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| 1 | Chromosome-scale scaffolding of the black raspberry (Rubus occidentalis L.) genome based on chromatin interaction data显示文摘Black raspberry(Rubus occidentalis L.)is a niche fruit crop valued for its flavor and potential health benefits.The improvement of fruit and cane characteristics via molecular breeding technologies has been hindered by the lack of a high-quality reference genome.The recently released draft genome for black raspberry(ORUS 4115-3)lacks assembly of scaffolds to chromosome scale.We used high-throughput chromatin conformation capture(Hi-C)and Proximity-Guided Assembly(PGA)to cluster and order 9650 out of 11,936 contigs of this draft genome assembly into seven pseudo-chromosomes.The seven pseudo-chromosomes cover~97.2%of the total contig length(~223.8 Mb).Locating existing genetic markers on the physical map resolved multiple discrepancies in marker order on the genetic map.Centromeric regions were inferred from recombination frequencies of genetic markers,alignment of 303 bp centromeric sequence with the PGA,and heat map showing the physical contact matrix over the entire genome.We demonstrate a high degree of synteny between each of the seven chromosomes of black raspberry and a high-quality reference genome for strawberry(Fragaria vesca L.)assembled using only PacBio long-read sequences.We conclude that PGA is a cost-effective and rapid method of generating chromosome-scale assemblies from Illumina short-read sequencing data. | Rubina Jibran Helge Dzierzon Nahla Bassil Jill M.Bushakra Patrick P.Edger Shawn Sullivan Chad E.Finn Michael Dossett Kelly J.Vining Robert VanBuren Todd C.Mockler Ivan Liachko Kevin M.Davies Toshi M.Foster David Chagné | 2018 | Horticulture Research2018，5，1： | 4 |
| 2 | Genetic and genomic resources for Rubus breeding:a roadmap for the future显示文摘Rubus fruits are high-value crops that are sought after by consumers for their flavor,visual appeal,and health benefits.To meet this demand,production of red and black raspberries(R.idaeus L.and R.occidentalis L.),blackberries(R.subgenus Rubus),and hybrids,such as Boysenberry and marionberry,is growing worldwide.Rubus breeding programmes are continually striving to improve flavor,texture,machine harvestability,and yield,provide pest and disease resistance,improve storage and processing properties,and optimize fruits and plants for different production and harvest systems.Breeders face numerous challenges,such as polyploidy,the lack of genetic diversity in many of the elite cultivars,and until recently,the relative shortage of genetic and genomic resources available for Rubus.This review will highlight the development of continually improving genetic maps,the identification of Quantitative Trait Loci(QTL)s controlling key traits,draft genomes for red and black raspberry,and efforts to improve gene models.The development of genetic maps and markers,the molecular characterization of wild species and germplasm,and highthroughput genotyping platforms will expedite breeding of improved cultivars.Fully sequenced genomes and accurate gene models facilitate identification of genes underlying traits of interest and enable gene editing technologies such as CRISPR/Cas9. | Toshi M.Foster Nahla V.Bassil Michael Dossett Margaret Leigh Worthington Julie Graham | 2019 | Horticulture Research2019，6，1： | 2 |
| 3 | Obesity and pulmonary complications in critically injured adults显示文摘 | Dossett LA Heffernan D Liqhtfoot M | | 0，，5： | 1 |
| 4 | Work-measurement labor standards: The state of the art显示文摘 | Royal J Dossett | 1995 | Industrial Engineering1995，27，4： | 1 |
| 5 | Imple- mentation of a real--time compliance dashboard to help reduce SICU ventilator-associated pneumonia with the ventilator bundle显示文摘 | Zaydfudim V Dossett L A Starmer J M | 2009 | Arch Surg2009，144，7： | 1 |
| 6 | Work-measurement labor standards :The state of the art显示文摘 | Royal J Dossett | 1995 | Industrial Engineering1995，27，4： | 1 |
| 7 | Rubus fruit phenolic research:the good,the bad,and the confusing显示文摘 | Lee J Dossett M Finn CE | 2012 | Food Chemistry2012，130，： | 1 |
| 8 | Early prediction of massive transfusion in trauma:simple as ABC (assessment of blood consumption)?显示文摘 | NunezTC Voskresensky Ⅳ Dossett LA | 2009 | J Trauma2009，66，2： | 1 |
| 9 | Extraction ofgenomic DNAsuitable for PCR analysis from died plant rclnzomes roots显示文摘 | TAN S L DOSSETT M KATZE M G | 1998 | BioTech1998，25，5： | 1 |
| 10 | Extraction of genomic DNA suitable for PCR analysis from dried plant rhizomes/roots显示文摘 | Tan S L Dossett M Katze M G | 1998 | Biotech1998，25，5： | 1 |
| 11 | Revisiting the validity of APACHE lI in the trauma ICU : improved risk stratification in critically injured adults 显示文摘 | Dossett LA Rcdhage LA Sawyer RG | 2009 | Injury2009，40，9： | 1 |
| 12 | Risk factors fortreatment failure in patients receiving vancomycin for hospital-acquired methicillin-resistant Staphylococcus aureus pneumo-nia显示文摘 | Aston JL Dortch MJ Dossett LA | 2010 | Surg Infect(Larchmt)2010，11，1： | 1 |
| 13 | Adoptive immunotherapy:engineering T cell responses as biologic weapons for tumor mass destruction显示文摘 | Ho WY Blattman JN Dossett ML | | 0，，05： | 1 |
| 14 | Obesity and pulmonarycomplications in critically injured adults显示文摘 | Dossett L A Heffernan D Liqhtfoot M | 2008 | Chest2008，134，5： | 1 |
| 15 | Extraction of genomic DNA suitable for PCR analysis from dried plant rhizomes/roots显示文摘 | Tan S L Dossett M Katze M G | | 0，，05： | 1 |
| 16 | Revisiting the validity of APACHE II in the trauma tCU: improved risk stratification in crit- ically injured adults显示文摘 | Dossett LA Redhage LA Sawyer RG | 2009 | Injury2009，40，9： | 1 |
| 17 | Estradiol is associated with mortality in critically ill trauma and surgical patients显示文摘 | MAY A K DOSSETT L A NORRIS P R | 2008 | Crit Care Med2008，36，1： | 1 |
| 18 | Blood glucose variability is associated with mortality in the surgical intensive care unit显示文摘 | Dossett L Cao H Dortch M | 2008 | Am Surg2008，74，8： | 1 |
| 19 | Serum estradiol concentration as a predictor of death in critically ill and injured adults显示文摘 | DOSSETT L A SWENSON B R EVANS H L | 2008 | Surgical Infections2008，9，1： | 1 |
| 20 | Early prediction of massive transfusion in trauma: simple as ABC (assessment of blood consumption) 显示文摘 | Nunez TC Voskresensky IV Dossett LA | 2009 | J Trauma2009，66，2： | 1 |
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