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| 1 | The N-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan Plasmodium falciparum mediates GT- Pase Rab2-dependent recruitment to membranes 显示文摘 | Daubenberger C A Tisdale E J Curcic M | 2003 | Biol Chem2003，384，： | 1 |
| 2 | Identification and re-combinant expression of glyceraldehydes-3-phosphate dehydrogenase of Plasmodium falciparum 显示文摘 | Daubenberger CA Pohl-Frank F Jiang G | 2000 | Gene2000，246，12： | 1 |
| 3 | Identification and characterization of heme-interacting proteins in the malaria parasite,Plasmodium falciparum显示文摘 | Campanale N Nickel C Daubenberger CA | 2003 | J Biol Chem2003，278，27： | 1 |
| 4 | Identification and recombinant expression of glyceraldehydes-3-phosphate dehydrogenase of Plasmodium falciparum显示文摘 | Daubenberger CA Poltl-Frank F Jiang G | 2000 | Gene2000，246，： | 1 |
| 5 | The N′-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan Plasmodium falciparum mediates GTPase Rab2-dependent recruitment to membranes显示文摘 | Daubenberger CA Tisdale EJ Curcic M | 2003 | Biol Chem2003，384，： | 1 |
| 6 | Sequence diversity of the merozoite surface protein 1 of Plasmodium falciparum in clinical isolates from The Kilombero ditrict,Tanzania显示文摘 | Jiang G Daubenberger C Huber W | | 0，，01： | 1 |
| 7 | Identification and recombinant expression of glyceraldehyde-3-phosphate dehydrogenase of Plasmodium falciparum显示文摘 | Daubenberger CA Pohl-Frank F Jiang G | 2000 | Gene2000，246，12： | 1 |
| 8 | Identification and recombinant expression of glyceraldehyde-3-phosphate dehydrogenase of Plasmodium falciparum显示文摘 | Daubenberger CA P(o)ltl-Frank F Jiang G | | 0，，1： | 1 |
| 9 | A rapid,single step purification method for immunogenic members of the hsp70 family:validation and application显示文摘 | Nandan D Daubenberge RC Mpimbaza G | 1994 | J Immunol Methods1994，176，2： | 1 |
| 10 | Caught in action:mechanistic insights into antibody-mediated inhibition of Plasmodium merozoite invasion显示文摘 | TREECK M TAMBORRINI M DAUBENBERGER C A | 2009 | Trends Parasitol2009，25，11： | 1 |
| 11 | The N'-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan Plasmodium falciparum mediates GTPase Rab2- dependent recruitment to membranes 显示文摘 | Daubenberger CA Tisdale EJ Curcic M | 2003 | Biol Chem2003，384，8： | 1 |
| 12 | TLR9 agonists as adjuvants for prophylactic and therapeutic vaccines 显示文摘 | DAUBENBERGER C A | 2007 | Curr Opin Mol Ther2007，，9： | 1 |
| 13 | Molecular characterization of a cognate 70 kDa heat shock protein of the protozoan Theileria parva 显示文摘 | Daubenberger C Heussler V Gobright E | 1997 | Mol Biochem Parasit1997，85，2： | 1 |
| 14 | Performance of a real-time PCR approach for diagnosing Schistosoma haematobium infections of different intensity in urine samples from Zanzibar显示文摘Background:Efforts to control and eliminate schistosomiasis have accelerated over the past decade.As parasite burden,associated morbidity and egg excretion decrease,diagnosis with standard parasitological methods becomes harder.We assessed the robustness and performance of a real-time PCR(qPCR)approach in comparison with urine filtration microscopy and reagent strip testing for the diagnosis of Schistosoma haematobium infections of different intensities.Methods:The robustness of DNA isolation and qPCR was validated in eight laboratories from Europe and Africa.Subsequently,792 urine samples collected during cross-sectional surveys of the Zanzibar Elimination of Schistosomiasis Transmission(ZEST)project in 2012-2017 were examined with qPCR in 2018.Diagnostic sensitivity of the qPCR was calculated at different infection intensity categories,using urine filtration microscopy as reference test.Spearman's rank correlation between Ct-values and S.haematobium egg counts was assessed and Ct-value percentiles for infection intensity categories determined.Results:S.haematobium Dra1 DNA-positive samples were identified correctly in all eight laboratories.Examination of urine samples from Zanzibar revealed Dra1 DNA in 26.8%(212/792)by qPCR,S.haematobium eggs in 13.3%(105/792)by urine filtration,and microhaematuria in 13.8%(109/792)by reagent strips.Sensitivity of the qPCR increased with augmenting egg counts:80.6%(29/36)for counts between 1 and 4 eggs,83.3%(15/18)for counts between 5 and 9 eggs,100%(23/23)for counts between 10 and 49 eggs,and 96.4%(27/28)for counts of 50+eggs.There was a significant negative correlation between Ct-values and egg counts(Spearman's rho=-0.49,P<0.001).Seventy-five percent of the Ct-values were≥33 in the egg-negative category,<31 in the light intensity category,and<24 in the heavy intensity category.Conclusions:While the sensitiivity of the qPCR was^80%for very light intensity infections(egg counts<10),in general,the Dra1 based qPCR assay detected twice as many S.haematobium infections compared with classical parasitological tests.The qPCR is hence a sensitive,urine-based approach for S.haernatcbium diagnosis that can be used for impact assessment of schistosomiasis elimination programmes,individual diagnosis,and in improved format also for verification and certification of elimination.Trial registration:ISRCTN,ISRCTN48837681.Registered 05 September 2012-Retrospectively registered. | Dominique Keller Julian Rothen Jean-Pierre Dangy Corina Saner Claudia Daubenberger Fiona Allan Shaali M.Ame Said M.Ali Fatma Kabole Jan Hattendorf David Rollinson Ralf Seyfarth Stefanie Knopp | 2020 | Infectious Diseases of Poverty2020，9，5： | 0 |
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