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您的检索式：作者名="Dengxu Tan"
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| 1 | Numerical manifold method based on isogeometric analysis显示文摘In this study, numerical manifold method(NMM) coupled with non-uniform rational B-splines(NURBS) and T-splines in the context of isogeometric analysis is proposed to allow for the treatments of complex geometries and local refinement. Computational formula for a 9-node NMM based on quadratic B-splines is derived. In order to exactly represent some common free-form shapes such as circles, arcs, and ellipsoids, quadratic non-uniform rational B-splines(NURBS) are introduced into NMM. The coordinate transformation based on the basis function of NURBS is established to enable exact integration for the manifold elements containing those shapes. For the case of crack propagation problems where singular fields around crack tips exist, local refinement technique by the application of T-spline discretizations is incorporated into NMM, which facilitates a truly local refinement without extending the entire row of control points. A local refinement strategy for the 4-node mathematical cover mesh based on T-splines and Lagrange interpolation polynomial is proposed. Results from numerical examples show that the 9-node NMM based on NURBS has higher accuracies. The coordinate transformation based on the NURBS basis function improves the accuracy of NMM by exact integration. The local mesh refinement using T-splines reduces the number of degrees of freedom while maintaining calculation accuracy at the same time. | ZHANG YouLiang LIU DengXue TAN Fei | 2015 | Science China(Technological Sciences)2015，58，9： | 4 |
| 2 | Establishment of organoid models for pancreatic ductal adenocarcinoma and screening of individualized therapy strategy显示文摘Background:Patients with pancreatic ductal adenocarcinoma(PDAC)who undergo surgical resection and receive effective chemotherapy have the best chance for longterm survival.Unfortunately,because of the heterogeneity of pancreatic cancer,it is difficult to find a personalized treatment strategy for patients.Organoids are ideal preclinical models for personalized medicine.Therefore,we explore the cultivation conditions and construction methods of PDAC organoid models to screen the individualized therapy strategy.Methods:Fresh PDAC tissues from surgical resection were collected and digested with digestive enzymes;then the tumor cells were embedded in Matrigel with a suitable medium to establish the PDAC organoid models.The genetic stability of the organoids was analyzed using whole exon sequencing;hematoxylin and eosin staining and immunohistochemistry of organoids were performed to analyze their consistency with the pathological morphology of the patient's tumor tissue;After 2 days of organoid culture,we selected four commonly used clinical chemotherapy drugs for single or combined treatment to analyze drug sensitivity.Results:Two cases of PDAC organoid models were successfully established,and the results of their pathological characteristics and exome sequencing were consistent with those of the patient's tumor tissue.Both PDAC organoids showed more sensitivity to gemcitabine and cisplatin,and the combined treatment was more effective than monotherapy.Conclusion:Both organoids better retained the pathological characteristics,genomic stability,and heterogeneity with the original tumor.Individual PDAC organoids exhibited different sensitivities to the same drugs.Thus,this study provided ideal experimental models for screening individualized therapy strategy for patients with PDAC. | Miaomiao Gong Han Meng Dengxu Tan Peng Li Jing Qin Qingling An Changhong Shi Jiaze An | 2023 | Animal Models and Experimental Medicine2023，6，5： | 0 |
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