| 1 | Comparative transcriptome analysis of purple-fleshed sweet potato provides insights into the molecular mechanism of anthocyanin biosynthesis显示文摘Sweet potato, Ipomoea batatas, is a globally important food crop. The purple-fleshed sweet potato, rich in anthocyanins, has great potential for both nutritional and pharmaceutical uses. In this study, we characterized the root transcriptomes of the purple-fleshed sweet potato cv.Jingshu 6 and its mutant JS6-5 with high anthocyanin content by high-throughput RNA sequencing. A total of22873364 and 27955097 high quality reads were obtained from Jingshu 6 and JS6-5, respectively, and assembled into35592 unigenes. In all, we obtained 1566 differentially expressed genes(DEGs). Among them, 994 were upregulated and 572 were downregulated in JS6-5 compared to the expression in Jingshu 6. A total of 1436 DEGs were annotated, in which 847 DEGs had gene ontology(GO) terms and 329 DEGs were assigned to 84 Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways. Most importantly, 23 differentially expressed genes and 24 transcription factors were identified as candidate genes involved in anthocyanin biosynthesis. In addition, 2349 SSRs were detected. This study not only provides the candidate genes but also provides insights into the molecular mechanism of anthocyanin biosynthesis in sweet potato. | Hongyuan ZHAO Shanshan ZHANG Feibing WANG Ning ZHAO Shaozhen HE Qingchang LIU Hong ZHAI | 2018 | Frontiers of Agricultural Science and Engineering2018，5，2： | 2 |
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