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| 1 | Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves显示文摘A new ribosome-inactivating protein(RIP)with a molecular weight of 31 kDa induced by Cinchonaglycoside C(1)designatedCLP31,was isolated from tobacco leaves.Analysis of this protein sequence indicated that it belongs to the RIP family and itwas distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence.CIP31 can directly impairsynthesis of coat protein(CP)of tobacco mosaic virus(TMV),which resulted in inhibition of TMV long distance movementand multiplication in tobacco plants at concentrations of ng/mL.Furthermore,no toxicity was shown to the growth andfertility of the plants.CIP31 was synthesized only in the presence of Cinchonaglycoside C(1)and was independent of thesalicylic acid(SA)signal pathway.We provided evidence for the SA-independent biological induction of resistance. | Yanmei Li Yantao Jia Zhongkai Zhang Xiaoying Chen Hongping He Rongxiang Fang Xiaojiang Hao | 2007 | Journal of Integrative Plant Biology2007，49，9： | 8 |
| 2 | Interkingdom signaling in plant-microbe interactions显示文摘The widespread communications between prokaryotes and eukaryotes via signaling molecules are believed to affect gene expression in both partners.During the communication process,the contacted organisms produce and release small molecules that establish communication channels between two kingdoms—this procedure is known as interkingdom signaling.Interkingdom communications are widespread between pathogenic or beneficial bacteria and their host plants,with diversified outcomes depending on the specific chemical-triggered signaling pathways.Deciphering the signals or language of this interkingdom communication and uncovering the underlying mechanisms are major current challenges in this field.It is evident that diverse signaling molecules can be produced or derived from bacteria and plants,and researchers have sought to identify these signals and explore the mechanisms of the signaling pathways.The results of such studies will lead to the development of strategies to improve plant disease resistance through controlling interkingdom signals,rather than directly killing the pathogenic bacteria.Also,the identification of signals produced by beneficial bacteria will be useful for agricultural applications.In this review,we summarize the recent progress of cross-kingdom interactions between plant and bacteria,and how LuxR-family transcription factors in plant associated bacterial quorum sensing system are involved in the interkingdom signaling. | Jinhong Kan Rongxiang Fang Yantao Jia | 2017 | Science China(Life Sciences)2017，60，8： | 7 |
| 3 | XerR, a negative regulator of XccR in Xanthomonas campestris pv. campestris, relieves its repressor function in planta显示文摘我们以前报导了那 XccR, Xanthomonas campestris pv 的一个 LuxR 类型管理者。campestris (Xcc ) ,响应某些主人植物因素激活下游的脯氨酸 iminopeptidase 毒力基因(果仁) 。在这份报告,我们进一步证明 xccR 基因的表示被一个 NtrC 类型反应管理者在文化媒介镇压,我们把它称为 XerR (XccR 表示相关,抑压者) ,并且当细菌是时,这压抑被减轻,这在 planta 成长。如此的规章的机制被 XerR 直接绑了在 xccR 倡导者的观察增强在里面 vitro,和那在 XerR 的 phosphorylation 相关的残余的变化导致了它的抑压者功能的损失。而且,当他们是渗入进卷心菜植物的真空时, xccR 的表示水平甚至在 XerR-overexpressing Xcc 房间增加了。我们 preliminarily 也描绘了涉及在 Xcc 和主人植物之间的上述提及的相互作用的主人因素,显示出那有不到 1 kDa 废除了的分子的重量的植物材料到 xccR 倡导者的 XerR 的绑定,当一样的材料在果仁倡导者提高了 XccR 的绑定到 luxXc 框时。一起拿,我们的结果含有在控制毒力相关的 xccR/pip 地点的表示的规章的机制的新层的 XerR 并且提供线索给与 XerR 和 XccR 交往提高 Xcc 的毒力的植物信号分子的鉴定。 | Li Wang Lili Zhang Yunfeng Geng Wei Xi Rongxiang Fang Yantao Jia | 2011 | Cell Research2011，21，7： | 5 |
| 4 | Small G Protein as a Novel Component of the Rice Bmssinosteroid Signal Transduction显示文摘Brassinosteroids (BR ) 是为植物生长和开发是必要的类固醇荷尔蒙的一个班。在 dicot 模型植物 Arabidopsis 的 BR 信号 transduction 小径很好被建立,但是连接在米饭表明步的 BR 的部件一直不是充分探索了。例如,特别在 BR 受体水平,发信号的 BR 怎么在米饭被调整,大部分是未知的。这里,我们显示出那 OsPRA2,米饭小 G 蛋白质,在发信号的 BR 起一个压抑的作用小径。薄板倾斜, coleoptile 延伸,和根抑制试金显示有 OsPRA2 的压制的表示的米饭植物比野类型的植物对外长地应用的 brassinolide 更敏感。相反地,米饭 overexpressing OsPRA2 对外长的 brassinolide 不太敏感。进一步的学习揭开了那 OsPRA2 禁止了 dephosphorylation,并且因此使抄写因素失去活性 BRASSINAZOLE 抵抗 1 (OsBZR1 ) 。更重要地, OsPRA2 被发现到共同本地化与并且直接绑在米饭 BR 受体 BRASSINOSTEROID 感觉迟钝 1 (OsBRI1 ) 在血浆膜。另外,在里面 vitro 试金证明 OsPRA2 禁止它的 autophosphorylation。这个 OsPRA2-OsBRI1 相互作用从它的合作受体 OsBAK1 导致了 OsBRI1 的分离,并且废除了 OsBAK1 的调停 OsBRI1 的 phosphorylation。一起,这些结果在 OsBRI1 和 OsBZR1 上作为一个新奇否定管理者揭示 OsPRA2 的可能的工作机制并且关于米饭 BR 发信号的规章的机制扩大知识。 | Ge Zhang Xiaoguang Song Hongyan Guo Yao Wu Xiaoying Chen Rongxiang Fang | 2016 | Molecular Plant2016，9，9： | 5 |
| 5 | SRWD1, a novel target gene of DELLA and WRKY proteins, participates in the development and immune response of rice (Oryza sativa L.)显示文摘SRWD1, a member of the WD40 protein subfamily, is induced by salt stress in rice and its homolog in barley can bind to GAMYB, implying that SRWD1 might be involved in plant defense against environmental stress and gibberellic acid(GA) signalings. In this study, we focused on the biological functions and regulation mechanisms of SRWD1 in rice. The results showed that SRWD1 expression was repressed by GA and induced by abscisic acid(ABA). Two WRKY-family transcription factors, Os WRKY45 and Os WRKY72, were found to regulate SRWD1 expression by directly binding to the W-box region in its promoter. Transient co-expression and yeast two-hybrid analyses showed that a DELLA protein strengthened the activation of Os WRKY45 and partly relieved the suppression of Os WRKY72 by binding to them.Interestingly, both SRWD1-overexpressing transgenic plants and SRWD1-knockout mutants showed dwarf phenotypes and resistance to Xanthomonas oryzae. | Chuanmiao Xie Ge Zhang Yuman Zhang Xiaoguang Song Hongyan Guo Xiaoying Chen Rongxiang Fang | 2017 | Science Bulletin2017，62，24： | 4 |
| 6 | Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants显示文摘The human pathogen, group A rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. There is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. Plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. Virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live attenuated viral vaccines. In this study, the rotavirus capsid proteins VP2, VP6 and VP7 were co-expressed in transgenic tobacco plants, and their expression levels, formation of rotavirus-like particles (RV VLPs) and immunogenicity were extensively studied. Quantitative real-time RT-PCR and Western blot analysis revealed that the expression level of vp6 was the highest while vp7 was expressed at the lowest levels. The RV VLPs were purified from transgenic tobacco plants and analyzed by electron microscopy and Western blot. Results indicated that the plant-derived VP2, VP6 and VP7 proteins self-assembled into 2/6 or 2/6/7 RV VLPs with a diameter of 60-80 nm. When orally delivered into mice with cholera toxin as an adjuvant, the total soluble protein extracted from transgenic tobacco plants induced rotavirus-specific antibodies comparable with those of attenuated rotavirus vaccines, while VP 2/6/7 induced higher serum IgG and fecal IgA titers compared with VP 2/6. | YANG YanMei LI Xia YANG Hui QIAN Yuan ZHANG You FANG RongXiang CHEN XiaoYing | 2011 | Science China(Life Sciences)2011，54，1： | 4 |
| 7 | The Rice'NUTRITION RESPONSE AND ROOT GROWTH' (NRR) Gene Regulates Heading Date显示文摘Dear Editor, As one of the most important developmental traits,flowering time has been extensively investigated in the long-day plant Arabidopsis.Multiple factors are involved,including photoperiod and/or temperature (Amasino,2010).Flowering locus T (FT) is known to be an important integrator of different flowering pathways (Taoka et al.,2011).Genes of the CONSTANS (CO) family also play key roles in regulating flowering time.The CO protein consists of a zinc finger and a CCT domain shared by a group of plant-specific transcription factors that regulate photoperiodic flowering response and circadian rhythms in Arabidopsis.In the case of short-day plants such as rice,previous studies have also revealed a number of genes that control heading date,such as the CO ortholog Heading date 1 (Hdl) and,at its downstream,Heading date 3a (Hd3a),an ortholog of FT (Kojima et al.,2002;Komiya et al.,2008).Although the main photoperiodic flowering regulators are highly conserved between Arabidopsis and rice,there are genes that act differently relating to the daylength property of rice,such as Early heading datel (Ehdl),Indeterminate1 (OslD1),the MADS-box gene OsMADS51 (Doiet al.,2004;Kim et al.,2007;Park et al.,2008),and Ghd7 (Xue et al.,2008). | Yuman Zhang Ge Zhang Na Xiao Lina Wang Yaping Fu Zongxiu Sun Rongxiang Fang Xiaoying Chen | 2013 | Molecular Plant2013，6，2： | 3 |
| 8 | Designing specific bacterial 16S primers to sequence and quantitate plant endo-bacteriome显示文摘Plant endophytic bacteria colonize the internal tissues of plants and interact with plants closely.The past two decades have witnessed the increasing application of next-generation 16S r RNA gene sequencing in the investigation of bacterial communities.However,deciphering plant endo-bacterial communities by this method is difficult because of the co-amplification of massive plant organellar DNAs with bacterial 16S.Here,we designed polymerase chain reaction(PCR) primer sets,including799F/1107R,322F/796R,and 322F-Dr/796Rs(primer pair 322F/796R with a penultimate-base substitution in 322F),that can specifically amplify bacterial 16S from plant total DNAs.We computationally and experimentally evaluated the specificity,coverage,and accuracy of the newly designed primer sets.Both 799F/1107R and 322F-Dr/796Rs produced plant DNA-free 16S amplicon libraries or reduced plant DNA contamination to lower than 5% for the plant materials with extremely-low-abundance bacterial communities.The primer set 322F-A/796R was used through absolute quantitative PCR to quantitate the population size of rice leaf or root endo-bacteriome,which revealed 10^(6)–10^(7) and 10^(9)–10^(10) bacteria per gram fresh weight,respectively.These 16S primer sets and amplification methods enable the simple and inexpensive next-generation sequencing and quantification of plant endo-bacteriome,which will significantly advance studies on the plant-related microbiome. | Liying Chen Mengting Zhang Da Liu Hongbo Sun Jianxiang Wu Yan Huo Xiaoying Chen Rongxiang Fang Lili Zhang | 2022 | Science China(Life Sciences)2022，65，5： | 2 |
| 9 | Manipulation of biotic signaling:a new theory for smarter pest control显示文摘Modern agricultural bio-technologies have significantly increased the efficiency and quality of crop production and contributed to the improvement of people's life in the world.Widespread utilization of fertilizers,herbicides,fungicides,pesticides,antibiotics,and plant growth regulators has been instrumental in securing crop yields to feed over seven billions of human beings in the earth.However,during the recent half century,extensive uses of pesticides and other chemicals,which traditionally target the essential physiological pathways of pathogenic microbes and pests,have proverbial,negative impacts on the environmental | Wei Qian Xiaoya Chen Rongxiang Fang Le Kang | 2017 | Science China(Life Sciences)2017，60，8： | 2 |
| 10 | Removal of the Selectable Marker Gene from Transgenic Tobacco Plants by Expression of Cre Recombinase from a Tobacco Mosaic Virus Vector through Agroinfection显示文摘 | Hongge Jia Yongqi Pang Xiaoying Chen Rongxiang Fang | 2006 | Transgenic Research2006，，3： | 1 |
| 11 | Artificial microRNAs highly accessible to targets confer efficient virus resistante in plants显示文摘 | Duan Chengguo Warn Chunhan Fang Rongxiang | 2008 | J Virol2008，82，11： | 1 |
| 12 | Artificial rnicroRNA-mediated virus resistance in plants显示文摘 | Qu Jing Ye Jian Fang Rongxiang | 2007 | J Virol2007，81，： | 1 |
| 13 | Function of resveratrol derived from transgenic plant expressing resveratrol syn-thase gene显示文摘Two genes from grapevine coding for resvera-trol synthase, named RS1 and RS2, were cloned by RT-PCR. An Escherichia coli expression vector was constructed by insertion of RS1 into pBV221. A specific protein with the same molecular weight (42 ku) as the resveratrol synthase was expressed and used to prepare the rabbit antiserum. A plant expression vector was constructed by inserting the RS1 gene into pBin438 downstream of the doubled CaMV 35S promoter and TMV-Ω fragment. PCR-positive transgenic tobacco plants were obtained after transformation with Agrobacterium tumefaclens LBA4404 harboring the plant expression vector. Southern blot analysis demonstrated that the foreign gene was integrated into the tobacco genome. The results of RT-PCR and Western blot indicated that the RS1 gene was transcribed and expressed. Formation of resveratrol in transgenic tobacco was further determined by thin-layer chromatography of silica gel and HPLC. Increased accumulation of human breast adenocarcinoma cells in G0 and | La Ping Cai Wenqi Zhang Jiuchun Zhang Fengli Fang Rongxiang | 2001 | Chinese Science Bulletin2001，46，13： | 1 |
| 14 | RcSPL1-RcTAF15b regulates the flowering time of rose (Rosa chinensis)显示文摘Rose(Rosa chinensis),which is an economically valuable floral species worldwide,has three types,namely once-flowering(OF),occasional or re-blooming(OR),and recurrent or continuous flowering(CF).However,the mechanism underlying the effect of the age pathway on the duration of the CF or OF juvenile phase is largely unknown.In this study,we observed that the RcSPL1 transcript levels were substantially upregulated during the floral development period in CF and OF plants.Additionally,accumulation of RcSPL1 protein was controlled by rch-miR156.The ectopic expression of RcSPL1 in Arabidopsis thaliana accelerated the vegetative phase transition and flowering.Furthermore,the transient overexpression of RcSPL1 in rose plants accelerated flowering,whereas silencing of RcSPL1 had the opposite phenotype.Accordingly,the transcription levels of floral meristem identity genes(APETALA1,FRUITFULL,and LEAFY)were significantly affected by the changes in RcSPL1 expression.RcTAF15b protein,which is an autonomous pathway protein,was revealed to interact with RcSPL1.The silencing and overexpression of RcTAF15b in rose plants led to delayed and accelerated flowering,respectively.Collectively,the study findings imply that RcSPL1–RcTAF15b modulates the flowering time of rose plants. | Rui Yu Zhiying Xiong Xinhui Zhu Panpan Feng Ziyi Hu Rongxiang Fang Yuman Zhang Qinglin Liu | 2023 | Horticulture Research2023，10，6： | 0 |
| 15 | Microbe-induced gene silencing explores interspecies RNAi and opens up possibilities of crop protection显示文摘In most eukaryotes, small RNA(sRNA)-mediated RNA silencing(or RNA interference, RNAi) is an evolutionarily conserved mechanism that regulates gene expression in a sequence-specific manner.Generally, s RNAs are produced by the RNase III enzymes Dicers and then incorporated into Argonaute(AGO) proteins to regulate gene expression by mRNA degradation. | Rongxiang Fang | 2024 | Science China(Life Sciences)2024，67，3： | 0 |
| 16 | Support vector regression-based study of interference in absorption spectral lines of mixed gases显示文摘When measuring the concentration of multi-component gas mixtures based on supercontinuum laser absorption spectroscopy(SCLAS), there are interferences between the absorption spectral lines. For the spectral interference problem of CO_(2) and CH4 at 1 432 nm, a method based on support vector regression(SVR) is proposed in this paper. The SVR model, the k-nearest neighbor(KNN) model and the least squares(LS) model are used to analyze and predict the absorption spectral data, and the prediction accuracies were 96.29%, 88.89% and 85.19%, respectively, with the highest prediction accuracy of the SVR model. The results show that the method can accurately measure the concentration of gas mixtures, realize the detection of mixed gases using a single waveband, and provide a solution to the overlapping spectral line interference of multi-component gas mixtures. | YAN Xiangyu LI Honglian WANG Yitong FANG Lide ZHANG Rongxiang | 2022 | Optoelectronics Letters2022，18，12： | 0 |
| 17 | RoTFL1c of Rosa multiflora has a dual-function in suppressing reproductive growth and promoting vegetative growth of Arabidopsis显示文摘Dear Editor, TFL1homologues play a key role in the flowering habit of roses.To analyze the function of the newly isolated TFL1 gene of Rosa multiflora(Chen et al.,2013),named RoTFLlc,we performed overexpression analyses in Arabidopsis thaliana.When compared with empty vector control and wild type Arabidopsis plants,RoTFLlc overexpressing transgenic plants exhibited strong phenotypes such as a clustered habit,an increased number of rosette leaves, late flowering or failure to flower. | Fang Wang Lijuan Lian Yunfeng Liu Yuman Zhang Rongxiang Fang Qinglin Liu | 2018 | Science China(Life Sciences)2018，61，12： | 0 |
| 18 | Heterosis in root microbiota inhibits growth of soil-borne fungal pathogens in hybrid rice显示文摘In nature,plants are colonized by various microbes that play essential roles in their growth and health.Heterosis is a natural genetic phenomenon whereby first-generation hybrids exhibit superior phenotypic performance relative to their parents.It remains unclear whether this concept can be extended to the“hybridization”of microbiota from two parents in their descendants and what benefits the hybrid microbiota might convey.Here,we investigated the structure and function of the root microbiota from three hybrid rice varieties and their parents through amplicon sequencing analysis of bacterial 16S ribosomal DNA(rDNA)and fungal internal transcribed spacer(ITS)regions.We show that the bacterial and fungal root microbiota of the varieties are distinct from those of their parental lines and exhibit potential heterosis features in diversity and composition.Moreover,the root bacterial microbiota of hybrid variety LYP9 protects rice against soil-borne fungal pathogens.Systematic analysis of the protective capabilities of individual strains from a 30-member bacterial synthetic community derived from LYP9 roots indicated that community members have additive protective roles.Global transcription profiling analyses suggested that LYP9 root bacterial microbiota activate rice reactive oxygen species production and cell wall biogenesis,contributing to heterosis for protection.In addition,we demonstrate that the protection conferred by the LYP9 root microbiota is transferable to neighboring plants,potentially explaining the observed hybrid-mediated superior effects of mixed planting.Our findings suggest that some hybrids exhibit heterosis in their microbiota composition that promotes plant health,highlighting the potential for microbiota heterosis in breeding hybrid crops. | Mengting Zhang Yinyue Wang Yuanyi Hu Huacai Wang Yawen Liu Bingran Zhao Jie Zhang Rongxiang Fang Yongsheng Yan | 2023 | Journal of Integrative Plant Biology2023，65，4： | 0 |
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