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| 1 | Hepatitis B and C virus-induced hepatitis: Apoptosis, autophagy, and unfolded protein response显示文摘AIM: To investigate the co-incidence of apoptosis, autophagy, and unfolded protein response(UPR) in hepatitis B(HBV) and C(HCV) infected hepatocytes.METHODS: We performed immunofluorescence confocal microscopy on 10 liver biopsies from HBV and HCV patients and tissue microarrays of HBV positive liver samples. We used specific antibodies for LC3β, cleaved caspase-3, BIP(GRP78), and XBP1 to detect autophagy, apoptosis and UPR, respectively. AntiHCV NS3 and anti-HBs antibodies were also used to confirm infection. We performed triple blind counting of events to determine the co-incidence of autophagy(LC3β punctuate), apoptosis(cleaved caspase-3), and unfolded protein response(GRP78) with HBV and HCV infection in hepatocytes. All statistical analyses were performed using SPSS software for Windows(Version 16 SPSS Inc, Chicago, IL, United States). P-values < 0.05 were considered statistically significant. Statistical analyses were performed with Mann-Whitney test to compare incidence rates for autophagy, apoptosis, and UPR in HBV- and HCV-infected cells and adjacent noninfected cells.RESULTS: Our results showed that infection of hepatocytes with either HBV and HCV induces significant increase(P < 0.001) in apoptosis(cleavage of caspase-3), autophagy(LC3β punctate), and UPR(increase in GRP78 expression) in the HCV- and HBVinfected cells, as compared to non-infected cells of the same biopsy sections. Our tissue microarray immunohistochemical expression analysis of LC3β in HBV^(Neg) and HBV^(Pos) revealed that majority of HBVinfected hepatocytes display strong positive stainingfor LC3β. Interestingly, although XBP splicing in HBVinfected cells was significantly higher(P < 0.05), our analyses show a slight increase of XBP splicing was in HCV-infected cells(P > 0.05). Furthermore, our evaluation of patients with HBV and HCV infection based on stage and grade of the liver diseases revealed no correlation between these pathological findings and induction of apoptosis, autophagy, and UPR.CONCLUSION: The results of this study indicate that HCV and HBV infection activates apoptosis, autophagy and UPR, but slightly differently by each virus. Further studies are warranted to elucidate the interconnections between these pathways in relation to pathology of HCV and HBV in the liver tissue. | Behzad Yeganeh Adel Rezaei Moghadam Javad Alizadeh Emilia Wiechec Seyed Moayed Alavian Mohammad Hashemi Bita Geramizadeh Afshin Samali Kamran Bagheri Lankarani Martin Post Payam Peymani Kevin M Coombs Saeid Ghavami | 2015 | World Journal of Gastroenterology2015，21，47： | 13 |
| 2 | 对明串珠菌及其他细菌的抑制效果显示文摘在从压榨间直到蔗汁澄清的蔗糖生产过程中,蔗汁中微生物活动是破坏蔗糖品质的一个重要因素。蔗糖损失和葡聚糖的形成通常与蔗汁中微生物引起的变质有关。多年来,这一直成为糖厂试图改善蔗糖品质时所面临的问题。本文以直接影响得糖率的微生物减少和相对纯度为指标,研究了不同杀菌方法在蔗汁中的效果。浓度为0.007%的S抑制剂显示了对某糖厂初压汁和混合汁中的微生物菌群的抗菌效应。在对照组中,12h后观察到4.8单位的纯度降低。加入S抑制剂后,只降低2个单位。且蔗汁的稳定性在3个样品中几乎完全一致。以S抑制剂处理的蔗汁保持深褐色和清新的气味,且pH与初始相比无显著变化,而未处理的蔗汁变浅褐色,产生很强的酒味,pH和纯度显著降低。这种稳定效应尚未被其他常用的糖用杀菌剂所报导。同样,本文证明了有可能通过使用S抑制剂来稳定混合汁。经S抑制剂处理后,游离的还原物质和多聚糖比不经处理的样品低6倍。这些结果表明,形成的葡聚糖以及随之形成的游离果糖减少。 | Georgina Michelena Aidin Martinez Graciela Cerutti Monica Coronel Antonio Bell Emilia Carrera Agustin Lopez-Murguia Roberto Portuondo Lazaro Mergarejo Frost M Steele 李宜海 | 2006 | 广西轻工业2006，22，4： | 3 |
| 3 | Efficacy of Milsana *,a formulated plant extract from Reynoutria sachalinensis,against powdery mildew of tomato (Leveillula taurica)显示文摘 | Koostantinidou-Doltsinis S Emilia Markellou Kasselaki A M | 2006 | BioControl2006，51，： | 1 |
| 4 | Helicobacter pylori eradication can induce platelet recovery in idiopathic thrombocytopenic purpura显示文摘 | Emilia G Longo G Luppi M | 2001 | Blood2001，97，3： | 1 |
| 5 | Residual C-peptide secretion and endothelial function in patients with type 2 diabetes显示文摘 | Daniela M Emilia R Angela MA | 2003 | Clinical Science2003，105，1： | 1 |
| 6 | Evaluation of induction of systemic resistance in pepper plants (Capsicum annum) to Phytophtora capsici using Trichoderma harzianum and its relation with capsidiol accumulation 显示文摘 | Perez S C Emilia C M | 2000 | European Journal of Plant Pathology2000，106，9： | 1 |
| 7 | Residual C-peptide secretion and endothelial function in patients with type2 diabetes显示文摘 | Daniela M Emilia R Angela Marie A | 2003 | Clinical Science2003，105，： | 1 |
| 8 | Storage quality of fresh and frozen- thawed fish in ice 显示文摘 | Hannes M Emilia M | 1995 | Journal of Food Science1995，60，2： | 1 |
| 9 | A rapid and simple procedure for the determination of synephrine in dietary supplements by gas chromatography-mass spectrometry显示文摘 | Emilia M Simona P Roberta P et ol | 2006 | J Pharmaceut Biomed2006，41，： | 1 |
| 10 | Evaluation of induction of systemic resistance in pepper plants ( Capsicum annum ) to Phytophtora capsici using Trichoderma harzianum and its relation with capsidiol accumulation 显示文摘 | Ahmed S A Perez S C Emilia C M | 2000 | European Journal of Plant Pathology2000，106，9： | 1 |
| 11 | Randomized trial of non-invasive ventilation combined with exercise training in patients with chronic hypercapnic failure due to Chronic Obstructive Pulmonary Disease显示文摘 | Eduardo Márquez-Martín Francisco Ortega Ruiz Pilar Cejudo Ramos Jose Luis López-Campos Borja Valencia Azcona Emilia Barrot Cortés | 2014 | Respiratory Medicine2014，，： | 1 |
| 12 | Long-termsalvage therapy with cyclosporine A in refractory idiopathic thrombocytopenic purpura显示文摘 | Emilia G Morselli M Luppi M | 2002 | Blood2002，99，4： | 1 |
| 13 | Purification,Partial characterization and mode of action of enterococcin EFS2,an antilisterial bacteriocin produced by a swain of Enterococcusfaecalis isolation from a cheese显示文摘 | Sophie M P Emilia F Richard J | 1996 | International Journal of Food Microbiology1996，30，： | 1 |
| 14 | A possible role for low-dose cyclosporine in refractory immune thrombocytopenic purpura显示文摘 | Emilia G Luppi M Morselli M | 2008 | Haematol2008，93，7： | 1 |
| 15 | helicobacter pylori eradication can induce platelet recovery in idiopathic thrombocytopenic purpura显示文摘 | Emilia G Longo G Luppi M | 2001 | Blood2001，97，3： | 1 |
| 16 | A possible role for low-dose cyclosporine in refractory immune thrombocytopenic purpura显示文摘 | Emilia G Luppi M Morselli M | | 0，，07： | 1 |
| 17 | Effect directed isolation and identification of antibacterial Chelidonium majus L alkaloids显示文摘 | 6gnes M M6ricz Emilia Fornal Wioleta Jesionek | 2015 | Chromatographia2015，78，： | 1 |
| 18 | Helicobacter pylori eradication can induce platelet recovery in idiopathic thrombocytopenic purpura显示文摘 | Emilia G Longo G Luppi M | 2001 | Blood2001，97，： | 1 |
| 19 | Synthesis of peptide dendrimers based on a β-cyclodextrin core with guest binding ability 显示文摘 | Abdullah M A Muhanna Emilia Ortiz-Salmeron Luis Garcia-Fuentes | 2003 | Tetrahedron Lett2003，44，32： | 1 |
| 20 | Infectious agents and hu- man immune diseases: Lessons from Helieobacter pylori 显示文摘 | Emilia G Luppi M Torelli G | 2005 | Am J Med2005，118，4： | 1 |
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