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| 1 | Extraction, identification, and antioxidant property evaluation of limonin from pummelo seeds显示文摘Limonin, the main bioactive phytochemical constituent of limonoids with multi-functions, is enriched in citrus fruits and often found at a high concentration in citrus seeds. The present study was attempted to introduce a new and efficient extraction method to isolate limonoids from pummelo seeds, and to evaluate the antioxidant property of the main constituent limonin in Hep G2 cells. Three key single factors were identified for the extraction of limonoids from pummelo seeds using the Box-Behnken experiment design of response surface methodology(RSM), and the optimized extraction parameters were treatment with 89.68 m L of anhydrous acetone for 4.62 h at 78.94C, while the yield of limonoids was 11.52 mg/g. The structure of isolated main constituent of the limonoids was further identified as limonin by Fourier transform infrared(FT-IR) spectrometer and nuclear magnetic resonance(NMR)spectrum. Moreover, the molecular data in Hep G2 cells revealed that limonin exerted its anti-oxidant property mainly by the activation of nuclear factor(erythroid-2)-like 2(Nrf2)/kelch-like ECHassociated protein 1(Keap1)-antioxidant response element(ARE) pathway in the form of transcriptional regulation of Nrf2 m RNA and posttranscriptional regulation of Nrf2/Keap1 system. These results demonstrate that pummelo seeds are an ideal source of limonoids, and limonin is proved to exert its anti-oxidant property by the activation of Nrf2/Keap1 pathway. | Si Qin Chenghao Lv Qingshan Wang Zhibing Zheng Xi Sun Minyi Tang Fangming Deng | 2018 | Animal Nutrition2018，，3： | 10 |
| 2 | Lotus seed skin proanthocyanidin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway显示文摘Lotus seed is well known as traditional food and medicine,but its skin is usually discarded.Recent studies have shown that lotus seed skin contains a high concentration of proanthocyanidins that have multi-functions,such as antioxidation,anti-inflammation,and anti-cancer effects.In the present study,we aimed to isolate and purify the proanthocyanidins from lotus seed skin by acetone extraction and rotary evaporation,identify their chemical structures by HPLC-MS-MS and NMR,and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in vivo.The results showed that PMR mainly contained oligomeric proanthocyanidins,especially dimeric procyanidin B1 (PB1),procyanidin B2 and procyanidin B4.Although it had limited ability to directly scavenge radicals in vitro,PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factory-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 cells.Molecular data revealed that PB1,a major component in PMR,stabilized Nrf2 by inhibiting the ubiquitination of Nrf2,which led to subsequent activation of the Nrf2-ARE pathway,including the enhancements of Nrf2 nuclear translocation,Nrf2-ARE binding and ARE transcriptional activity.Moreover,the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of PMR.These results revealed that lotus seed skin is a promising resource for functional food development. | Tao Li Qili Li Weiguo Wu Yong Li De-xing Hou Hua Xu Baodong Zheng Shaoxiao Zeng Yang Shan Xiangyang Lu Fangming Deng Si Qin | 2019 | Acta Biochimica et Biophysica Sinica2019，51，1： | 8 |
| 3 | Deficient Rnf43 potentiates hyperactive Kras-mediated pancreatic preneoplasia initiation and malignant transformation显示文摘Background:Largely due to incidental detection,asymptomatic pancreatic cystic le-sions(PCLs)have become prevalent in recent years.Among them,intraductal papillary mucinous neoplasm(IPMN)infrequently advances to pancreatic ductal adenocarcinoma(PDAC).Conservative surveillance versus surgical intervention is a difficult clinical decision for both caregivers and PCL patients.Because RNF43 loss-of-function mutations and KRAS gain-of-function mutations concur in a subset of IPMN and PDAC,their biological significance and therapeutic potential should be elucidated.Methods:Pancreatic Rnf43 knockout and Kras activated mice(Rnf43^(−/−);Kras^(G12D))were generated to evaluate their clinical significance in pancreatic pre-neoplastic initiation and malignant transformation.Results:Loss of Rnf43 potentiated the occurrence and severity of IPMN and PDAC in oncogenic Kras mice.The Wnt/β-catenin signaling pathway was activated in pan-creatic Kras^(G12D)and Rnf43 knockout mice and the PORCN inhibitor LGK974 blocked pancreatic IPMN initiation and progression to PDAC accordingly.Conclusions:Rnf43 is a tumor suppressor in the prevention of pancreatic malignant transformation.This genetically reconstituted autochthonous pancreatic Rnf43^(−/−);Kras^(G12D)preclinical cancer model recapitulates the pathological process from pancreatic cyst to cancer in humans and can be treated with inhibitors of Wnt/β-catenin signaling.Since the presence of RNF43 and KRAS mutations in IPMNs predicts future development of advanced neoplasia from PCLs,patients with these genetic anomalies warrant surveillance,surgery,and/or targeted therapeutics such as Wnt/β-catenin inhibitors. | Xian Zhou Zhichao Sun Mengdi Zhang Xiaoyu Qu Shuhui Yang Lianmei Wang Yanling Jing Li Li Weiwei Deng Fangming Liu Jin Di Jie Chen Jian Wu Hongbing Zhang | 2022 | Animal Models and Experimental Medicine2022，5，1： | 3 |
| 4 | Delphinidin-induced autophagy protects pancreatic β cells against apoptosis resulting from high-glucose stress via AMPK signaling pathway显示文摘Hyperglycemia,a diagnostic characteristic of diabetes mellitus,is detrimental to pancreatic 0 cells.Delphinidin,a member of the anthocyanin family,inhibits glucose absorption,increases glucagonlike peptide(GLP-1)secretion,and improves insulin secretion in diabetes.However,whether delphinidin plays a protective role in pancreatic p-cell mass and function is not clear.In this study,delphinidin was found to decrease the high-glucose-induced apoptosis of RIN-m5F pancreatic β cells.In addition,delphinidin induced autophagy in RIN-m5F cells under the normal and highglucose conditions,while 3-methyladenine(3-MA)inhibition of autophagy significantly diminished the protective role of delphinidin against high-glucose-induced apoptosis of pancreatic β cells.Delphinidin also decreased the level of cleaved caspase 3 and increased the phosphorylation level of AMP-activated protein kinase a(AMPKa)Thr172.Compound C,an AMPK inhibitor,was found to decrease the ratio of LC3-II/LC3-I,and the apoptotic rate of high-glucose-injured cells was in creased after treatme nt with delphinidin,in dicating that delphinidi n attenuated the n egative effects of high-glucose stress to cells.In con elusion,our data dem on strate that delphi nidin protects pancreatic B cells against high-glucose-induced injury by autophagy regulation via the AMPK signaling pathway.These findings might shed light on the underlying mechanisms of diabetes and help improve the prevention and therapy of this common disease. | Dengni Lai Mingyong Huang Lingyan Zhao Yan Tian Yong Li Dongpo Liu Yanyang Wu Fangming Deng | 2019 | Acta Biochimica et Biophysica Sinica2019，51，12： | 3 |
| 5 | Regulation of jasmonate signaling by reversible acetylation of TOPLESS in Arabidopsis显示文摘The plant hormone jasmonate(JA)regulates plant immunity and adaptive growth by orchestrating a genome-wide transcriptional program.Key regulators of JA-responsive gene expression include the master transcription factor MYc2,which is repressed by the conserved Groucho/Tup1-like corepressor TOPLESS(TPL)in the resting state.However,the mechanisms underlying TPL-mediated transcriptional repression of MYc2 activity and hormone-dependent switching between repression and de-repression remainenigmatic.Here,we report the regulation of TPLactivity and JAsignaling byreversibleacetylation of TPL.We found that the histone acetyltransferase GCN5 could mediate TPL acetylation,which enhances its interaction with the NOVEL-INTERACTOR-OF-JAZ(NINJA)adaptor and promotes its recruitment to MYc2 target promoters,facilitating transcriptional repression.Conversely,TPL deacetylation by the histonedeacetylase HDA6 weakens TPL-NINJA interaction and inhibitsTPL recruitmentto MYC2 target promoters,facilitating transcriptional activation.In the resting state,the opposing activities of GCN5 and HDA6 maintain TPL acetylation homeostasis,promoting transcriptional repression activity of TPL.In response to JA elicitation,HDA6 expression is transiently induced,resulted in decreased TPL acetylation and repressor activity,thereby transcriptional activation of MYC2 target genes.Thus,the GCN5-TPL-HDA6 module main tains the homeostasis of acetylated TPL,thereby determining the transcriptional state of JA-responsive genes.Our findings uncovered a mechanism by which the TPL corepressor activity in JA signaling is activelytuned inarapidandreversiblemanner. | Chunpeng An Lei Deng Huawei Zhai Yanrong You Fangming Wu Qingzhe Zhai Alain Goossens Chuanyou Li | 2022 | Molecular Plant2022，15，8： | 2 |
| 6 | Effect of molecular weight of dexlran on the phase behavior and microstructure of preheated soy protekq/dextran mixtures显示文摘 | LI Xianghong DENG Fangming HUA Yufei | 2008 | Carbohyda-ate Polymers2008，72，1： | 1 |
| 7 | Phase behavior and microstructure of preheated soy proteins and K-carrageenan mixtures显示文摘 | LI Xianghong DENG Fangming HUA Yufei | 2008 | Food Hydrocolloids2008，22，5： | 1 |
| 8 | Mediator complex subunit MED25 physically interacts with DST to regulate spikelet number in rice显示文摘Grain number is a flexible trait and contributes significantly to grain yield.In rice,the zinc finger transcription factor DROUGHT AND SALT TOLERANCE(DST)controls grain number by directly regulating cytokinin oxidase!dehydrogenase 2(OsCKX2)expression.Although specific upstream regulators of the DST-OsCKX2 module have been identified,the mechanism employed by DST to regulate the expression of OsCKX2 remains unclear.Here,we demonstrate that DST-interacting protein 1(DIP1),known as Mediator subunit OsMED25,acts as an interacting coactivator of DST.Phenotypic analyses revealed that OsMED25-RNAi and the osmed25 mutant plants exhibited enlarged panicles,with enhanced branching and spikelet number,similar to the dst mutant.Genetic analysis indicated that OsMED25 acts in the same pathway as the DST-OsCKX2 module to regulate spikelet number per panicle.Further biochemical analysis showed that OsMED25 physically interacts with DST at the promoter region of OsCKX2,and then recruits RNA polymerase II(Pol II)to activate OsCKX2 transcription.Thus,OsMED25 was involved in the communication between DST and Pol II general transcriptional machinery to regulate spikelet number.In general,our findings reveal a novel function of OsMED25 in DST-OsCKX2 modulated transcriptional regulation,thus enhancing our un derstanding of the regulatory mechanism underlying DST-OsCKX2-mediated spikelet number. | Lihao Lin Minmin Du Shuyu Li Chuanlong Sun Fangming Wu Lei Deng Qian Chen Chuanyou Li | 2022 | Journal of Integrative Plant Biology2022，64，4： | 1 |
| 9 | Harnessing renewable energy in cloud datacenters: opportunities and challenges 显示文摘 | Deng Wei Liu Fangming | 2014 | IEEE Journals and Magazines2014，28，1： | 1 |
| 10 | Harnessing renewable energyin cloud datacenters: opportunities and challenges 显示文摘 | Deng Wei Liu Fangming Jin Hai | 2014 | IEEE Net-work2014，，： | 1 |
| 11 | Problems in Food Safety of Hunan Province and Countermeasures显示文摘In recent years,serious food safety accidents are of frequent occurrence. Although government has taken many practical and feasible measures to contain food safety accidents,new food safety accidents still emerge in large numbers. In this situation,food safety control is a long-term and arduous task to be performed jointly by many government departments. Finally,it presents corresponding countermeasures and recommendations on the basis of current situations of food safety in Hunan Province,problem causes,in combination with control measures related to food safety both at home and abroad. | Fanfan OUYANG Fangming DENG | 2014 | Asian Agricultural Research2014，6，3： | 0 |
| 12 | Online Monitoring Method for Insulator Self-explosion Based on Edge Computing and Deep Learning显示文摘Aiming at the problems of traditional centralized cloud computing which occupies large computing resources and creates high latency,this paper proposes a fault detection scheme for insulator self-explosion based on edge computing and DL(deep learning).In order to solve the high amount of computation brought by the deep neural network and meet the limited computing resources at the edge,a lightweight SSD(Single Shot MultiBox Detector)target recognition network is designed at the edge,which adopts the MobileNets network to replace VGG16 network in the original model to reduce redundant computing.In the cloud,three detection algorithms(Faster-RCNN,Retinanet,YOLOv3)with obvious differences in detection performance are selected to obtain the coordinates and confidence of the insulator self-explosion area,and then the self-explosion fault detection of the overhead transmission line is realized by a novel multimodel fusion algorithm.The experimental results show that the proposed scheme can effectively reduce the amount of uploaded data,and the average recognition accuracy of the cloud is 95.75%.In addition,it only increases the power consumption of edge devices by about 25.6W/h in their working state.Compared with the existing online monitoring technology of insulator selfexplosion at home and abroad,the proposed scheme has the advantages of low transmission delay,low communication cost and high diagnostic accuracy,which provides a new idea for online monitoring research of power internet of things equipment. | Baoquan Wei Zhongxin Xie Yande Liu Kaiyun Wen Fangming Deng Pei Zhang | 2022 | CSEE Journal of Power and Energy Systems2022，8，6： | 0 |
| 13 | A novel insulator defect detection scheme based on Deep Convolutional Auto-Encoder for small negative samples显示文摘This paper presents a novel insulator defect detection scheme based on Deep Convolutional Auto-Encoder(DCAE)for small negative samples.The proposed DCAE scheme combines the advantages of supervised learning and unsupervised learning.In order to reduce the high cost of training Deep Neural Networks,this paper pre-trained the Convolutional Neural Networks(CNN)through open labelled datasets.Through transferring learning,the encoder part of the traditional Convolutional Auto-Encoder was replaced by the first three layers of the CNN,and a small number of defect samples were used to fine-tune the parameters.A threshold discrimination scheme was designed to evaluate the model detection,realising the self-explosion detection of insulator by judging the residual result and abnormal score.The experimental results show that compared with the existing insulator self-explosion detection schemes,the proposed scheme can reduce the model training time by up to 40%,and the recognition accuracy can reach 97%.Moreover,this model does not need a large number of insulator labelled data and is especially suitable for small negative sample application. | Fangming Deng Wei Luo Baoquan Wei Yong Zuo Han Zeng Yigang He | 2022 | High Voltage2022，7，5： | 0 |
| 14 | Overexpressed PKM2 promotes macrophage phagocytosis and atherosclerosis显示文摘Background:The expression of pyruvate kinase muscle 2(PKM2)is augmented in macrophages of patients with atherosclerotic coronary artery disease.The role of PKM2 in atherosclerosis is to be determined.Methods:Global and myeloid cell-specific PKM2 knock-in mice with ApoE^(-/-)background(ApoE^(-/-),PKM2^(KI/KI)and Lyz2-cre,ApoE^(-/-),and PKM2^(flox/flox))were produced to evaluate the clinical significance of PKM2 in atherosclerosis development.Wild-type and PKM2 knock-in macrophages were isolated to assess the function of PKM2 in macrophage phagocytosis.Atherosclerotic mice were treated with PKM2 inhibitor shikonin(SKN)to evaluate the therapeutic potential of PKM2 suppression in atherosclerosis.Results:Oxidized low-density lipoprotein(oxLDL)upregulated PKM2 in macrophages.PKM2 in return promoted the uptake of oxLDL by macrophages.Overexpressed PKM2 accelerated atherosclerosis in mice.SKN blocked the progress of mouse atherosclerosis.Conclusions:PKM2 accelerates macrophage phagocytosis and atherosclerosis.Targeting PKM2 is a potential therapy for atherosclerosis. | Xiaochen Gai Fangming Liu Yuting Wu Baohui Zhang Bufu Tang Kezhuo Shang Lianmei Wang Haihong Zhang Yixin Chen Shuhui Yang Weiwei Deng Peng Li Jing Wang Hongbing Zhang | 2023 | Animal Models and Experimental Medicine2023，6，2： | 0 |
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