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| 1 | A study on the spatial difference of farmland nitrogen nutrient budget in the Bohai Rim region, China显示文摘Bohai 边界区域是一个为在中国的商品谷物生产的最重要的底。与农业工业化的快速的步,含氮的化肥以曾经增加的率被使用了,它在土壤导致了积聚的氮的踪迹并且引起了严重环境问题。在这研究,我们使用了农田氮平衡模型与 GIS 的帮助在 2008 在 Bohai 边界区域估计农田氮营养素预算的空间差别。我们的结果显示了那:1 ) 在这个区域的农田有氮剩余总计 288.54 kg/ha 的 508.22 万吨,或一般水准。2 ) 在 Bohai 边界区域,农田氮输入和农田氮预算,两个显示出空间区别。主要生产谷物的区域比放牧耕作区域的有更高的氮输入。氮输入的主要来源包括化肥,器官的化肥,从大气的弄干并且弄湿的免职,和生物固定,它分别地占 79.47% , 9.53% , 4.62% ,和 3.58% 全部的输入。因此,化肥是到农田的氮输入的占优势的来源。3 )333.98 万大量氮的一个总数是从经由收获庄稼的农田的输出,它在这个区域从农田说明 52.36% 全部的氮输出。平均,来自统一农田的氮输出的数量等于 176.65kg/ha。这研究有在学习区域使农田氮预算和它的空间变化清楚些,可以为文饰提供科学证据化肥的使用和地区性的规模上的设法的农业操作并且为在地区性的规模改进化肥使用的经济、生态的效率也是珍贵的。 | GUO Liying YANG Ren WANG Daolong | 2012 | Journal of Geographical Sciences2012，22，4： | 5 |
| 2 | Targeting of anti-microbial proteins to the hyphal surface amplifies protection of crop plants against Phytophthora pathogens显示文摘Phytophthora pathogens are a persistent threat to the world's commercially important agricultural crops,including potato and soybean.Current strategies aim at reducing crop losses rely mostly on disease-resistance breeding and chemical pesticides,which can be frequently overcome by the rapid adaptive evolution of pathogens.Transgenic crops with intrinsic disease resista nee offer a promising alternative and con tinue to be developed.Here,we explored Phytophthora-derived PI3P(phosphatidylinositol 3-phosphate)as a novel control target,using proteins that bind this lipid to direct secreted anti-microbial peptides and proteins(AMPs)to the surface of Phytophthora pathogens.In transgenic Nicotiana benthamiana,soybean,and potato plants,significantly enhanced resistance to different pathogen isolates was achieved by expression of two AMPs(GAFP1 or GAFP3 from the Chinese medicinal herb Gastrodia elata)fused with a PI3P-specific binding domain(FYVE).Using the soybean pathogen P.sojae as an example,we demonstrated that the FYVE domain could boost the activities of GAFPs in multiple independent assays,including those performed in vitro,in vivo,and in planta.Mutational analysis of P.sojae PI3K1 and PI3K2 genes of this pathogen confirmed that the enhanced activities of the targeted GAFPs were correlated with PI3P levels in the pathogen.Collectively,our study provides a new strategy that could be used to confer resistance not only to Phytophthora pathogens in many plants but also potentially to many other kinds of plant pathogens with unique targets. | Yang Zhou Kun Yang Qiang Yan Xiaodan Wang Ming Cheng Jierui Si Xue Xue Danyu Shen Maofeng Jing Brett M.Tyler Daolong Dou | 2021 | Molecular Plant2021，14，8： | 2 |
| 3 | Simulation study on interaction coefficient of DEM in non-spherical large size (5-30 mm) coal particles显示文摘The pneumatic conveying system of coal particles can greatly reduce the dust and improve the environmental quality at underground mining workface and the surrounding of coal enterprises.The particle shape and the interaction coefficients between particles and the contact surface play important roles in the pneumatic conveying and CFD-DEM simulation.In order to build the semblable shape models and obtain the accurate interaction coefficients of large coal particles,this article establishes the con tact model by the particle overlap method and describes the mathematical model of the shape characteristics for large coal particle.The particle models are simulated by adopting the multi-index mixed orthogonal experiments.The accumulation density,the porosity and the error between simulation and experiment are taken as the indexes,and the particle models and the particle contact coefficients are taken as the orthogonal test factors.As a result,three more accurate particle models and their interaction coefficients are obtained,which provide the model basis for the pneumatic conveying of large coal particles. | Daolong Yang Yanxiang Wang Jianping Li Qingkai Wang Yuntao Wang Yanting Yu | 2020 | Particuology2020，18，6： | 1 |
| 4 | The Pythium periplocum elicitin PpEli2 confers broad-spectrum disease resistance by triggering a novel receptor-dependent immune pathway in plants显示文摘Elicitins are microbe-associated molecular patterns produced by oomycetes to elicit plant defense.It is still unclear whether elicitins derived from non-pathogenic oomycetes can be used as bioactive molecules for disease control.Here,for the first time we identify and characterize an elicitin named PpEli2 from the soil-borne oomycete Pythium periplocum,which is a non-pathogenic mycoparasite colonizing the root ecosystem of diverse plant species.Perceived by a novel cell surface receptor-like protein,REli,that is conserved in various plants(e.g.tomato,pepper,soybean),PpEli2 can induce hypersensitive response cell death and an immunity response in Nicotiana benthamiana.Meanwhile,PpEli2 enhances the interaction between REli and its co-receptor BAK1.The receptor-dependent immune response triggered by PpEli2 is able to protect various plant species against Phytophthora and fungal infections.Collectively,our work reveals the potential agricultural application of non-pathogenic elicitins and their receptors in conferring broad-spectrum resistance for plant protection. | Kun Yang Yi Wang Jialu Li Yaxin Du Ying Zhai Dong Liang Danyu Shen Rui Ji Xuexiang Ren Hao Peng Maofeng Jing Daolong Dou | 2023 | Horticulture Research2023，10，2： | 0 |
| 5 | Engineering crop Phytophthora resistance by targeting pathogen-derived PI3P for enhanced catabolism显示文摘Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR effectors.Here,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)P2.We fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant apoplast.Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P.sojae and P.infestans isolates,respectively.SP-PIP5K1 significantly reduced PI3P accumulation during P.sojae and soybean interaction.Knockout or inhibition of PI3 kinases(PI3Ks)in P.sojae compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P.Furthermore,we revealed that SP-PIP5K1 can interfere with the action of P.sojae mediated by the RxLR effector Avr1k.This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions. | Kun Yang Qiang Yan Yi Wang Wenyi Zhu Xiaodan Wang Xiaobo Li Hao Peng Yang Zhou Maofeng Jing Daolong Dou | 2023 | Plant Communications2023，4，2： | 0 |
| 6 | The warfare for plant highway:vascular plant–microbe interaction pinpoints lignin显示文摘Plant vascular pathogens are one kind of destructive pathogens in agricultural production.However,mechanisms behind the vascular pathogen-recognition and the subsequent defense responses of plants are not well known.A recent pioneering study on plant vascular immunity discovered a conserved MKP1-MPK-MYB signaling cascade that activates lignin biosynthesis in vascular tissues to confer vascular resistance in both monocot rice and the dicot Arabidopsis.The breakthrough provides a novel view on plant immunity to vascular pathogens and offers a potential strategy for the future breeding of disease-resistant crops. | Gan Ai Dong‑Lei Yang Daolong Dou | 2022 | Stress Biology2022，2，1： | 0 |
| 7 | Estimating the evolution of sea state non-Gaussianity based on a phase-resolving model显示文摘The occurrence of rogue waves is closely related to the non-Gaussianity of sea states,and this non-Gaussianity can be estimated using corresponding two-dimensional wave spectra.This paper presents an approach to non-Gaussianity estimation based on a phase-resolving model called the high-order spectral method(HOSM).Based on numerous HOSM simulations,a set of precalculated non-Gaussianity indicators was established that could be applied to real sea states without any calibration of spectral shapes.With a newly developed extraction approach,the indicators for given two-dimensional wave spectra could then be conveniently extracted from the precalculated dataset.The feasibility of the newly developed approach in a real wave environment is verified.Using the estimation approach,phase-resolved non-Gaussianity can now be illustrated throughout the evolution of sea states of interest,not just at a few specific times;and the level of non-Gaussianity at any time in a duration can be identified according to the statistics(e.g.,quantities)of the phase-resolved indicators,that are obtained throughout the duration concerned. | Xingjie JIANG Tingting ZHANG Dalu GAO Daolong WANG Yongzeng YANG | 2022 | Journal of Oceanology and Limnology2022，40，5： | 0 |
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