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		题名	作者	年代	出处	被引量
	1	Expression of Single‐Chain Fv Gene Specific for γ‐Seminoprotein by RTS and Its Biological Activity Identification显示文摘	YuedongHan YiHaun JinlanDeng FengGao BifengPan DaxiangCui	2008	Biotechnol Progress2008，，4：	1
	2	Cloning and expression of the ureC geneo f H.pylori显示文摘AIM: To express Hp ureC gene in E.coli, a fragment from Hp ureC was amplified by the polymerase chain reaction and was cloned into E.coli expressing plasmid vector--- pBV220. This study lay the foundation for further studies of its functions. METHODS : An 1173bp gene fragment was amplified by the polymerase chain reaction. The template DNA was purified from Hp clinical strain. The gene fragment was digested by BamH I and EcoR I and cloned into pGEM-3Zf (-) plasmid. Its sequence was determined by autosequencing instrument from two directions. Then the gene fragment was cloned into pBV220. Subsequently the DH5 a was transformed by pBV220/ureC and was induced by heat when temperature reached 42'C. The form of expressed protein was analyzed to learn that in which form the recombinant protein was expressed. The thin layer chromatogram was used to scan thegel to learn that the quantity of the expressed protein. Antigenicity of the expressed protein wasconfirmed by ELISA and DIGFA. RESULTS z 95% of the DNA sequence we got is the same as that ofHp strain M60398. A Mr 44000 recombinant protein was induced by heat when temperature reached 42℃. The molecular mass of the expressed ureC gene is coincide with expected. The expressed proteinaccounts for 24.8% of the whole protein of the host bactria. Although most of the recombinant proteinsare inclusion bodies in host bactria after translation, there does exist certain quantity of solvable proteins in the supernatant of bacteria lysate. CONCLUSION:Recombinant H.pylori urease C protein is expressed in E.coli and the expressed protein has the antigenicity ofureC. Our study lays the foundation for further studies of the functions of lip ureC.	JunRongXu LiZhang XiaoJunYan DaXiangCui HongJiang FengChanHan	2001	世界感染杂志2001，1，1：	0

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