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| 1 | 四种心肌克隆钾通道的表达和电生理记录显示文摘为建立一种研究离子通道的有效模型 ,笔者应用分子生物学技术 ,将包含有Kv1.4、Kv1.5、Herg和KvLQT1等几种心肌克隆钾离子 (K+ )通道cDNA的质粒经扩增 ,提取 ,以及纯化后转录为mRNA。经微注射技术分别将它们注射入克隆离子通道表达系统———非洲爪蟾卵母细胞内 ,然后应用膜片钳技术记录上述克隆离子通道电流。结果 :获得了四种克隆通道的电流 时间关系曲线 (I/t)和电流 电压关系曲线 (I/V)。结论 :本实验结果证明所采用的技术稳定可靠 ,并为今后开展克隆离子通道结构和功能的关系以及抗心律失常药物与离子通道相互作用的研究提供了坚实的基础。 | 徐林 黄从新 蒋学俊 李晓艳 吴钢 唐哨勇 Duffey M Strauss HC Rasmusson RL 王世敏 | 2004 | 中国心脏起搏与心电生理杂志2004，18，2： | 6 |
| 2 | Mesophilic aeromonads in human disease:current taxonomy laboratory identification and infectious disease spectrum显示文摘 | Janda J M Duffey P S | 1988 | Rev Infect Dis1988，10，： | 1 |
| 3 | Military movement training program improves jump-landing mechanics associated with anterior crueiate ligament injury risk 显示文摘 | Owens B D Cameron K L Duffey M L | 2012 | Journal of surgical orthopaedic advances2012，22，1： | 1 |
| 4 | An Evidence-based Project for Evaluating Strategies to Improve Knowledge Acquisition and Critical-thinking Performance in Nursing Students显示文摘 | Angel B F Duffey M Belyea M | 2000 | J Nurs Educ2000，39，5： | 1 |
| 5 | Pathoanatomy of rst-time, traumatic, anterior glenohumeral subluxation events显示文摘 | OWENS B D NELSON B J DUFFEY M L | 2010 | J Bone Joint Surg Am2010，92，： | 1 |
| 6 | Mesophilic aeronaonads in hu-man disease: current taxonomy,laboratory identifica-tion, and infectious disease spectrum 显示文摘 | Janda J M Duffey P S | 1988 | Rev InfectDis1988，105，： | 1 |
| 7 | An evidence-based project for evaluating strategies to improve knowledge acquisition and critical-thinking performance in nursing students显示文摘 | Angel B F Duffey M Belyea M | 2000 | J Nurs Educ2000，39，5： | 1 |
| 8 | An evidence - based project for e- valuating strategies to improve knowledge acquisition and critical - thinking performance in nursing students 显示文摘 | Angel BF Duffey M Belyea M | 2000 | J Nurs Educ2000，39，5： | 1 |
| 9 | Experimental verification of scaling laws for punch-impact-loaded strutures显示文摘 | DUFFEY T A CHERESH M C | 1984 | International Journal of Impact Engineering1984，，2： | 1 |
| 10 | An evidence--based project for evaluating strategies to improve knowledge acquisition and critical thinking performance in nursing students显示文摘 | Angel BF Duffey M Belyea M | 2000 | J Nurs Educ2000，39，5： | 1 |
| 11 | Mesophilic aeromonads in human disease: current taxonomy laboratory identification and infectious disease spectrum显示文摘 | Janda J M Duffey P S | 1988 | Rev Infect Dis1988，10，： | 1 |
| 12 | A model for effective implementation of earned value management methodology显示文摘 | Kim E Well W G Duffey M R | 2003 | Int J of Project Management2003，21，5： | 1 |
| 13 | Identity, spa- tial distribution, and variability of induced chemical responses in tomato plants 显示文摘 | Stout M J Workman K V Duffey S S | 1996 | Ento-mologia experimentalis et applicata1996，79，3： | 1 |
| 14 | The effect of dietary protein on the growth and digestive physiology of larval Heliothis zea and Spodoptera eccigus 显示文摘 | Broadway R M Duffey S S J | 1986 | Insect Physiol1986，32，8： | 1 |
| 15 | Exogenous jasmonates simulate insect wounding in tomato plants(Lycopersicon esculentum)in the laboratory and field显示文摘 | Thaler JS Stout M J Karban R Duffey SS | | 0，，10： | 1 |
| 16 | Plant proteinase inhibitors mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua显示文摘 | Broadway R M Duffey S S | 1986 | Insect Physiol1986，32，10： | 1 |
| 17 | Identity, spatial distribution and variability of induced chemical responses in tomato plants 显示文摘 | STOUT M J WORKMAN K V DUFFEY S S | 1996 | Entomol Exp App1996，79，： | 1 |
| 18 | Mesophilic aeromonads in humandisease: current taxonomy laboratory identification and infectious disease spectrum显示文摘 | JANDA J M DUFFEY P S | 1988 | Rev Infect Dis1988，10，： | 1 |
| 19 | An evidence--based projeefor evaluat- ing strategies to improve knowledge acquisitionand critical thinking perfor- mance in nursing students显示文摘 | Angel B F Duffey M Belyea M | 2000 | JNurs Educ2000，39，5： | 1 |
| 20 | Plant proteinase inhibitors,Mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua 显示文摘 | BROADWAY R M DUFFEY S S | 1986 | J Insect Physiol1986，32，： | 1 |
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