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您的检索式：作者名="Duan Chengguo"
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| 1 | Regulation of microRNA on plant development and viral infection显示文摘MicroRNAs (miRNAs ) 是非编码规章的 RNAswhich 的约 22 nt 的一个班在优核质是通用的。他们为劈开或翻译压抑由 targetingmRNAs 充当基因表示的否定管理者。miRNAs 广泛地参予与植物联系的基因表示的规定发展过程,例如在植物的机关形态发生和 signaltransduction 小径。类似于在植物的抗病毒的 RNA silencing, miRNA 小径被 silencing 也介入压制病毒编码的 ors。在这评论,我们给调停 miRNA 的规章的小径和调停 siRNA 的 RNA silencing 之间的差别的一篇简短摘要。Inparticular,我们在 miRNA 上考察最近的研究规章的角色在种发展进程,以及干扰病毒在 miRNA 小径压制 ors,试图在真核细胞的有机体把卓见装入基因表达式规定的复杂性。 | DUAN Chengguo WANG Chunhan GUO Huishan | 2006 | Chinese Science Bulletin2006，51，3： | 7 |
| 2 | Artificial microRNAs highly accessible to targets confer efficient virus resistante in plants显示文摘 | Duan Chengguo Warn Chunhan Fang Rongxiang | 2008 | J Virol2008，82，11： | 1 |
| 3 | Delayed resistance to Cucumber mosaic virus mediated by 3′ UTR-derived hairpin RNA显示文摘RNA silencing has been shown to function in the plant antivirus defense response,leading to viral RNA degradation induced by vsiRNA-containing RISC cleavage activity.Cucumber mosaic virus(CMV) 3′UTR sequences share a high conservation of nucleotide sequence and secondary structures that are important for CMV replication.Here,in an attempt to simultaneously target the multiple genomic and subgenomic RNAs of CMV for degradation,CMV 3′UTR were used to design hairpin RNA(hpRNA) to transform tobacco(Xanthi.nc) so as to constitutively produce viral siRNAs.Most of the transgenic plants expressing CMV Q strain(Q-CMV,subgroup Ⅱ strain) RNA3 3′UTR-derived hpRNA showed de-layed resistance to Q-CMV infection and exhibited recovery phenotypes.Compared with Q-CMV-in-oculated leaves,the upper leaves showed weak or no disease symptoms and a reduced accumulation level of viral RNAs.Together with transient assays,our results indicate that the 3′UTR-derived siRNAs were biologically active in targeting viral RNA for degradation.Recovery resistance in transgenic plants was also observed against subgroup IB strain SD-CMV infection,indicating a broad-spectrum anti-CMV effect of the 3′UTR-based antiviral silencing.Northern blot assays indicated that there was no strong correlation between the degree of resistance and the accumulation level of 3′UTR-derived siRNAs,suggesting that to target a highly structured RNA,such as the CMV 3′UTR,the quantity of siRNAs may not be the only determinant of silencing efficiency.Target RNA secondary structures may also affect target accessibility,siRNA-containing RISC-target recognition and the consequent antiviral effect. | DUAN ChengGuo WANG ChunHan Guo HuiShan | 2008 | Chinese Science Bulletin2008，53，21： | 0 |
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