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| 1 | Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation显示文摘AIM:To provide further insight into the characterization of mucosa-associated Escherichia coli(E.coli)isolated from the colonic mucosa of cancer patients.METHODS:Phylogroups and the presence of cyclomodulin-encoding genes of mucosa-associated E.coli from colon cancer and diverticulosis specimens weredetermined by PCR.Adhesion and invasion experiments were performed with I-407 intestinal epithelial cells using gentamicin protection assay.Carcinoembryonic antigen-related cell adhesion molecule 6(CEACAM6)expression in T84 intestinal epithelial cells was measured by enzyme-linked immunosorbent assay and by Western Blot.Gut colonization,inflammation and procarcinogenic potential were assessed in a chronic infection model using CEABAC10 transgenic mice.Cell proliferation was analyzed by real-time mRNA quantification of PCNA and immunohistochemistry staining of Ki67.RESULTS:Analysis of mucosa-associated E.coli from colon cancer and diverticulosis specimens showed that whatever the origin of the E.coli strains,86%of cyclomodulin-positive E.coli belonged to B2 phylogroup and most harbored polyketide synthase(pks)island,which encodes colibactin,and/or cytotoxic necrotizing factor(cnf)genes.In vitro assays using I-407 intestinal epithelial cells revealed that mucosa-associated B2 E.coli strains were poorly adherent and invasive.However,mucosa-associated B2 E.coli similarly to Crohn’s disease-associated E.coli are able to induce CEACAM6expression in T84 intestinal epithelial cells.In addition,in vivo experiments using a chronic infection model of CEACAM6 expressing mice showed that B2 E.coli strain11G5 isolated from colon cancer is able to highly persist in the gut,and to induce colon inflammation,epithelial damages and cell proliferation.CONCLUSION:In conclusion,these data bring new insights into the ability of E.coli isolated from patients with colon cancer to establish persistent colonization,exacerbate inflammation and trigger carcinogenesis. | Jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre Sauvanet Emilie Vazeille Amélie de Vallée Pierre Déchelotte Claude Darcha Denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette Darfeuille-Michaud | 2014 | World Journal of Gastroenterology2014，20，21： | 12 |
| 2 | 在在母亲胎儿的接口的天生、适应的有免疫力的房间之间的不平衡发生在导致内毒素的 preterm 出生以前显示文摘Preterm 出生(PTB ) 是新生的病态和世界范围的死亡的领先的原因。到在母亲并且在母亲胎儿的接口的一个支持 inflammatory 状态的来自一个反煽动性的状态的转变在微生物引起导致 preterm 劳动的 pathophysiology 被含有。然而,房间哪个免疫者调停,是不清楚的这转变。我们假设了那在在母亲胎儿的接口的天生、适应的有免疫力的房间之间的不平衡将发生在微生物引起导致 preterm 劳动以前。用导致内毒素的 PTB 的一个确定的鼠科的模型,我们的结果证明在交货以前,有 CD4+ 的减小;在子宫的纸巾的规章的 T 房间(Tregs ) 。这减小被子宫的纸巾也不在脾连接到 Tregs 的一个减少的数字,也不到 IL10, CCL17,或 CCL22 的损害生产。到怀孕老鼠的内毒素管理不改变受动器 CD4+在母亲胎儿的接口的 T 房间。然而,它引起在 Tregs 之间的不平衡(CD4+并且 CD8+) ,受动器 CD8+在怒气的 T 房间,和 Th17 房间。另外,到怀孕老鼠的内毒素管理象丰富的 neutrophils 一样由子宫的纸巾导致 CCL2, CCL3, CCL17,和 CCL22 的过多的生产。在子宫的微型环境的这不平衡被象巨噬细胞和 MHC II+ 那样的少见的象 APC 一样房间伴随;neutrophils。一起,这些结果证明到怀孕老鼠的内毒素管理在母亲胎儿的接口引起在天生、适应的有免疫力的房间之间的不平衡。 | Marcia Arenas-Hernandez Roberto Romero Derek St Louis Sonia S Hassan Emily B Kaye Nardhy Gomez-Lopez | 2016 | Cellular & Molecular Immunology2016，13，4： | 6 |
| 3 | 间接免疫荧光试验在检测风疹病毒感染中的应用显示文摘目的建立检测风疹病毒(RV)抗原E1、E2、C的间接免疫荧光试验(IFA),为快速、敏感地检测临床标本中的RV提供一种重要的检测手段。方法采用荧光素标记的羊抗鼠IgG抗体,检测病毒抗原与抗风疹病毒衣壳蛋白E1、E2、C蛋白的单克隆抗体的结合物。用该系统测定中国麻疹实验室网络送检的咽拭子标本,并与病毒分离结果和临床诊断进行比较。结果IFA方法可以快速、敏感地检测临床标本中的RV。经检测咽拭子标本38份,其中28份采集于临床诊断风疹病例;6例采集于出疹患者,但诊断不明;4份采集于风疹病例的接触者。检测结果有22份风疹阳性标本。IFA的结果与病毒分离结果完全一致,但与临床诊断不一致,IFA结果的阳性数低于临床诊断风疹病例数。结论IFA方法简单可行、敏感特异,结果易于判断,是中国麻疹实验室网络中进行RV检测的重要方法之一。同时IFA方法也可以作为临床快速诊断RV感染的备选方法之一。 | 朱贞 许文波 李聪勇 Emily S Abernathy 周淑洁 王常银 张珍英 Joseph Icenogle | 2005 | 中国计划免疫2005，11，5： | 5 |
| 4 | Cortical laminar necrosis related to migrainous cerebral infarction显示文摘We present a 29-year-old woman with a long history of attacks of migraine with and without visual aura.She was a heavy smoker(20 cigarettes/d) and was currently taking oral contraceptives. During a typical migraine attack with aura, she developed dysarthria,left brachial hemiparesis and hemihypoesthesia and brief and autolimited left clonic facial movements. Four hours after onset, vascular headache and focal sensorimotor neurological deficit were the only persisting symptoms and, on seventh day, she was completely recovered. Brain magnetic resonance imaging on day 20 after onset showed a subacute ischemic lesion in the right temporo-parietal cortex compatible with cortical laminar necrosis(CLN). Extensive neurological work-up done to rule out other known causes of cerebral infarct with CLN was unrevealing. Only ten of 3.808 consecutive stroke patients included in our stroke registry over a 19-year period fulfilled the strictly defined International Headache Society criteria for migrainous stroke. The present case is the unique one in our stroke registry that presents CLN related to migrainous cerebral infarction. Migrainous infarction can result in CLN. | Adrià Arboix Sebastià González-Peris Elisenda Grivé María-José Sánchez Emili Comes | 2013 | World Journal of Clinical Cases2013，1，8： | 4 |
| 5 | Aberrant Th2 inflammation drives dysfunction of alveolar macrophages and susceptibility to bacterial pneumonia显示文摘The ubiquitin ligase,Itch,is required to prevent autoinflammatory disease in mice and humans.Itch-deficient mice develop lethal pulmonary inflammation characterized by the production of Th2 cytokines(for example,interleukin-4(IL-4));however,the contribution of Itch to immune defense against respiratory pathogens has not been determined.We found that Itch-deficient mice were highly susceptible to intranasal infection with the respiratory pathogen Klebsiella pneumoniae.Infected Itch-deficient mice exhibited increased immune cell infiltration,cytokine levels and bacterial burden in the respiratory tract compared with control mice.However,numbers of resident alveolar macrophages were reduced in the lungs from Itch-deficient mice both before and after infection.High levels of Th2 cytokines in the respiratory tract correlated with deceased alveolar macrophages,and genetic ablation of IL-4 restored alveolar macrophages and host defense to K.pneumoniae in Itch-deficient mice,suggesting that loss of alveolar macrophages occurred as a consequence of Th2 inflammation.Adoptive transfer of Itch−/−CD4+T cells into Rag−/−mice was sufficient to drive reduction in numbers of Itch-replete alveolar macrophages.Finally,we found that Stat6 signaling downstream of the IL-4 receptor directly reduced fitness of alveolar macrophages when these cells were exposed to the Itch−/−inflamed respiratory tract.These data suggest that Th2 inflammation directly impairs alveolar macrophage fitness in Itch−/−mice,and elucidate a previously unappreciated link between Th2 cells,alveolar macrophages and susceptibility to bacterial infection. | Emily K Moser Natania S Field Paula M Oliver | 2018 | Cellular & Molecular Immunology2018，15，5： | 2 |
| 6 | Spinal alignment evolution with age:A prospective gait analysis study显示文摘AIM To describe,using gait analysis,the development of spinal motion in the growing child.METHODS Thirty-six healthy children aged from 3 to 16 years old were included in this study for a gait analysis(9m-walk).Various kinematic parameters were recorded and analyzed such as thoracic angle(TA),lumbar angle(LA)and sagittal vertical axis(SVA).The kinetic parameters were the net reaction moments(N.m/kg)at the thoracolumbar and lumbosacral junctions.RESULTS TA and LA curves were not statistically correlated to the age(respectively,P=0.32 and P=0.41).SVA increased significantly with age(P<0.001).Moments in sagittal plane at the lumbosacral junction were statistically correlated to the age(P=0.003),underlining the fact that sagittal mechanical constraints at the lumbosacral junction increase with age.Moments in transversal plane at the thoracolumbar and lumbosacral junctions were statistically correlated to the age(P=0.0002and P=0.0006),revealing that transversal mechanical constraints decrease with age.CONCLUSION The kinetic analysis showed that during growth,a decrease of torsional constraint occurs while an increase of sagittal constraint is observed.These changes in spine biomechanics are related to the crucial role of the trunk for bipedalism acquisition,allowing stabilization despite lower limbs immaturity.With the acquisition of mature gait,the spine will mainly undergo constraints in the sagittal plane. | Sébastien Pesenti Benjamin Blondel Emilie Peltier Elke Viehweger Vincent Pomero Guillaume Authier Stéphane Fuentes Jean-Luc Jouve | 2017 | World Journal of Orthopedics2017，8，3： | 2 |
| 7 | Morphology‐function relationships and repeatability in the sperm of Passer sparrows显示文摘 | Emily R.A. Cramer Terje Laskemoen Even Stensrud Melissah Rowe Fredrik Haas Jan T. Lifjeld Glenn‐Peter S?tre Arild Johnsen | 2015 | Journal of Morphology2015，，： | 2 |
| 8 | Zeldow, masculinity and fmininity pedict otimal nmtal halth: a belated test of the androgyny hypothesis显示文摘 | EMILY S L PETER B | 2006 | Jurnal of Prsonality Asment2006，870，： | 1 |
| 9 | NDRG1 Is Necessary for p53-dependent Apoptosis显示文摘 | Susanne S Emily K Thomas BH | 2004 | J Biol Chem2004，279，48： | 1 |
| 10 | Chemical composition of dissolved organic matter in agroecosystems~ Correlations with soil enzyme activity and carbon and nitrogen mineralization 显示文摘 | TIAN L EMILY D WEI S | 2010 | Applied Soil Ecology2010，46，： | 1 |
| 11 | Self-eare and qual- ity of life of heart failure patients at a multidisciplinary heart function clinic显示文摘 | Emily S Kevin J L Joseph A C | 2011 | J Cardiovase Nuts2011，26，5： | 1 |
| 12 | Antibacterial activity, inflammatory response, coagulation and cytotoxicity effects of silver nanoparticles显示文摘 | Fidel Martínez-Gutierrez Emily P. Thi Judith M. Silverman Carolina Camargo de Oliveira Sarah L. Svensson Amanda Vanden Hoek Elpidio Morales Sánchez Neil E. Reiner Erin C. Gaynor Edward L.G. Pryzdial Edward M. Conway Erasmo Orrantia Facundo Ruiz Yossef Av- | 2012 | Nanomedicine: Nanotechnology Biology and Medicine2012，，3： | 1 |
| 13 | Splicing and transcription-associated proteins PSF and p54nrb/NonO bind to the RNA polymeraseⅡ CTD显示文摘 | Emili A Shales M McCracken S | 2002 | RNA2002，8，9： | 1 |
| 14 | Career and life planning:Career development and counselling for young people显示文摘 | Leung S Emily Mee Mai | | 0，，10： | 1 |
| 15 | Management of multidrug-resistant organisms in healthcare settings,2006显示文摘 | Jane D S Emily R Marguerite J | 2007 | Am J Infect Control2007，35，102： | 1 |
| 16 | Plasma levels of soluble tumor necrosis factor receptors p55 and p75 in patients with alcoholic liver disease of increasing severity 显示文摘 | Naveau s Emilie D Balian A | 1998 | Hepatol1998，28，： | 1 |
| 17 | Addirg more beds to the emergency department or reduing admitted patient boarding times: which has a more significant influence onemergency department congestion 显示文摘 | Rahul K Khare Emilie S Powell Gilles Reinhardt | 2008 | Annals of Emergency Medicine2008，，7： | 1 |
| 18 | Differential radiometers using Fabry-Perot interferometric technique for remote sensing of greenhouse gases显示文摘 | E M Georgieva W S Heaps Emily L Wilson | 2008 | IEEE Transactions on Geoscienceand Remote sensing2008，46，10： | 1 |
| 19 | Presynaptic GABAA receptors enhance transmission and LTP induction at hippocampal mossy fiber synapses显示文摘 | Arnaud Ruiz Emilie Campanac Ricardo S Scott | 2010 | Nat Neurosci2010，13，4： | 1 |
| 20 | Initial Parathyroid Sur- gery in 606 Patients with Renal Hyperparathyroidism显示文摘 | Ralph S Emily PS Elias K | 2012 | World J Surg2012，36，2： | 1 |
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