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| 1 | Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome显示文摘AIM:To design and validate broad-range 16S rRNA primers for use in high throughput sequencing to classify bacteria isolated from the human foregut microbiome.METHODS:A foregut microbiome dataset was constructed using 16S rRNA gene sequences obtained from oral,esophageal,and gastric microbiomes produced by Sanger sequencing in previous studies represented by 219 bacterial species.Candidate primers evaluated were from the European rRNA database.To assess the effect of sequence length on accuracy of classification,16S rRNA genes of various lengths were created by trimming the full length sequences.Sequences spanning various hypervariable regions were selected to simulate the amplicons that would be obtained using possible primer pairs.The sequences were compared with full length 16S rRNA genes for accuracy in taxonomic classification using online software at the Ribosomal Database Project (RDP).The universality of the primer set was evaluated using the RDP 16S rRNA database which is comprised of 433 306 16S rRNA genes,represented by 36 phyla.RESULTS:Truncation to 100 nucleotides(nt)downstream from the position corresponding to base 28 in the Escherichia coli 16S rRNA gene caused misclassification of 87(39.7%)of the 219 sequences,compared with misclassification of only 29(13.2%)sequences with truncation to 350 nt.Among 350-nt sequence reads within various regions of the 16S rRNA gene,the reverse read of an amplicon generated using the 343F/798R primers had the least(8.2%)effect on classification.In comparison,truncation to 900 nt mimicking single pass Sanger reads misclassified 5.0%of the 219 sequences.The 343F/798R amplicon accurately assigned 91.8%of the 219 sequences at the species level.Weighted by abundance of the species in the esophageal dataset,the 343F/798R amplicon yielded similar classification accuracy without a significant loss in species coverage(92%).Modification of the 343F/798R primers to 347F/803R increased their universality among foregut species.Assuming that a typicalpolymerase chain reaction can tolerate 2 mismatches between a primer and a template,the modified 347F and 803R primers should be able to anneal 98%and 99.6%of all 16S rRNA genes in the RDP database.CONCLUSION:347F/803R is the most suitable pair of primers for classification of foregut 16S rRNA genes but also possess universality suitable for analyses of other complex microbiomes. | Carlos W Nossa William E Oberdorf Jφrn A Aas Bruce J Paster Todd Z DeSantis Eoin L Brodie Daniel Malamud Michael A Poles Zhiheng Pei | 2010 | World Journal of Gastroenterology2010，16，33： | 16 |
| 2 | Outcomes after arthroscopic repair of rotator cuff tears in the setting of mild to moderate glenohumeral osteoarthritis显示文摘BACKGROUND Rotator cuff pathology is a very common source of shoulder pain.Similarly,osteoarthritis of the glenohumeral joint can cause shoulder pain and produce similar symptoms.Surgical management can be indicated for both pathologies,however,outcomes data is limited when examining rotator cuff repair(RCR) in the setting of glenohumeral arthritis(GHOA).Thus,this study sought to determine outcomes for patients who undergo RCR in the setting of GHOA.AIM To evaluate if a relationship exists between outcomes of RCR in the setting of GHOA.METHODS This was a retrospective analysis of patients who underwent arthroscopic rotator cuff repair with concurrent glenohumeral osteoarthritis between 2010-2017.Patients were stratified based on rotator cuff tear size and glenohumeral osteoarthritis severity.Cohorts were paired 1:1 with patients without glenohumeral osteoarthritis.Patients included had a minimum two year follow-up.Rate of conversion to total shoulder arthroplasty,complication rates following initial surgery,and patient-reported outcome measures were collected.RESULTS A total of 142 patients were included.The number of patients that required total shoulder arthroplasty within two years after index surgery was low.2/71(2.8%) patients with GHOA,and 1/71(1.4%) without GHOA.Following rotator cuff repair,both groups showed favorable patientreported outcomes.CONCLUSION Patients with glenohumeral osteoarthritis who underwent arthroscopic rotator cuff repair showed comparable outcomes to patients without glenohumeral osteoarthritis. | Ian S Hong Allison J Rao Tyler L CarlLee Joshua D Meade Daniel J Hurwit Gregory Scarola David P Trofa Shadley C Schiffern Nady Hamid Patrick M Connor James E Fleischli Bryan Michael Saltzman | 2022 | World Journal of Orthopedics2022，13，7： | 2 |
| 3 | Levels of vascular endothelial growth factor are elevated in patients with ectopic pregnancy:is this a novel marker显示文摘 | Daniel Y Geva E Lerner-Geva L | 1999 | Fertil Steril1999，72，6： | 1 |
| 4 | Association of a renal papillary carcinoma with a low grade tumor of the collecting ducts显示文摘 | Daniel L Zattara Cannoni H Lechevallier E | 2001 | J Clin Pathol2001，54，8： | 1 |
| 5 | The anatomy of tissue cultured red raspberry prior to and after transfer to soil 显示文摘 | Danielle J D William E V Kwai Y L | 1985 | Plant Cell: Tissue and Organ Culture1985，，1： | 1 |
| 6 | Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2012-2013显示文摘 | Meijer A Rebelo-de-Andrade H Correia V Besselaar T Drager-Dayal R Fry A Gregory V Gubareva L Kageyama T Laekenby A Lo J Odagiri T Pere- yaslov D Siqueira M M Takashita E Tashiro M Wang D Wong S Zhang W Daniels R S Hurt A C | 2014 | An- tiviral Research2014，110，： | 1 |
| 7 | Incorporation of alkoxysilanes into model latex systems: viny copolymerizatio,a of vinyltriethoxysilane and n-butyl acrylate 显示文摘 | Marcu I Daniels E S Dimonie V L | 2003 | Macromolecules2003，36，： | 1 |
| 8 | Chemically grafted polymeric filters for chemical sensors: hyperbranched poly ( acrylic acid) films incorporatingh - cyclodextrin receptors and amine - functionalized filter layers 显示文摘 | Daniel L Dermody Robert F Peez D E | 1999 | Langmuir1999，15，3： | 1 |
| 9 | Removal of ammonium via simultaneous nitrification-denitrification nitrite-shortcut in a single packed-bed batch reactor显示文摘 | Daniel L M C Pozzi E Foresti E | 2009 | Bioresource Technology2009，100，： | 1 |
| 10 | The anatomy of tissue cultured red raspberry prior to and after transfer to soil显示文摘 | Danielle J D William E V Kwai Y L | 1985 | Plant Cell( Tissue and Organ Culture)1985，，1： | 1 |
| 11 | Nonpoint Source 显示文摘 | Daniel E L Richard A M | 1998 | Water Environment Research1998，70，4： | 1 |
| 12 | Interactive inverse design op-timization of fuselage shape for low boom supersonic con-cepts显示文摘 | Li W Shieds E Daniel L | 2008 | Journal of Aircraft2008，45，9： | 1 |
| 13 | The concept of wage-push inflation : Development and policy 显示文摘 | Daniel J B Mitchell C L E | 2008 | Labor History2008，4，49： | 1 |
| 14 | 难治性类风湿关节炎的临床特点:一项国际问卷调查研究显示文摘目的尽管按照目前欧洲抗风湿联盟(EULAR)的推荐对类风湿关节炎(rheumatoid arthritis,RA)患者进行治疗,难治性RA患者的临床症状却仍然没有得到完全控制,目前EULAR指南主要关注的是RA的早期治疗和药物治疗。本研究的目的在于分析难治性RA患者的临床特点,以及在患者管理中需要探索然而现有EULAR指南并未涉及的问题。方法对多个国家的风湿科医生进行问卷调查,分析难治性RA的临床特点。问卷包括多选题以及开放性问题,从开放性问题的回答中发现需要进一步阐明的问题以及现有EULAR指南需要增加的内容。结果410名受访者完成了调查问卷,关于难治性RA的特点:50%受访者认为基于28个关节评估的疾病活动评分(disease activity score assessing 28 joints,DAS28)>3.2或存在疾病活动的征象;42%受访者认为疲劳是其特点;48%认为至少两种传统合成改变病情抗风湿药(DMARDs)以及使用至少两种生物制剂或靶向合成DMARDs治疗失败;89%认为糖皮质激素不能减量到5 mg/天或泼尼松不能减量到10 mg/天或同等剂量的其他激素。另外,现有EULAR指南中应增加合并症和关节外表现的干预以及多种药物联合治疗等重要内容。结论目前风湿科医生对难治性RA的定义有很大争议。难治性RA患者的很多重要问题目前EULAR指南中均没有涉及。 | Nadia MT Roodenrijs Maria J H de Hair Marlies C van der Goes Johannes WG Jacobs Paco MJ Welsing Désirée van der Heijde Daniel Aletaha Maxime Dougados Kimme L Hyrich Iain B McInnes Ulf Mueller-Ladner Ladislav Senolt Zoltan Szekanecz Jacob Mvan Laar Nagy Gyorgy 黄艳荣(译) 张卓莉(审校) | 2019 | 英国医学杂志中文版2019，22，2： | 1 |
| 15 | Bt toxin resistance from loss of a putative carbohydrate2 modifying enzyme 显示文摘 | Joel S G Johanna L W Daniel E S | 2001 | Science2001，293，3： | 1 |
| 16 | A computerized method of visual acuity testing显示文摘 | Roy W Beck Pamela S Moke Andrew H Turpin Frederick L Ferris John Paul SanGiovanni Chris A Johnson Eileen E Birch Danielle L Chandler Terry A Cox R.Clifford Blair Raymond T Kraker | 2003 | American Journal of Ophthalmology2003，，2： | 1 |
| 17 | Evolving morphotropic phase boundary in lead-free ( Bil/2 Nal/2 )TiO3 - BaTiO3 pi- ezoceramics 显示文摘 | JO W DANIELS J E JONES J L | 2011 | Journal of Applied Physics2011，109，01： | 1 |
| 18 | Tillage effects on soil properties and crop production in the sub-humid and semiarid Argentinean Pampas 显示文摘 | Daniel E B Jose L P Paul W U | 1998 | Soil & Tillage Res1998，49，： | 1 |
| 19 | Design of homogeneous,monopegylated erythropoietin analogs with preserved in vitro bioactivlty显示文摘 | Dana L L Daniel H D Stephen P E | 2006 | Cox Exp Hematology2006，34，6： | 1 |
| 20 | Cost-effectiveness of epidermal growth factor receptor mutation testing and first-line treatment with gefitinib for patients withadvanced adenocarcinoma of the lung显示文摘 | LopesGde L Jr Joel E Daniel S | 2012 | Cancer2012，118，4： | 1 |
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