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您的检索式：作者名="Chunlong WEN"
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| 1 | Human 8-cell embryos enable efficient induction of disease-preventive mutations without off-target effect by cytosine base editor显示文摘Approximately 140 million people worldwide are homozygous carriers of APOE4(ε4),a strong genetic risk factor for late onset familial and sporadic Alzheimer’s disease(AD),91%of whom will develop AD at earlier age than heterozygous carriers and noncarriers.Susceptibility to AD could be reduced by targeted editing of APOE4,but a technical basis for controlling the off-target effects of base editors is necessary to develop low-risk personalized gene therapies.Here,we first screened eight cytosine base editor variants at four injection stages(from 1-to 8-cell stage),and found that FNLS-YE1 variant in 8-cell embryos achieved the comparable base conversion rate(up to 100%)with the lowest bystander effects.In particular,80%of AD-susceptibleε4 allele copies were converted to the AD-neutralε3 allele in humanε4-carrying embryos.Stringent control measures combined with targeted deep sequencing,whole genome sequencing,and RNA sequencing showed no DNA or RNA off-target events in FNLS-YE1-treated human embryos or their derived stem cells.Furthermore,base editing with FNLS-YE1 showed no effects on embryo development to the blastocyst stage.Finally,we also demonstrated FNLS-YE1 could introduce known protective variants in human embryos to potentially reduce human susceptivity to systemic lupus erythematosus and familial hypercholesterolemia.Our study therefore suggests that base editing with FNLS-YE1 can efficiently and safely introduce known preventive variants in 8-cell human embryos,a potential approach for reducing human susceptibility to AD or other genetic diseases. | Yinghui Wei Meiling Zhang Jing Hu Yingsi Zhou Mingxing Xue Jianhang Yin Yuanhua Liu Hu Feng Ling Zhou Zhifang Li Dongshuang Wang Zhiguo Zhang Yin Zhou Hongbin Liu Ning Yao Erwei Zuo Jiazhi Hu Yanzhi Du Wen Li Chunlong Xu Hui Yang | 2023 | Protein & Cell2023，14，6： | 0 |
| 2 | Variation of the coastal upwelling off South Java and their impact on local fishery resources显示文摘There is a vast upwelling area induced by the southeast monsoon in the waters off South Java,making the region an important fishing ground.Climate events can affect the variation of upwelling,but oceanographers have different understandings on the extent to which climate events control upwelling in this area,which leads to a lack of basis for studies on the evaluation and mechanisms of the variability of fishery resources in the region.The correlation between environmental parameters,including surface temperature(SST),chlorophyll-a(Chl-a)concentration,and climate event indices in South Java from 2003 to 2020 was analyzed.Results show that the Indian Ocean Dipole(IOD)has a greater influence on the interannual variability of upwelling intensity than ENSO.During the IOD,variations in equatorial latitudinal winds excite different types of Kelvin waves that anomalously deepen or shallow the thermocline,which is the main cause of anomalous variations in upwelling,independent of variations in the local wind field.A correlation between the interannual variability in upwelling and the annual catches was revealed,showing that climatic events indirectly affect fishery resources through upwelling effects.During positive IOD/El Niño periods,strong upwelling delivers more nutrients to the surface layer,which favors fish growth and reproduction,resulting in higher annual catches.A negative IOD/La Niña,on the other hand,leads to weaker upwelling and fewer nutrients into the surface waters.Fish tend to move in deeper waters,making traditional fishing methods less efficient and consequently lower annual catches. | Chunlong WEN Zhenyan WANG Jing WANG Hongchun LI Xingyu SHI Wei GAO Haijun HUANG | 2023 | Journal of Oceanology and Limnology2023，41，4： | 0 |
| 3 | Design and Implementation of Lightning Analysis Software Based on Lightning Location System Data显示文摘This paper discusses the steps about how to design and implement software based on lightning location system data and its localization map.The system contains four major modules such as real-time lightning monitoring,historical query,lightning intensity zoning,lightning disaster statistics,etc.The system is able to automatically generate real-time lightning monitoring image,lightning animation,and lightning intensity zoning image,which contributes to the high accuracy of the calculation results and the efficiency of data analysis.The paper also points out the deficiencies of software life cycle management and proposes actionable solutions. | Rong FAN Chunlong ZHANG Wen'an XIAO Yaoling ZHI | 2020 | Meteorological and Environmental Research2020，11，1： | 0 |
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