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| 1 | Tissue inhibitor of metalloproteinases-3 is the major metalloproteinase inhibitor in the decidualizing murine uterus显示文摘 | Leco KJ Edwards DR Schultz GA | 1996 | Mol Reprod Dev1996，45，4： | 1 |
| 2 | Assessing the impact of race, social factors and air pollution on birth outcomes: a population-based study显示文摘 | Gray SC Edwards SE Schultz BD | 2014 | Environ Health2014，13，1： | 1 |
| 3 | State feedback assisted classical control: An incremental approach to control modernization of existing and future nuclear reactors and power plants显示文摘 | Edwards R M Lee K Y Schultz M A | 1990 | Nuclear Technology1990，92，2： | 1 |
| 4 | Tissue inhibitor of metalloproteinase 3 is the major metalloproteinase inhibitor in the decidualizing murine uterus显示文摘 | Leco KJ Edwards DR Schultz GA | 1996 | Mol Reprod Dev1996，45，4： | 1 |
| 5 | Assessing the impact of race, social factors and air pollution on birth outcomes: a population-based study 显示文摘 | Gray SC Edwards SE Schultz BD | 2014 | Environ Health2014，13，1： | 1 |
| 6 | An orally active calcium-sensing receptor antagonist that transiently increases plasma concentrations of PTH and stimulates bone formation显示文摘 | Sanjay Kumar Christopher J. Matheny Sandra J. Hoffman Robert W. Marquis Maggie Schultz Xiaoguang Liang Janice A. Vasko George B. Stroup Vernal R. Vaden Hyking Haley John Fox Eric G. DelMar Edward F. Nemeth Amparo M. Lago James F. Callahan Pradip Bhatnagar | 2009 | Bone2009，，2： | 1 |
| 7 | An integrated semiconductor device enabling non-optical genome sequencing显示文摘 | Rothberg JM Hinz W Rearick TM Schultz J Mileski W Davey M Leamon JH Johnson K Milgrew MJ Edwards M Hoon J Simons JF Marran D Myers JW Davidson JF Branting A Nobile JR Puc BP Light D Clark TA Huber M Branciforte JT Stoner IB Cawley SE Lyons M Fu Y Homer N | | 0，，： | 1 |
| 8 | Satellite cell behavior during skeletal muscle growth and regeneration显示文摘 | Bower JJ Edward Schultz | 2003 | Med Sci Sports Exerc2003，13，5： | 1 |
| 9 | Development and mapping of SSR markers for maize显示文摘 | Natalya Sharopova Michael D. McMullen Linda Schultz Steve Schroeder Hector Sanchez-Villeda Jack Gardiner Dean Bergstrom Katherine Houchins Susan Melia-Hancock Theresa Musket Ngozi Duru Mary Polacco Keith Edwards Thomas Ruff James C. Register Cory Brouwer | 2002 | Plant Molecular Biology (-)2002，，5： | 1 |
| 10 | State feedback assisted classical control: An in- cremental approach to control modernization of existing and future nuclear reactors and power plants显示文摘 | EDWARDS R M LEE K Y SCHULTZ M A | 1990 | Nuclear Technology1990，92，2： | 1 |
| 11 | Development and mapping of SSR markers for maize显示文摘 | Natalya Sharopova Michael D. McMullen Linda Schultz Steve Schroeder Hector Sanchez-Villeda Jack Gardiner Dean Bergstrom Katherine Houchins Susan Melia-Hancock Theresa Musket Ngozi Duru Mary Polacco Keith Edwards Thomas Ruff James C. Register Cory Brouwer | 2002 | Plant Molecular Biology (-)2002，，5： | 1 |
| 12 | Connective tissue growth factor differentially binds to members of the cystine knot superfamily and potentiates platelet-derived growth factor-B signaling in rabbit corneal fibroblast cells显示文摘AIM:To study the binding of connective tissue growth factor(CTGF) to cystine knot-containing ligands and how this impacts platelet-derived growth factor(PDGF)-B signaling. METHODS:The binding strengths of CTGF to cystine knot-containing growth factors including vascular en-dothelial growth factor(VEGF)-A,PDGF-B,bone morphogenetic protein(BMP)-4,and transforming growth factor(TGF)-β1 were compared using the LexA-based yeast two-hybrid system. EYG48 reporter strain that carried a wild-type LEU2 gene under the control of Lex A operators and a lac Z reporter plasmid(p80plac Z) containing eight high affinity Lex A binding sites were used in the yeast two-hybrid analysis. Interactions between CTGF and the tested growth factors were evaluated based on growth of transformed yeast cells on selective media and colorimetric detection in a liquid β-galactosidase activity assay. Dissociation constants of CTGF to VEGF-A isoform 165 or PDGF-BB homo-dimer were measured in surface plasma resonance(SPR) analysis. CTGF regulation in PDGF-B presentation to the PDGF receptor β(PDGFRβ) was also quantitatively assessed by the SPR analysis. Combinational effects of CTGF protein and PDGF-BB on activation of PDGFRβ and downstream signaling molecules ERK1/2 and AKT were assessed in rabbit corneal fibroblast cells by Western analysis. RESULTS:In the LexA-based yeast two-hybrid system,cystine knot motifs of tested growth factors were fused to the activation domain of the transcriptional factor GAL4 while CTGF was fused to the DNA binding domain of the bacterial repressor protein Lex A. Yeast cotransformants containing corresponding fusion proteins for CTGF and all four tested cystine knot motifs survived on selective medium containing galactose and raffinose but lacking histidine,tryptophan,and uracil. In liquid β-galactosidase assays,CTGF expressing cells that were co-transformed with the cystine knot of VEGF-A had the highest activity,at 29.88 ± 0.91 fold above controls(P < 0.01). Cells containing the cystine knot of BMP-4 expressed the second most activity,with a 24.77 ± 0.47 fold increase(P < 0.01). Cells that contained the cystine knot of TGF-β1 had a 3.80 ± 0.66 fold increase(P < 0.05) and the ones with the cystine knot of PDGF-B had a 2.64 ± 0.33 fold increase of β-galactosidase activity(P < 0.01). Further SPR analysis showed that the association rate between VEGF-A 165 and CTGF was faster than PDGF-BB and CTGF. The calculated dissociation constant(KD) of CTGF to VEGF165 and PDGF-BB was 1.8 and 43 nmol/L respectively. PDGFBB ligand and PDGFRβ receptor formed a stable complex with a low dissociation constant 1.4 nmol/L. Increasing the concentration of CTGF up to 263.2 nmol/L significantly the ligand/receptor binding. In addition,CTGF potentiated phosphorylation of PDGFRβ and AKT in rabbit corneal fibroblast cells stimulated by PDGFBB in tissue culture condition. In contrast,CTGF did not affect PDGF-B induced phosphorylation of ERK1/2.CONCLUSION:CTGF has a differential binding affinity to VEGF-A,PDGF-B,BMP-4,and TGF-β. Its weak association with PDGF-B may represent a novel mechanism to enhance PDGF-B signaling. | Liya Pi Pei-Yu Chung Sriniwas Sriram Masmudur M Rahman Wen-Yuan Song Edward W Scott Bryon E Petersen Gregory S Schultz | 2015 | World Journal of Biological Chemistry2015，6，4： | 0 |
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