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| 1 | 砷对NIH 3T3细胞谷胱甘肽水平、相关酶活性及其基因表达的影响显示文摘[目的]探讨急性、慢性砷暴露对小鼠成纤维细胞 (NIH3T3细胞 )氧化还原状态的影响 ,进而探讨其对细胞谷胱甘肽 (GSH)水平、相关酶活性及其基因表达的影响。[方法]将经过不同浓度砷处理的细胞 ,抽提细胞总蛋白。采用经过改进的Clark法测定细胞中谷胱甘肽 ;采用Thomas法测定谷胱甘肽还原酶 (GR)活性 ;使用分光光度法测定谷胱甘肽与1 氯 2 ,4 二硝基苯 (CDNB)的结合以确定谷胱甘肽转移酶 (GST)活性。采用SDS聚丙烯酰胺凝胶电泳和固定化蛋白质的免疫学测定法测定血红素氧化酶 (HO 1)蛋白表达 ;采用RNA印迹法测定谷胱甘肽还原酶 (GR)和谷胱甘肽过氧化物酶(GSH Px)的基因表达。[结果]急性砷处理细胞在较低浓度范围可以引起GSH水平的增加 ;而当砷浓度高于25μmol/L时 ,GSH水平降低 ;在0.1和0.5 μmol/L慢性染毒之后细胞GSH水平明显降低。然而 ,在急性和慢性砷处理组GST和GR的活性均有升高 ,而在高浓度24h处理组则显著降低 ;GR的mRNA水平与GR的活性相一致 ,在急、慢性染毒组中均表现为GR的mRNA表达上调 ;砷可以引起血红素氧化酶 1(HO 1)蛋白水平的增加。急性、慢性砷处理均引起GSH Px mRNA表达下调。[结论]三价砷可以引起氧化应激、GSH水平改变、以及相关酶活性及其基因表达的改变 ,急性与慢性砷接触对GSH系统? | 胡宇 金喜梅 王国荃 Snow Elizabeth T | 2003 | 环境与职业医学2003，20，6： | 8 |
| 2 | Impact of enclosure management on soil properties and microbial biomass in a restored semi-arid rangeland, Kenya显示文摘Rangeland degradation is a serious problem throughout sub-Saharan Africa and its restoration is a challenge for the management of arid and semi-arid areas. In Lake Baringo Basin of Kenya, communities and individual farmers are restoring indigenous vegetation inside enclosures in an effort to combat severe land degradation and address their livelihood problems. This study evaluated the impact of enclosure management on soil properties and microbial biomass, being key indicators of soil ecosystem health. Six reseeded communal enclosures using soil embankments as water-harvesting structures and strictly regulated access were selected, varying in age from 13 to 23 years. In six private enclosures, ranging from 3 to 17 years in age, individual farmers emulated the communal enclosure strategy and restored areas for their exclusive use. Significant decreases in bulk density, and increases in the soil organic carbon, total nitrogen and microbial biomass contents and stocks were found in the enclosures as compared with the degraded open rangeland. In the private enclosures, the impact of rehabilitation on the soil quality was variable, and soil quality was in general lower than that obtained under communal management. The significant increase of absolute stocks of carbon, nitrogen and microbial biomass compared to the degraded open rangeland indicates the potential for the restoration of soil quality through range rehabilitation. Over-sowing with indigenous legume fodder species could improve total nitrogen content in the soil and nutritional value of the pastures as well. | Stephen M MUREITHI Ann VERDOODT Charles KK GACHENE Jesse T NJOKA Vivian O WASONGA Stefaan De NEVE Elizabeth MEYERHOFF Eric Van RANST | 2014 | Journal of Arid Land2014，6，5： | 3 |
| 3 | Positron emission tomography scanning in the evaluation of hepatocellular carcinoma显示文摘 | M.Akram Khan Connie S Combs Elizabeth M Brunt Val J Lowe Michael K Wolverson Harvey Solomon Brian T Collins Adrian M.Di Bisceglie | 2000 | Journal of Hepatology2000，，5： | 2 |
| 4 | Firefly lueiferin-aetivated rose bengal显示文摘 | Theodossiou T Hothersall J S Elizabeth A | 2003 | Cancer Re- search2003，63，： | 1 |
| 5 | Serotonin receptors expressed by myelinating Schwann cells in rat sciatic nerve 显示文摘 | Elizabeth JY Hadassah T Mark HE | 1997 | Brain Research1997，753，2： | 1 |
| 6 | Some immunological relationshios of ct-lactalbumin and 13-1actoglabulin in miluks of various species显示文摘 | JOHKE T ELIZABETH C HAGEMAN L | 1962 | Biol Chem1962，2，7： | 1 |
| 7 | Effects of Chromium on DNA Replication In Vitro显示文摘 | Elizabeth T S | 1994 | Environmental Health Perspectives1994，102，3： | 1 |
| 8 | Impact of nutrition on PCB toxicity显示文摘 | Zuzana M Elizabeth O Michal T Bernhard H | 2007 | Environmental Toxicology and Pharmacology2007，25，2： | 1 |
| 9 | Information Need Use Studies显示文摘 | Hewins Elizabeth T | 1990 | ARIST1990，，25： | 1 |
| 10 | Cognitive dysfunc- tion and diabetes mellitus 显示文摘 | Christopher T Kodl R Elizabeth R | 2008 | Endocr Rev2008，29，4： | 1 |
| 11 | Providing support for problem-based learning in dentistry: the Manchester experience显示文摘 | Gillian HR Elizabeth T | 2003 | Eur J Dent Educ2003，7，： | 1 |
| 12 | Information Need Use Studies显示文摘 | Hewins?Elizabeth T | 1990 | ARIST1990，，： | 1 |
| 13 | High- fat/fructose feeding during prenatal and postnatal devel- opment in female rats increases susceptibility to renal and metabolic injury later in life-American journal of physiol- ogy显示文摘 | Elizabeth R Flynn-Barbara T Alexander J | 2013 | Regulatory Integrative and Comparative Physiol- ogy2013，304，4： | 1 |
| 14 | Cepharanthine is a potent reversal agent for MRP7 (ABCC10)-mediated multidrug resistance显示文摘 | Zhou Y Elizabeth H B Shen T | 2009 | Biochemical Pharmacology2009，77，6： | 1 |
| 15 | Effect of arsenic on transcription factor AP-1 and NF-KB DNA binding activity and related gene expression显示文摘 | Hu Yu Jin Ximei Snow Elizabeth T | 2002 | Toxicology Letters2002，133，1： | 1 |
| 16 | Insulin-like growth factor binding protein-5 (IGFBP-5) induces premature cell death in the mammary glands of transgenic mice 显示文摘 | ELIZABETH T MICHAEL C B GORDON J A | 2002 | Development2002，129，19： | 1 |
| 17 | Distribution, speciation, and transport of mercury in stream-sediment, stream-water, and fish collected near abandoned mercury mines in southwestern Alaska, USA显示文摘 | John E G Peter M T Elizabeth A B | 2000 | Science of The Total Environment2000，260，： | 1 |
| 18 | Heavy metal removal in anaerobic semi-continuous stirred tank reactors by a consortiumof sulfate-reducing bacteria显示文摘 | Hoa T Q Kieu Elizabeth Müller Harald Horn | 2011 | Water Research2011，45，13： | 1 |
| 19 | Application of micro- bore HPLC in combination with tandem MS for the quantification of rosuvastatin in human plasma显示文摘 | Kathalijne AO Timothy SI Elizabeth T etal | 2006 | Journal of Chromatography B2006，832，： | 1 |
| 20 | Myocardial mitochondrial calcium accumulation modulates nuclear calcium accumulation and DNA, Fragmentation 显示文摘 | Elizabeth AF James DM Takttro T | 1995 | Amm Thorac Surg1995，60，： | 1 |
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