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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Insulin resistance impairs sustained response rate to peginterferon plus ribavirin in chronic hepatitis C patients显示文摘 | Manuel Romero-Gómez Maria Del Mar Viloria Raúl J. Andrade Javier Salmerón Moisés Diago Conrado M. Fernández-Rodríguez Raquel Corpas Marina Cruz Lourdes Grande Luis Vázquez Paloma Mu?oz-de-Rueda Pilar López-Serrano Ana Gila María L. Gutiérrez Celia Pérez A | 2005 | Gastroenterology2005，，3： | 2 |
| 3 | Neurogenic aspects of inflammation显示文摘 | Schaible HG Del RA Matucci-Cerinic M | 2005 | Rheum Dis Clin North Am2005，31，1： | 1 |
| 4 | Sinus floor augmentation with simultaneous implant placement using Choukroun's platelet-rich fibrin as the sole grafting mate- rial:a radiologic and histologic study at 6 months显示文摘 | Mazor Z Horowitz RA Del Corso M | 2009 | J Periodontol2009，80，12： | 1 |
| 5 | Chemical and electrochemical study of fabrics coated with reduced graphene oxide 显示文摘 | Molina J' Fernandez J del RA | 2013 | AppliedSurface Science2013，279，： | 1 |
| 6 | Histiocytic sarcoma: a case report and review of the literature 显示文摘 | Khosravi SP del Castillo RA | 2005 | An Med Interna2005，22，4： | 1 |
| 7 | Estrogen attenuates glutamate induced cell death by inhibiting Ca^2+ infux through L-type voltage-gated Ca^2+ channels 显示文摘 | Sribnick EA Del RA Ray SK | 2009 | Brain Res2009，1276，： | 1 |
| 8 | Sorafenib in combination with capecitabine: an oral regimen for patients with HER2-negative locally advanced or metastatic breast cancer 显示文摘 | Baselga J Segalla JG Roch6 H Del Giglio A Pinczowski H Ciruelos EM Filho SC G6mez P Van Eyll B Bermejo B Llombart A Garicochea B Durdn Md Hoff PM Espi6 M de Moraes AA Ribeiro RA Mathias C Gil Gil M Ojeda B Morales J Kwon Ro S Li S Costa F | 2012 | J Clin Oncol2012，30，13： | 1 |
| 9 | The role of the kidneys in glucose homeostasis: a new path towards normalizing glycaemia显示文摘 | DeFronzo RA Davidson JA Del Prato S | 2012 | Diabetes Obes Metab2012，14，： | 1 |
| 10 | The Spanish val- idation of level of expressed emotion scale for relatives of people with eating disorders 显示文摘 | Sepalveda AR Anastasiadou D del Maria RA | 2012 | Span J Psychol2012，15，2： | 1 |
| 11 | A new approach for separating low-molecular-weight RNA molecules by staircase electrophoresis in non-sequencing gels显示文摘 | ENCARNA VEL(A)ZQUEZ RA U1 RIVAS MAR(I)A DEL VILLAR | 2006 | Electrophoresis2006，27，： | 1 |
| 12 | Estrogen attenuatesglutamate-induced cell death by inhibiting Ca2 + infuxthrough L-type voltage-gated Ca2+ channels显示文摘 | Sribnick EA Del RA Ray SK | 2009 | Brain Res2009，1276，： | 1 |
| 13 | Sinus floor aug- mentation with simultaneous implant placement using choukroun's platelet-rich fibrin as the sole grafting ma- teriah A radiologic and histologic study at 6 months显示文摘 | Mazor Z Horowitz RA Del CM | 2009 | J Periodontol2009，80，12： | 1 |
| 14 | Immune-neuro-endocrine circuits : integrative role of cyt0kines显示文摘 | Basedovsky HO Del RA | 1992 | Frontiers in Neuroendocrinology1992，13，1： | 1 |
| 15 | Where does parkinson disease pathology begin in the brain?显示文摘 | Del Tredici K Rub U de Vos RA | 2002 | J Neuropathol Exp Neu- rol2002，61，5： | 1 |
| 16 | Coexisting preexcitation syndrome and intermittent heart block presenting as neonatal seizures 显示文摘 | KANTER RJ DEL RA MIRETTl C | 1999 | J Cardiovasc Electrophysiol1999，10，5： | 1 |
| 17 | Increased seizure threshold and severity in young transgenic CRND8 mice 显示文摘 | Del Vecchio RA Gold LH Novick S J | 2004 | Neurosci Lett2004，367，： | 1 |
| 18 | Quantitative determination of formic acid in apple juices by 1H NMR spectrometry显示文摘 | Inaki Berregi Gloria del Campo Ra'ul Caracena | 2007 | Talanta2007，72，： | 1 |
| 19 | Role of retinoic acid in lens regeneration显示文摘 | Tsonis PA Trombley MT Rowland T Chandraratna RA Del Rio-Tosnis K | 2000 | Dev Dyn2000，219，4： | 1 |
| 20 | Changes in the treatment of Enterococcus faecalis infective endocarditis in Spain in the last 15 years: from ampicillin plus gentamicin to ampicillin plus ceftriaxone 显示文摘 | Pericas JM Cervera C Del RA | 2014 | Clin Microbiol Infect2014，20，12： | 1 |
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