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| 1 | Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.)显示文摘A narrow leaf mutant was obtained after T-DNA transformation conducted on a rice variety Zhonghua 11. Several abnormal morphological characteristics, including semi-dwarf, delayed flowering time, narrow and inward rolling leaves, and lower seed-setting, were observed. The rate of net photosynthesis (un-der saturate light) of flag leaves in the mutant was significantly lower than that of the wild type. More-over, the leaf transpiration rate and stomatal conductance in the mutant flag leaf were lower than those of the wild type at the grain filling stage. It was found that the mutant phenotype was not caused by the T-DNA insertion. Genetic analysis showed that the mutant was controlled by a single recessive gene, designated as nal3(t). A genetic linkage map was constructed using a large F2 mapping population de-rived from a cross between nal3(t) and an indica variety Longtefu B with 6 polymorphic markers on chromosome 12 identified from 366 SSR markers by the BAS method. Gene nal3(t) was mapped be-tween the markers RM7018 and RM3331. Fine mapping of nal3(t) locus was conducted with 22 newly developed STS markers based on the sequence diversity around the region harboring nal3(t) between Nipponbare and 93-11, and nal3(t) was finally mapped to a 136-kb region between the STS markers NS10 and RH12-8. | WANG DeKai LIU HeQin LI KeLei LI SuJuan TAO YueZhi | 2009 | Chinese Science Bulletin2009，54，5： | 19 |
| 2 | The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice显示文摘Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice.WG1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell proliferation.Interestingly,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor.In contrary,OsbZIP47 restricts grain growth by decreasing cell proliferation.Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation.Genetic analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain growth.Taken together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops. | Jianqin Hao Dekai Wang Yingbao Wu Ke Huang Penggen Duan Na Li Ran Xu Dali Zeng Guojun Dong Baolan Zhang Limin Zhang Dirk Inze Qian Qian Yunhai Li | 2021 | Molecular Plant2021，14，8： | 14 |
| 3 | The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells Superior effects over original formula of Buyang Huanwu decoction显示文摘The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction (active principle region of decoction for invigorating yang for recuperation). After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula. | Jinghui Zheng Yi Wan Jianhuai Chi Dekai Shen Tingting Wu Weimin Li Pengcheng Du | 2012 | Neural Regeneration Research2012，7，4： | 9 |
| 4 | Novel trichromatic phosphor Co-doped with Eu, Tb in SiO_2 gel matrix显示文摘The Eu, Tb co-doped SiO2 matrix tricolor fluorescence system was prepared by sol-gel technique. Red emission at 618 nm, green emission at 543 nm and blue emission at 350―500 nm were observed in the PL spectra of the sample, indicating that Eu3+, Eu2+ and Tb3+ ions coexisted in the matrix. In the co-doped sample, the blue emission of Eu2+ was much stronger than that of the sample single doped with Eu, which implied that the electron transfer between Eu3+ and Tb3+ maybe happened in the SiO2 matrix. The influences of the annealing temperature and Tb concentration on the PL spectra of the samples were investigated. The optimal doped concentration of Tb was determined to be 0.2% and the optimal annealing temperature 850℃. Annealed at 600℃, Tb3+ had a sensitizing effect on Eu3+ in the SiO2 matrix, and the emission intensity of Eu3+ in the Eu, Tb co-doped sample was more than four times that of the single doped sample, which could be attributed to the energy transfer from Tb3+ to Eu3+. | HU XiaoYun FAN Jun LI Ting ZHANG DeKai BAI Jinmao REN ZhaoYu HOU Xun | 2007 | Chinese Science Bulletin2007，52，4： | 5 |
| 5 | Electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in the injured spinal cord of adult rats显示文摘A contusive model of spinal cord injury at spinal segment T8-9 was established in rats. Huantiao (GB30) and Huatuojiaji (Ex-B05) were punctured with needles, and endogenous neural stem cells were labeled with 5-bromo-2'-deoxyuridine (BrdU) and NG2. Double immunofluorescence staining showed that electroacupuncture markedly increased the numbers of BrdU+/NG2+ cells at spinal cord tissue 15 mm away from the injury center in the rostral and caudal directions. The results suggest that electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in rats with spinal cord injury. | Haiying Wu Min HU Dekai Yuan Yunhui Wang Jing Wang Tao Li Chuanyun Qian Hualin YU | 2012 | Neural Regeneration Research2012，7，15： | 4 |
| 6 | Enhanced Light-Harvesting Efficiency and Adaptation: A Review on Visible-Light-Driven Micro/Nanomotors显示文摘As visible light accounts for a larger proportion of solar energy and is harmless to living organisms,it has the potential to be the energy source of micro/nanomotors,which transform visible-light energy into mechanical motion,for different applications,especially in environmental remediation.However,how to precisely control the motion of visible-light-driven micro/nanomotors(VLD-MNMs)and efficiently utilize the weak visible-light photon energy to acquire rapid motion are significant challenges.This review summarizes the most critical aspects,involving photoactive materials,propulsion mechanisms,control methods,and applications of VLD-MNMs,and discusses strategies to systematically enhance the energyharvesting efficiency and adaptation.At first,the photoactive materials have been divided into inorganic and organic photoactive materials and comprehensively discussed.Then,different propulsion mechanisms of the current VLD-MNMs are presented to explain the improvement in the actuation force,speed,and environmental adaptability.In addition,considering the characteristics of easy control of VLD-MNMs,we summarized the direction,speed,and cluster control methods of VLD-MNMs for different application requirements.Subsequently,the potential applications of VLD-MNMs,e.g.,in environmental remediation,micropumps,cargo delivery,and sensing in microscale,are presented.Finally,discussions and suggestions for future directions to enhance the energy-harvesting efficiency and adaptation of VLD-MNMs are provided. | Dekai Zhou Rencheng Zhuang Xiaocong Chang Longqiu Li | 2020 | Research2020，，1： | 3 |
| 7 | Characterization and molecular cloning of a serine hydroxymethyltransferase 1(OsSHM_1) in rice显示文摘Serine hydroxymethyltransferase(SHMT) is important for one carbon metabolism and photorespiration in higher plants for its participation in plant growth and development,and resistance to biotic and abiotic stresses. A rice serine hydroxymethyltransferase gene, Os SHM1, an ortholog of Arabidopsis SHM1, was isolated using map-based cloning. The osshm1 mutant had chlorotic lesions and a considerably smaller,lethal phenotype under natural ambient CO2 concentrations,but could be restored to wild type with normal growth under elevated CO2levels(0.5% CO2), showing a typical photorespiratory phenotype. The data from antioxidant enzymes activity measurement suggested that osshm1 was subjected to signi fi cant oxidative stress. Also, Os SHM1 was expressed in allorgans tested(root, culm, leaf, and young panicle) but predominantly in leaves. Os SHM1 protein is localized to the mitochondria. Our study suggested that molecular function of the Os SHM1 gene is conserved in rice and Arabidopsis. | Dekai Wang Heqin Liu Sujuan Li Guowei Zhai Jianfeng Shao Yuezhi Tao | 2015 | Journal of Integrative Plant Biology2015，57，9： | 3 |
| 8 | Epigenetic considerations and the clinical reevaluation of the overlap syndrome between primary biliary cirrhosis and autoimmune hepatitis显示文摘 | Qixia Wang Carlo Selmi Xinmin Zhou Dekai Qiu Zenshan Li Qi Miao Xiaoyu Chen Jianhong Wang Edward L. Krawitt M. Eric Gershwin Ying Han Xiong Ma | 2012 | Journal of Autoimmunity2012，，： | 2 |
| 9 | The critical role of myeloid-derived suppressor cells and FXR activation in immune-mediated liver injury显示文摘 | Haiyan Zhang Yuan Liu Zhaolian Bian Shanshan Huang Xiaofeng Han Zhengrui You Qixia Wang Dekai Qiu Qi Miao Yanshen Peng Xiaoying Li Pietro Invernizzi Xiong Ma | 2014 | Journal of Autoimmunity2014，，： | 1 |
| 10 | Magnetic Nanomotor-Based Maneuverable SERS Probe显示文摘Surface-enhanced Raman spectroscopy(SERS)is a powerful sensing technique capable of capturing ultrasensitive fingerprint signal of analytes with extremely low concentration.However,conventional SERS probes are passive nanoparticles which are usually massively applied for biochemical sensing,lacking controllability and adaptability for precise and targeted sensing at a small scale.Herein,we report a“rod-like”magnetic nanomotor-based SERS probe(MNM-SP)that integrates a mobile and controllable platform of micro-/nanomotors with a SERS sensing technique.The“rod-like”structure is prepared by coating a thin layer of silica onto the self-assembled magnetic nanoparticles.Afterwards,SERS hotspots of silver nanoparticles(AgNPs)are decorated as detecting nanoprobes.The MNM-SPs can be navigated on-demand to avoid obstacles and target sensing sites by the guidance of an external gradient magnetic field.Through applying a rotating magnetic field,the MNM-SPs can actively rotate to efficiently stir and mix surrounding fluid and thus contact with analytes quickly for SERS sensing.Innovatively,we demonstrate the self-cleaning capability of the MNM-SPs which can be used to overcome the contamination problem of traditional single-use SERS probes.Furthermore,the MNM-SPs could precisely approach the targeted single cell and then enter into the cell by endocytosis.It is worth mentioning that by the effective mixing of intracellular biocomponents,much more informative Raman signals with improved signal-to-noise ratio can be captured after active rotation.Therefore,the demonstrated magnetically activated MNM-SPs that are endowed with SERS sensing capability pave way to the future development of smart sensing probes with maneuverability for biochemical analysis at the micro-/nanoscale. | Yong Wang Yuhuan Liu Yang Li Dandan Xu Xi Pan Yuduo Chen Dekai Zhou Bo Wang Huanhuan Feng Xing Ma | 2020 | Research2020，，1： | 1 |
| 11 | Puzzle and Innovation: Thoughts over New Countryside Planning in New Era | YE Bin, WANG Yaonan, ZHENG Xiaohua, TAO Dekai LI Caige | 2010 | China City Planning Review2010，19，4： | 0 |
| 12 | An electrochemical study on the effects of marine fouling organisms on steel used in the sea显示文摘-Study on the effects of microorganism secretive membrane and covering layer of macrofouling organisms-Balanus reticulatus, Ostrea plicatula, Membranipora, Corophium and Algae on the corrosion of carbon steel and low-alloy steel by the determination of natural corrosive potentials, linear polarization and the polarization curver of dynamic potential scanning through an electrochemical equipment.The results show that the microorganism secretion membrane formed on sample surface has some protective role, during the initial period of exposure (about ten days). Then protective role decreases and corrosive rate increases with the lapse of immersion time. The protective role of some macrofouling organisms is shown in order as follows: Corophium> Balanus reticulatus>Algae>Membranipora>Ostrea plicatula. | Hong Xuebao,, Lin Ruimu, Li Dekai and Zheng Jiancheng(Receired February 6. 1990, accepted September 8. 1990) | 1991 | Acta Oceanologica Sinica1991，10，2： | 0 |
| 13 | 3D printing of high-precision and ferromagnetic functional devices显示文摘The development of projection-based stereolithography additive manufacturing techniques and magnetic photosensitive resins has provided a powerful approach to fabricate miniaturized magnetic functional devices with complex three-dimensional spatial structures.However,the present magnetic photosensitive resins face great challenges in the trade-off between high ferromagnetism and excellent printing quality.To address these challenges,we develop a novel NdFeB-Fe_(3)O_(4) magnetic photosensitive resin comprising 20 wt.%solid loading of magnetic particles,which can be used to fabricate high-precision and ferromagnetic functional devices via micro-continuous liquid interface production process.This resin combining ferromagnetic NdFeB microparticles and strongly absorbing Fe_(3)O_(4) nanoparticles is able to provide ferromagnetic capabilities and excellent printing quality simultaneously compared to both existing soft and hard magnetic photosensitive resins.The established penetration depth model reveals the effect of particle size,solid loading,and absorbance on the curing characteristics of magnetic photosensitive resin.A high-precision forming and ferromagnetic capability of the NdFeB-Fe_(3)O_(4) magnetic photosensitive resin are comprehensively demonstrated.It is found that the photosensitive resin(NdFeB:Fe_(3)O_(4)=1:1)can print samples with sub-40μm fine features,reduced by 87%compared to existing hard magnetic photosensitive resin,and exhibits significantly enhanced coercivity and remanence in comparison with existing soft magnetic photosensitive resins,showing by an increase of 24 times and 6 times,respectively.The reported NdFeB-Fe_(3)O_(4) magnetic photosensitive resin is anticipated to provide a new functional material for the design and manufacture of next-generation micro-robotics,electromagnetic sensor,and magneto-thermal devices. | Zhiyuan Huang Guangbin Shao Dekai Zhou Xinghong Deng Jing Qiao Longqiu Li | 2023 | International Journal of Extreme Manufacturing2023，5，3： | 0 |
| 14 | A Bioinspired Soft Robot Combining the Growth Adaptability of Vine Plants with a Coordinated Control System显示文摘Tip-extending soft robots,taking flexible film or rubber as body material and fluid pressure as input power,exhibit excellent advantages in constrained and cluttered environments for detection and manipulation.However,existing soft continuum robots are of great challenges in achieving multiple,mutually independent,and on-demand active steering over a long distance without precise steering control.In this paper,we introduce a vine-like soft robot made up of a pressurized thin-walled vessel integrated with the high controllability of a control system with multiple degrees of freedom in three dimensions.Moreover,steering and kinematic models to relate the steering angle and robot length to the location of the robot tip are provided,and a dynamic finite element model for analyzing the motion of the spatial consecutive steering is established.We demonstrate the abilities of disinfection of the robot moving in a long and tortuous pipeline and detection in a multi-obstacle constrained environment.It is established that the robot exhibits great advantages in active consecutive steering over a long distance,high controllability in completing more complex path planning,and significant ability of carrying operational tools for ventilation pipeline disinfection and multi-obstacle detection.The bionic soft robot shows great promise for use in environment sensing,target detecting,and equipment servicing. | Pengchun Li Yongchang Zhang Guangyu Zhang Dekai Zhou Longqiu Li | 2022 | Research2022，，1： | 0 |
| 15 | Potential application of functional micro-nano structures in petroleum显示文摘This paper takes micro-nano motors and metamaterials as examples to introduce the basic concept and development of functional micro nano structures, and analyzes the application potential of the micro-nano structure design and manufacturing technology in the petroleum industry. The functional micro-nano structure is the structure and device with special functions prepared to achieve a specific goal. New functional micro-nano structures are classified into mobile type(e.g. micro-nano motors) and fixed type(e.g. metamaterials), and 3 D printing technology is a developed method of manufacturing. Combining the demand for exploration and development in oil and gas fields and the research status of intelligent micro-nano structures, we believe that there are 3 potential application directions:(1) The intelligent micro-nano structures represented by metamaterials and smart coatings can be applied to the oil recovery engineering technology and equipment to improve the stability and reliability of petroleum equipment.(2) The smart micro-nano robots represented by micro-motors and smart microspheres can be applied to the development of new materials for enhanced oil recovery, effectively improving the development efficiency of heavy oil, shale oil and other resources.(3) The intelligent structure manufacturing technology represented by 3 D printing technology can be applied to the field of microfluidics in reservoir fluids to guide the selection of mine flooding agents and improve the efficiency of mining. | LIU He JIN Xu ZHOU Dekai YANG Qinghai LI Longqiu | 2018 | Petroleum Exploration and Development2018，45，4： | 0 |
| 16 | Robust Watermarking Algorithm for Medical Volume Data Based on Polar Cosine Transform and 3D-DCT显示文摘The amount of 3D data stored and transmitted in the Internet of Medical Things(IoMT)is increasing,making protecting these medical data increasingly prominent.However,there are relatively few researches on 3D data watermarking.Moreover,due to the particularity of medical data,strict data quality should be considered while protecting data security.To solve the problem,in the field of medical volume data,we proposed a robust watermarking algorithm based on Polar Cosine Transform and 3D-Discrete Cosine Transform(PCT and 3D-DCT).Each slice of the volume data was transformed by PCT to obtain feature row vector,and then the reshaped three-dimensional feature matrix was transformed by 3D-DCT.Based on the contour information of the volume data and the detail information of the inner slice,the visual feature vector was obtained by applying the per-ceptual hash.In addition,the watermark was encrypted by a multi-sensitive initial value Sine and Piecewise linear chaotic Mapping(SPM)system,and embedded as a zero watermark.The key was stored in a third party.Under the same experimental conditions,when the volume data is rotated by 80 degrees,cut 25%along the Z axis,and the JPEG compression quality is 1%,the Normalized Correlation Coefficient(NC)of the extracted watermark is 0.80,0.89,and 1.00 respectively,which are significantly higher than the comparison algorithm. | Pengju Zhang Jingbing Li Uzair Aslam Bhatti Jing Liu Yen-wei Chen Dekai Li Lei Cao | 2023 | Computers, Materials & Continua2023，，6： | 0 |
| 17 | LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice显示文摘Heading date is one of the most important agronomic traits of rice,which critically affects rice ecogeographical adaptation,yield and quality.In this study,a late heading date 3(lhd3)mutant was screened from the^(60)Co-γirradiation mutant library.The lhd3 delayed heading date in rice under both short day and long day conditions.Map-based cloning combined with Mutmap strategy was adopted to isolate the causal LHD3 gene.The LHD3 gene encodes a DNA_J domain protein,which was ubiquitously expressed in various plant organs,and dominant expressed in stems and leaves.Subcellular localization analysis showed that LHD3 was localized to nucleus,indicating that LHD3 may interact with other elements to regulate the expression of flowering genes.The transcriptions of the heading activators Ehd1,Hd3a and RFT1 significantly decreased in the lhd3 mutant,suggesting that LHD3 may control the heading date through the Ehd1-Hd3a/RFT1 photoperiodic flowering pathway.The variation and haplotype analyses of the genomic region of LHD3 showed that there were 7 haplotypes in the LHD3 region from 4702 accessions.The haplotypes of LHD3 can be divided into two classes:class a and class b,and the heading dates of these two classes were significantly different.Further study showed that two single nucleotide polymorphisms(SNPs),SNP10(G2100C)in Hap II and SNP3(C861T)in Hap VII,may be the functional sites causing early and late heading in accessions.Nucleotide diversity analysis showed LHD3 had been selected in the indica population,rather than in the japonica population.Therefore,the present study sheds light on the regulation of LHD3 on heading date in rice and suggests that LHD3 is a novel promising new target for rice molecular design and breeding improvement. | LIU Qiao QIU Linlin HUA Yangguang LI Jing PANG Bo ZHAI Yufeng WANG Dekai | 2023 | Rice science2023，30，5： | 0 |
| 18 | A Bioinspired Soft Robot Combining the Growth Adaptability of Vine Plants with a Coordinated Control System显示文摘Tip-extending soft robots,taking flexible film or rubber as body material and fluid pressure as input power,exhibit excellent advantages in constrained and cluttered environments for detection and manipulation.However,existing soft continuum robots are of great challenges in achieving multiple,mutually independent,and on-demand active steering over a long distance without precise steering control.In this paper,we introduce a vine-like soft robot made up of a pressurized thin-walled vessel integrated with the high controllability of a control system with multiple degrees of freedom in three dimensions.Moreover,steering and kinematic models to relate the steering angle and robot length to the location of the robot tip are provided,and a dynamic finite element model for analyzing the motion of the spatial consecutive steering is established.We demonstrate the abilities of disinfection of the robot moving in a long and tortuous pipeline and detection in a multi-obstacle constrained environment.It is established that the robot exhibits great advantages in active consecutive steering over a long distance,high controllability in completing more complex path planning,and significant ability of carrying operational tools for ventilation pipeline disinfection and multi-obstacle detection.The bionic soft robot shows great promise for use in environment sensing,target detecting,and equipment servicing. | Pengchun Li Yongchang Zhang Guangyu Zhang Dekai Zhou Longqiu Li | 2021 | Research2021，，1： | 0 |
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