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| 1 | Virus like particle-based vaccines against emerging infectious disease viruses显示文摘Emerging infectious diseases are major threats to human health.Most severe viral disease outbreaks occur in developing regions where health conditions are poor.With increased international travel and business,the possibility of eventually transmitting infectious viruses between different countries is increasing.The most effective approach in preventing viral diseases is vaccination.However,vaccines are not currently available for numerous viral diseases.Viruslike particles(VLPs) are engineered vaccine candidates that have been studied for decades.VLPs are constructed by viral protein expression in various expression systems that promote the selfassembly of proteins into structures resembling virus particles.VLPs have antigenicity similar to that of the native virus,but are non-infectious as they lack key viral genetic material.VLP vaccines have attracted considerable research interest because they offer several advantages over traditional vaccines.Studies have shown that VLP vaccines can stimulate both humoral and cellular immune responses,which may offer effective antiviral protection.Here we review recent developments with VLP-based vaccines for several highly virulent emerging or re-emerging infectious diseases.The infectious agents discussed include RNA viruses from different virus families,such as the Arenaviridae,Bunyaviridae,Caliciviridae,Coronaviridae,Filoviridae,Flaviviridae,Orthomyxoviridae,Paramyxoviridae,and Togaviridae families. | Jinliang Liu Shiyu Dai Manli Wang Zhihong Hu Hualin Wang Fei Deng | 2016 | Virologica Sinica2016，31，4： | 7 |
| 2 | Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice显示文摘The newly emerged mosquito-borne Zika virus(ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. Several ZIKV vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, DNA and RNA vaccines. These have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. Virus-like particles(VLPs) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies. In this study, we used a new strategy to produce ZIKV VLPs based on the baculovirus expression system and demonstrated the feasibility of their use as a vaccine candidate. The pre-membrane(prM) and envelope(E) proteins were co-expressed in insect cells and selfassembled into particles similar to ZIKV. We found that the ZIKV VLPs could be quickly and easily prepared in large quantities using this system. The VLPs were shown to have good immunogenicity in immunized mice, as they stimulated high levels of virus neutralizing antibody titers, ZIKV-specific IgG titers and potent memory T cell responses. Thus, the baculovirus-based ZIKV VLP vaccine is a safe, effective and economical vaccine candidate for use against ZIKV. | Shiyu Dai Tao Zhang Yanfang Zhang Hualin Wang Fei Deng | 2018 | Virologica Sinica2018，33，3： | 5 |
| 3 | Microstructure and Mechanical Properties of AZ31 Alloys Processed by Residual Heat Rolling显示文摘To produce high strength and ductility Mg alloys with high productivity and low energy consumption,the residual heat rolling (RHR) process was initially proposed.The microstucture and mechanical properties of AZ31 processed by RHR were investigated by optical micrscopy (OM),electron backscatter diffraction (EBSD),and electron universal testing machine.The yield strength (YS),ultimate tensile strength (UTS),and elongation to failure of RHRed AZ31 sheet were 194 MPa,311 MPa,and 22%,respectively.The RHRed AZ31 alloys after annealing have very fine and homogeneous grains.The symmetrical rolled (SR) and RHRed AZ31 exhibit typical {0002} basal textures.The RHRed AZ31 has double-peak basal texture distribution.The basal poles of RHRed AZ31 split from normal direction (ND) to rolling direction (RD).There are few hard orientation distributions on the basal slip and more soft orientation distributions on the prismatic slip in the RHRed AZ31 sheets than those in the SRed AZ31sheets. | 刘杨 ZHAO Yiquan 王岭 JIN Xiuying SUN Chao WANG Xiaomin WANG Gang DAI Shiyu WANG Yinong | 2021 | Journal of Wuhan University of Technology(Materials Science)2021，36，4： | 3 |
| 4 | A novel PIK3CD C896T mutation detected in bilateral sudden sensorineural hearing loss using next generation sequencing:An indication of primary immunodeficiency显示文摘Objective: To investigate immune-related genetic background in bilateral sudden sensorineural hearing loss(SSNHL).Case report and methods: The case is a 45-year-old man presenting with a 7-year history of bilateral profound SSNHL. Blood biochemical testing demonstrated increased levels of total cholesterol(5.88 mmol/L). Tests for hepatitis B showed a positive antibody against the hepatitis B core antigen. Complement C3 was below the normal value, and complement C4 and Ig G were in the lower range of normal values. CT images showed a normal inner ear and vestibular aqueduct but round window membranous ossification on both sides. A total number of 232 immuneassociated genes were sequenced using the next generation sequencing technique.Results: Mutations were detected in 5 genes, including the phosphoinositide 3-kinase catalytic subunit delta(PIK3CD), caspase recruitment domain-containing protein 9(CARD9), complement factor H-related(CFHR2), immunoglobulin lambda-like polypeptide 1 Protein(IGLL1),and transmembrane channel-like gene family 8(TMC8). In the PIK3 CD gene, a C896 T substitute in exon 7 was detected. This mutation causes primary immunodeficiency and is an autosomal dominant disease.Conclusion: The PIK3 CD C896T mutation responsible for primary immunodeficiency may contribute to the onset of bilateral SSNHL with subsequent rapid progression. | Jing Zou Xiangqiang Duan Guiliang Zheng Zhen Zhao Shiyue Chen Pu Dai Hongliang Zheng | 2016 | Journal of Otology2016，11，2： | 3 |
| 5 | Transformation of Laves phases and its effect on the mechanical properties of TIG welded Mg-Al-Ca-Mn alloys显示文摘The effects of Al content and Ca/Al mass ratio on the microstructure and mechanical properties of tungsten inert gas(TIG)welded Mg-2Ca-x Al-0.5Mn(x=0,1,5)alloy joints were studied in present work.Results showed that increasing Al content was effective in reducing the dendrite spacing at the fusion zone(FZ)edge.The Laves phases in the FZ and the heat-affected zone(HAZ)can be changed from Mg_(2)Ca to(Mg,Al)_(2)Ca with the decrease of Ca/Al ratio,and the(Mg,Al)_(2)Ca could be further transformed to Al_(2)Ca under welding thermal cycle.Furthermore,dynamic dissolution and precipitation of Laves phases and Al_(8)Mn_(5)phases occurred in the HAZ,resulting in a gradient microstructure and hardness peak in this area.The tensile properties of the joints were significantly improved with the increase of Al content,which was mainly due to the modification of Laves phases. | Sensen Chai Shiyu Zhong Qingshan Yang Daliang Yu Qingwei Dai Hehe Zhang Limeng Yin Gang Wang Zongxiang Yao | 2022 | Journal of Materials Science & Technology2022，，25： | 2 |
| 6 | A dense graphene monolith with poloxamer prefunctionalization enabling aqueous redispersion to obtain solubilized graphene sheets显示文摘The realization of good aqueous dispersibility of commercial graphene products composed of exfoliated graphene sheets is of significance for downstream applications.However,the tap density of commercial graphene powder is quite low(0.03-0.1 kg/m3),meaning that 1 kg graphene powder occupies about 10-30 m3 in volume during transportation.And,the available content of commercial graphene dispersion/slurry in aqueous medium cannot exceed 5 wt%,although the density is high(≈1050 kg/m3).In this work,a graphene monolith was prepared by oven-drying of graphene sheets prefunctionalized with poloxamer surfactants.Our graphene monoliths not only have a high density(1500 kg/m^3) and high graphene content(≈10 wt%),but also a full capability to be completely redispersed(≈100%) in water by bath sonication to obtain solubilized graphene sheets,whose lateral size and thickness are unchanged compared to as-exfoliated ones.Moreover,a simple empirical method was proposed to predict the redispersion capability of graphene monoliths using different poloxamers by contact angle measurements.Our results provide a universal approach to make exfoliated graphene-based products with better downstream availability and lower transportation cost. | Chen Ye Fan Zhang Xue Tan Huifang Sun Wen Dai Ke Yang Minghui Yang Shiyu Du Dan Dai Jinhong Yu Nan Jiang Weitao Su Li Fu He Li Jing Kong Cheng-Te Lin | 2020 | Chinese Chemical Letters2020，31，9： | 0 |
| 7 | The application of molecular simulation in ash chemistry of coal显示文摘One of the crucial issues in modern ash chemistry is the realization of efficient and clean coal conversion.Industrially,large-scale coal gasification technology is well known as the foundation to improve the atom economy.In practice,the coal ash fusibility is a critical factor to determine steady operation standards of the gasifier,which is also the significant criterion to coal species selection for gasification.Since coal behaviors are resultant from various evolutions in different scales,the multi-scale understanding of the ash chemistry is of significance to guide the fusibility adjustment for coal gasification.Considering important roles of molecular simulation in exploring ash chemistry,this paper reviews the recent studies and developments on modeling of molecular systems for fusibility related ash chemistry for the first time.The discussions are emphasized on those performed by quantum mechanics and molecular mechanics,the two major simulation methods for microscopic systems,which may provide various insights into fusibility mechanism.This review article is expected to present comprehensive information for recent molecular simulations of coal chemistry so that new clues to find strategies controlling the ash fusion behavior can be obtained. | Xin Dai Jin Bai Ping Yuan Shiyu Du Dongtao Li XiaodongWen Wen Li | 2020 | Chinese Journal of Chemical Engineering2020，28，11： | 0 |
| 8 | SERPINE2 promotes liver cancer metastasis by inhibiting c-Cbl-mediated EGFR ubiquitination and degradation显示文摘Background:Liver cancer is a malignancy with high morbidity and mortality rates.Serpin family E member 2(SERPINE2)has been reported to play a key role in the metastasis of many tumors.In this study,we aimed to investigate the potential mechanism of SERPINE2 in liver cancer metastasis.Methods:The Cancer Genome Atlas database(TCGA),including DNA methy-lation and transcriptome sequencing data,was utilized to identify the crucial oncogene associated with DNA methylation and cancer progression in liver can-cer.Data from the TCGA and RNA sequencing for 94 pairs of liver cancer tissues were used to explore the correlation between SERPINE2 expression and clin-ical parameters of patients.DNA methylation sequencing was used to detect the DNA methylation levels in liver cancer tissues and cells.RNA sequencing,cytokine assays,immunoprecipitation(IP)and mass spectrometry(MS)assays,protein stability assays,and ubiquitination assays were performed to explore the regulatory mechanism of SERPINE2 in liver cancer metastasis.Patient-derived xenografts and tumor organoid models were established to determine the role of SERPINE2 in the treatment of liver cancer using sorafenib.Results:Based on the public database screening,SERPINE2 was identified as a tumor promoter regulated by DNA methylation.SERPINE2 expression was significantly higher in liver cancer tissues and was associated with the dismal prognosis in patients with liver cancer.SERPINE2 promoted liver cancer metas-tasis by enhancing cell pseudopodia formation,cell adhesion,cancer-associated fibroblast activation,extracellular matrix remodeling,and angiogenesis.IP/MS assays confirmed that SERPINE2 activated epidermal growth factor receptor(EGFR)and its downstream signaling pathways by interacting with EGFR.Mechanistically,SERPINE2 inhibited EGFR ubiquitination and maintained its protein stability by competing with the E3 ubiquitin ligase,c-Cbl.Additionally,EGFR was activated in liver cancer cells after sorafenib treatment,and SER-PINE2 knockdown-induced EGFR downregulation significantly enhanced the therapeutic efficacy of sorafenib against liver cancer.Furthermore,we found that SERPINE2 knockdown also had a sensitizing effect on lenvatinib treatment.Conclusions:SERPINE2 promoted liver cancer metastasis by preventing EGFR degradation via c-Cbl-mediated ubiquitination,suggesting that inhibition of the SERPINE2-EGFR axis may be a potential target for liver cancer treatment. | Shiyu Zhang Xing Jia Haojiang Dai Xingxin Zhu Wenfeng Song Suchen Bian Hao Wu Shinuo Chen Yangbo Tang Junran Chen Cheng Jin Mengqiao Zhou Haiyang Xie Shusen Zheng Penghong Song | 2024 | Cancer Communications2024，44，3： | 0 |
| 9 | Microstructures,mechanical properties and degradability of Mg-2Gd-0.5(Cu/Ni)alloys:A comparison study显示文摘Low alloying Mg-2 Gd-0.5(Cu/Ni)alloys for sealing tools in the oil and gas industry were prepared.The differences in the effects of minor Cu and Ni additions on microstructures and properties of the Mg-2 Gd alloy were compared.The results showed that adding Ni was more effective than adding Cu in refining grain sizes,strengthening the basal fiber texture,and promoting the formation of LPSO phases,resulting in higher strength.The tensile yield strength/elongation of the Mg-2 Gd-0.5 Cu alloy,Mg-2 Gd-0.25 Cu-0.25 Ni alloy,and Mg-2 Gd-0.5 Ni alloy was 146 MPa/23.7%,175 MPa/23.1%,and 248 MPa/18.2%,respectively.The decreased elongation was attributed to the basal fiber texture and the presence of coarse LPSO phases.In terms of the corrosion rate in 3.5 wt.%NaCl solution,it rose from 112 mm y^(-1)for the Mg-2 Gd-0.5 Cu alloy to 269 mm y^(-1)for the Mg-2 Gd-0.25 Cu-0.25 Ni alloy and 490 mm y^(-1)for the Mg-2 Gd-0.5 Ni alloy,indicating that the addition of Ni instead of Cu showed a more significant promoting effect on the degradability of Mg alloys,which was related to more refined grains,the stronger basal fiber texture,and a larger amount of LPSO phases. | Shiyu Zhong Dingfei Zhang Yongqin Wang Sensen Chai Jingkai Feng Yulun Luo Jianrong Hua Qimin Dai Guangshan Hu Junyao Xu Bin Jiang Fusheng Pan | 2022 | Journal of Materials Science & Technology2022，，33： | 0 |
| 10 | Layer-by-layer stacked graphene nanocoatings by Marangoni self-assembly for corrosion protection of stainless steel显示文摘Graphene nanosheets are widely used in anti-corrosion polymeric coating as filler,owing to the excellent electrochemical inertness and barrier property.However,as the arrangement of graphene nanosheets is difficult to form a perfect layered structure,polymeric coating with graphene nanosheets usually needs micron-scale thickness to ensure the enhancement of corrosion protection.In this work,layer-by-layer stacked graphene nanocoatings were fabricated on stainless steel by self-assembly based on Marangoni effect.The anti-corrosion property of graphene coatings were studied through Tafel polarization curves,electrochemical impedance spectroscopy and accelerated corrosion test with extra applied voltage.The self corrosion current density of optimized three-layered graphene coated sample was one quarter of that of bare stainless steel.And the self corrosion potential of optimized sample is increased to-0.045 V.According to the results,graphene nanocoatings composed of layered nanosheets exhibits good anticorrosion property.Besides,the self-assembly method provide a promising approach to make layeredstructure coating for other researches about 2 D material nanosheets. | Chen Ye Yangguang Zhu Hongyan Sun Feiyue Chen Huifang Sun Wen Dai Qiuping Wei Li Fu Aimin Yu Shiyu Du Minghui Yang Liang-Feng Huang Jinhong Yu Nan Jiang Cheng-Te Lin | 2021 | Chinese Chemical Letters2021，32，1： | 0 |
| 11 | Transcriptome profiling highlights regulated biological processes and type III interferon antiviral responses upon Crimean-Congo hemorrhagic fever virus infection显示文摘Crimean-Congo hemorrhagic fever virus(CCHFV)is a biosafety level-4(BSL-4)pathogen that causes Crimean-Congo hemorrhagic fever(CCHF)characterized by hemorrhagic manifestation,multiple organ failure and high mortality rate,posing great threat to public health.Despite the recently increasing research efforts on CCHFV,host cell responses associated with CCHFV infection remain to be further characterized.Here,to better understand the cellular response to CCHFV infection,we performed a transcriptomic analysis in human kidney HEK293 cells by high-throughput RNA sequencing(RNA-seq)technology.In total,496 differentially expressed genes(DEGs),including 361 up-regulated and 135 down-regulated genes,were identified in CCHFV-infected cells.These regulated genes were mainly involved in host processes including defense response to virus,response to stress,regulation of viral process,immune response,metabolism,stimulus,apoptosis and protein catabolic process.Therein,a significant up-regulation of type III interferon(IFN)signaling pathway as well as endoplasmic reticulum(ER)stress response was especially remarkable.Subsequently,representative DEGs from these processes were well validated by RT-qPCR,confirming the RNA-seq results and the typical regulation of IFN responses and ER stress by CCHFV.Furthermore,we demonstrate that not only type I but also type III IFNs(even at low dosages)have substantial anti-CCHFV activities.Collectively,the data may provide new and comprehensive insights into the virus-host interactions and particularly highlights the potential role of type III IFNs in restricting CCHFV,which may help inform further mechanistic delineation of the viral infection and development of anti-CCHFV strategies. | Qiong Mo Kuan Feng Shiyu Dai Qiaoli Wu Zhong Zhang Ashaq Ali Fei Deng Hualin Wang Yun-Jia Ning | 2023 | Virologica Sinica2023，38，1： | 0 |
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