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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms显示文摘这篇文章在开发,基因疾病和癌症考察分子的结构,表达式模式,生理的函数,病理学的角色和 Twist1 的分子的机制。Twist1 是基本 helix-loop-helix 包含域的抄写因素。以便绑 Nde1 电子盒子元素并且激活或镇压形成 homo-dimers 或 hetero-dimers 它的目标基因。在开发期间, Twist1 为中层说明和区别是必要的。人的 Twist1 基因的异质接合的 loss-of-function 变化包括 Saethre-Chotzen 症候群引起几疾病。Twist1 空的老鼠胚胎与打开的头部的神经试管和有缺点的头间充组织死,体节和手足芽。Twist1 在胸被表示,肝,前列腺,胃并且癌症的另外的类型,和它的表示通常与侵略、变形的癌症显型被联系。在癌症房间, Twist1 是由包括 SRC-1 的多重因素的 upregulated, STAT3, MSX2, HIF-1α,连接 integrin 的 kinase 和 NF-κ B。Twist1 显著地提高上皮间充质的转变(EMT ) 和癌症房间移植和侵略,因此支持癌症转移。Twist1 由直接镇压部分地支持 EMT 由招募的 E-cadherin 表示改变的 nucleosome 和为基因压抑并且由 upregulating Bmi1 的 deacetylase 建筑群, AKT2, YB-1,等等。新兴的证据也建议 Twist1 在扩大和癌症干细胞的化学疗法的抵抗起一个作用。Twist1 由支持 Twist1 功能的调节的人的转移和鉴定的机制的进一步的理解可以为开发新策略禁止 EMT 和癌症转移保持诺言。 | Qian Qin Young Xu Tao He Chunlin Qin Jianming Xu | 2012 | Cell Research2012，22，1： | 66 |
| 3 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 4 | A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice显示文摘A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice (Oryza sativa L.) varieties.In this study,we generated an F 2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64),an elite indica small-grain cultivar.Using QTL analysis,17 QTLs for five grain traits were detected on four different chromosomes.Eight of the QTLs were newly-identified in this study.In particular,qGL3-1,a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation,was fine-mapped to the 17 kb region of chromosome 3.A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL.A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10,resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid.This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat.Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect,and is suitable for use in molecular marker-assisted selection. | Zejun Hu Haohua He Shiyong Zhang Fan Sun Xiaoyun Xin Wenxiang Wang Xi Qian Jingshui Yang Xiaojin Luo | 2012 | Journal of Integrative Plant Biology2012，54，12： | 41 |
| 5 | Acupuncture and regulation of gastrointestinal function显示文摘In China, acupuncture has been considered an effective method for treating gastrointestinal(GI) dysfunction diseases for thousands of years. In fact, acupuncture has gained progressive acceptance from both practitioners and patients worldwide. However, the therapeutic effects and underlying mechanisms in treating GI dysfunction have not yet been established due to a lack of systematic and comprehensive review articles. Therefore, the aim of this review is to discuss the efficacy of acupuncture as a treatment for GI dysfunction and the associated underlying mechanisms. A search of Pub Med was conducted for articles that were published over the past 10 years using the terms 'acupuncture', 'gastrointestine', and other relevant keywords. In the following review, we describe the effect and underlying mechanisms of acupuncture on GI function from the perspectives of GI motility, visceral sensitivity, the GI barrier, and the brain-gut axis. The dual regulatory effects of acupuncture may manifest by promoting gastric peristalsis in subjects with low initial gastric motility, and suppressing peristalsis in subjects with active initial motility. In addition, the regulation of acupuncture on gastric motility may be intensitydependent. Our findings suggest that further studies are needed to investigate the effects and more systematic mechanisms in treating GI dysfunction, and to promote the application of acupuncture for the treatment of GI diseases. | Hui Li Tian He Qian Xu Zhe Li Yan Liu Fang Li Bo-Feng Yang Cun-Zhi Liu | 2015 | World Journal of Gastroenterology2015，21，27： | 41 |
| 6 | Trends in Lipids Level and Dyslipidemia among Chinese Adults, 2002-2015显示文摘Objective To investigate the trends of lipid profiles and dyslipidemia among Chinese adults from 2002 to 2015.Methods Data were collected from three nationally representative cross-sectional surveys.Fasting venous blood samples were collected and serum lipids were tested by biochemical analysis and enzymatic determination.Lipid levels and the prevalence of dyslipidemia among adults were analyzed with complex sampling weighting adjustment for age and gender.Results The weighted means of TC, TG, and LDL-c significantly increased linearly from 3.93, 1.12, and 2.12 mmol/L in 2002 to 4.59, 1.41, and 2.78 mmol/L in 2010 and then to 4.63, 1.47, and 2.87 mmol/L in 2015, respectively;by contrast, HDL-c levels decreased significantly from 1.30 mmol/L to 1.26 mmol/L over the same period.Similar trends in mean non-HDL-c and lipid-related ratios were observed.The weighted dyslipidemia prevalence linearly increased;in particular, hypercholesterolemia increased from 1.6% to 5.6% and then to 5.8%, hypertriglyceridemia increased from 5.7% to 13.6% and then to 15.0%, low HDL-c increased from 18.8% to 35.5% and then to 24.9%, and high LDL-c increased from 1.3% to 5.6% and then to 7.2%(P for trend <0.001).Conclusion Dyslipidemia increased among Chinese adults from 2002 to 2015.Development of a comprehensive strategy to decrease lipid levels in this population is urgently required. | SONG Peng Kun MAN Qing Qing LI Hong PANG Shao Jie JIA Shan Shan LI Yu Qian HE Li ZHAO Wen Hua ZHANG Jian | 2019 | Biomedical and Environmental Sciences2019，32，8： | 45 |
| 7 | Probiotics in Helicobacter pylori eradication therapy: A systematic review and meta-analysis显示文摘AIM: To summarize the evidence from randomized controlled trials(RCTs) regarding the effect of probiotics by using a meta-analytic approach.METHODS: In July 2013, we searched PubM ed, EMBASE, Ovid, the Cochrane Library, and three Chinese databases(Chinese Biomedical Literature Database, Chinese Medical Current Content, and Chinese Scientific Journals database) to identify relevant RCTs.We included RCTs investigating the effect of a combination of probiotics and standard therapy(probiotics group) with standard therapy alone(control group).Risk ratios(RRs) were used to measure the effect of probiotics plus standard therapy on Helicobacter pylori(H.pylori) eradication rates, adverse events, and patient compliance using a random-effect model.RESULTS: We included data on 6997 participants from 45 RCTs, the overall eradication rates of the probiotic group and the control group were 82.31% and 72.08%, respectively.We noted that the use of probiotics plus standard therapy was associated with an increased eradication rate by per-protocol set analysis(RR = 1.11; 95%CI: 1.08-1.15; P < 0.001) or intention-totreat analysis(RR = 1.13; 95%CI: 1.10-1.16; P < 0.001).Furthermore, the incidence of adverse events was 21.44% in the probiotics group and 36.27% in the control group, and it was found that the probiotics plus standard therapy significantly reduced the risk of adverse events(RR = 0.59; 95%CI: 0.48-0.71; P < 0.001), which demonstrated a favorable effect of probiotics in reducing adverse events associated with H.pylori eradication therapy.The specific reduction in adverse events ranged from 30% to 59%, and this reduction was statistically significant.Finally, probiotics plus standard therapy had little or no effect on patient compliance(RR = 0.98; 95%CI: 0.68-1.39; P = 0.889).CONCLUSION: The use of probiotics plus standard therapy was associated with an increase in the H.pylori eradication rate, and a reduction in adverse events resulting from treatment in the general population.However, this therapy did not improve patient compliance. | Min-Min Zhang Wei Qian Ying-Yi Qin Jia He Yu-Hao Zhou | 2015 | World Journal of Gastroenterology2015，21，14： | 41 |
| 8 | Prevalence, risk factors, clinical course, and outcome of acute kidney injury in Chinese intensive care units: a prospective cohort study显示文摘 | WEN Ying JIANG Li XU Yuan QIAN Chuan-yun LI Shu-sheng QIN Tie-he CHEN Er-zhen LIN Jian-dong AI Yu- hang WU Da-wei WANG Yu-shan SUN Ren-hua HU Zhen-jie CAO Xiang-yuan ZHOU Fa-chun HE Zhen-yang ZHOU Li-hua AN You-zhong KANG Yan MA Xiao-chun YU Xiang-you ZHAO Ming-yan XI Xiu-ming DU Bin | 2013 | Chinese Medical Journal2013，，23： | 40 |
| 9 | Review of risk factors for human echinococcosis prevalence on the Qinghai-Tibet Plateau, China: a prospective for control options显示文摘Objective: Echinococcosis is a major parasitic zoonosis of public health importance in western China. In 2004, the Chinese Ministry of Health estimated that 380,000 people had the disease in the region. The Qinghai-Tibet Plateau is highly co-endemic with both alveolar echinococcosis (AE) and cystic echinococcosis (CE). In the past years, the Chinese government has been increasing the financial support to control the diseases in this region. Therefore, it is very important to identify the significant risk factors of the diseases by reviewing studies done in the region in the past decade to help policymakers design appropriate control strategies. Review: Selection criteria for which literature to review were firstly defined. Medline, CNKI (China National Knowledge Infrastructure), and Google Scholar were systematically searched for literature published between January 2000 and July 2011. Significant risk factors found by single factor and/or multiple factors analysis were listed, counted, and summarized. Literature was examined to check the comparability of the data;age and sex specific prevalence with same data structures were merged and used for further analysis. A variety of assumed social, economical, behavioral, and ecological risk factors were studied on the Plateau. Those most at risk were Tibetan herdsmen, the old and female in particular. By analyzing merged comparable data, it was found that females had a significant higher prevalence, and a positive linearity relationship existed between echinococcosis prevalence and increasing age. In terms of behavioral risk factors, playing with dogs was mostly correlated with CE and/or AE prevalence. In terms of hygiene, employing ground water as the drinking water source was significantly correlated with CE and AE prevalence. For definitive hosts, dog related factors were most frequently identified with prevalence of CE or/and AE;fox was a potential risk factor for AE prevalence only. Overgrazing and deforestation were significant for AE prevalence only. Conclusion: Tibetan herdsmen communities were at the highest risk of echinococcosis prevalence and should be the focus of echinococcosis control. Deworming both owned and stray dogs should be a major measure for controlling echinococcosis;treatment of wild definitive hosts should also be considered for AE endemic areas. Health education activities should be in concert with the local people's education backgrounds and languages in order to be able to improve behaviors. Further researches are needed to clarify the importance of wild hosts for AE/CE prevalence, the extent and range of the impacts of ecologic changes (overgrazing and deforestation) on the AE prevalence, and risk factors in Tibet. | Qian Wang Yan Huang Liang Huang Wenjie Yu Wei He Bo Zhong Wei Li Xiangman Zeng Dominique A Vuitton Patrick Giraudoux Philip S Craig Weiping Wu | 2014 | Infectious Diseases of Poverty2014，3，1： | 35 |
| 10 | Protective effects of emodin and astragalus polysaccharides on chronic hepatic injury in rats显示文摘背景中国药在 hepatoprotective 治疗起一个重要作用。这研究被进行在长期的肝的 injury.Methods 的一个老鼠模型调查 emodin 和距骨多糖( APS )的保护的效果长期的肝的损害被一个星期包含40%碳四氯化物( CCI4 )两次的一个橄榄油答案的皮下注射的注射导致,除了在为 12 个星期的喝的水广告 libitum 的79.5% maizena ,20%脂肪,0.5%胆固醇,和10%酒精的一本食谱。同时,老鼠暴露于 emodin 的不同集中(40 mg ????????????????????? 洠汥楬畴 ??????????????瀠敲業浵刮獥汵獴 吗?? | DANG Shuang-suo ZHANG Xin JIA Xiao-li CHENG Yan-an SONG Ping LIU En-qi HE Qian LI Zong-fang | 2008 | Chinese Medical Journal2008，，11： | 34 |
| 11 | Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors显示文摘在 enucleated 鸡蛋的体的房间的 Reprogramming 做了多丽,绵羊,在 1996 的第一个成功地克隆的哺乳动物。然而,绵羊的机制体的房间 reprogramming 还没被探讨了。而且,绵羊胚胎的茎(ES ) 细胞仍然不是可得到的,它限制精确修改基因的绵羊的产生。在这研究,我们报导体的房间能是的那只绵羊直接 reprogrammed 到导致的 pluripotent 用定义因素(Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 大 T 和 hTERT ) 的茎(iPS ) 房间。我们的观察显示从绵羊的体的细胞比从另外的种,在 iPS,细胞被报导了的体的细胞对改编更困难。我们证明绵羊 iPS 房间表示 ES 房间标记,包括碱的磷酸酶, Oct4, Nanog, Sox2, Rex1,阶段特定的胚胎的 antigen-1, TRA-1-60, TRA-1-81 和 E-cadherin。绵羊 iPS 房间展出了正常 karyotypes 并且能在 vitro 并且在 teratomas 区分进所有三细菌层。我们的学习可以帮助在绵羊揭示体的房间 reprogramming 的机制并且提供一个平台为绵羊 ES 房间探索文化条件。而且,绵羊 iPS 房间可以直接被用来产生精确修改基因的绵羊。 | Lei Bao Lixiazi He Jijun Chen Zhao Wu Jing Liao Lmgdun Rao Jiangtao Ren Hui Li Hui Zhu Lei Qian Yijun Gu Huimin Dai Xun Xu Jinqiu Zhou Wen Wang Chun Cui Lei Xiao | 2011 | Cell Research2011，21，4： | 32 |
| 12 | Overexpression of the maize GRF10, an endogenous truncated growth-regulating factor protein, leads to reduction in leaf size and plant height显示文摘It has long been thought that growth‐regulating factors(GRFs) gene family members act as transcriptional activators to play important roles in multiple plant developmental processes. However, the recent characterization of Arabidopsis GRF7 showed that it functions as a transcriptional repressor of osmotic stress‐responsive genes. This highlights the complex and diverse mechanisms by which different GRF members use to take action. In this study, the maize(Zea mays L.) GRF10 was functionally characterized to improve this concept. The deduced ZmGRF10 protein retains the N‐terminal QLQ and WRC domains, the characteristic regions as protein‐interacting and DNA‐binding domains, respectively. However,it lacks nearly the entire C‐terminal domain, the regions executing transactivation activity. Consistently, ZmGRF10 protein maintains the ability to interact with GRF‐interacting factors(GIFs) proteins, but lacks transactivation activity.Overexpression of ZmGRF10 in maize led to a reduction in leaf size and plant height through decreasing cell proliferation,whereas the yield‐related traits were not affected. Transcriptome analysis revealed that multiple biological pathways were affected by ZmGRF10 overexpression, including a few transcriptional regulatory genes, which have been demonstrated to have important roles in controlling plant growth and development. We propose that ZmGRF10 aids in fine‐tuning the homeostasis of the GRF‐GIF complex in the regulation of cell proliferation. | Lei Wu Dengfeng Zhang Ming Xue Jianjun Qian Yan He Shoucai Wang | 2014 | Journal of Integrative Plant Biology2014，56，11： | 31 |
| 13 | Fhe roles and functional mechanisms of interleukin-17 amily cytokines in mucosal immunity显示文摘 | Xinyang Song Xiao He Xiaoxia Li Youcun Qian | 2016 | Cellular & Molecular Immunology2016，13，4： | 29 |
| 14 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 15 | Ammonia volatilization from a Chinese cabbage field under different nitrogen treatments in the Taihu Lake Basin, China显示文摘Ammonia(NH_3) volatilization is a major pathway of nitrogen(N) loss from soil-crop systems.As vegetable cultivation is one of the most important agricultural land uses worldwide,a deeper understanding of NH_3 volatilization is necessary in vegetable production systems.We therefore conducted a 3-year(2010–2012) field experiment to characterize NH_3 volatilization and evaluate the effect of different N fertilizer treatments on this process during the growth period of Chinese cabbage.Ammonia volatilization rate,rainfall,soil water content,p H,and soil NH_4^+were measured during the growth period.The results showed that NH_3 volatilization was significantly and positively correlated to topsoil p H and NH4+concentration.Climate factors and fertilization method also significantly affected NH_3 volatilization.Specifically,organic fertilizer(OF) increased NH_3 volatilization by 11.77%–18.46%,compared to conventional fertilizer(CF,urea),while organic–inorganic compound fertilizer(OIF) reduced NH_3 volatilization by 8.82%–12.67% compared to CF.Furthermore,slow-release fertilizers had significantly positive effects on controlling NH_3 volatilization,with a 60.73%–68.80% reduction for sulfur-coated urea(SCU),a 71.85%–78.97% reduction for biological Carbon Power~® urea(BCU),and a 77.66%–83.12% reduction for bulk-blend controlled-release fertilizer(BBCRF)relative to CF.This study provides much needed baseline information,which will help in fertilizer choice and management practices to reduce NH_3 volatilization and encourage the development of new strategies for vegetable planting. | Linan Shan Yunfeng He Jie Chen Qian Huang Hongcai Wang | 2015 | Journal of Environmental Sciences2015，27，12： | 28 |
| 16 | Rhizospheric microbial communities are driven by Panax ginseng at different growth stages and biocontrol bacteria alleviates replanting mortality显示文摘The cultivation of Panax plants is hindered by replanting problems, which may be caused by plantdriven changes in the soil microbial community. Inoculation with microbial antagonists may efficiently alleviate replanting issues. Through high-throughput sequencing, this study revealed that bacterial diversity decreased,whereas fungal diversity increased, in the rhizosphere soils of adult ginseng plants at the root growth stage under different ages. Few microbial community, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas,Sphingomonadaceae, and Zygomycota, were observed; the relative abundance of microorganisms, namely,Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota,increased in the soils of adult ginseng plants compared with those in the soils of 2-year-old seedlings. Bacillus subtilis 50-1, a microbial antagonist against the pathogenic Fusarium oxysporum, was isolated through a dual culture technique. These bacteria acted with a biocontrol efficacy of 67.8%. The ginseng death rate and Fusarium abundance decreased by 63.3% and 46.1%, respectively, after inoculation with B. subtilis 50-1. Data revealed that microecological degradation could result from ginseng-driven changes in rhizospheric microbial communities;these changes are associated with the different ages and developmental stages of ginseng plants. Biocontrol using microbial antagonists alleviated the replanting problem. | Linlin Dong Jiang Xu Lianjuan Zhang Ruiyang Cheng Guangfei Wei He Su Juan Yang Jun Qian Ran Xu Shilin Chen | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 27 |
| 17 | Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction显示文摘AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. | Wei-Ping Qian Yue-Qiu Tan Ying Chen Ying Peng Zhi Li Guang-Xiu Lu Made C. Liu Hsiang-Fu Kung Ming-Ling He Li-Ka Shing | 2005 | World Journal of Gastroenterology2005，11，34： | 25 |
| 18 | Annual cost of illness of stomach and esophageal cancer patientsin urban and rural areas in China: A multi-center study显示文摘Objective: Stomach and esophageal cancer are imposing huge threats to the health of Chinese people whereas there were few studies on the financial burden of the two cancers.Methods: Costs per hospitalization of all patients with stomach or esophageal cancer discharged between September 2015 and August 2016 in seven cities/counties in China were collected, together with their demographic information and clinical details. Former patients in the same hospitals were sampled to collect information on annual direct non-medical cost, indirect costs and annual number of hospitalization. Annual direct medical cost was obtained by multiplying cost per hospitalization by annual number of hospitalization. Annual cost of illness(ACI)was obtained by adding the average value of annual direct medical cost, direct non-medical cost and indirect cost,stratified by sex, age, clinical stage, therapy and pathologic type in urban and rural areas. Costs per hospitalization were itemized into eight parts to calculate the proportion of each part. All costs were converted to 2016 US dollars(1 USD=6.6423 RMB).Results: Totally 19,986 cases were included, predominately male. Mean ages of stomach cancer and urban patients were lower than that of esophageal cancer and rural patients. ACI of stomach and esophageal cancer patients were $10,449 and $13,029 in urban areas, and $2,927 and $3,504 in rural areas, respectively. Greater ACI was associated with male, non-elderly patients as well as those who were in stage I and underwent surgeries.Western medicine fee took the largest proportion of cost per hospitalization.Conclusions: The ACI of stomach and esophageal cancer was tremendous and varied substantially among the population in China. Preferential policies of medical insurance should be designed to tackle with this burden and further reduce the health care inequalities. | Zhixun Yang Hongmei Zeng Ruyi Xia Qian Liu Kexin Sun Rongshou Zheng Siwei Zhang Changfa Xia He Li Shuzheng Liu Zhiyi Zhang Yuqin Liu Guizhou Guo Guohui Song Yigong Zhu Xianghong Wu Bingbing Song Xianzhen Liao Yanfang Chen Wenqiang Wei Guihua Zhuang Wanqing Chen | 2018 | Chinese Journal of Cancer Research2018，30，4： | 22 |
| 19 | Small molecule inhibitors targeting the PD-1/PD-L1 signaling pathway显示文摘Tumor cells form immune escape and subsequently obtain unlimited proliferation ability due to the abnormal immune surveillance mediated by immune checkpoints.Among this class of immune checkpoints,PD-1/PD-L1 was recognized as an anticancer drug target for many years,and so far,several monoclonal antibodies have achieved encouraging outcome in cancer treatment by targeting the PD-1/PD-L1 signaling pathway.Due to the inherent limitations of antibodies,the development of small molecule inhibitors based on PD-1/PD-L1 signaling pathway is gradually reviving in decades.In this review,we summarized a number of small molecule inhibitors based on three different therapeutic approaches interfering PD-1/PD-L1 signaling pathway:(1)blocking direct interaction between PD-1 and PD-L1;(2)inhibiting transcription and translation of PD-L1;and(3)promoting degradation of PD-L1 protein.The development of these small molecule inhibitors opens a new avenue for tumor immunotherapy based on PD-1/PD-L1 signaling pathway. | Qian Wu Li Jiang Si-cheng Li Qiao-jun He Bo Yang Ji Cao | 2021 | Acta Pharmacologica Sinica2021，42，1： | 22 |
| 20 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
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