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| 1 | SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza显示文摘MYB transcription factors play vital roles in plant growth and metabolism.The phytohormone methyl jasmonate(MeJA)promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza,but the regulatory mechanism is poorly understood.Here,we identified the MeJA-responsive R2R3-MYB transcription factor gene 5mMYB2 from a transcriptome library produced from MeJAtreated S.mi'/horr/ifza hairy roots.Sm/VIYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP^8Ai^.5mMYB2 was highly expressed in the periderm of S.miltiorrhiza and SmMYB2 localized to the nucleus.Overexpressing Sml\AYB2 in S.miltiorrhiza hairy roots significantly increased the levels of salvianolic acids(including rosmarinic acid and salvianolic acid B)by upregulating salvianolic acid biosynthetic genes such as CYP98A14.SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14,as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays.Anthocyanin contents were significantly higher in Sm/ViyB2-overexpressing hairy root lines than the control,primarily due to the increased expression of CHI,DFR,and ANS.These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis,providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network. | Changping Deng Yao Wang Fenfen Huang Sunjie Lu Limei Zhao Xingyuan Ma Guoyin Kai | 2020 | Journal of Integrative Plant Biology2020，62，11： | 6 |
| 2 | Association between 15 known or potential breast cancer susceptibility genes and breast cancer risks in Chinese women显示文摘Objective:There are many hereditary breast cancer patients in China,and multigene panel testing has been a new paradigm of genetic testing for these patients and their relatives.However,the magnitude of breast cancer risks related to multiple breast cancer susceptibility genes are largely unknown in Chinese women.Methods:We screened pathogenic variants in 15 established or potential breast cancer susceptibility genes from 8,067 consecutive Chinese female breast cancer patients and 13,129 Chinese cancer-free female controls.These breast cancer patients were unselected for age at diagnosis or family history.Results:We found that pathogenic variants in TP53[odds ratio(OR):16.9,95%confidence interval(CI):5.2–55.2];BRCA2(OR:10.4,95%CI:7.6–14.2);BRCA1(OR:9.7,95%CI:6.3–14.8);and PALB2(OR:5.2,95%CI:3.0–8.8)were associated with a high risk of breast cancer.ATM,BARD1,CHEK2,and RAD51D were associated with a moderate risk of breast cancer with ORs ranging from 2-fold to 4-fold.In contrast,pathogenic variants of NBN,RAD50,BRIP1,and RAD51C were not associated with increased risk of breast cancer in Chinese women.The pathogenic variants of PTEN,CDH1,and STK11 were very rare,so they had a limited contribution to Chinese breast cancer.Patients with pathogenic variants of TP53,BRCA1,BRCA2,and PALB2 more often had earlyonset breast cancer,bilateral breast cancer,and a family history of breast cancer and/or any cancer.Conclusions:This study provided breast cancer risk assessment data for multiple genes in Chinese women,which is useful for genetic testing and clinical management of Chinese hereditary breast cancer. | Fenfen Fu Dongjie Zhang Li Hu Senthil Sundaram Dingge Ying Ying Zhang Shuna Fu Juan Zhang Lu Yao Ye Xu Yuntao Xie | 2022 | Cancer Biology & Medicine2022，19，2： | 3 |
| 3 | The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza显示文摘Water-soluble phenolic acids are major bioactive compounds in the medicinal plant species Salvia miltiorrhiza.Phenolic acid biosynthesis is induced by methyl jasmonate(MeJA)in this important Chinese herb.Here,we investigated the mechanism underlying this induction by analyzing a transcriptome library of S.miltiorrhiza in response to MeJA.Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1.Overexpressing SmMYB1 significantly promoted phenolic acid accumulation and upregulated the expression of genes encoding key enzymes in the phenolic acid biosynthesis pathway,including cytochrome P450-dependent monooxygenase(CYP98A14).Dual-luciferase(dual-LUC)assays and/or an electrophoretic mobility shift assays(EMSAs)indicated that SmMYB1 activated the expression of CYP98A14,as well as the expression of genes encoding anthocyanin biosynthesis pathway enzymes,including chalcone isomerase(CHI)and anthocyanidin synthase(ANS).In addition,SmMYB1 was shown to interact with SmMYC2 to additively promote CYP98A14 expression compared to the action of SmMYB1 alone.Taken together,these results demonstrate that SmMYB1 is an activator that improves the accumulation of phenolic acids and anthocyanins in S.miltiorrhiza.These findings lay the foundation for in-depth studies of the molecular mechanism underlying MeJA-mediated phenolic acid biosynthesis and for the metabolic engineering of bioactive ingredients in S.miltiorrhiza. | Wei Zhou Min Shi Changping Deng Sunjie Lu Fenfen Huang Yao Wang Guoyin Kai | 2021 | Horticulture Research2021，8，1： | 3 |
| 4 | Plant-Me- diated synthesis of Ag-Pd alloy nanoparticles and their applica- tion as catalyst toward selective hydrogenation显示文摘 | FENFEN LU DAOHUA SUN JIALE HUANG | 2014 | ACS Sustain- able Chemistry Engineering2014，2，： | 1 |
| 5 | miR-375 Is Down-Regulated in Squamous Cervical Cancer and Inhibits Cell Migration and Invasion via Targeting Transcription Factor SP1显示文摘 | Fenfen Wang Yang Li Jiansong Zhou Junfen Xu Chanjuan Peng Feng Ye Yuanming Shen Weiguo Lu Xiaoyun Wan Xing Xie | 2011 | The American Journal of Pathology2011，，5： | 1 |
| 6 | miR-375 Is Down-Regulated in Squamous Cervical Cancer and Inhibits Cell Migration and Invasion via Targeting Transcription Factor SP1显示文摘 | Fenfen Wang Yang Li Jiansong Zhou Junfen Xu Chanjuan Peng Feng Ye Yuanming Shen Weiguo Lu Xiaoyun Wan Xing Xie | 2011 | The American Journal of Pathology2011，，5： | 1 |
| 7 | Identification of suitable reference genes for measurement of gene expression in human cervical tissues显示文摘 | Yuanming Shen Yang Li Feng Ye Fenfen Wang Weiguo Lu Xing Xie | 2010 | Analytical Biochemistry2010，，2： | 1 |
| 8 | Biosynthesized Gold/Activated Carbon Catalyst for Aerobic Glucose Oxidation: Influence of Acid Treatment on Activated Carbon显示文摘 | Fenfen Lu Yan Xu Xia Jiang Daohua Sun Qingbiao Li | 2017 | Chinese Journal of Chemistry2017，35，5： | 1 |
| 9 | Progress in quantitative research on the relationship between atmospheric oxidation and air quality显示文摘Atmospheric oxidizing capacity(AOC)is an essential driving force of troposphere chemistry and self-cleaning,but the definition of AOC and its quantitative representation remain uncertain.Driven by national demand for air pollution control in recent years,Chinese scholars have carried out studies on theories of atmospheric chemistry and have made considerable progress in AOC research.This paper will give a brief review of these developments.First,AOC indexes were established that represent apparent atmospheric oxidizing ability(AOIe)and potential atmospheric oxidizing ability(AOIp)based on aspects of macrothermodynamics and microdynamics,respectively.A closed study refined the quantitative contributions of heterogeneous chemistry to AOC in Beijing,and these AOC methods were further applied in Beijing-Tianjin-Hebei and key areas across the country.In addition,the detection of ground or vertical profiles for atmospheric OH·,HO_(2)·,NO_(3)·radicals and reservoir molecules can now be obtained with domestic instruments in diverse environments.Moreover,laboratory smoke chamber simulations revealed heterogeneous processes involving reactions of O_(3)and NO_(2),which are typical oxidants in the surface/interface atmosphere,and the evolutionary and budgetary implications of atmospheric oxidants reacting under multispecies,multiphase and multi-interface conditions were obtained.Finally,based on the GRAPES-CUACE adjoint model improved by Chinese scholars,simulations of key substances affecting atmospheric oxidation and secondary organic and inorganic aerosol formation have been optimized.Normalized numerical simulations of AOIe and AOIp were performed,and regional coordination of AOC was adjusted.An optimized plan for controlling O_(3)and PM2.5was analyzed by scenario simulation. | Yuesi Wang Xin Jin Zirui Liu Gehui Wang Guiqian Tang Keding Lu Bo Hu Shanshan Wang Guohui Li Xinqin An Chao Wang Qihou Hu Lingyan He Fenfen Zhang Yuanhang Zhang | 2023 | Journal of Environmental Sciences2023，，1： | 1 |
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