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| 1 | Computational Prediction and Identification of Epstein-Barr Virus Latent Membrane Protein 2A Antigen-Specific CD8^+ T-Cell Epitopes显示文摘Epstein-Barr virus (EBV) associated nasopharyngeal carcinoma (NPC) is a high incidence tumor in Southeast Asia. Among EBV encoded proteins, latent membrane protein 2A (LMP2A) is an important antigen for T cell therapy of EBV. In this study, we predicted six HLA-A2 restricted CTL candidate epitopes of LMP2A by SYFPEITHI, NetMHC and MHCPred methods combined with the polynomial method. Subsequently, biological functions of these peptides were tested by experiments in vitro. In ELISPOT assay, the positive response of the LMP2A specific CTL stimulated by three (LMP2A264-272, LMP2A426-434 and LMP2A356-364) of six peptides respectively showed that the numbers of spots forming cells (SFC) ranged from 55.7 to 80.6 SFC/5 × 104 CD8+ T cells and the responding index (RI) ranged from 5.4 to 7. These three epitope-specific CTLs could effectively kill specific HLA-A2-expressing target cells. As a result, LMP2A264-272 (QLSPLLGAV), LMP2A426-434 (CLGGLLTMV) and LMP2A356-364 (FLYALALLL) were identified as LMP2A-specific CD8+ T-cell epitopes. It would be useful to clarify immune response toward EBV and to develop a vaccine against EBV-correlative NPC. | Bing Wang Kun Yao Genyan Liu Fangyi Xie Feng Zhou Yun Chen | 2009 | Cellular & Molecular Immunology2009，6，2： | 11 |
| 2 | Amorphous Zr(OH)4 coated LiNi0.915Co0.075Al0.01O2 cathode material with enhanced electrochemical performance for lithium ion batteries显示文摘LiNi_(0.915)Co_(0.075)Al_(0.01)O_2(NCA) with Zr(OH)_4 coating is demonstrated as high performance cathode material for lithium ion batteries(LIBs). The coated materials are synthesized via a simple dry coating method of NCA with Zr(OH)_4 powders, and then characterized with scanning electron microscopy(SEM), transmission electron microscopy(TEM) and X-ray photoelectron spectroscopy(XPS). Experimental results show that amorphous Zr(OH)_4 powders have been successfully coated on the surface of spherical NCA particles, exhibiting improved electrochemical performance. 0.50 wt% Zr(OH)_4 coated NCA delivers a capacity of 197.6 mAh/g at the first cycle and 154.3 mAh/g after 100 cycles with a capacity retention of 78.1% at 1 C rate. In comparison, the pure NCA shows a capacity of 194.6 mAh/g at the first cycle and 142.5 mAh/g after 100 cycles with a capacity retention of 73.2% at 1 C rate. Electrochemical impedance spectroscopy(EIS) results show that the coated material exhibits a lower resistance, indicating that the coating layer can efficiently suppress transition metals dissolution and decrease the side reactions at the surface between the electrode and electrolyte. Therefore, surface coating with amorphous Zr(OH)_4 is a simple and useful method to enhance the electrochemical performance of NCA-based materials for the cathode of LIBs. | Zhen Zhang Pengfei Zhou Huanju Meng Chengcheng Chen Fangyi Cheng Zhanliang Tao Jun Chen | 2017 | Journal of Energy Chemistry2017，26，3： | 5 |
| 3 | Cholecystectomy is associated with higher risk of recurrence after microwave ablation of hepatocellular carcinoma:a propensity score matching analysis显示文摘Objective:To explore the association between cholecystectomy and the prognostic outcomes of patients with hepatocellular carcinoma(H CC)who underwent microwave ablation(MWA).Methods:Patients with HCC(«=921)who underwent MWA were included and divided into cholecystectomy(n=114)and non-cholecystectomy groups(n=807).After propensity score matching(PSM)at a 1:2 ratio,overall survival(OS)and disease-free survival(DFS)rates were analyzed to compare prognostic outcomes between the cholecystectomy(«=114)and non-cholecystectomy groups(n=228).Univariate and multivariate Cox analyses were performed to assess potential risk factors for OS and DFS.Major complications were also compared between the groups.Results:After matching,no significant differences between groups were observed in baseline characteristics.The 1-,3-,and 5-year OS rates were 96.5%,82.1%,and 67.1%in the cholecystectomy group,and 97.4%,85.2%,and 74.4%in the non-cholecystectomy group(P=0.396);the 1-,3-,and 5-year DFS rates were 58.4%,34.5%,and 26.6%in the cholecystectomy group,and 73.6%,44.7%,and 32.2%in the non-cholecystectomy group(P=0.026),respectively.The intrahepatic distant recurrence rate in the cholecystectomy group was significantly higher than that in the non-cholecystectomy group(P=0.026),and the local tumor recurrence and extrahepatic recurrence rates did not significantly differ between the groups(P=0.609 and P=0.879).Multivariate analysis revealed that cholecystectomy(HR=1.364,95%Cl 1.023-1.819,P=0.035),number of tumors(2 vs.1:HR=2.744,95%Cl 1.925-3.912,P<0.001;3 vs.1:HR=3.411,95%Cl 2.021-5.759,P<0.001),and y-GT levels(HR=1.003,95%Cl 1.000-1.006,P<0.024)were independent risk factors for DFS.The best y-GT level cut-off value for predicting median DFS was 39.6 U/L(area under the curve=0.600,P<0.05).A positive correlation was observed between cholecystectomy and y-GT level(r=0.108,95%Cl-0.001-0.214,P=0.047).Subgroup analysis showed that the DFS rates were significantly higher in the non-cholecystectomy group than the cholecystectomy group when Y-GT>39.6 U/L(i3=0.044).The 5-,10-,15-,20-,and 25-year recurrence rates from the time of cholecystectomy were 2.63%,21.93%,42.11%,58.77%,and 65.79%,respectively.A significant positive correlation was observed between cholecystectomy and the time from cholecystectomy to recurrence(r=0.205,95%Cl 0.016-0.379,P=0.029).There were no significant differences in complications between groups(P=0.685).Conclusions:Patients with HCC who underwent cholecystectomy were more likely to develop intrahepatic distant recurrence after MWA,an outcome probably associated with increased y-GT levels.Moreover,the recurrence rates increased with time. | Hongcai Yang Yi Yang Jianping Dou Rui Cui Zhigang Cheng Zhiyu Han Fangyi Liu Xiaoling Yu Xiang Zhou Jie Yu Ping Liang | 2020 | Cancer Biology & Medicine2020，17，2： | 3 |
| 4 | Assessment of effective conservation of the Sichuan takin by giant panda reserves through functional zoning显示文摘The flagship species conservation strategy is widely practiced in nature reserves.However,few studies have quantitatively explored whether the current functional zoning adequately conserves the habitats of other species.To examine this problem,we selected the Tangjiahe,Xuebaoding,and Wanglang national nature reserves in southwest China and tested the protective efficiency of the giant panda(Ailuropoda melanoleuca)reserves on a local herbivore—Sichuan takin(Budorcas taxicolor tibetana)in functional zoning.We predicted that the habitat distribution of the Sichuan takin with Maxent and habitat suitability would be classified as very low,low,moderately,or highly suitable.We calculated the percentage of each type of habitat class across the core,buffer,and experimental zones in these reserves and introduced an economic index present value index to further assess the efficacy of the functional zoning in conserving the habitat of Sichuan takin.Our findings indicate that suitable habitat distributions of Sichuan takin are multicore and influenced by diverse variables in different nature reserves.The percentage of moderately suitable and highly suitable habitat in the core zone is 69%and 54%in Tangjiahe,76%and 75%in Wanglang,and 47%and 28%in Xuebaoding,respectively.The present value index of moderately and highly suitable habitat is 1.01 and 0.79(Tangjiahe),0.87 and 0.86(Wanglang),0.76 and 0.44(Xuebaoding),respectively.The efficiency of functional zoning on Sichuan takin seems lower than imagined in giant panda reserves,and species that have important impacts on local ecosystem need further estimation and greater focus. | Haiyang GAO Tianpei GUAN Di ZHU Wenwen LI Fangyi ZHOU Ding ZHAO Changlin LI Li ZHANG | 2020 | Integrative Zoology2020，15，6： | 2 |
| 5 | Percutaneous microwave ablation for liver cancer adjacent to the diaphragm显示文摘 | Meng Li Xiaoling Yu Ping Liang Fangyi Liu Baowei Dong Pei Zhou | 2012 | International Journal of Hyperthermia2012，，3： | 1 |
| 6 | Simulation study on tapered and spherical shaped charge under underwater explosion 显示文摘 | Zhou Fangyi Jiang Tao Wang Weili | 2012 | Applied Mechanics and Materials2012，，： | 1 |
| 7 | A rapid life cycle assessment method based on green features in supporting con- ceptual design显示文摘 | MENG Qiang LI Fangyi ZHOU Lirong | 2015 | International Journal of Precision Engi- neering and Manufacturing-Green Technology2015，2，2： | 1 |
| 8 | Bimetallic selenide heterostructure with directional built-in electricfield confined in N-doped carbon nanofibers for superior sodium storage with ultralong lifespan显示文摘Constructing heterostructure is considered as an effective strategy to address the sluggish electronic and ionic kinetics of anode materials for sodium ion batteries(SIBs).However,realizing the orientated growth and uniform distribution of the heterostructure is still a great challenge.Herein,the regulated novel CoSe_(2)/NiSe_(2)heterostructure confined in N-doped carbon nanofibers(CoSe_(2)/NiSe_(2)@N-C)are prepared by using Co/Ni-ZIF template,in which,the CoSe_(2)/NiSe_(2)heterostructures realize uniform distribution on a micro level.Benefiting from the unique heterostructure and N-doped carbon nanofibers,the CoSe_(2)/NiSe_(2)@N-C deliveries superior rate capability and durable cycle lifespan with a reversible capacity of 400.5 mA h g^(-1)after 5000 cycles at 2 A g^(-1).The Na-ion full battery with CoSe_(2)/NiSe_(2)@N-C anode and layered oxide cathode displays a remarkable energy density of 563 W h kg^(-1)with 241.1 W kg^(-1)at 0.1 A g^(-1).The theoretical calculations disclose that the periodic and directional built-in electric-field along with the heterointerfaces of CoSe_(2)/NiSe_(2)@N-C can accelerate electrochemical reaction kinetics.The in(ex)situ experimental measurements reveal the reversible conversion reaction and stable structure of CoSe_(2)/NiSe_(2)@N-C during Na+insertion/extraction.The study highlights the potential ability of precisely controlled heterostructure to stimulate the electrochemical performances of advanced anode for SIBs. | Junying Weng Degui Zou Wenyong Yuan Pengfei Zhou Minghui Ding Jin Zhou Hailin Cong Fangyi Cheng | 2024 | Journal of Energy Chemistry2024，91，4： | 0 |
| 9 | Amplification of unknown RNAs and RNA mixtures based on unique restriction enzyme cleavage in vitro显示文摘小 RNA,通常在底层表示了,是困难的从有限材料到达可用的层次。在这研究,我们开发了一个新奇方法放大目标 RNA。扩大过程被顺序的 RT-PCR 执行,有效分离,限制 cDNA 海滨的酶的劈开,和在从它的 cDNA 的目标 RNA 的 vitro 的加赛抄写。进 cDNA 海滨的一个唯一的茎环连接器的介绍是形成不在目标 RNA 的 cDNA 顺序的一个唯一的限制酶识别序列的关键步。这个方法能被用来从各种各样的起源放大 RNA 样品并且在放大未知小 RNA 和小 RNA 混合物有许多优点。放大 RNA 有原来的 RNA 的完整的顺序除了一额外 5 G 并且一另外 3 A 或 C。方法为 microRNA 的扩大工作了很好,交往的 piwi RNA 和二小 RNA 混合物。 | Fangyi Yang Jie Wang Yajun Ji Hao Cheng Junting Wan Zhiyu Xiao Guochun Zhou | 2010 | Acta Biochimica et Biophysica Sinica2010，42，12： | 0 |
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