu Guanwei Fan Guangxu Xiao Taiyi Wang Dong Xu Jie Gao Shaoqin Ge Qingling Li Yuling Ma Han Zhang Jigang Wang Yuanlu Cui Junhua Zhang Yan Zhu Boli Zhang | 2021 | Acta Pharmaceutica Sinica B2021，11，11： | 14 |
| 5 | Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1显示文摘Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis. | Piao Luo Dandan Liu Qian Zhang Fan Yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian Chuanbin Yang Lingyun Dai Han-Ming Shen Jigang Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 14 |
| 6 | Genomic basis for light control of plant development显示文摘Light is one of the key environmental signals regulating plant growth and development.Therefore,understanding the mechanisms by which light controls plant development has long been of great interest to plant biologists.Traditional genetic and molecular approaches have successfully identified key regulatory factors in light signaling,but recent genomic studies have revealed massive reprogramming of plant transcriptomes by light,identified binding sites across the entire genome of several pivotal transcription factors in light signaling,and discovered the involvement of epigenetic regulation in light-regulated gene expression.This review summarizes the key genomic work conducted in the last decade which provides new insights into light control of plant development. | Jigang Li William Terzaghi Xing Wang Deng | 2012 | Protein & Cell2012，3，2： | 11 |
| 7 | Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield显示文摘Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide. | Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen | 2022 | Molecular Plant2022，15，8： | 9 |
| 8 | COP9 signalosome: Discovery, conservation,activity, and function显示文摘The COP9 signalosome(CSN)is a conserved protein complex,typically composed of eight subunits(designated as CSN1 to CSN8)in higher eukaryotes such as plants and animals,but of fewer subunits in some lower eukaryotes such as yeasts.The CSN complex is originally identified in plants from a genetic screen for mutants that mimic light-induced photomorphogenic development when grown in the dark.The CSN complex regulates the activity of cullin-RING ligase(CRL)families of E3 ubiquitin ligase complexes,and play critical roles in regulating gene expression,cell proliferation,and cell cycle.This review aims to summarize the discovery,composition,structure,and function of CSN in the regulation of plant development in response to external(light and temperature)and internal cues(phytohormones). | Nanxun Qin Dongqing Xu Jigang Li Xing Wang Deng | 2020 | Journal of Integrative Plant Biology2020，62，1： | 6 |
| 9 | Experimental validation of dynamic polarization compensation in ground-satellite quantum key distribution显示文摘Ground-satellite quantum key distribution(QKD)is a feasible way to implement global-scale quantum communication.Herein we propose an approach to dynamically compensate the polarization of the photons when passing through the optical telescope used in ground-satellite QKD.Our results experimentally demonstrate that the fidelity of any polarization state after dynamic compensation can be achieved by more than 99.5%,which fulfills the requirements of ground-satellite QKD. | WANG ChaoZe GUO Hui REN JiGang CAO Yuan PENG ChengZhi LIU WeiYue | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，7： | 6 |
| 10 | Impairment of the autophagy-lysosomal pathway in Alzheimer’s diseases: Pathogenic mechanisms and therapeutic potential显示文摘Alzheimer’s disease(AD),the most common neurodegenerative disorder,is characterized by memory loss and cognitive dysfunction.The accumulation of misfolded protein aggregates including amyloid beta(Aβ)peptides and microtubule associated protein tau(MAPT/tau)in neuronal cells are hallmarks of AD.So far,the exact underlying mechanisms for the aetiologies of AD have not been fully understood and the effective treatment for AD is limited.Autophagy is an evolutionarily conserved cellular catabolic process by which damaged cellular organelles and protein aggregates are degraded via lysosomes.Recently,there is accumulating evidence linking the impairment of the autophagy-lysosomal pathway with AD pathogenesis.Interestingly,the enhancement of autophagy to remove protein aggregates has been proposed as a promising therapeutic strategy for AD.Here,we first summarize the recent genetic,pathological and experimental studies regarding the impairment of the autophagy-lysosomal pathway in AD.We then describe the interplay between the autophagy-lysosomal pathway and two pathological proteins,Aβand MAPT/tau,in AD.Finally,we discuss potential therapeutic strategies and small molecules that target the autophagy-lysosomal pathway for AD treatment both in animal models and in clinical trials.Overall,this article highlights the pivotal functions of the autophagy-lysosomal pathway in AD pathogenesis and potential druggable targets in the autophagy-lysosomal pathway for AD treatment. | Wei Zhang Chengchao Xu Jichao Sun Han-Ming Shen Jigang Wang Chuanbin Yang | 2022 | Acta Pharmaceutica Sinica B2022，12，3： | 6 |
| 11 | UV-B-induced photomorphogenesis in Arabidopsis显示文摘Ultraviolet-B(UV-B)is a relatively minor component of sunlight,but can induce stress-related physiological pro-cesses or UV-B-specifi c photomorphogenic responses in plants.In the last decade,signifi cant progress has been made in understanding the UV-B photomorphogenic pathway,including identifi cation of the key components in the pathway,molecular characterization of UV-B pho-toreceptor and perception mechanism,and elucidation of the signal transduction mechanisms from the photo-activated UV-B receptor to downstream gene expression.This review summarizes the key players identifi ed to date in the UV-B photomorphogenic pathway and their roles in mediating UV-B signal transduction. | Jigang Li Li Yang Dan Jin Cynthia D.Nezames William Terzaghi Xing Wang Deng | 2013 | Protein & Cell2013，4，7： | 4 |
| 12 | Arabidopsis small ubiquitin-related modifier protease ASP1 positively regulates abscisic acid signaling during early seedling development~~显示文摘The small ubiquitin-related modifier(SUMO)modification plays an important role in the regulation of abscisic acid(ABA)signaling,but the function of the SUMO protease,in ABA signaling,remains largely unknown.Here,we show that the SUMO protease,ASP1 positively regulates ABA signaling.Mutations in ASP1 resulted in an ABA-insensitive phenotype,during early seedling development.Wild-type ASP1 successfully rescued,whereas an ASP1 mutant(C577S),defective in SUMO protease activity,failed to rescue,the ABA-insensitive phenotype of asp1-1.Expression of ABI5 and MYB30 target genes was attenuated in asp1-1 and our genetic analyses revealed that ASP1 may function upstream of ABI5 and MYB30.Interestingly,ASP1 accumulated upon ABA treatment,and ABA-induced accumulation of ABI5(a positive regulator of ABA signaling)was abolished,whereas ABA-induced accumulation of MYB30(a negative regulator of ABA signaling)was increased in asp1-1.These findings support the hypothesis that increased levels of ASP1,upon ABA treatment,tilt the balance between ABI5 and MYB30towards ABI5-mediated ABA signaling. | Qiongli wang Gao-Ping Qu Xiangxiong Kong Yan Yan Jigang Li Jing Bo Jin | 2018 | Journal of Integrative Plant Biology2018，60，10： | 4 |
| 13 | A new method for recovering paleoporosity of sandstone:case study of middle Es3 member of Paleogene formation in Niuzhuang Sag, Dongying Depression, Bohai Bay Basin in China显示文摘这份报纸为恢复沙岩水库的 paleoporosity 论述一个新方法,份量上定义孔的进化过程。这个方法基于礼品是过去的关键的原则。我们在 Niuzhuang 带中间的 Es3 成员下垂, Dongying 消沉,和 Bohai 海湾盆作为一个例子。在这研究使用的方法认为象建设性的成岩作用和破坏成岩作用的一个限制条件,和影响的现在的孔把孔进化过程划分成二个独立过程,也就是,孔增加和孔减少。沙岩孔的一个进化模型能被联合毛孔增加和毛孔减少效果建立。我们的学习表明孔减少模型是埋葬深度和埋葬时间的连续功能,而孔增加模型主要为 70 的 paleotemperature | Mingjie LIU Zhen LIU Biao WANG Xiaoming SUN Jigang GUO | 2015 | Frontiers of Earth Science2015，9，3： | 4 |
| 14 | PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness显示文摘PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and cryptochromes.It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and development.In this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness.We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter.Consistently,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.Moreover,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by ABA.Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 activation.Taken together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development. | Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang Xiaoji Wang Xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li | 2020 | Molecular Plant2020，13，3： | 3 |
| 15 | HLS bunch current measurement system显示文摘Bunch current is an important parameter for studying the injection fill-pattern in the storage ring and the instability threshold of the bunch, and the bunch current monitor also is an indispensable tool for the top-up injection. A bunch current measurement (BCM) system has been developed to meet the needs of the upgrade project of Hefei Light Source (HLS). This paper presents the layout of the BCM system. The system based on a high-speed digital oscilloscope can be used to measure the bunch current and synchronous phase shift. To obtain the absolute value of bunch-by-bunch current, the calibration coefficient is measured and analyzed. Error analysis shows that the RMS of bunch current is less than 0.01 mA when bunch current is about 5 mA, which can meet project requirement. | YANG YongLiang MA TianJi SUN BaoGen LU Ping WANG JiGang ZOU JunYing TANG LeiLei | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 3 |
| 16 | 18beta-glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs显示文摘Hepatic stellate cells(HSCs)are essential drivers of fibrogenesis.Inducing activated-HSC apoptosis is a promising strategy for treating hepatic fibrosis.18beta-glycyrrhetinic acid(18b-GA)is a natural compound that exists widely in herbal medicines,such as Glycyrrhiza uralensis Fisch,which is used for treating multiple liver diseases,especially in Asia.In the present study,we demonstrated that 18b-GA decreased hepatic fibrosis by inducing the apoptosis in activated HSCs.18b-GA inhibited the expression of a-smooth muscle actin and collagen type Ⅰ alpha-1.Using a chemoproteomic approach derived from activity-based protein profiling,together with cellular thermal shift assay and surface plasmon resonance,we found that 18b-GA covalently targeted peroxiredoxin 1(PRDX1)and peroxiredoxin 2(PRDX2)proteins via binding to active cysteine residues and thereby inhibited their enzymatic activities.18b-GA induced the elevation of reactive oxygen species(ROS),resulting in the apoptosis of activated HSCs.PRDX1 knockdown also led to ROS-mediated apoptosis in activated HSCs.Collectively,our findings revealed the target proteins and molecular mechanisms of 18b-GA in ameliorating hepatic fibrosis,highlighting the future development of 18b-GA as a novel therapeutic drug for hepatic fibrosis. | Qian Zhang Piao Luo Liuhai Zheng Jiayun Chen Junzhe Zhang Huan Tang Dandan Liu Xueling He Qiaoli Shi Liwei Gu Jiahao Li Qiuyan Guo Chuanbin Yang Yin Kwan Wong Fei Xia Jigang Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 3 |
| 17 | Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens显示文摘 | Jigang Han Lei Sun Xiuzhu Dong Zhengqiu Cai Xiaolu Sun Hailian Yang Yunshan Wang Wei Song | 2004 | Systematic and Applied Microbiology2004，，1： | 2 |
| 18 | Distribution of dissolved oxygen and causes of maximum concentration in the Bering Sea in July 2010显示文摘According to data obtained in the Bering Sea during the 4th Chinese National Arctic Research Expedition, the distribution of dissolved oxygen(DO) was studied, causes of its maximum concentration were discussed, and the relationships between DO and other parameters, such as salinity, temperature, and chlorophyll a were analyzed. The results showed DO concentration ranged from 0.53 to 12.05 mg/L in the Bering Sea basin. The upper waters contained high concentrations and the maximum occurred at the depth range from 20 to 50 m. The DO concentration decreased rapidly when the depth was deeper than 200 m and reached the minimum at the depth range from 500 to 1 000 m, and then increased slowly with the depth increasing but still kept at a low level. On the shelf, the DO concentration ranged from 6.53 to 16.63 mg/L with a mean value of 10.75 mg/L, and showed a characteristic of decreasing from north to south. The DO concentration was higher in the area between the Bering Sea and Lawrence Island and was lower in the southeast and southwest of Lawrence Island at the latitude of 62°N. The formation of maximum DO concentration was concerned with phytoplankton photosynthesis and formation of the themocline. To the south of Sta. B07 in the Bering Sea basin, the oxygen produced by photosynthesis permeated to the deeper water and the themocline made it difficult to exchange vertically, and to the north of Sta. B07, the maximum DO concentration occurred above the themocline due to phytoplankton activities. On the shelf, the oxygen produced by phytoplankton photosynthesis gathered at the bottom of the thermocline and formed the DO maximum concentration. In the Bering Sea basin, the DO and salinity showed a weak negative correlation(r=0.40) when the salinity was lower than 33.1, a significant negative correlation(r=0.92) when the salinity ranged from 33.1 to 33.7, and an irregular reversed parabola(r=0.95) when the salinity was greater than 33.7. | SUN Xiuwu LIN Cai CHEN Yong ZHANG Yuanbiao WANG Jigang JI Weidong | 2014 | Acta Oceanologica Sinica2014，33，6： | 2 |
| 19 | Target identification of natural medicine with chemical proteomics approach:probe synthesis,target fishing and protein identification显示文摘Natural products are an important source of new drugs for the treatment of various diseases.However,developing natural productbased new medicines through random moiety modification is a lengthy and costly process,due in part to the difficulties associated with comprehensively understanding the mechanism of action and the side effects.Identifying the protein targets of natural products is an effective strategy,but most medicines interact with multiple protein targets,which complicate this process.In recent years,an increasing number of researchers have begun to screen the target proteins of natural products with chemical proteomics approaches,which can provide a more comprehensive array of the protein targets of active small molecules in an unbiased manner.Typically,chemical proteomics experiments for target identification consist of two key steps:(1)chemical probe design and synthesis and(2)target fishing and identification.In recent decades,five different types of chemical proteomic probes and their respective target fishing methods have been developed to screen targets of molecules with different structures,and a variety of protein identification approaches have been invented.Presently,we will classify these chemical proteomics approaches,the application scopes and characteristics of the different types of chemical probes,the different protein identification methods,and the advantages and disadvantages of these strategies. | Xiao Chen Yutong Wang Nan Ma Jing Tian Yurou Shao Bo Zhu Yin Kwan Wong Zhen Liang Chang Zou Jigang Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 20 | Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy显示文摘Mitochondria as a signaling platform play crucial roles in deciding cell fate.Many classic anticancer agents are known to trigger cell death through induction of mitochondrial damage.Mitophagy,one selective autophagy,is the key mitochondrial quality control that effectively removes damaged mitochondria.However,the precise roles of mitophagy in tumorigenesis and anticancer agent treatment remain largely unclear.Here,we examined the functional implication of mitophagy in the anticancer properties of magnolol,a natural product isolated from herbal Magnolia officinalis.First,we found that magnolol induces mitochondrial depolarization,causes excessive mitochondrial fragmentation,and increases mitochondrial reactive oxygen species(mtROS).Second,magnolol induces PTEN-induced putative kinase protein 1(PINK1)-Parkin-mediated mitophagy through regulating two positive feedforward amplification loops.Third,magnolol triggers cancer cell death and inhibits neuroblastoma tumor growth via the intrinsic apoptosis pathway.Moreover,magnolol prolongs the survival time of tumor-bearing mice.Finally,inhibition of mitophagy by PINK1/Parkin knockdown or using inhibitors targeting different autophagy/mitophagy stages significantly promotes magnolol-induced cell death and enhances magnolol's anticancer efficacy,both in vitro and in vivo.Altogether,our study demonstrates that magnolol can induce autophagy/mitophagy and apoptosis,whereas blockage of autophagy/mitophagy remarkably enhances the anticancer efficacy of magnolol,suggesting that targeting mitophagy may be a promising strategy to overcome chemoresistance and improve anticancer therapy. | Yancheng Tang Liming Wang Tao Yi Jun Xu Jigang Wang Jiang-Jiang Qin Qilei Chen Ka-Man Yip Yihang Pan Peng Hong Yingying Lu Han-Ming Shen Hu-Biao Chen | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 2 |
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