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| 1 | The role of gut microbiota(commensal bacteria)and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer:contribution of germ-free and gnotobiotic animal models of human diseases显示文摘Metagenomic approaches are currently being used to decipher the genome of the microbiota(microbiome),and,in parallel,functional studies are being performed to analyze the effects of the microbiota on the host.Gnotobiological methods are an indispensable tool for studying the consequences of bacterial colonization.Animals used as models of human diseases can be maintained in sterile conditions(isolators used for germ-free rearing)and specifically colonized with defined microbes(including non-cultivable commensal bacteria).The effects of the germ-free state or the effects of colonization on disease initiation and maintenance can be observed in these models.Using this approach we demonstrated direct involvement of components of the microbiota in chronic intestinal inflammation and development of colonic neoplasia(i.e.,using models of human inflammatory bowel disease and colorectal carcinoma).In contrast,a protective effect of microbiota colonization was demonstrated for the development of autoimmune diabetes in non-obese diabetic(NOD)mice.Interestingly,the development of atherosclerosis in germ-free apolipoprotein E(ApoE)-deficient mice fed by a standard low-cholesterol diet is accelerated compared with conventionally reared animals.Mucosal induction of tolerance to allergen Bet v1 was not influenced by the presence or absence of microbiota.Identification of components of the microbiota and elucidation of the molecular mechanisms of their action in inducing pathological changes or exerting beneficial,disease-protective activities could aid in our ability to influence the composition of the microbiota and to find bacterial strains and components(e.g.,probiotics and prebiotics)whose administration may aid in disease prevention and treatment. | Helena Tlaskalova-Hogenova Renata Stepankova Hana Kozakova Tomas Hudcovic Luca Vannucci Ludmila Tuckova Pavel Rossmann TomasHrncır Miloslav Kverka Zuzana Zakostelska Klara Klimesova Jaroslava Pribylova Jirina Bartova Daniel Sanchez Petra Fundova Dana Borovska Dagmar Sru˚tkova Zdenek Zıdek Martin Schwarzer Pavel Drastich David P Funda | 2011 | Cellular & Molecular Immunology2011，8，2： | 47 |
| 2 | Quasispecies dynamics in main core epitopes of hepatitis B virus by ultra-deep-pyrosequencing显示文摘AIM:To investigate the variability of the main immunodominant motifs of hepatitis B virus(HBV) core gene by ultra-deep-pyrosequencing(UDPS).METHODS:Four samples(2 genotype A and 2 genotype D) from 4 treatment-na ve patients were assessed for baseline variability.Two additional samples from one patient(patient 4,genotype D) were selected for analysis:one sample corresponded to a 36-mo treatment-free period from baseline and the other to the time of viral breakthrough after 18 mo of lamivudine treatment.The HBV region analyzed covered amino acids 40 to 95 of the core gene,and included the two main epitopic regions,Th50-69 and B74-84.UDPS was carried out in the Genome Sequencer FLX system(454 Life Sciences,Roche).After computer filtering of UDPS data based on a Poisson statistical model,122 813 sequences were analyzed.The most conserved position detected by UDPS was analyzed by site-directed mutagenesis and evaluated in cell culture.RESULTS:Positions with highest variability rates were mainly located in the main core epitopes,confirming their role as immune-stimulating regions.In addition,the distribution of variability showed a relationship with HBV genotype.Patient 1(genotype A) presented the lowest variability rates and patient 2(genotype A) had 3 codons with variability higher than 1%.Patient 3 and 4(both genotype D) presented 5 and 8 codons with variability higher than 1%,respectively.The median baseline frequencies showed that genotype A samples had higher variability in epitopic positions than in the other positions analyzed,approaching significance(P = 0.07,sample 1 and P = 0.05,sample 2).In contrast,there were no significant differences in variability between the epitopic and other positions in genotype D cases.Interestingly,patient 1 presented a completely mutated motif from amino acid 64 to 67(E 64 LMT 67),which is commonly recognized by T helper cells.Additionally,the variability observed in all 4 patients was particularly associated with the E 64 LMT 67 motif.Codons 78 and 79 were highly conserved in all samples,in keeping with their involvement in the interaction between the HBV virion capsid and the surface antigens(HBsAg).Of note,codon 76 was even more conserved than codons 78 and 79,suggesting a possible role in HBsAg interactions or even in hepatitis B e antigen conformation.Sequential analysis of samples from patient 4(genotype D) illustrated the dynamism of the HBV quasispecies,with strong selection of one minor baseline variant coinciding with a decrease in core variability during the treatment-free and lamivudinetreated period.The drop in variability seemed to result from a 'steady state' situation of the HBV quasispecies after selection of the variant with greatest fitness.CONCLUSION:Host immune pressure seems to be the main cause of HBV core evolution.UDPS analysis is a useful technique for studying viral quasispecies. | Maria Homs Maria Buti David Tabernero Josep Quer Alex Sanchez Noelia Corral Rafael Esteban Francisco Rodriguez-Frias | 2012 | World Journal of Gastroenterology2012，18，42： | 5 |
| 3 | Colorectal cancer screening and surveillance in patients with inflammatory bowel disease in 2021显示文摘The detection of dysplasia in patients with inflammatory bowel disease(IBD)continues to be important given the increased risk of colorectal cancer in this population.Therefore,in 2017,we performed a review and update of the recommendations for the management and follow-up of patients with IBD based on the clinical practice guidelines of various scientific societies.The present manuscript focuses on new aspects of the detection,follow-up,and management of dysplasia according to the latest studies and recommendations.While chromoendoscopy with targeted biopsy continues to be the technique of choice for the screening and detection of dysplasia in IBD,the associated difficulties mean that it is now being compared with other techniques(virtual chromoendoscopy),which yield similar results with less technical difficulties.Furthermore,the emergence of new endoscopy techniques that are still being researched but seem promising(e.g.,confocal laser endomicroscopy and full-spectrum endoscopy),together with the development of devices that improve endoscopic visualization(e.g.,Endocuff Vision),lead us to believe that these approaches can revolutionize the screening and follow-up of dysplasia in patients with IBD.Nevertheless,further studies are warranted to define the optimal follow-up strategy in this patient population. | Jose Maria Huguet Luis Ferrer-Barceló Patrícia Suárez Eva Sanchez Jose David Prieto Victor Garcia Javier Sempere | 2022 | World Journal of Gastroenterology2022，28，5： | 2 |
| 4 | Projected effectiveness of HIV detection during early infection and rapid ART initiation among MSM and transgender women in Peru: A modeling study显示文摘Background:The Sabes study,a treatment as prevention intervention in Peru,tested the hypothesis that initiating antiretroviral therapy(ART)early in HIV infection when viral load is high,would markedly reduce onward HIV transmission among high-risk men who have sex with men(MSM)and transgender women(TW).We investigated the potential population-level benefits of detection of HIV early after acquisition and rapid initiation of ART.Methods:We designed a transmission dynamic model to simulate the HIV epidemic among MSM and TW in Peru,calibrated to data on HIV prevalence and ART coverage from 2004 to 2011.We assessed the impact of an intervention starting in 2018 in which up to 50%of the new infections were diagnosed within three months of acquisition and initiated on ART within 1 month of diagnosis.We estimated the impact of the intervention over 20 years using the cumulative prevented fraction of new HIV infections compared to scenarios without intervention.Findings:Our model suggests that only 19%of the infected MSM and TW are virally suppressed in 2018 and 35%e40%of the new HIV infections are transmitted from contacts with acutely-infected partners.An intervention reaching 10%of all acutely infected MSM and TW is projected to prevent 13.3%[Uncertainty interval:11.9%e14.3%]of the new infections over 20 years and reduce HIV incidence in 2038 by 24%.Reaching 50%of all acutely infected MSM and TWwill increase the prevalence of viral suppression in 2038 to 59%and prevent 41%of expected infections over 20 years.Reaching 50%of the high-risk MSM and TW in acute phase would reduce HIV incidence in 2038 by 60%and prevent 36%of new infections between 2018 and 2038.Conclusions:Early detection of HIV infections and rapid initiation of ART among MSM is desirable as it would increase the effectiveness of the HIV prevention program in Peru.Targeting high-risk MSM and TW will be highly efficient. | Dobromir Dimitrov Daniel Wood Angela Ulrich David A.Swan Blythe Adamson Javier R.Lama Jorge Sanchez Ann Duerr | 2019 | Infectious Disease Modelling2019，4，1： | 2 |
| 5 | Updated Clinical Classification of Pulmonary Hypertension显示文摘 | Gerald Simonneau Michael A. Gatzoulis Ian Adatia David Celermajer Chris Denton Ardeschir Ghofrani Miguel Angel Gomez Sanchez R. Krishna Kumar Michael Landzberg Roberto F. Machado Horst Olschewski Ivan M. Robbins Rogiero Souza | 2013 | Journal of the American College of Cardiology2013，，25： | 2 |
| 6 | Investigating the Synthesis of RBF Networks显示文摘InvestigatingtheSynthesisofRBFNetworks￥V.DavidSanchezA.(GermanAerospaceResearchEstablishment,DLROberpfaffenhofenInstituteforR... | V. David Sanchez A.(German Aerospace Research Establishment, DLR OberpfaffenhofenInstitute for Robottes and System DynamicsD-82230 Wessling, Germany) | 1996 | Journal of Systems Engineering and Electronics1996，7，3： | 2 |
| 7 | Advanced support vector machines and kernel methods显示文摘 | David V Sanchez A | 2003 | Neurocomputing2003，55，： | 1 |
| 8 | Advanced Support Vector Machines and Kernel Methods显示文摘 | David V Sanchez A | 2003 | Neurocomputing2003，55，： | 1 |
| 9 | An empiri- cal study on the impact of standardization of materials and purchasing procedures on purchasing and business perfor- mance显示文摘 | Cristobal Sanchez Rodriguez David Hems worth | 2006 | Supply Chain Management2006，11，1： | 1 |
| 10 | Serial measurement of N-terminal pro–B-type natriuretic peptide and cardiac troponin T for cardiovascular disease risk assessment in the Multi-Ethnic Study of Atherosclerosis (MESA)显示文摘 | Lori B. Daniels Paul Clopton Christopher R. deFilippi Otto A. Sanchez Hossein Bahrami Joao A.C. Lima Russell P. Tracy David Siscovick Alain G. Bertoni Philip Greenland Mary Cushman Alan S. Maisel Michael H. Criqui | 2015 | American Heart Journal2015，，6： | 1 |
| 11 | On the number and distribution of RBF centers显示文摘 | David Sanchez A | 1995 | Neurocomputing1995，，7： | 1 |
| 12 | Neuropsychological functioning in a subclinical obsessive-compulsive sample显示文摘 | David Mataix Cols Carme Junque Miquel Sanchez Turett | 1999 | Biol Psychiatry1999，45，7： | 1 |
| 13 | Advanced support vector machines and kernel methods显示文摘 | David V Sanchez A | 2003 | Neuro computing2003，55，12： | 1 |
| 14 | Ontolo- gy-based semantic similarity a new feature-based approach显示文摘 | SANCHEZ D MONTSERRAT B DAVID I | 2012 | Expert Systems with Applications2012，39，9： | 1 |
| 15 | Enabling semantic similarity estimation across multiple ontologies: An evaluation in the biomedical domain 显示文摘 | David Sanchez Albert Sole-Ribalta Montserrat Batet | 2012 | Journal of Biomedical Informatics2012，45，9： | 1 |
| 16 | Cardiac pacing in balloon aortic valvuloplasty显示文摘 | David F Sanchez A Yanez L | 2007 | Int J Cardiol2007，116，3： | 1 |
| 17 | Advanced support vector machines and kernel method显示文摘 | Sanchez David V | 2003 | Neurocomputing2003，55，： | 1 |
| 18 | Advanced support vector machines andkernel methods显示文摘 | David V Sanchez A | 2003 | Neurocomputing2003，55，： | 1 |
| 19 | Crypt Dysplasia With Surface Maturation: A Clinical, Pathologic, and Molecular Study of a Barrett?s Esophagus Cohort显示文摘 | Leslie C. Lomo Patricia L. Blount Carissa A. Sanchez X. Li Patricia C. Galipeau David S. Cowan Kamran Ayub Peter S. Rabinovitch Brian J. Reid Robert D. Odze | 2006 | The American Journal of Surgical Pathology2006，，： | 1 |
| 20 | Advanced support vector machines and kernel methods显示文摘 | David Sanchez A V | 2003 | Neuro Computing2003，55，： | 1 |
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