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| 1 | Lymphocyte subsets in alcoholic liver disease显示文摘AIM:To compare lymphocyte subsets between healthy controls and alcoholics with liver disease.METHODS:The patient cohort for this study included individuals who were suspected to have alcoholic liver disease(ALD) and who had undergone liver biopsy(for disease grading and staging,doubts about diagnosis,or concurrent liver disease;n = 56).Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones(n = 27).Formalin-fixed,paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls' Prussian blue.The non-alcoholic steatohepatitis score was used to assess markers of ALD.Lymphocyte population subsets were determined by flow cytometry.T lymphocytes were identified(CD3+),and then further subdivided into CD4+ or CD8+ populations.B lymphocytes(CD19+) and natural killer(NK) cell numbers were also measured.In addition to assessing lymphocyte subpopulation differences between ALD patients and controls,we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal controls.RESULTS:The patient cohort primarily consisted of older men.Active alcoholism was present in 66.1%.Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 kg.Cirrhosis was present in 39.3% of the patients and 66.1% had significant fibrosis(perisinusoidal and portal/periportal fibrosis,bridging fibrosis,or cirrhosis) in their liver samples.The average Mayo end-stage liver disease score was 7.6.No hereditary hemochromatosis genotypes were found.ALD patients(n = 56) presented with significant lymphopenia(1.5 × 109/L ± 0.5 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001),due to a decrease in all lymphocyte subpopulations,except for NK lymphocytes:CD3+(1013.0 ± 406.2/mm3 vs 1523.0 ± 364.6/mm3,P < 0.0001),CD4+(713.5 ± 284.7/mm3 vs 992.4 ± 274.7/mm3,P < 0.0001),CD8+(262.3 ± 140.4/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(120.6 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).CD8+ lymphocytes suffered the greatest reduction,as evidenced by an increase in the CD4+/CD8+ ratio(3.1 ± 1.3 vs 2.3 ± 0.9,P = 0.013).This ratio was associated with the stage of fibrosis on liver biopsy(rs = 0.342,P = 0.01) and with Child-Pugh score(rs = 0.482,P = 0.02).The number of CD8+ lymphocytes also had a positive association with serum ferritin levels(rs = 0.345,P = 0.009).Considering only patients with active alcoholism but not cirrhosis(n = 27),we found similar reductions in total lymphocyte counts(1.8 × 109/L ± 0.3 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P = 0.018),and in populations of CD3+(1164.7 ± 376.6/mm3 vs 1523.0 ± 364.6/mm3,P = 0.001),CD4+(759.8 ± 265.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.003),CD8+(330.9 ± 156.3/mm3 vs 478.9 ± 164.6/mm3,P = 0.002),and CD19+(108.8 ± 64.2/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).In these patients,the CD4+/CD8+ ratio and the number of NK lymphocytes was not significantly different,compared to controls.Comparing patients with liver cirrhosis but without active alcohol consumption(n = 11),we also found significant lymphopenia(1.3 × 109/L ± 0.6 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001) and decreases in populations of CD3+(945.5 ± 547.4/mm3 vs 1523.0 ± 364.6/mm3,P = 0.003),CD4+(745.2 ± 389.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.032),CD8+(233.9 ± 120.0/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(150.8 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P = 0.001).The NK lymphocyte count was not significantly different,but,in this group,there was a significant increase in the CD4+/CD8+ ratio(3.5 ± 1.3 vs 2.3 ± 0.9,P = 0.01).CONCLUSION:All patient subsets presented with decreased lymphocyte counts,but only patients with advanced fibrosis presented with a significant increase in the CD4+/CD8+ ratio. | Luís Costa Matos Paulo Batista Nuno Monteiro Joo Ribeiro Maria A Cipriano Pedro Henriques Fernando Giro Armando Carvalho | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | In vitro Ruminal Gas Production Kinetics of Four Fodder Trees Ensiled With or Without Molasses and Urea显示文摘This study investigated if the addition of urea (U), molasses (M) or their 1:1 (v/v) mixture during ensiling increases the nutritional value of forage from four fodder trees (Prunus persica, Leucaena esculenta, Acacia farnesiana, and Prunus domestica). Forage samples of fodder trees were collected in triplicate (three individual samples of each species) and subjected to an in vitro gas production (GP) procedure. Fermentation at 24 h (GP 24), short-chain volatile fatty acids (SCFA), and microbial crude protein production (MCP), in vitro organic matter digestibility (OMD), metabolizable energy (ME) and dry matter degradability (DMD) were estimated. Forage samples were incubated for 72 h in an incubator at 39oC and the volume of GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48, and 72 h of incubation using the reading pressure technique. The rumen fermentation profiles were highest for P. persica, which showed the highest (P<0.0001) DMD, ME, OMD, SCFA, GP 24 and MCP. On the other hand L. esculenta had the lowest (P<0.0001) DMD, SCFA, MCP; P. domestica had the lowest (P<0.0001) OMD. The addition of M to silage increased (P<0.0001) ME and OMD, as well as GP. However, the addition of U and the mixture of U and M reduced (P<0.0001) DMD, ME, OMD, SCFA, GY 24 and MCP. These results show that P. persica has the highest nutritive value and L. esculenta the lowest for ruminants. Additionally, the addition of M to forage from fodder trees increases rumen GP and fermentation, which may improve nutrient utilization in ruminants. | Abdelfattah Z M Salem ZHOU Chuan-she TAN Zhi-liang Miguel Mellado Moises Cipriano Salazar Mona M M Y Elghandopur Nicholas E Odongo | 2013 | Journal of Integrative Agriculture2013，12，7： | 3 |
| 3 | ARFI elastography in patients with chronic autoimmune liver diseases: A preliminary study显示文摘 | S. Righi E. Fiorini C. De Molo V. Cipriano F. Cassani L. Muratori M. Lenzi A.M. Morselli Labate C. Serra | 2012 | Journal of Ultrasound2012，，4： | 2 |
| 4 | Preliminary results of antiscarring therapy in the prevention of postendoscopic esophageal mucosectomy strictures显示文摘 | Yuhsin Wu Steve J. Schomisch Cassandra Cipriano Amitabh Chak Richard H. Lash Jeffrey L. Ponsky Jeffrey M. Marks | 2014 | Surgical Endoscopy2014，，2： | 2 |
| 5 | Multi-Centre European Experience with Intragastric Balloon in Overweight Populations: 13 Years of Experience显示文摘 | Alfredo Genco Gontrand López-Nava Christian Wahlen Roberta Maselli Massimiliano Cipriano Maria Mara Arenas Sanchez Chantal Jacobs Michele Lorenzo | 2013 | Obesity Surgery2013，，4： | 2 |
| 6 | Triple Therapy with High‐Dose Proton‐Pump Inhibitor, Amoxicillin, and Doxycycline Is Useless for H elicobacter pylori Eradication: A Proof‐of‐Concept Study显示文摘 | Nuno Almeida José M. Rom?ozinho Maria M. Donato Cristina Luxo Olga Cardoso Maria A. Cipriano Carol Marinho Carlos Sofia | 2014 | Helicobacter2014，，2： | 1 |
| 7 | Replacement of histidine 340 with alanine inactivates the group A Streptococcus extracellular cysteine protease virulence factor 显示文摘 | Gubba S Cipriano V Musser JM | 2000 | Infect Immun2000，68，6： | 1 |
| 8 | Dilute betadine lavage before closure for the prevention of acute postoperative deep periprosthetic joint infection显示文摘 | Brown NM Cipriano CA Moric M | 2012 | J Arthroplasty2012，27，1： | 1 |
| 9 | Isolation of infective and non-infective Eperythrozoon suis bodies from the whole blood of infected swine显示文摘 | Hall S M Cipriano J A Schoneweis D A | 1988 | Vet Rec1988，123，： | 1 |
| 10 | NK and NKT cells in aging and longevity:role of zinc and metallothioneins显示文摘 | Mocchegiani E Giacconi R Cipriano C | | 0，，04： | 1 |
| 11 | External validation of a classification for methylene blue magnification chromoendoscopy in premalignant gastric lesions显示文摘 | Miguel Areia Pedro Amaro Mário Dinis-Ribeiro Maria Augusta Cipriano Carol Marinho Altamiro Costa-Pereira Carlos Lopes Luís Moreira-Dias José Manuel Rom?ozinho Hermano Gouveia Diniz Freitas Maximino Correia Leit?o | 2008 | Gastrointestinal Endoscopy2008，，7： | 1 |
| 12 | Physiological effects of tapering in highly trained athletes显示文摘 | SHEPLEY B MACDOUGALL J D CIPRIANO N | 1992 | J Appl Physiol1992，72，： | 1 |
| 13 | Evaluation of methods for the quantification of experimental myocardial infarction显示文摘 | Roberts AJ Cipriano PR Alonso DR Jacbostein JG Combes JR Gay WA Jr | 1978 | Circulation1978，57，1： | 1 |
| 14 | hnmunization of brook trout, Salvelinusfontinalis against Aeromonas salmonicida: immunogenicity of virulent isolates and protective of different antigen 显示文摘 | Cipriano R | 1982 | Can J Fish Aquat Sci1982，39，： | 1 |
| 15 | Lung cancer treatment casts, including patient responsibility, by disease stage and treatment modality, 1992 to 2003显示文摘 | Cipriano LE Romanus D Earle CC | 2011 | Value Health2011，14，1： | 1 |
| 16 | Once-weekly transdermal buprenorphine application results in sustained and consistent steady-state plasma levels显示文摘 | KAPIL RP CIPRIANO A FRIEDMAN K | 2013 | J Pain Symptom Manage2013，46，1： | 1 |
| 17 | The importance of health information technology in care coordination and transitional care显示文摘 | Cipriano PF Bowles K Dailey M | 2013 | Nurs Outlook2013，61，6： | 1 |
| 18 | N2 Fixation by unicellular bacterioplankton from the Atlanticand Pacifie Oceans: Phylogeny and in situ rates显示文摘 | Falc n L I E J Carpenter F Cipriano | 2004 | Appl Environ Microbiol2004，312，： | 1 |
| 19 | Isolation of infective and non-infective Eperythrozoon suis bodies from the whole blood of infected swine显示文摘 | Hall S M Cipriano J A Schoneweis D A | 1988 | Vet Rec1988，123，： | 1 |
| 20 | Isolation of infective and non-infective Eperythrozoon suis bodies from the whole blood of infected swine显示文摘 | Hall SM Cipriano JA Schoneweis DA et aJ | 1988 | Vet Rec1988，123，： | 1 |
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