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| 1 | Comprehensive and innovative techniques for livertransplantation in rats: A surgical guide显示文摘AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. | Tomohide Hori Justin H Nguyen Yasuhiro Ogura Toshiyuki Hata Shintaro Yagi Ann-Marie T Baine Norifumi Ohashi Christopher B Eckman Aimee R Herdt Hiroto Egawa Yasutsugu Takada Fumitaka Oike Seisuke Saka-moto Mureo Kasahara Kohei Ogawa Koichiro Hata Taku Iida Yukihide Yonekawa Lena Sibulesky Kagemasa Kuribayashi Takuma Kato Kanako Saito Mie Torii Naruhiko Sahara Naoko Kamo Tomoko Sahara Motohiko Yasutomi Shinji Uemoto | 2010 | World Journal of Gastroenterology2010，16，25： | 14 |
| 2 | Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice显示文摘AIM:To investigate the effect of matrix metalloproteinase-9(MMP-9) on the remnant liver after massive hepatectomy in the mouse.METHODS:Age-matched,C57BL/6 wild-type(WT),MMP-9(-/-),and tissue inhibitors of metalloproteinases(TIMP)-1(-/-) mice were used.The mice received 80%-partial hepatectomy(PH).Samples were obtained at 6 h after 80%-PH,and we used histology,immunohistochemical staining,western blotting analysis and zymography to investigate the effect of PH on MMP-9.The role of MMP-9 after PH was investigated using a monoclonal antibody and MMP inhibitor.RESULTS:We examined the remnant liver 6 h after 80%-PH and found that MMP-9 deficiency attenuated the formation of hemorrhage and necrosis.There were significantly fewer and smaller hemorrhagic and necrotic lesions in MMP-9(-/-) remnant livers compared with WT and TIMP-1(-/-) livers(P < 0.01),with no difference between WT and TIMP-1(-/-) mice.Serum alanine aminotransaminase levels were significantly lower in MMP-9(-/-) mice compared with those in TIMP-1(-/-) mice(WT:476 ± 83 IU/L,MMP-9(-/-):392 ± 30 IU/L,TIMP-1(-/-):673 ± 73 IU/L,P < 0.01).Western blotting and gelatin zymography demonstrated a lack of MMP-9 expression and activity in MMP-9(-/-) mice,which was in contrast to WT and TIMP-1(-/-) mice.No change in MMP-2 expression was observed in any of the study groups.Similar to MMP-9(-/-) mice,when WT mice were treated with MMP-9 monoclonal antibody or the synthetic inhibitor GM6001,hemorrhagic and necrotic lesions were significantly smaller and fewer than in control mice(P < 0.05).These results suggest that MMP-9 plays an important role in the development of parenchymal hemorrhage and necrosis in the small remnant liver.CONCLUSION:Successful MMP-9 inhibition attenuates the formation of hemorrhage and necrosis and might be a potential therapy to ameliorate liver injury after massive hepatectomy. | Norifumi Ohashi Tomohide Hori Florence Chen Sura Jermanus Christopher B Eckman Akimasa Nakao Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，19： | 3 |
| 3 | Biochemical detection of A beta isoforms: implications for pathogenesis, diagnosis, and treatment of Alzheimers disease 显示文摘 | Golde TE Eckman CB Younkin SG | 2000 | Biochim Biophys Acta2000，1502，1： | 2 |
| 4 | Degradation of the Alzheimer's amyloid beta peptide by endothelinconverting enzyme显示文摘 | Reed DK Eckman CB | 2001 | J Biol Chem2001，276，24： | 1 |
| 5 | Correlative memory deficits,αβelevation,and amyloid plaques in transgenic mice显示文摘 | Hsiao K Chapman P Eckman C | 1996 | Science1996，274，5284： | 1 |
| 6 | Markedness and the Contrastive Analysis Hypothesis 显示文摘 | Eckman F | 1977 | Language Learning1977，，27： | 1 |
| 7 | Hydrodynamic processes affecting benthic recruitment显示文摘 | ECKMAN J R | 1983 | Limnology and Oceanography1983，28，2： | 1 |
| 8 | Increased Abeta42(43) from cell lines expressing presenilin 1 mutations显示文摘 | Mehta ND Refolo LM Eckman C | | 0，，： | 1 |
| 9 | Increased amyloid-beta42 ( 43 ) in brains of mice expressing mutant presenilin 1 显示文摘 | Duff K Eckman C Zehr C | 1996 | Nature1996，383，6602： | 1 |
| 10 | Local and long-distance anaphora in second language of English显示文摘 | Eckman F | 1994 | Language learning1994，，40： | 1 |
| 11 | On the generalizationof relative clause instruction in the acquisition of Englishas a second language显示文摘 | Eckman F R Bell L Nelson D | 1988 | Applied Linguistics1988，，9： | 1 |
| 12 | Effects of biological activity on the entrainment of marine sediments 显示文摘 | Nowell A R M Jumars P A Eckman J E | 1981 | Marine Geology1981，42，： | 1 |
| 13 | Membrane depolarization,elevated Ca^2+ entry,and gene expression in cerebral arteries of hypertensive rats显示文摘 | Wellman GC Cartin L Eckman DM | 2001 | Am J Physiol2001，281，： | 1 |
| 14 | The effect of warfarin and intensity of anticoagulation on outcome of intracerebral hemorrhage显示文摘 | Eckman MH Knudsen KA | 2004 | Arch Intern Med2004，164，8： | 1 |
| 15 | The effect of warfarin and intensity of anticoagulation on outcome of intracerebral hemorrhage 显示文摘 | Rosand J Eckman MH Knudsen KA | 2004 | Arch Intern Med2004，164，： | 1 |
| 16 | Markedness and the contrastive analysis hypothesis显示文摘 | Eckman F | 1977 | Language Learning1977，，27： | 1 |
| 17 | A critical evaluation of the priciples governing the advantage of intra-arterial infusion显示文摘 | Eckman W W Pallak C S Fenstenn ach J D | 1974 | J Pharm Acokine Biopharm Aceut1974，2，： | 1 |
| 18 | Markedness and contrastive analysis hypothesis显示文摘 | Eckman F | | 0，，27： | 1 |
| 19 | Markedness and the Contrastive Analysis Hypothesis显示文摘 | | 1977 | Language Learning1977，，： | 1 |
| 20 | Markedness and the contrastive analysis hypothesis显示文摘 | Eckman F | 1977 | Language Learning 271977，，： | 1 |
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