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您的检索式：作者名="Congjiao He"
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| 1 | Multi-parameters uncertainty analysis of logistic support process based on GERT显示文摘The uncertainty analysis is an effective sensitivity analysis method for system model analysis and optimization. However,the existing single-factor uncertainty analysis methods are not well used in the logistic support systems with multiple decision-making factors. The multiple transfer parameters graphical evaluation and review technique(MTP-GERT) is used to model the logistic support process in consideration of two important factors, support activity time and support activity resources, which are two primary causes for the logistic support process uncertainty. On this basis,a global sensitivity analysis(GSA) method based on covariance is designed to analyze the logistic support process uncertainty. The aircraft support process is selected as a case application which illustrates the validity of the proposed method to analyze the support process uncertainty, and some feasible recommendations are proposed for aircraft support decision making on carrier. | Yong Wu Xing Pan Rui Kang Congjiao He Liming Gong | 2014 | Journal of Systems Engineering and Electronics2014，25，6： | 5 |
| 2 | miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1显示文摘Background:The egg production performance of chickens is affected by many factors,including genetics,nutrition and environmental conditions.These factors all play a role in egg production by affecting the development of follicles.MicroRNAs(miRNAs)are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after transcription.In the animal reproduction process,miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells(GCs).Results:In this study,we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy chickens.We identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken follicles.The function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p mimics.In the study,we used qPCR,western blot and flow cytometry to detect granulosa cell apoptosis,autophagy and steroid hormone synthesis.Confocal microscopy and transmission electron microscopy are used for the observation of autophagolysosomes.The levels of estradiol(E2),progesterone(P4),malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by ELISA.The results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells,and also contributed in steroid hormones and reactive oxygen species(ROS)production.In addition,the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of miR-30a-5p.The rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory pathway.Conclusions:In summary,after deep miRNA sequencing on healthy and atretic follicles,the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1. | Haorong He Dongmei Li Yongtong Tian Qinyao Wei Felix Kwame Amevor Congjiao Sun Chunlin Yu Chaowu Yang Huarui Du Xiaosong Jiang Menggen Ma Can Cui Zhichao Zhang Kai Tian Yao Zhang Qing Zhu Huadong Yin | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 0 |
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