|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis显示文摘MSH5,傻瓜相当或相同的事物脱氧核糖核酸失配修理蛋白质家庭的一个成员,被显示了在象老鼠,开始发育的酵母和 Caenorhabditis elegans 那样的多样的有机体为合适的同源染色体再结合被要求。在这篇论文,我们证明带通常认为的破坏 AtMSH5 基因的变异的 Arabidopsis 植物在 meiotic 期间展出缺点,生产不能生存的花粉粒和反常胚囊的一个比例,并且从而导致在富饶的减少。AtMSH5 表示被限制到 meiotic 花发芽,它在成熟分裂期间与一个可能的角色一致。男成熟分裂的 Cytological 分析从 diplotene 揭示了众多的 univalents 的存在到中期我,它在交叉频率与大减小被联系。在异种的剩余 chiasmata 的平均数字每性母细胞被归结为 2.54,它在野生型占约 25% 数量。这里,量的细胞遗传学的分析表明在 Atmsh5 异种的剩余 chiasmata 在 meiocytes 之中是随机分布式的,建议 AtMSH5 有在干扰敏感的交叉形成期间的一个必要角色。总起来说,证据显示 AtMSH5 通过在前期期间便于交叉形成支持相应再结合我在 Arabidopsis。 | Xiaoduo Lu Xiaolin Liu Lizhe An Wei Zhang Jian Sun Huijuan Pei Hongyan Meng Yunliu Fan Chunyi Zhang | 2008 | Cell Research2008，18，5： | 6 |
| 2 | SEC1A and SEC6 synergistically regulate pollen tube polar growth显示文摘Pollen tube polar growth is a key physiological activity for angiosperms to complete double fertilization, which is highly dependent on the transport of polar substances mediated by secretory vesicles.The exocyst and Sec1/Munc18(SM) proteins are involved in the regulation of the tethering and fusion of vesicles and plasma membranes, but the molecular mechanism by which they regulate pollen tube polar growth is still unclear. In this study, we found that loss of function of SEC1A, a member of the SM protein family in Arabidopsis thaliana, resulted in reducing pollen tube growth and a significant increase in pollen tube width. SEC1A was diffusely distributed in the pollen tube cytoplasm, and was more concentrated at the tip of the pollen tube. Through coimmunoprecipitation-mass spectrometry screening,protein interaction analysis and in vivo microscopy,we found that SEC1A interacted with the exocyst subunit SEC6, and they mutually affected the distribution and secretion rate at the tip of the pollen tube. Meanwhile, the functional loss of SEC1A and SEC6 significantly affected the distribution of the SNARE(soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex member SYP125 at the tip of the pollen tube, and led to the disorder of pollen tube cell wall components. Genetic analysis revealed that the pollen tube-related phenotype of the sec1a sec6 double mutant was significantly enhanced compared with their respective single mutants. Therefore, we speculated that SEC1A and SEC6 cooperatively regulate the fusion of secretory vesicles and plasma membranes in pollen tubes, thereby affecting the length and the width of pollen tubes. | Tingting Fan Yuemin Fan Yang Yang Dong Qian Yue Niu Lizhe An Yun Xiang | 2023 | Journal of Integrative Plant Biology2023，65，7： | 1 |
| 3 | Formulation of Classical Swine Fever Immunization Procedures in Large scale Pig Farms显示文摘[Objective] The paper was to formulate classical swine fever( CSF) immunization procedures in large-scale pig farms. [Method] Antibody maintenance time and dynamics of material antibody was detected by enzyme-linked immunosorbent assay( ELISA),and scientific primary immunization time of piglets was explored. [Result] The positive rate of sows reached the highest level of 93% at 4 weeks post immunization. Piglets were immunized at 9 weeks of age,the antibody level did not increase at 3 weeks post immunization,but increased rapidly after secondary immunization; the antibody level in late fattening period was high,and pigs could be protected completely. [Conclusion]General prevention of CSF is suitable for scale farming. It takes about 4 weeks to produce CSF antibody. Therefore,pig farms should conduct immunization timely at one month prior to susceptible stage according to their infection pressure. | He Junchang Zhang Jianyuan Xie Youfu Fan Lizhe Gao Shihua | 2017 | Animal Husbandry and Feed Science2017，9，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员