7篇
您的检索式：作者名="David Shafer"
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| 1 | Measuring the predictive performance of computer-controlled infusion pumps显示文摘 | John R. Varvel David L. Donoho Steven L. Shafer | 1992 | Journal of Pharmacokinetics and Biopharmaceutics1992，，1： | 1 |
| 2 | Influence of Age and Gender on the Pharmacokinetics and Pharmacodynamics of Remifentanil: I. Model Development显示文摘 | Charles F. Minto Thomas W. Schnider Talmage D. Egan Elizabeth Youngs Harry J. M. Lemmens Pedro L. Gambus Valerie Billard John F. Hoke Katherine H. P. Moore David J. Hermann Keith T. Muir Jaap W. Mandema Steven L. Shafer | 1997 | Anesthesiology1997，，： | 1 |
| 3 | Measuring the predictive performance of computer-controlled infusion pumps显示文摘 | John R. Varvel David L. Donoho Steven L. Shafer | 1992 | Journal of Pharmacokinetics and Biopharmaceutics1992，，1： | 1 |
| 4 | The Impact of Open Reduction Internal Fixation on Acute Pain Management in Unstable Pelvic Ring Injuries显示文摘 | David P. Barei Brian L. Shafer Daphne M. Beingessner Michael J. Gardner Sean E. Nork M. L. Chip Routt | 2010 | The Journal of Trauma: Injury, Infection, and Critical Care2010，，4： | 1 |
| 5 | Seven Novel Probe Systems for Real-Time PCR Provide Absolute Single-Base Discrimination, Higher Signaling, and Generic Components显示文摘 | James L. Murray Peixu Hu David A. Shafer | 2014 | The Journal of Molecular Diagnostics2014，，6： | 1 |
| 6 | Optical design with only two surfaces显示文摘 | David Shafer | 1980 | SPIE1980，237，： | 1 |
| 7 | Point mutation in regulatory region of msrA gene contributes to the telithromycin resistance induced by erythromycin in Staphylococcus aureus显示文摘msrA encodes an efflux protein in Staphylococcus spp.that confers inducible resistance to macrolides,such as erythromycin(ERY),but not telithromycin(TEL).Thus,msrA+S.aureus remain susceptible to TEL.Production of msrAis regulated by a 320-bp upstream region,presumably by translational attenuation,similar to regulation of erm.We investigated whether ERY could induce TEL resistance in msrA+S.aureus.MICs of ERY and TEL were determined by broth microdilution(BMD).Inducible TEL resistance was detected by a BMD checkerboard panel containing combinations of ERY and TEL and by a D-test where an ERY disk was placed 15mm from a TEL disk on a blood agar plate.Blunting of the TEL zones of inhibition proximal to the ERY disk indicated inducible resistance and no blunting indicated no inducible resistance.Approximately 400bp of the upstream regulatory region and 200bp of msrA were amplified by PCR and sequenced.The TEL MICs for 10 msrA+isolates ranged from 0.06-2g/mL(MIC90=0.25g/mL);ERY MICs were all≥32g/mL.All isolates showed an increase in TEL MICs of>2doubling dilutions(MIC90>4g/mL)in the presence of ERY;All were also positive by D-test.For 9isolates(initial TEL MIC<1g/mL),constitutive TEL resistance was selected by plating cells on Mueller-Hinton agar with 2g/mL TEL.All 9isolates grew on the selective media and were resistant to TEL(MIC ranged 2-8g/mL).Mutation to constitutive resistance occurred at a rate of 8×10-7 to 2.4×10-6.Eight of the 9resistant isolates had a single nucleotide substitution in the upstream regulatory region relative to the parent strain.Specifically,six isolates had a G to T mutation at position-222;one had a C to A mutation at-228;And one had a C to A mutation at-247.Transformation of the msrAgene with above point mutation in regulatory region into S.aureus RN4220conferred the strain resistant(MIC=8g/mL)to the telithromycin while transformants with the wild-type msrAgene remained susceptible(MIC=1g/mL)to telithromycin. | David Shafer Xiao Shali Xia Han Wang Jue Jason Loft Zhang Weidong Fu Weiling | 2013 | 国际检验医学杂志2013，34，21： | 0 |
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