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| 1 | Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro显示文摘Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine.Wharton’s jelly-derived mesenchymal stem cells(WJCs)from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications.This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro.Rod-like nano-hydroxyapatite(RN-HA)and flake-like micro-hydroxyapatite(FM-HA)coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition.WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion,morphology,proliferation,and osteoblastic differentiation.The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group,demonstrating better biocompatibility.Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering. | GUAN FangXia MA ShanShan SHI XinYi MA Xun CHI LianKai LIANG Shuo CUI YuanBo WANG ZhiBin YAO Ning GUAN ShaoKang YANG Bo | 2014 | Science China(Life Sciences)2014，57，2： | 4 |
| 2 | Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor显示文摘<正>BACKGROUND:Cultures from multiple portions of umbilical cord blood mesenchymal stem cells have been shown to undergo more rapid proliferation and attachment than single portions. OBJECTIVE:To observe growth of basic fibroblast growth factor(bFGF)-induced cultures of human amnion-derived mesenchymal stem cells(AMSCs) and differentiation into neuronal-like cells. DESIGN,TIME AND SETTING:Comparative observation.The study was performed at the Laboratory of Microbiology and Immunology,Basic Medical School of Zhengzhou University from January to May 2008. METHODS:Amnia from full-term,uterine-incision delivery were donated by 12 healthy women. AMSCs were obtained by cell separation and culture techniques,and were passaged and induced by bFGF.From the third passage,a total of 1 mLAMSCs,at a density of 1.0×10~4/mL,was separately harvested from six samples,which served as group A.A total of 1 mLAMSCs,at a density of 1.0×10~4/mL,was harvested separately from the remaining six samples,which served as group B.A total of 0.5 mL from the six samples of group A and 0.5 mL from the six samples of group B were combined to form group C. MAIN OUTCOME MEASURES:Differences in cell quantity among the three groups were compared by cell quantification and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) analysis.Expression of a glial cell marker,neuron-specific enolase,and nestin was detected in the three groups by immunocytochemistry. RESULTS:Cell quantification and MTT analysis of live cells,as well as AMSC absorbance,were significantly greater in group C compared with groups A and B at 18 days of culture(P<0.05),and no significant difference was observed between groups A and B.Glial fibrillary acidic protein, neuron-specific enolase,and nestin were expressed in all groups following bFGF induction. CONCLUSION:Mixed AMSC cultures promoted proliferation,and bFGF-induced AMSCs differentiated into neuronal-like cells. | Hongliang Jiao Fangxia Guan Xiang Hu Jianbin Li Hong Shan Wei Li Jun Li Ying Du Bo Yang Yunfan Zhou | 2009 | Neural Regeneration Research2009，4，9： | 3 |
| 3 | Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles:the effect on neuron-like differentiation in vitro 显示文摘 | Guangwei Zeng Gongping Wang Fangxia Guan | 2011 | Mol Cell Biochem2011，357，12： | 1 |
| 4 | REGγ drives Lgr5^(+) stem cells to potentiate radiation induced intestinal regeneration显示文摘Leucine-rich repeat containing G protein-coupled receptor 5(Lgr5), a marker of intestinal stem cells(ISCs), is considered to play key roles in tissue homoeostasis and regeneration after acute radiation injury. However, the activation of Lgr5 by integrated signaling pathways upon radiation remains poorly understood. Here, we show that irradiation of mice with whole-body depletion or conditional ablation of REGγ in Lgr5^(+) stem cell impairs proliferation of intestinal crypts, delaying regeneration of intestine epithelial cells. Mechanistically, REGγ enhances transcriptional activation of Lgr5 via the potentiation of both Wnt and Hippo signal pathways. TEAD4 alone or cooperates with TCF4, a transcription factor mediating Wnt signaling, to enhance the expression of Lgr5. Silencing TEAD4 drastically attenuated β-catenin/TCF4 dependent expression of Lgr5. Together, our study reveals how REGγ controls Lgr5 expression and expansion of Lgr5+stem cells in the regeneration of intestinal epithelial cells.Thus, REGγ proteasome appears to be a potential therapeutic target for radiation-induced gastrointestinal disorders. | Xiangzhan Zhu Minglei Yang Zaijun Lin Solomon Kibreab Mael Ya Li Lili Zhang Yaqi Kong Yaodong Zhang Yuping Ren Jianhui Li Zimeng Wang Ying Zhang Bo yang Tingmei Huang Fangxia Guan Zhenlong Li Robb E Moses Lei Li Bing Wang Xiaotao Li Bianhong Zhang | 2022 | Science China(Life Sciences)2022，65，8： | 0 |
| 5 | Lactate metabolism in neurodegenerative diseases显示文摘Lactate,a byproduct of glycolysis,was thought to be a metabolic waste until the discovery of the Warburg effect.Lactate not only functions as a metabolic substrate to provide energy but can also function as a signaling molecule to modulate cellular functions under pathophysiological conditions.The Astrocyte-Neuron Lactate Shuttle has cla rified that lactate plays a pivotal role in the central nervous system.Moreover,protein lactylation highlights the novel role of lactate in regulating transcription,cellular functions,and disease development.This review summarizes the recent advances in lactate metabolism and its role in neurodegenerative diseases,thus providing optimal pers pectives for future research. | Chaoguang Yang Rui-Yuan Pan Fangxia Guan Zengqiang Yuan | 2024 | Neural Regeneration Research2024，19，1： | 0 |
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