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| 1 | High-resolution stalagmite δ13C record of soil processes from southwestern China during the early MIS 3显示文摘Knowledge of the evolution of pedogenic processes in the carbonate area is important to understand the dynamics of rock desertification.A precisely-dated stalagmite δ13C record between 61.3 and 50.5 ka BP from southwestern China,potentially related to soil biogenic CO2 production,shifts within a narrow range between -9‰ and -7‰,and exhibits a prominent cycle of 350-470 a,independent of the Asian monsoon changes.This indicates that a threshold effect might control the amplitude of calcite δ13C variability,likely associated with the vertical soil loss in the carbonate area.The periodic δ13C changes reflect that the loss/recovery of soil cover can operate on centennial scale.Thus,the rock desertification in southwestern China might at least initiate 60 ka ago and should be of natural origin. | LIU DianBing WANG YongJin CHENG Hai EDWARDS R L | 2013 | Chinese Science Bulletin2013，58，7： | 5 |
| 2 | Design and biosynthesis of functional protein nanostructures显示文摘Proteins are one of the major classes of biomolecules that execute biological functions for maintenance of life.Various kinds of nanostructures self-assembled from proteins have been created in nature over millions of years of evolution,including protein nanowires,layers and nanocages.These protein nanostructures can be reconstructed and equipped with desired new functions.Learning from and manipulating the self-assembly of protein nanostructures not only help to deepen our understanding of the nature of life but also offer new routes to fabricate novel nanomaterials for diverse applications.This review summarizes the recent research progress in this field,focusing on the characteristics,functionalization strategies,and applications of protein nanostructures. | Feng Li Dianbing Wang Juan Zhou Dong Men Xian-En Zhang | 2020 | Science China(Life Sciences)2020，63，8： | 4 |
| 3 | Asian Hydroclimate Changes and Mechanisms in the Preboreal from an Annually-laminated Stalagmite, Daoguan Cave, Southern China显示文摘One-year-resolved and annually-counted stalagmite multi-proxies(δ^(18)O, δ^(13)C, and layer width) from Daoguan Cave, Guizhou Province revealed detailed variability regarding the Asian Summer Monsoon(ASM) and local humidity across Bond events(BE) in the Preboreal. During BEs 8 and 7, 1.5‰ enrichments in δ^(18)O values were generally consistent with high-to low-latitude climate changes. In detail, the decadal-scale minor δ^(18)O oscillations in BE8 were broadly less than the mean value, in contrast to the significant changes in local soil moisture derived from the δ^(13)C values and layer records. In the mid-BE7, δ^(18)O variability was generally above the average level, and higher-amplitude variations were observed in the three proxy indicators. Wavelet analysis on the total δ^(18)O time series and across the specific time windows of BEs 8 and 7 identified periodicities of about 130, 60, and 20-a, respectively. Exceptionally strong in BE7, the 60-a cycle, pervasively observed in instrumental studies, became prominent starting at 11.4 kaBP. Thus, glacial background conditions are important for suppressing the ASM intensity in BE8, while during BE7, tropical hydrological circulations were potentially actively involved. Consequently, climate internal oscillations, analogous to modern conditions, might have occurred in the distant past once the link between the tropical ocean and atmosphere was established as occurs today. | LIU Shushuang LIU Dianbing WANG Yongjin ZHAO Kan | 2018 | Acta Geologica Sinica(English Edition)2018，92，1： | 3 |
| 4 | A MATHEMATICAL MODEL FOR THE PROCESS OF MAGNETITE OXIDATION显示文摘The oxidation rate of magnetite concentrate is ery quick at the reaction beginning,but itbecomes muck slower when the reaction is conducted to a certain level because the productlayer on the micro particles resists the oxygen diffusion greatly This phenomena are provedthrough experiments.And a function is put forward to calculating the quivalent osmosingthickness of the product layer.Based on the function,a mathematical model is establishedfor the whole range reaction process of magnetite in pellets.It is proved that this model fitsthe real process very well under any condition. | HUANG Dianbing, KONG Lingtan, LIN ZongcaiUniversity of Science and Technology Beijing, Beijing. China | 1994 | Acta Metallurgica Sinica(English Letters)1994，7，1： | 2 |
| 5 | A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells显示文摘Apoptosis is a form of programmed cell death that is essential for maintaining internal environmental stability.Disordered apoptosis can cause a variety of diseases;therefore,sensing apoptosis can provide help in study of mechanism of the relevant diseases and drug development.It is known that caspase-3 is a key enzyme involved in apoptosis and the expression of its activity is an indication of apoptosis.Here,we present a genetically encoded switch-on m Neon Green2-based molecular biosensor.m Neon Green2 is the brightest monomeric green fluorescent protein.The substrate of caspase-3,DEVD amino acid residues,is inserted in it,while cyclized by insertion of Nostoc punctiforme Dna E intein to abolish the fluorescence(inactive state).Caspase-3-catalyzed cleavage of DEVD linearizes m Neon Green2 and rebuilds the natural barrel structure to restore the fluorescence(activated state).The characterization exhibited that the Caspase-3 biosensor has shortened response time,higher sensitivity,and prolonged functional shelf life in detection of caspase-3 amongst the existing counterparts.We also used the Caspase-3 biosensor to evaluate the effect of several drugs on the induction of apoptosis of He La and MCF-7 tumor cells and inhibition of Zika virus invasion. | Rui Gong Dianbing Wang Ghulam Abbas Shimin Li Qian Liu Mengmeng Cui Xian-En Zhang | 2022 | Science China(Life Sciences)2022，65，3： | 2 |
| 6 | Rapid detection of Bacillus anthracis using monoclonal antibody fmlctionalized QCM sensor显示文摘 | Rongzhang Hao Dianbing Wang Xian' en Zhang | 2009 | Biosensors and Bioelectronics2009，24，： | 1 |
| 7 | Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells显示文摘 | Hua Li Sha Wei Yuan Fang Min Li Xia Li Zhe Li Jibin Zhang Guofeng Zhu Chuanyou Li Lijun Bi Guimin Zhang Dianbing Wang Xian-En Zhang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，9： | 1 |
| 8 | A detailed comparison of Asian Monsoon intensity and Greenland temperature during the Aller?d and Younger Dryas events显示文摘 | Dianbing Liu Yongjin Wang Hai Cheng R. Lawrence Edwards Xinggong Kong Xianfeng Wang Jiangying Wu Shitao Chen | 2008 | Earth and Planetary Science Letters2008，，3： | 1 |
| 9 | Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months显示文摘Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection,the protection period of vaccination and the possibility of building an active herd immunity.This retrospective cohort study included 172 COVID-19 patients who were hospitalized in Wuhan.A total of404 serum samples were obtained over six months from hospitalization to convalescence.Antibodies in the specimens were quantitatively analyzed by the capture chemiluminescence immunoassays(CLIA).All patients were positive for the anti-SARS-Co V-2 Ig M/Ig G at the onset of COVID-19 symptoms,and the Ig G antibody persisted in all the patients during the convalescence.However,only approximately 25%of patients can detect the Ig M antibodies,Ig M against N protein(NIg M)and receptor binding domain of S protein(RBD-Ig M)at the 27 th week.The titers of Ig M,N-Ig M and RBD-Ig M reduced to 16.7%,17.6%and 15.2%of their peak values respectively.In contrast,the titers of Ig G,N-Ig G and RBD-Ig G peaked at 4–5 th week and reduced to 85.9%,62.6%and 87.2%of their peak values respectively at the end of observation.Dynamic behavior of antibodies and their correlation in age,gender and severity groups were investigated.In general,the COVID-19 antibody was sustained at high levels for over six months in most of the convalescent patients.Only a few patients with antibody reducing to an undetectable level which needs further attention.The humoral immune response against SARS-Co V-2 infection in COVID-19 patients exhibits a typical dynamic of acquired immunity. | Yang Zheng Qing Zhang Ashaq Ali Ke Li Nan Shao Xiaoli Zhou Zhiqin Ye Xiaomin Chen Shanshan Cao Jing Cui Juan Zhou Dianbing Wang Baidong Hou Min Li Mengmeng Cui Lihua Deng Xinyi Sun Qian Zhang Qinfang Yang Yong li Hui Wang Yake Lei Bo Yu Yegang Cheng Xiaolin Tong Dong Men Xian-En Zhang | 2021 | Virologica Sinica2021，36，5： | 1 |
| 10 | A detailed comparison of Asian Monsoon intensity and Greenland temperature during the Allerφd and Younger Dryas events显示文摘 | Liu Dianbing Wang Yongjin Cheng Hai Edwards R L Kong Xinggong Wang Xianfeng Wu Jiangying Chen Shitao | | 0，，： | 1 |
| 11 | HIV-1 viral cores enter the nucleus collectively through the nuclear endocytosis-like pathway显示文摘It is recognized that HIV-1 capsid cores are disassembled in the cytoplasm, releasing their genomes into the nucleus through nuclear pores, but there is also evidence showing the capsid(CA) exists in the nucleus. Whether HIV-1 enters the nucleus and how it enters the nucleus through the undersized nuclear pore remains mysterious. Based on multicolor labeling and real-time imaging of the viral and cellular components, our observations via light and electron microscopy suggest that HIV-1 selectively gathered at the microtubule organization center(MTOC), leading the nearby nuclear envelope(NE) to undergo deformation,invagination and restoration to form a nuclear vesicle in which the viral particles were wrapped;then, the inner membrane of the nuclear vesicle ruptured to release HIV-1 into the nucleus. This unexpected discovery expands our understanding of the complexity of HIV-1 nuclear entry, which may provide new insights to HIV-1 virology. | Xia Li Dianbing Wang Zongqiang Cui Qin Li Min Li Yingxin Ma Qinxue Hu Yikai Zhou Xian-En Zhang | 2021 | Science China(Life Sciences)2021，64，1： | 1 |
| 12 | Catalytic activity, stability and structure of multi-walled carbon nanotubes in the wet air oxidation of phenol 显示文摘 | Yang Shaoxia Li Xiang Zhu Wanpeng Wang dianbing Descorme Claude | 2008 | Carbon2008，46，3： | 1 |
| 13 | Raman spectra of the GFP-like fluorescent proteins显示文摘The objective of the study was to elucidate optical characteristics of the chromophore structures of fluorescent proteins.Raman spectra of commonly used GFP-like fluorescent proteins (FPs)with diverse emission wavelengths (green,yellow,cyan and red),including the enhanced homogenous FPs EGFP, EYFP,and ECFP (from jellyfish)as well as mNeptune (from sea anemone)were measured.High-quality Raman spectra were obtained and many marker bands for the chromophore of the FPs were identified via assignment of Raman spectra bands.We report the presence of a positive linear correlation between the Raman band shift of Cs=C6 and the excitation energy of FPs,demonstrated by plotting absorption maxima (cm-1)against the position of the Raman band Cs=C6 in EGFP,ECFP,EYFP,the anionic chromophore and the neutral chromophore.This study revealed new Raman features in the chromophores of the observed FPs,and may contribute to a deeper understanding of the optical properties of FPs. | Ye Yuan Dianbing Wang Jibin Zhang Ji Liu Jian Chen Xian-En Zhang | 2018 | Biophysics Reports2018，4，5： | 0 |
| 14 | Illuminating single genomic loci in live cells by reducing nuclear background fluorescence显示文摘The tagging of genomic loci in living cells provides visual evidence for the study of genomic spatial organization and gene interaction.CRISPR/dCas9(clustered regularly interspaced short palindromic repeats/deactivated Cas9)labeling system labels genes through binding of the dCas9/sgRNA/fluorescent protein complex to repeat sequences in the target genomic loci.However,the existence of numerous fluorescent proteins in the nucleus usually causes a high background fluorescent readout.This study aims to limit the number of fluorescent modules entering the nucleus by redesigning the current CRISPR/dCas9-SunTag labeling system consisting of dCas9-SunTag-NLS(target module)and scFv-sfGFP-NLS(signal module).We removed the nuclear location sequence(NLS)of the signal module and inserted two copies of EGFP into the signal module.The ratio of the fluorescent intensity of the nucleus to that of the cytoplasm(N/C ratio)was decreased by 71%,and the ratio of the signal to the background(S/B ratio)was increased by 1.6 times.The system can stably label randomly selected genomic loci with as few as 9 repeat sequences. | Song Lu Dianbing Wang Yu Hou Dongge Guo Yulin Deng Xian-En Zhang | 2021 | Science China(Life Sciences)2021，64，5： | 0 |
| 15 | Genetically encoded redox biosensor system for H_(2)O_(2) measurement in four subcellular compartments in acute myeloid leukemia(AML) cells显示文摘Dear Editor,Acute myeloid leukemia (AML) is an aggressive form of a hematological neoplastic disorder induced by the oncogenic transformation of hematopoietic stem and myeloid progenitor cells (Bossis et al., 2014). | Mengmeng Cui Ghulam Abbas Dianbing Wang Qian Liu Rui Gong Min Li Xian-En Zhang | 2022 | Science China(Life Sciences)2022，65，6： | 0 |
| 16 | High-resolution stalagmite δ^(13)C record of soil processes from southwestern China during the early MIS 3显示文摘Knowledge of the evolution of pedogenic processes in the carbonate area is important to understand the dynamics of rock desertification.A precisely-dated stalagmite δ13C record between 61.3 and 50.5 ka BP from southwestern China,potentially related to soil biogenic CO2 production,shifts within a narrow range between -9‰ and -7‰,and exhibits a prominent cycle of 350-470 a,independent of the Asian monsoon changes.This indicates that a threshold effect might control the amplitude of calcite δ13C variability,likely associated with the vertical soil loss in the carbonate area.The periodic δ13C changes reflect that the loss/recovery of soil cover can operate on centennial scale.Thus,the rock desertification in southwestern China might at least initiate 60 ka ago and should be of natural origin. | LIU DianBing WANG YongJin CHENG Hai EDWARDS R L | 2013 | Science China(Technological Sciences)2013，56，3： | 0 |
| 17 | Altered hACE2 binding affinity and S1/S2 cleavage efficiency of SARS-CoV-2 spike protein mutants affect viral cell entry显示文摘SARS-CoV-2 variants are constantly emerging,hampering public health measures in controlling the number of infections.While it is well established that mutations in spike proteins observed for the different variants directly affect virus entry into host cells,there remains a need for further expansion of systematic and multifaceted comparisons.Here,we comprehensively studied the effect of spike protein mutations on spike expression and proteolytic activation,binding affinity,viral entry efficiency and host cell tropism of eight variants of concern(VOC)and variants of interest(VOI).We found that both the full-length spike and its receptor-binding domain(RBD)of Omicron bind to hACE2 with an affinity similar to that of the wild-type.In addition,Alpha,Beta,Delta and Lambda pseudoviruses gained significantly enhanced cell entry ability compared to the wild-type,while the Omicron pseudoviruses showed a slightly increased cell entry,suggesting the vastly increased rate of transmission observed for Omicron variant is not associated with its affinity to hACE2.We also found that the spikes of Omicron and Mu showed lower S1/S2 cleavage efficiency and inefficiently utilized TMPRSS2 to enter host cells than others,suggesting that they prefer the endocytosis pathway to enter host cells.Furthermore,all variants'pseudoviruses we tested gained the ability to enter the animal ACE2-expressing cells.Especially the infection potential of rats and mice showed significantly increased,strongly suggesting that rodents possibly become a reservoir for viral evolution.The insights gained from this study provide valuable guidance for a targeted approach to epidemic control,and contribute to a better understanding of SARS-CoV-2 evolution. | Ke Wang Yu Pan Dianbing Wang Ye Yuan Min Li Yuanyuan Chen Lijun Bi Xian-En Zhang | 2023 | Virologica Sinica2023，38，4： | 0 |
| 18 | Kinetics of Reduction of Iron Oxide in CaO-SiO_2-Al_2O_3-Fe_tO Slags with Graphite Powder显示文摘The reduction rate of Fe_tO in CaO-SiO_2-Al_2O_3-Fe_tO slags with graphite powder has been determined in an alumina crucible at 1 673-1 823 K.The effects of temperature,slag basicity and Fe_tO content on the reduction rate have also been discussed.Test results show that the reduction rate increases with the increase of temperature or Fe_tO concentration in slags,and the reduction rate has a parabolic relation with slag simple basicity or optical basicity.The maximum reduction rate is observed around CaO/SiO_2 = l.5 of molten slags.The reaction order is 1.26 or 1.31,and the reduction activation energy is 126.8 or 129.7 kJ/mol respectively in regard to Fe_tO content or Fe_tO activity calculated by using regular solution model.The reduction rate of Fe_tO in CaO-SiO_2-Al_2O_3-Fe_tO slags with graphite powder is in the range of 0.15×10^(-4)- 0.86×10^(-4) mol-O/cm^2 · s. | Yang Xuemin Huang Dianbing Kong Lingtan Yang Tianjun Xie Yusheng Wang Daguang | 1998 | Journal of Iron and Steel Research(International)1998，5，1： | 0 |
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