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| 1 | Mapping of Fertility Restoring Gene for Aegilops kotschyi Cytoplasmic Male Sterility in Wheat Using SSR Markers显示文摘LK783 was found to be a good fertility restorer for K-type male sterility of wheat. Microsatellite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among (KJ5418A//911289/LK783)F1 population,respectively. Seventy-nine sets of SSR primers were screened for polymorphism between the two pools, 6 of which were found polymorphic. Linkage analysis showed that Xgwm11, Xgwm18 , Xgwm264a and Xgwm273were linked to the restoring gene in LK783, while Xgwm11, Xgwm18 and Xgwm273 were co-segregated. The distance between the Rf gene in LK783 and the three co-segregated markers was 6.54±4.37 cM, the distance between Rf gene and Xgwm264a was 5.71±4.10 cM. The four SSR markers were located on chromosome 1BS by amplifying the DNA of nulli-tetrasomics and ditelosomics of CS with the 4 sets of primers, indicating that the major restoring gene in LK783 was located on 1BS, but the relative location of the gene was different from Rfv1, allelism of the two genes should be further investigated. The breeding for new fertility restorer lines of K-type cytoplasmic male sterility in wheat would be facilitated by using the four polymorphic markers. | LIU Bao-shen, SUN Qi-xin, GAO Qing-rong, SUN Lan-zhen, XIE Chao-jie, LI Chuan-you, NI Zhong-fu and DOU Bing-de( Agronomy Department, Shandong Agricultural University, Taian 271018, P.R.China Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094 , P. R . China ) | 2002 | Agricultural Sciences in China2002，1，8： | 13 |
| 2 | Research on Isolation and Clone of Embryonic Stem Cell-Like in Bovine显示文摘Bovine embryonic stem cell would be invaluable for researching the aspect of animal cloning, production transgenic animal and discussion of gene function in vitro. With the object of establishing an effective culture system for isolation and clone of bovine pluripotent stem cell, we cultured bovine embryos and mouse embryos including morula blastula and hatached blastula and obtained animal ICM on Primary marine embryonic fibroblast (Primary murine embryonic fibroblast, PMEF) feeder layer with tissue medium(DMEM supplemented with 15ml/100ml NBS ,0.1μmol/L Na2SeO3, 0. 1mmol/L β-mercaptoethanol, 1 000ng/ml LIF,10 ng/ml IGF, 1mmol/L necessary amino acid and 1mmol/L L-glutamine), then, we obtained mouse ICM and bovine ICM. Moreover, we isolated and cloned the 6 passage bovine ES like cells(12 cell lines) and 9 passage marine ES like cells (52 cell lines) deriving from bovine ICM and murine ICM respectively on the feeder layer of PMEF by disaggregating ICM and ES cell clones of bovine and murine into smaller clumps through digesting with 0. 125g/100ml trypsin and 0.02g/100ml EDTA and scattering with a glass needle. The pluripotency of both murine and bovine ES like cells was identified with morphological character, histochemistry identification, karyotype analysis and differentiation of ES cells in vitro or in vivo. This result showed that bovine embryonic stem cell and murine embryonic stem cell had developmental pluripotency. | AN Li-long, YANG Qi, XIAO Mei, FENG Xiu-Liang, YANG Chun-rong, LEI An-min, GAO Zhi-min, DOU Zhong-ying and QIU Huai( Agriculture College of Zhanjiang Ocean University, Zhanjiang 524008 , P. R . China Key laboratory of AnimalReproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China , Northwestern Scienceand Technology University of Agriculture and Forestry, yangling 712100 , P.R. China) | 2002 | Agricultural Sciences in China2002，1，4： | 2 |
| 3 | Single nucleotide polymorphisms in genes associated with isoniazid resistance in mycobacterium tuberculosis显示文摘 | RAMASWAMY S V REICH R DOU S J | 2003 | Antimicrob Agents Chemother2003，47，： | 2 |
| 4 | Effect of a free-range rais- ing system on growth performance, carcass yield, and meat quali- ty of slow-growing chicken显示文摘 | Wang K H Shi S R Dou T C | 2009 | Poult Sci2009，88，10： | 1 |
| 5 | Determination of benzene,toluene,ethylbenzene,xylenes in water at sub-ng L-1 levels by solid-phase microextraction coupled to cryo-trap gas chromatography-mass spectrometry显示文摘 | Lee M R Chang C M Dou J P | 2007 | Chemosphere2007，69，： | 1 |
| 6 | Optimum Design of 2-DOF Parallel Manipulators with Actuation Redundancy 显示文摘 | Zhu Z S Dou R L | 2009 | Mechatronics2009，19，5： | 1 |
| 7 | Neuronatin mRNA: alternatively spliced forms of a novel brain-specific mammalian developmental gene 显示文摘 | Joseph R Dou D Tsang W | 1995 | Brain Res1995，690，： | 1 |
| 8 | Investigation of theInteraction Between Isomeric Derivatives and HumanSerum Albumin by Fluorescence Spectroscopy and Mo-lecular Modeling 显示文摘 | WANG R’DOU H YIN Y | 2014 | Journal of Luminescence2014，154，： | 1 |
| 9 | Dramatically Accelerated Synthesis of b-Aminoketones via Aqueous Mannich Reaction under Combined Microwave and Ultrasound Irradiation显示文摘 | Peng Y Dou R Song G | 2005 | Synlett2005，14，： | 1 |
| 10 | Relation ship between the methylation status of dietary fla vonoids and their growth inhibitory and apoptosis in ducing activities in human cancer cells 显示文摘 | Landis Piwowar K R Milacic V Dou Q P | 2008 | J Cell Bio chem2008，105，2： | 1 |
| 11 | Development of Al added high-Cr ODS steels for fuel cladding of next generation nuclear systems显示文摘 | Kimura A Kasada R Iwata N Kishimoto H Zhang C H Isselin J Dou P Lee J H Muthukumar N Okuda T Inoue M Ukai S Ohnuki S Fujisawa T Abe F | | 0，，1： | 1 |
| 12 | Analysis of the constituents in rat plasma after oral administration of Shexiang Baoxin pill by HPLCESI-MS/MS显示文摘 | Jiang P Liu R Dou S | 2009 | Biomed Chromatogr2009，23，12： | 1 |
| 13 | Application of the regularization method to the inverse black body radiation problem显示文摘 | DOU L HODGSON R J W | 1992 | IEEE Trans Antennas Propagat1992，40，10： | 1 |
| 14 | 黄、东海鳀鱼及其他经济鱼类资源声学评估的调查研究——“北斗”号1984年11月至1989年1月调查研究报告 第一章 基本情况显示文摘 | FISHERIES INVESTIGATIONS BY R/V “BEI DOU”Edited by Zhu De-Shah and Svein A.IversenYellow Sea rishcrics Research Institute. QingdaoInstitute r f Marine Research Bergen | 1990 | 海洋水产研究1990，，11： | 1 |
| 15 | The ginsenosides and carbohy- drate profiles of ginseng cultivated under mountainous forest 显示文摘 | Zhang J Gao R Dou D | 2013 | Pharmacogn Mag2013，9，36： | 1 |
| 16 | Pollution characteristics of Pb, Zn, As, Cd in the Bijiang River显示文摘 | Yi Q Dou X D Huang Q R | 2012 | Procedia Environmental Sciences2012，13，： | 1 |
| 17 | Transcriptome and expressionprofiling analysis of Leuciscus waleckii: an explorationof the alkali-adapted mechanisms of a freshwaterteleost显示文摘 | Chang Y M Tang R Dou X J | 2014 | Mol Biosyst2014，10，： | 1 |
| 18 | Application of the regularization methods to the inverse black body radiation problem显示文摘 | Dou Lixin Hodgson R J W | 1992 | IEEE Trans Antennas and Propagation1992，40，10： | 1 |
| 19 | Evolution of microstructure and mechanical properties of cold-rolled SUS430 stainless steel during a continuous annealing process显示文摘 | Zhang X Wen Z Dou R F | 2014 | Materials Science and Engineering A2014，598，： | 1 |
| 20 | Fault linkage patterns and their control on the formation of the petroleum systems of the Erlian Basin, Eastern China 显示文摘 | Dou L R Chang L | 2003 | Marine and Pe- troleum Geology2003，22，20： | 1 |
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