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| 1 | Genome-wide Identification of microRNAs that Respond to Drought Stress in Seedlings of Tertiary Relict Ammopiptanthus mongolicus显示文摘microRNAs(miR NAs) are important post-transcriptional regulators of gene expression in plant abiotic stress responses. Ammopiptanthus mongolicus is a typical evergreen woody xerophyte, which makes it an ideal model system for studying drought tolerance in woody plants. The response of mi RNAs to drought stress is still unknown in this plant. In this research, we obtained 34 695 665 raw reads from two small RNA libraries constructed from control and drought-treated A. mongolicus seedlings by the Illumina deep sequencing technology. Length analysis revealed that reads of 20, 21, and 24 nucleotides accounted for the majority of the small RNAs in the two libraries. Sequence analyses identified 65 conserved mi RNA sequences from 190 members of 31 mi RNA families and 20 potential novel mi RNAs from 17 families that were differentially expressed between the two libraries. The expression patterns of 25 of these mi RNAs were significantly different in the two libraries, but only am-mi R4 a-c was downregulated during drought stress, the other 24 were upregulated. The expression trends determined by the Hi Seq sequencing and by q RT-PCR were similar. Furthermore, 35 target genes for the conserved mi RNA were markedly changed and 52 target genes for the potential novel mi RNAs were predicted and their functions were annotated by computational analysis. Our results provide new molecular evidence for understanding the molecular mechanisms of drought response and tolerance in A. mongolicus. | LIU Shengli NI Yanjie HE Qian WANG Jinyu CHEN Yuzhen LU Cunfu | 2017 | Horticultural Plant Journal2017，3，5： | 3 |
| 2 | Wireless Strain Measurement Based on a Microstrip Patch Antenna显示文摘Wireless interrogation of a rectangular microstrip patch antenna for strain measurement is investigated by simulations.To analyze the antenna performance,a microstrip line-feeding patch antenna at 10 GHz is designed.A patch antenna wirelessly fed by a horn is proposed to measure the strain.The direction information of strain detected by the patch antenna is also considered.The strain can be detected both in the width and length directions.It is shown that the strain can be measured wirelessly using a standard horn antenna.This kind of wireless strain-sensing technique offers significant potential for wireless structural health monitoring(SHM),especially for high-end equipment. | Song Guorong Sun Tingting Lu Yan Yan Tianting Wang Xuedong He Cunfu Wu Bin | 2017 | Transactions of Nanjing University of Aeronautics and Astronautics2017，34，5： | 2 |
| 3 | Role of mucilage during achene germination and sprout growth of the endangered Tibetan medicinal herb Mirabilis himalaica (Nyctaginaceae) exposed to abiotic stresses显示文摘Aim Mirabilis himalaica(Nyctaginaceae)is an endangered medicinal plant mainly distributed in the plateau region of northern Tibet,China.The outer surface of M.himalaica achenes is covered by a pectinaceous mucilaginous layer upon hydration.However,the role of the achene mucilage is poorly understood.in this study,we inves-tigated the effects of mucilage on achene germination and sprout growth under abiotic stress to explain how M.himalaica survive the alpine environment.Methods We investigated the effect of mucilage on achenes germination by contrast the capacity of water absorption,dehydration and respira-tion of intact achene and the achene with mucilage removal.We performed abiotic stresses experiments including drought stress,salt stress,cold stress and high temperature stress,and quantified the effects of mucilage removal on achene germination rate,root and shoot lengths of seedlings.Important Findings Mucilage is extremely hydrophilic,and the mass of intact achenes can be 9-fold greater than that of demucilaged achenes.The removal of the mucilaginous layer did not significantly change final germination percentages under ideal conditions,but intact achenes(i.e.with muci-lage)took longer to germinate.The mucilage significantly decreased seed respiration rates by acting as a physical barrier that prevented oxygen diffusion.Germination rates,shoot and root growth of intact achenes were higher than those of demucilaged ones during exposures to cold,heat,osmotic and salt stresses.Achene mucilage presumably plays an ecologically important role in the life cycle of M.himalaica by aiding the critical achene germination and early seedling growth in the stressful habitats of the plateau region of northern Tibet. | Yuzhen Chen Lu Zhang Xiao Lu Xiaozhong Lan Man Shen Cunfu Lu | 2018 | Journal of Plant Ecology2018，11，2： | 1 |
| 4 | High Speed Imaging Techniques for Pulse Nuclear Radiation Source显示文摘 | Wang Kuilu Lu Min Liu Cunfu and Kang Dechun | 1988 | High Speed Photography and Photonies1988，1032，： | 1 |
| 5 | Expression of a Carrot Antifreeze Protein Gene in Escherichia coli显示文摘The recombinant expression vector pET43.1b-AFP, which contains full encoding region of a carrot 36 kD antifreeze protein (AFP) gene was constructed. The recombinant was transformed into expression host carrying T7 RNA polymerase gene (DE3 lysogen) and induced by 1 mmol稬-1 IPTG (isopropyl--D-thiogalactoside) to express 110 kD polypeptide of AFP fusion protein. The analysis of product solubility revealed that pET43.1b-AFP was predominately soluble, and the expressed amount reached the maximum after the IPTG treatment for 3 h. | Ma Xinyu Shen Xin Lu Cunfu* College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China | 2003 | Forestry Studies in China2003，5，4： | 0 |
| 6 | Achene mucilage formation process and extrusion from hydrated pericarp of Mirabilis himalaica显示文摘Myxospermy is an important feature of achenes of the alpine plant Mirabilis himalaica,and the achene mucilage increases the germination rate and early seedling growth during exposure to abiotic stresses,which has important functions that allow M.himalaica to survive the extreme climate of the Tibet Plateau.However,achene formation and mucilage extrusion are poorly understood.In the present study,comprehensive analyses were performed on mucilage production during achene development and mucilage release from hydrated achene pericarp in M.himalaica.First,fertilization initiated the development of M.himalaica achenes,during which their color,size and texture were altered dramatically.Second,using a metachromatic staining procedure,cytological events,the establishment of mucilage secretory cells in the inner epicarp layer were observed.The hydration of mature achenes led to the rapid bursting of mucilage secretory cells,which released a hydrophilic gel that surrounded the achenes.Finally,enzymatic digestion indicated that major components of the mucilage were pectins;glucose(41.40%),rhamnose(26.58%),galactose(18.33%),trehalose(12.12%),and mannose(1.57%)were found to be the components of achene by using ion-exchange chromatography. | Qingqing Huang Lu Zhang Xiaozhong Lan Yuzhen Chen Cunfu Lu | 2022 | Horticultural Plant Journal2022，8，2： | 0 |
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