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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Taste and mouth-feel properties of different types of tannin-like polyphenolic compounds and anthocyanins in wine显示文摘 | Stéphane Vidal Leigh Francis Ann Noble Mariola Kwiatkowski Véronique Cheynier Elizabeth Waters | 2003 | Analytica Chimica Acta2003，，1： | 2 |
| 3 | Interleukin-1β messenger ribonucleic acid and protein levels after fluid-percussion brain injury in rats: importance of injury severity and brain temperature显示文摘 | Kosaku K Katina C Elizabeth V | 2002 | Neurosurgery2002，51，1： | 1 |
| 4 | Adjacent segment disease : an uncommon complication after cervical expansile laminoplasty : case re- port显示文摘 | Michael Y Barth A Elizabeth V | 2003 | Neurosurgery2003，53，： | 1 |
| 5 | Relevance ranking for one to three term queries显示文摘 | Charles L A Clarke Gordon V Cormack Elizabeth A Tudhope | 2000 | Information Processing and Management2000，，36： | 1 |
| 6 | Reduced Amygdala Response to Fearful Expressions in Children and Adolescents With Callous-Unemotional Traits and Disruptive Behavior Disorders显示文摘 | Marsh Abigail A Finger Elizabeth C Mitchell Derek G V Reid Marguerite E Sims Courtney Kosson David S Towbin Kenneth E Leibenluft Ellen Pine Daniel S Blair R J R | 2008 | The American Journal of Psychiatry2008，，6： | 1 |
| 7 | Contribution to the determination of kinetic parameters of the sol-gel transformation by rheological measurements显示文摘 | Peter J D Brian V Elizabeth W | 2000 | J Colloid Interface Sci2000，221，： | 1 |
| 8 | Pre concentration of benzalkonium chloride using sodium dodecyl sulfate attached to a strong anion exchange resin显示文摘 | KYLE A J ELIZABETH G V ERIC D C | 1999 | Functional Polymers1999，40，： | 1 |
| 9 | Community-acquired bloodstream infections in Africa: a systematic review and meta-analysis显示文摘 | Elizabeth A Reddy Andrea V Shaw John A Crump | 2010 | The Lancet Infectious Diseases2010，，6： | 1 |
| 10 | Salt-marsh processes : a review显示文摘 | LIU X B ELIZABETH J J PETER V F J | 1993 | Environmental Toxicology and Chemistry1993，12，12： | 1 |
| 11 | Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea(Aspal- athus linearis ) α-Tocopherol, BHT and BHA 显示文摘 | ASTRID V G ELIZABETH J CHRIS F H | 1997 | J Agrie Food Chem1997，45，： | 1 |
| 12 | Effect of heat treatments on Canola Press Oils显示文摘 | Elizabeth M P Vivekenand S V Frank W S | 1991 | Journal of the American Oil Chemists'' Society1991，68，6： | 1 |
| 13 | Opportunities and Challenges in Using Epidemiologic Methods to Monitor Drug Safety in the Era of Large Automated Health Databases显示文摘 | Elizabeth B Andrews Andrea V Margulis Patricia Tennis | 2014 | Curr Epidemiol Rep2014，1，： | 1 |
| 14 | Effects of Fusion Zone Size and Failure Mode on Peak Load and Energy Absorption of Advanced High Strength Steel Spot Welds Under Lap Shear Loading Conditions 显示文摘 | Xin Sun Elizabeth V Stephens Mohammad A Khaleel | 2008 | Engineering Failure Analysis2008，15，： | 1 |
| 15 | Living donor livertransplantation in adults : vascular variants important in surgicalplanning for donors and recipients 显示文摘 | Erbay N Raptopoulos V Elizabeth A | 2003 | AJR2003，，181： | 1 |
| 16 | MEK Kinase 1 induces mitochondrial permeability transition leading to apoptosis independent of cytochrome C release显示文摘 | Erika MG Elizabeth SH Jacylyn V | 2002 | J Biol Chem2002，277，10： | 1 |
| 17 | Fatigue behaviors of self-piercing rivets joining similar and dissimilar sheet metals显示文摘 | Sun Xin Stephens Elizabeth V Khaleel Moe A | | 0，，20： | 1 |
| 18 | Quantitative StructureActivity Relationship in Aziridinyl-1,4-naphthoquinone Antimalarials:Study of Theoretical Correlations by the PM3 Method显示文摘 | ELIZABETH V M JOSE W VITOR F F | 2004 | Bioorganic and Medicinal Chemistry2004，12，： | 1 |
| 19 | Inhibition of complement activation by nat- ural sulfated polysaccharide (Fucans) from brown seaweed显示文摘 | CATHERINE B ELIZABETH F CATHERINE B V | 1994 | Molecular Immunology1994，31，4： | 1 |
| 20 | American societyof plant physiologists heat shock protein 101 plays a crucial rolein thermotolerance in arabidopsis显示文摘 | Christine Q Suk-Whan H Elizabeth V | 2000 | The Plant Cell2000，12，4： | 1 |
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