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| 1 | Activity after Site-Directed Mutagenesis of CD59 on Complement-Mediated Cytolysis显示文摘CD59 may inhibit the cytolytic activity of complement by binding to C8/C9 and protect host cell membranes against homologous membrane attack complex (MAC). However,CD59 is widely overexpressed on tumor cells,which has been implicated in tumorigenesis. The active site of CD59 relative to MAC is still confused. As reported the MAC binding site is located in the vicinity of a hydrophobic groove on the membrane distal face of the protein centered around residue W40. Here two site-directed mutagenesis were performed by overlapping extension PCR to delete residue W40 site (Mutant 1,M1) or to change C39W40K41 to W39W40W41 (Mutant 2,M2). Then we constructed mutant CD59 eukaryotic expression system and investigated their biological function on CHO cells compared with wild-type CD59. Stable populations of CHO cells expressing recombinant proteins were screened by immunotechnique. After 30 passages culturing,proteins could be tested. Dye release assays suggest that M1CD59 loses the activity against complement,while M2CD59 increases the anti-complement activity slightly. Results indicate that W40 of human CD59 is important to its activity,and prohibition of this site may be a potential way to increase complement activity and to treat tumors. | Xinhong Zhu Meihua Gao Shurong Ren Qiubo Wang Cunzhi Lin | 2008 | Cellular & Molecular Immunology2008，5，2： | 8 |
| 2 | NF-κB and FosB mediate inflammation and oxidative stress in the blast lung injury of rats exposed to shock waves显示文摘Blast lung injury(BLI)is the major cause of death in explosion-derived shock waves;however,the mechanisms of BLI are not well understood.To identify the time-dependent manner of BLI,a model of lung injury of rats induced by shock waves was established by a fuel air explosive.The model was evaluated by hematoxylin and eosin staining and pathological score.The inflammation and oxidative stress of lung injury were also investigated.The pathological scores of rats’lung injury at 2 h,24 h,3 days,and 7 days post-blast were 9.75±2.96,13.00±1.85,8.50±1.51,and 4.00±1.41,respectively,which were significantly increased compared with those in the control group(1.13±0.64;P<0.05).The respiratory frequency and pause were increased significantly,while minute expiratory volume,inspiratory time,and inspiratory peak flow rate were decreased in a time-dependent manner at 2 and 24 h post-blast compared with those in the control group.In addition,the expressions of inflammatory factors such as interleukin(IL)-6,IL-8,FosB,and NF-κB were increased significantly at 2 h and peaked at 24 h,which gradually decreased after 3 days and returned to normal in 2 weeks.The levels of total antioxidant capacity,total superoxide dismutase,and glutathione peroxidase were significantly decreased 24 h after the shock wave blast.Conversely,the malondialdehyde level reached the peak at 24 h.These results indicated that inflammatory and oxidative stress induced by shock waves changed significantly in a time-dependent manner,which may be the important factors and novel therapeutic targets for the treatment of BLI. | Hong Wang Wenjuan Zhang Jinren Liu Junhong Gao Le Fang Zhiyong Liu Baoqing Xia Xiaolin Fan Cunzhi Li Qing Lu Airong Qian | 2021 | Acta Biochimica et Biophysica Sinica2021，53，3： | 4 |
| 3 | A Hysteresis Model for Magneto-Rheological Damper显示文摘 | Shaopu Yang Cunzhi Pan | 2005 | International Journal of Nonlinear Sciences and Numerical Simulation2005，6，2： | 1 |
| 4 | Analysis of an Isolation System with Magnetorheological Damper显示文摘 | Shuqi Guo Cunzhi Pan Shaopu Yang | 2005 | International Journal of Nonlinear Sciences and Numerical Simulation2005，6，1： | 1 |
| 5 | Effect of Acupuncture treatment on vascular dementia显示文摘 | Yu Jianchun Zhang Xuezhu Liu Cunzhi | 2006 | Neurol Res2006，28，1： | 1 |
| 6 | Dynamic Modeling of Magnetorheological Damper behaviors 显示文摘 | Shuqi G Shaopu Y Cunzhi P | 2006 | Journal of Intelligent material systems and structures2006，17，1： | 1 |
| 7 | Some results on the complex oscillation theory of second order linear differential equations显示文摘 | Huang Cunzhi | 1991 | Kodai Math1991，14，： | 1 |
| 8 | Effect of acupuncture treatment on vascular dementia显示文摘 | JIANCHUN YU XUEZHU ZHANG CUNZHI LIU | 2006 | Neurological Research2006，28，： | 1 |
| 9 | Dynamic modeling of magneto-rheological damper behaviors 显示文摘 | Guo Shuqi Yang Shaopu Pan Cunzhi | 2006 | Journal of Intelligent Material Systems and Structures2006，17，1： | 1 |
| 10 | Nonlinear dynamics of a spur gear pair with time ~ varying stiffness and back-lash显示文摘 | Shen Yongjun Yang Shaopu Pan Cunzhi ea tl | 2004 | Journal of Low Frequency Noise Vibration and Active Control2004，23，3： | 1 |
| 11 | Effect of Acupuncture Treatment on Vascular Dementia显示文摘 | Jianchun Yu Xuezhu Zhang Cunzhi Liu | 2006 | Neurological research2006，28，7： | 1 |
| 12 | Effect of Acupuncture treatment on vascular dementia显示文摘 | Yu Jianchun Zhang Xuezhu Liu Cunzhi | 2006 | Neurol Res2006，28，1： | 1 |
| 13 | Acupuncture prevents cognitive deficits and oxidative stress in cerebral multi-infarction rats显示文摘 | Liu Cunzhi Yu Jianchun Zhang Xuezhu | 2006 | Neurosci Lett2006，393，1： | 1 |
| 14 | Dynamic modeling of magnetorheological damper behaviors显示文摘 | Shuqi G Shaopu Y Cunzhi P | 2006 | Journal of Intelligent ma- terial systems and structures2006，17，1： | 1 |
| 15 | The Fuzzy Synthesizing Evaluation of City's Environmental Quality Based on GIOWA Operator:A Case of 13 Cities of Jiangsu Province显示文摘In this paper, the generally induced ordered weighted averaging (GIOWA) operator is used to construct the fuzzy evaluating system of the quality of a city’s environment including air, water and noise. Based on the part data of air, water and noise of the 13 cities under the jurisdiction of Jiangsu provincial government in 2002, a case study is given. The evaluating results show that the evaluating system of the quality of a city’s environment based on the GIOWA operator can reflect the condition of pollution controlled and environmental quality of a city in a period of time. | Bai Xianchun 1,2, Ling Kang 2 & Guo Cunzhi 2 1. Institute of Management Science and Engineering, Jinan University, Guangzhou 510632, China 2. Nanjing University of Finance and Economics, School of Economics, Nanjing 210003, China | 2005 | Chinese Journal of Population,Resources and Environment2005，3，4： | 1 |
| 16 | Dynamic modeling of magnetorheological damper behaviors 显示文摘 | Guo Shuqi Yang Shaopu Pan Cunzhi | 2006 | Journal of Intelligent Material Systems and Structures2006，17，1： | 1 |
| 17 | Serum and tissue levels of six trace elements and copper/zinc ratio in patients with cervical cancer and uterine myoma显示文摘 | Cunzhi H Jiexian J Xianwen Z | 2003 | Biol Trace Elem Res2003，94，2： | 1 |
| 18 | Serum levels of leptin, insulin, and lipids in relation to breast cancer in China显示文摘 | Cunzhi Han Hong-Tao Zhang Lili Du Xiuying Liu Jiexian Jing Xianwen Zhao Xuan Yang Baoguo Tian | 2005 | Endocrine2005，，1： | 1 |
| 19 | Acupuncture improved cognitive impairment caused by multi-infarct dementia in rats显示文摘 | Jianchun Yu Cunzhi Liu | 2005 | Physiology & Behavior2005，86，： | 1 |
| 20 | New method of circle and arc cutting显示文摘 | Qin Hua Sun Cunzhi | 2012 | Optik2012，123，17： | 1 |
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