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| 1 | Molecular characterization and expression analysis of caveolin-1 in pig tissues显示文摘Members of the caveolin family played important roles during fundamental cellular processes,such as regulation of cell morphology,migration,and gene expression in muscle cells.In this study,caveolin-1 (Cav-1),one of the caveolins,was identified from longissimus dorsi muscle of Large Yorkshire pig and Chinese indigenous Lantang pig based on the results of mRNA differential display analysis.The deduced amino acids sequence of the porcine Cav-1 contained a caveolin domain,and was very conservative among different species.The Cav-1 mRNA was widely expressed in the eight tissues in this study,including heart,liver,kidney,encephalon,spleen,lung,longissimus dorsi muscle,and back fat, and the highest expression quantity was found in back fat of the two pig breeds.The expression quantity of porcine Cav-1 in back fat and longissimus dorsi muscle of Lantang pig was significantly higher than that of Large Yorkshire(P<0.01,and P<0.05,respectively).These results suggested that the Cav-1 might be a candidate gene for carcass traits,and might provide valuable information for understanding the mechanism of caveolae signaling in fat deposition by using the animal model of pig. | WANG Chong1,MEI YingJie 1,LI Li 1,MO DeLin 2,LI JiaQi 1,ZHANG Hao 1,TIAN XingGuo 1 &CHEN YaoSheng2 1College of Animal Science,South China Agricultural University,Guangzhou 510642,China 2School of Life Science,Sun Yat-Sen University,Guangzhou 510006,China | 2008 | Science China(Life Sciences)2008，51，7： | 2 |
| 2 | MicroRNAome Com- parison between Intramuscular and Subcutaneous Vascular Stem Cell Adipogenesis显示文摘 | Guo Yunxue Mo Delin Zhang Yue | 2012 | PLO S One2012，7，9： | 1 |
| 3 | Comparison of the longissimus muscle proteome between obese and lean pigs at 180 days显示文摘 | Anning Li Delin Mo Xiao Zhao Wei Jiang Peiqing Cong Zuyong He Shuqi Xiao Xiaohong Liu Yaosheng Chen | 2013 | Mammalian Genome2013，，1： | 1 |
| 4 | Comparison of the longissimus muscle proteome between obese and lean pigs at 180 days 显示文摘 | Li Anning Mo Delin Zhao Xiao | 2013 | MammalianGcnomc2013，24，12： | 1 |
| 5 | Notch signaling leads to a slower progression of embryonic myogenic differentiation in Landrace than in Langtang pigs显示文摘Delving into porcine embryonic myogenesis is the key to elucidate the complex regulation of breed-specific differences in growth performance and meat production.Increasing evidence proves that pigs with less meat production show earlier embryonic myogenesis,but little is known about the underlying mechanisms.In this study,we examine the longissimus dorsi muscle(LDM)by immunohistochemistry and confirm that the differentiation of myogenic progenitors is increased(P<0.05)in Lantang(LT,fatty)pigs compared with that in Landrace(LR,lean)pigs,which results in more(P<0.001)differentiated myoblasts(Pax7-/MyoD^(+))and less(P<0.001)myogenic progenitors(Pax7^(+)/MyoD^(-))in LT pigs at 35 days post-conception(35dpc).Additionally,embryonic myogenic progenitors isolated from LT pigs show greater(P<0.001)differentiation capacity with earlier expression of MyoD compared with those from LR pigs.Moreover,Notch signaling is more active(P<0.05)in LR pig myogenic progenitors than in LT pig myogenic progenitors.Inhibition of Notch signaling in LR myogenic progenitors suppresses Pax7 expression and increases MyoD expression,thus promoting myogenic differentiation.Consistently,the process of myogenic progenitors differentiating into myoblasts in ex vivo embryo limbs is accelerated when Notch signaling is inhibited.These results indicate that Notch signaling facilitates the maintenance of myogenic progenitors and antagonizes myogenic differentiation by promoting Pax7 expression and preventing MyoD expression in LR pigs. | Shufang Cai Bin Hu Qi Zhu Tianqi Duo Xiaoyu Wang Xian Tong Xiaorong Luo Renqiang Yuan Yaosheng Chen Jing Wang Chenglong Luo Baosong Xing Delin Mo | 2022 | Acta Biochimica et Biophysica Sinica2022，54，8： | 0 |
| 6 | Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs显示文摘Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor that promotes skeletal muscle growth by regulating cell proliferation and differentiation.A single nucleotide polymorphism(SNP) within intron 3 of porcine IGF2 disrupts a binding site for the repressor,zinc finger BED-type containing 6(ZBED6),leading to up-regulation of IGF2 and causing major effects on muscle growth,heart size,and backfat thickness.This favorable mutation is common in Western commercial pig populations,but absent in most Chinese indigenous pig breeds.To improve meat production of Chinese indigenous pigs,we used cytosine base editor 3(CBE3)to introduce IGF2 intron3-C3071T mutation into porcine embryonic fibroblasts(PEFs) isolated from a male Liang Guang Small Spotted pig(LGSS),and single-cell clones harboring the desired mutation were selected for somatic cell nuclear transfer(SCNT) to generate the founder line of IGF2^(T/T) pigs.Results We found the heterozygous progeny IGF2^(C/T) pigs exhibited enhanced expression of IGF2,increased lean meat by 18%-36%,enlarged loin muscle area by 3%-17%,improved intramuscular fat(IMF) content by 18%-39%,marbling score by 0.75-1,meat color score by 0.53-1.25,and reduced backfat thickness by 5%-16%.The enhanced accumulation of intramuscular fat in IGF2^(C/T) pigs was identified to be regulated by the PI3K-AKT/AMPK pathway,which activated SREBP1 to promote adipogenesis.Conclusions We demonstrated the introduction of IGF2-intron3-C3071T in Chinese LGSS can improve both meat production and quality,and first identified the regulation of IMF deposition by IGF2 through SREBP1 via the PI3KAKT/AMPK signaling pathways.Our study provides a further understanding of the biological functions of IGF2and an example for improving porcine economic traits through precise base editing. | Tianqi Duo Xiaohong Liu Delin Mo Yu Bian Shufang Cai Min Wang Ruiqiang Li Qi Zhu Xian Tong Ziyun Liang Weilun Jiang Shiyi Chen Yaosheng Chen Zuyong He | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
| 7 | E3 ligase Deltex2 accelerates myoblast proliferation and inhibits myoblast differentiation by targeting Pax7 and MyoD,respectively显示文摘E3 ubiquitin ligases are closely related to cell division,differentiation,and survival in all eukaryotes and play crucial regulatory roles in multiple biological processes and diseases.While Deltex2,as a member of the DELTEX family ubiquitin ligases,is characterized by a RING domain followed by a C-terminal domain(DTC),its functions and underlying mechanisms in myogenesis have not been fully elucidated.Here,we report that Deltex2,which is highly expressed in muscles,positively regulates myoblast proliferation via mediating the expression of Pax7.Meanwhile,we find that Deltex2 is translocated from the nucleus into the cytoplasm during myogenic differentiation,and further disclose that Deltex2 inhibits myoblast differentiation and interacts with MyoD,resulting in the ubiquitination and degradation of MyoD.Altogether,our findings reveal the physiological function of Deltex2 in orchestrating myogenesis and delineate the novel role of Deltex2 as a negative regulator of MyoD protein stability. | Qi Zhu Ziyun Liang Shufang Cai Xian Tong Xiaoyu Wang Enru Li Yaosheng Chen Delin Mo | 2023 | Acta Biochimica et Biophysica Sinica2023，55，2： | 0 |
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