|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells显示文摘体的房间能由定义因素是进导致的 pluripotent 干细胞(iPSCs ) 的 reprogrammed。reprogramming 的低效率和 oncogenes 和病毒的向量的 genomic 集成限制了 iPSCs 的潜在的申请。这里,我们报导那锂(李) ,药过去常对待心情混乱,极大地从老鼠提高 iPSC 产生胚胎的成纤维细胞和人的脐的静脉 endothelial 房间。李与(Oct4 ) 或二个因素(OS 或好) 便于 iPSC 产生。支持 reprogramming 上的李的效果仅仅部分取决于它的主要目标 GSK3β。不同于另外的 GSK3β禁止者,李不仅增加 Nanog 的表示,而且提高 Nanog 的 transcriptional 活动。我们也发现李由经由 LSD1 的 downregulation 支持 epigenetic 修正施加它的效果, H3K4 特定的 histone demethylase。将 LSD1 击倒部分在提高 reprogramming 模仿李的效果。我们的结果不仅提供一个直接方法改进 iPSC 产生效率,而且为体的房间 reprogramming 作为一个批评调节的人识别了 histone demethylase。 | Quan Wang Xinxiu Xu Jun Li Jing Liu Haifeng Gu Ru Zhang Jiekai Chen Yin Kuang Jian Fei Cong Jiang Ping Wang Duanqing Pei Sheng Ding Xin Xie | 2011 | Cell Research2011，21，10： | 23 |
| 2 | Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection显示文摘Background The influences of genomic background are confirmed in more diseases. Immunologic tolerance after intrauterine infection of hepatitis B virus is considered to occur in T cells. Cytokines work effectively in eliminating virus by immune system after hepatitis B virus infection. To explore the relationship between cytokines (tumor necrosis factor-α, interferon-γ, interleukin-4 and interleukin-10), which expressed abnormal quantity in the peripheral blood to intrauterine hepatitis B virus infectious children, gene single nucleotide polymorphism (SNP) and susceptibility to hepatitis B virus intrauterine infection. Methods This is a cross sectional study of molecular clinical epidemiology. The subjects in this study were selected from outpatients of hepatitis B vaccine follow-up special clinics of our hospital in the period. According to intrant criteria, the high risk children of hepatitis B virus (HBV) intrauterine infection were divided into immune failure group (group Ⅰ); and immune effective group (group Ⅱ) and non high risk children belonged to the control group. Four gene SNP sites of TNF-α -238, IFN-γ +874, IL-4 -590 and IL-10 -1082 were determined by real-time quantitative fluorescent polymerase chain reaction (PCR). Results The significant differences of TNF-α -238 A allele frequency were found between group Ⅰ and groupⅡ (χ2=6.797,P<0.05) and between groupⅠ and the control group (χ2=9.513,P<0.05). No evident differences of TNF-α -238 A were found between group Ⅱ and control group (χ2=0.047,P>0.05); the significant differences of IFN-γ +874 A allele frequency were found between groupⅠ and groupⅡ(χ2=7.238,P<0.05), and between groupⅠ and the control group (χ2=5.199,P<0.05). No evident differences were found between groupⅡ and the control group (χ2=0.602,P>0.05); the significant differences of IL-4 -590 C/T allele frequency were not found between groupⅠand group Ⅱ(χ2=0.632,P>0.05), also groupⅠ and the control group (χ2=0.584,P>0.05), and the groupⅡ and the control group (χ2=0.004,P>0.05)respectively; The significant differences of IL-10 -1082 G allele frequency were found between groupⅡ and groupⅠ (χ2=10.359,P<0.001), and between groupⅡ and the controls (χ2=35.418,P<0.001), but the significant differences were not found between groupⅠand the control group (χ2=1.759,P>0.05). Conclusions This study suggested the possibility that the TNF-α -238 A allele and IFN-γ +874 A allele were associated with HBV intrauterine infection. There was no evident relationship between IL-4 -590 C/T allele SNP and susceptibility to HBV intrauterine infection, but the IL-10 -1082 G allele was associated with preventive efficacy to HBV intrauterine infection. | ZHU Qi-rong GE Yan-ling GU Shao-qing YU Hui WANG Jian-she GU Xin-huan FEI Lin-e DONG Zuo-quan | 2005 | Chinese Medical Journal2005，，19： | 21 |
| 3 | Resistin induces insulin resistance, but does not affect glucose output in rat-derived hepatocytes显示文摘目的: 现在的学习的目的是观察效果抵抗在在在导出老鼠的 hepatocytes.Methods 的胰岛素敏感度和葡萄糖输出上: 老鼠肝细胞瘤房间线 H4IIE 与有教养、刺激抵抗在; supernant 葡萄糖和肝糖内容被检测。胰岛素受体底层(红外)-1 和 IRS-2,蛋白质激酶 B/Akt,肝糖 synthase kinase-3 (3 (GSK-3p ) , suppress 或表明 3 的 cytokine (SOCS-3 ) ,蛋白质内容,以及磷酸化地位被西方的弄污估计。对 SOCS-3 指导的特定的反感觉 oligodeoxynucleotides 习惯于击倒的 SOCS-3.Results :抵抗素导致了抗胰岛素性,但是在老鼠肝细胞瘤房间的affect 葡萄糖输出没衬里 H4IIE.Resistin 胰岛素的稀释多重效果包括刺激胰岛素的肝糖合成和红外的磷酸化,蛋白质激酶 B/Akt ,象 GSK-3beta.Resistin 一样,处理显著地导致了基因和 SOCS-3 的蛋白质表示,胰岛素发信号的一个已知的禁止者。而且,对 SOCS-3 处理指导的特定的反感觉 oligodeoxynucleotide 阻止了抵抗在从反对胰岛素 action.Conclusion : 主要功能抵抗在在肝上是导致胰岛素 resistance.SOCS-3 感应的可以贡献胰岛素在 H4HE hepatocytes 发信号的调停 resistin 的抑制。 | Feng LIU Tao YANG Bin WANG Min ZHANG Nan GU Jie QIU Hong-qi FAN Chun-mei ZHANG Li FEI Xiao- qing PAN Mei GUO Rong-hua CHEN Xi-rong GUO | 2008 | Acta Pharmacologica Sinica2008，29，1： | 21 |
| 4 | Expression changes and bioinformatic analysis of Wallerian degeneration after sciatic nerve injury in rat显示文摘Wallerian degeneration (WD) remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentially-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration. | Dengbing Yao Meiyuan Li Dingding Shen Fei Ding Shibi Lu Qing Zhao Xiaosong Gu | 2013 | Neuroscience Bulletin2013，29，3： | 18 |
| 5 | Prolonged exposure to resistin inhibits glucose uptake in rat skeletal muscles显示文摘瞄准:在 basaland 上估计 resistin 的行动的效果和机制在老鼠的刺激胰岛素的葡萄糖举起骨胳的肌肉 cells.Methods:老鼠 myoblasts ( L6 ) werecultured 并且区分了进刺激与单个商业 resistin 跟随的 myotubes ( 130 ng/mL , 0-24 h )或从有 resistin-expressingvectors 的 293-T 房间 transfected 的有教养的上层清液( 130 ng/mL , 0-24 h ) .Liquid 焕发数被用来测定[ 3H ]胰岛素敏感的葡萄糖 transporters GLUT4 andGLUT1 的 2-deoxyglucoseuptake .The translocation , 联系synaptosomal 的蛋白质 23 ( SNAP23 )并且充斥蛋白质内容,以及 tyrosinephosphorylation statu 有单个 resistin 或有教养的 supernatantcontaining recombinant resistin 的 L6 myotubes 的处理减少了基础、刺激胰岛素的 2-deoxyglucose 举起 andimpaired 刺激胰岛素的 GLUT4 translocation.While SNAP23 蛋白质内容被减少, noeffects 在 GLUT4 或没有影响它的蛋白质内容,也减少的 insulin-stimulatedIRS-1 酷氨酸 phosphorylation 铺平的 GLUT1 蛋白质内容 Resistin 被注意从有 resistin-expr 的 293-T 房间 transfected 的效果 ofrecombinant resistin Resistin 调整了 IRS-1 功能并且减少响应 SNAP23 mayhave 的 insulin.The downregulated 表示的 GLUT4translocation 和葡萄糖举起部分由 resistin 处理 Theseobservations 热点归因于葡萄糖举起的减少在到肥胖的类型 2 diabetesrelated 的 pathophysiology 的 resistin 的潜在的角色。 | Hong-qi FAN Nan GU Feng LIU Li FEI Xiao-qin PAN Mei GUO Rong-hua CHEN Xi-rong GUO | 2007 | Acta Pharmacologica Sinica2007，28，3： | 16 |
| 6 | Combined treatment of hepatocellular carcinoma with partial splenic embolization and transcatheter hepatic arterial chemoembolization显示文摘AIM: To prospectively evaluate the effi cacy and safety of partial splenic embolization (PSE) combined with transcatheter hepatic arterial chemoembolization (TACE) in treatment of hepatocellular carcinoma (HCC). METHODS: Fifty patients suffering from primary HCC associated with hypersplenism caused by cirrhosis were randomly assigned to 2 groups: group A receiving PSE combined with TACE (n = 26) and group B receiving TACE alone (n = 24). Follow-up examinations included calculation of peripheral blood cells (leukcytes, platelets and red blood cells) and treatment-associated complications. RESULTS: Prior to treatment, there was no signifi cant difference in sex, age, Child-Pugh grade, tumor diameter, mass pathology type and peripheral blood cell counts between the 2 groups. After treatment, leukocyte and platelet counts were significantly higher in group A during the 3-mo follow-up period (P < 0.05), but lower in group B (P < 0.05). Severe complications occurred in 3 patients (11.5%) of group A and in 19 patients (79.2%) of group B (P < 0.05), and there was no significant difference in symptoms of post-embolization syndrome, including abdominal pain, fever, mild nausea and vomiting between the 2 groups (P > 0.05). CONCLUSION: PSE combined with TACE is more effective and safe than TACE alone for patients with HCC associated with hypersplenism caused by cirrhosis. | Jin-Hua Huang Fei Gao Yang-Kui Gu Wen-Quan Li Lian-Wei Lu | 2007 | World Journal of Gastroenterology2007，13，48： | 16 |
| 7 | Liver Enzymes Concentrations Are Closely Related to Pre-diabetes:Findings of the Shanghai Diabetes Study Ⅱ (SHDS Ⅱ)显示文摘Objective To investigate the relationship of liver enzymes with hyperglycemia in a large population in Shanghai and identify the association between liver enzymes and insulin resistance.Methods A total of 3 756 participants were enrolled.Each participant underwent an oral glucose tolerance test and completed a questionnaire.Anthropometric indices were recorded and serum samples were collected for measurement.Results Liver enzymes concentrations were independently associated with i-IGT,IFG+IGT,and diabetes.With the increase of ALT and GGT concentrations,ORs for i-IGT,IFG+IGT,and diabetes increased gradually.By comparing patients in the highest quartile of GGT concentrations or ALT concentrations with those in the lowest quartile (Q1),ORs for i-IGT,IFG+IGT,or diabetes was significant after adjustment.Both ALT and GGT concentrations were linearly correlated with HOMA-IR and independently associated with HOMA-IR [ALT OR (95% CI):2.56 (1.51-4.34) P=0.00;GGT OR (95% CI):2.66 (1.53-4.65) P=0.00].Conclusion Serum ALT and GGT concentrations were closely related to pre-diabetes and diabetes in the Shanghai population and positively associated with insulin resistance. | GAO Fei PAN Jie Min HOU Xu Hong FANG Qi Chen LU Hui Juan TANG Jun Ling GU Hui Lin PAN Zhi Jian YAO You Hua SHEN Wei Zhen JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 15 |
| 8 | Relationship between IFN-γ gene polymorphism and susceptibility to intrauterine HBV infection显示文摘瞄准:为了由学习在 IFN-gamma 基因多型性,包括的 IFN-gamma+874A/T 单个核苷酸多型性(SNP ) 和 CA 之间的关系探索孩子的危险性到子宫内的 HBV 感染,重复微卫星多型性和子宫内的 HBV 感染。方法:在 IFN-gamma+874A/T 单个核苷酸多型性的 TaqMan 荧光聚合酶链反应在子宫内的 HBV 感染组被测试(组我) 并且孩子们组织的正常免疫者(组 II ) 。毛状的电气泳动在上述二个组被执行到试金 IFN-gamma CA 重复微卫星多型性。结果:AA 的频率,在并且 TT 遗传型在感染组织的子宫内的 HBV 是 67.4% , 19.6% 和 13.0% ,并且 45.2% , 30.1% 和 24.7% 分别地在正常有免疫力的孩子组织。有效差量在在二个组之间的 IFN-gamma+874 遗传型的频率分发被发现(chi2 = 5.102, P = 0.02389 ) 。在子宫内的 HBV 感染组, AA 遗传型比在正常免疫者组织是更普通的。IFN-gamma+874A 等位基因的频率在子宫内的 HBV 感染组是 77.17% ,并且 60.27% 在正常有免疫力的孩子组织。在子宫内的 HBV 感染组, IFN-gamma+874A 等位基因比在正常免疫者组织是更普通的。有效差量在在二个组之间的频率分发被发现(chi2 = 7.238, P = 0.02389,或 = 2.228, 95% CI = 1.244-3.992 ) 。(CA12 ) IFN-gamma CA 微卫星多型性的 +/(CA12 )+ 在子宫内的 HBV 感染组是 11.90% , 26.47% 在正常有免疫力的孩子组织。有效差量在在二个组之间的频率分发被发现(chi2 = 5.64, P = 0.0176 ) 。IFN-gamma CA 重复的频率在子宫内的 HBV 感染组是 25% , 43.38% 在正常有免疫力的孩子组织。IFN-gamma CA 重复的频率比在正常免疫者组织是在子宫内的 HBV 感染组的更少。有效差量在在二个组之间的频率分发被发现(chi2 = 7.548, P = 0.0060 ) 。结论:在 IFN-gamma+874A/T SNP 和子宫内的 HBV 感染之间以及在 IFN-gamma CA 微卫星多型性和子宫内的 HBV 感染之间有一种关系。IFN-gamma 基因多型性可能在决定个人的危险性到子宫内的 HBV 感染是重要的。 | Hui Yu Qi-Rong Zhu Shao-Qing Gu Lin-E Fei | 2006 | World Journal of Gastroenterology2006，12，18： | 14 |
| 9 | Parthenogenetic haploid embryonic stem cells produce fertile mice显示文摘 | Haifeng Wan Zhengquan He Mingzhu Dong Tiantian Gu Guan-Zheng Luo Fei Teng Baolong Xia Wei Li Chunjing Feng Xin Li Tianda Li Ling Shua Rui Fu Liu Wang Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Research2013，23，11： | 13 |
| 10 | Non-coding RNAs as Emerging Regulators of Neural Injury Responses and Regeneration显示文摘Non-coding RNAs(ncRNAs) are a large cluster of RNAs that do not encode proteins,but have multiple functions in diverse cellular processes.Mounting evidence indicates the involvement of nc RNAs in the physiology and pathophysiology of the central and peripheral nervous systems.It has been shown that numerous ncRNAs,especially micro RNAs and long non-coding RNAs,are differentially expressed after insults such as acquired brain injury,spinal cord injury,and peripheral nerve injury.These ncRNAs affect neuronal survival,neurite regrowth,and glial phenotype primarily by targeting specific mRNAs,resulting in translation repression or degradation of the mRNAs.An increasing number of studies have investigated the regulatory roles of micro RNAs and long non-coding RNAs in neural injury and regeneration,and thus a new research field is emerging.In this review,we highlight current progress in the field in an attempt to provide further insight into post-transcriptional changes occurring after neural injury,and to facilitate the potential use of ncRNAs for improving neural regeneration.We also suggest potential directions for future studies. | Songlin Zhou Fei Ding Xiaosong Gu | 2016 | Neuroscience Bulletin2016，32，3： | 12 |
| 11 | Effects of Nitrogen Application Time on Caryopsis Development and Grain Quality of Rice Variety Yangdao 6显示文摘A pot experiment was conducted to study the effects of different nitrogen application time (during the tillering or the booting stages) with the same nitrogen rates on the caryopsis development and grain quality of rice variety Yangdao 6. The increased nitrogen fertilizer (urea), especially applied during the booting stage, could evidently increase the milled rice rate, head rice rate and protein content in rice grains compared with the control (no nitrogen application), and decrease chalky grain rate and amylose content. Moreover, the increased nitrogen fertilizer significantly affected the caryopsis development and enhanced the grain weight when nitrogen applied during the tillering and the booting stages, especially during the booting stage. During caryopsis development the increased nitrogen fertilizer applied during the tillering and booting stages could obviously decrease the total starch and amylose contents, but not obviously for the amylopectin content in rice grain. Increased topdressing of nitrogen fertilizer, especially applied during the booting stage, had significant effect on the development and structures of amyloplasts and proteinoplasts. That is, it could change the distribution, number and shape of amyloplasts and proteinoplasts in the endosperm cells especially in grain abdomen. Compared with the control the arrangements of amyloplasts and proteinoplasts were closer, with more numbers, higher density and less interspaces each ohter. Furthermore, most amyloplasts showed polyhedron under the increased nitrogen fertilizer level. | XIONG Fei WANG Zhong Gu Yun-jie CHEN Gang ZHOU Peng | 2008 | Rice science2008，15，1： | 11 |
| 12 | Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances显示文摘Lactic acid bacteria(LAB)are a representative probiotic.As the dominant flora in the human intestinal tract,LAB can regulate the balance of human intestinal flora and improve host health.The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population,with excellent probiotics and high antibacterial activity.After 16S ribosomal RNA(rRNA)homology and phylogenetic tree analysis,potential probiotics were tested for their antibacterial activity,resistance to artificial gastrointestinal fluid and drugs,surface hydrophobicity,and safety.Three strains of LAB with acid resistance,bile salt resistance,epithelial cell adhesion,and no multidrug resistance were selected:Lactobacillus salivarius,Leuconostoc lactis,and Lactobacillus paracasei.Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L.lactis were organic acids,whereas those of L.salivarius and L.paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity.These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens. | Zhijing LIU Cong XU Ran TIAN Wan WANG Jiage MA Liya GU Fei LIU Zhanmei JIANG Juncai HOU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，7： | 10 |
| 13 | Progress in modifications and applications of fluorescent dye probe显示文摘This review summarizes the labeling technology and applications of fluorescent dye probe in biology,especially the characteristics,modification and applications of cyanine dyes.Based on the currently available modification methods of fluorescent dye probe,we discuss the studies of enhancing the water-solubility,improving the degree of biocompatibility and target-labeling,increasing the sensitivity and decreasing the toxicity of fluorescent dye.We also give a brief introduction on the modification method,that the fluorescent dye is directly introduced onto the cell surfaces by amine derivatives or azides to intensify the transferring information of aberrant cells.We suggest that fluorescent dye modified with chitosan oligosaccharide can obviously increase the degree of biocompatibility and target-labeling,and decrease the degree of toxicity. | Xuening Fei,Yingchun Gu Department of Material Science and Engineering,Tianjin Institute of Urban Construction,Tianjin 300384,China | 2009 | Progress in Natural Science:Materials International2009，19，1： | 9 |
| 14 | Transarterial embolization ablation of hepatocellular carcinoma with a lipiodol-ethanol mixture显示文摘AIM: To determine the safety and effectiveness of transarterial embolization ablation (TEA) of hepatocellular carcinoma (HCC) with a lipiodol-ethanol mixture. METHODS: Between January 1 and December 31, 2009, 15 patients with HCC (13 men/two women, aged 38-75 years) accepted TEA treatment and were enrolled in this study, including five newly diagnosed patients and 10 with refractory disease. Two months after TEA, angiography and contrast computed tomography (CT) were performed, and responses were assessed using a modified version of Response Evaluation Criteria in Solid Tumors (RECIST version 1.1). The follow-up period was to June 30, 2010. RESULTS: Every new case was treated once. Angiography was performed immediately after TEA, and showed that the tumor-feeding vessels were completely embolized and that lipiodol was densely deposited inside tumors. Two months after treatment, contrast CT showed no enhanced lesions. Alpha fetoprotein levels returned to normal in four patients and markedly decreased in another. mean ± SD survival after treatment was 10.8 ± 4.5 mo. All five patients survived during the follow-up period. Ten patients with refractory disease were treated a total of 14 times. Angiography immediately after TEA showed that blood flow to the tumors was obviously decreased in all cases, and contrast CT showed obvious depositions of lipiodol. Two months after treatment, the tumors had shrunk (6/10) or were stable (3/10). One had progressed after 2 mo and died of tumor rupture 3 mo after TEA. mean ± SD survival after treatment was 8.6 ± 4.3 mo; two patients survived during the follow-up period. Adverse effects included reversible hepatic decompensation, upper abdominal pain, and fever. CONCLUSION: TEA is an effective therapy for patients with HCC and might be more effective than transcather arterial chemoembolization for treating refractory disease. | Yang-Kui Gu, Rong-Guang Luo, Jin-Hua Huang, Qi-Jiao Si Tu, Xiao-Xia Li, Fei Gao,State Key Laboratory of Oncology in Southern China,Department of Medical Imaging and Interventional Radiology,Cancer Center,Sun Yat-sen University,Guangzhou510060,Guangdong Province,China Department of Medical Imaging and Interventional Radiology, the First Affiliated Hospital of Nan-Chang University, 17 Yongwaizheng Street, Nanchang 330006, Jiangxi Province, China | 2010 | World Journal of Gastroenterology2010，16，45： | 9 |
| 15 | Rebalancing of the gut flora and microbial metabolism is responsible for the anti-arthritis effect of kaempferol显示文摘Kaempferol is a natural flavonol that possesses various pharmacological activities,including anti-arthritis effects,yet the underlying mechanisms remain controversial.To evaluate the anti-arthritis efficacy and the underlying mechanisms of kaempferol,collagen-induced arthritis(CIA)mice were treated with kaempferol intragastrically(200 mg·kg^−1·d^−1)and intraperitoneally(20 mg·kg^−1·d^−1).Pharmacodynamic and pharmacokinetic studies showed that the oral administration of kaempferol produced distinct anti-arthritis effects in model mice with arthritis in terms of the spleen index,arthritis index,paw thickness,and inflammatory factors;the bioavailability(1.5%,relative to that of the intraperitoneal injection)and circulatory exposure of kaempferol(Cmax=0.23±0.06 ng/mL)and its primary metabolite kaempferol-3-O-glucuronide(Cmax=233.29±89.64 ng/mL)were rather low.In contrast,the intraperitoneal injection of kaempferol caused marginal anti-arthritis effects,although it achieved a much higher in vivo exposure.The much higher kaempferol content in the gut implicated a potential mechanism involved in the gut.Analysis of 16S ribosomal RNA revealed that CIA caused imbalance of 14 types of bacteria at the family level,whereas kaempferol largely rebalanced the intestinal microbiota in CIA mice.A metabolomics study showed that kaempferol treatment significantly reversed the perturbation of metabolites involved in energy production and the tryptophan,fatty acid and secondary bile acid metabolisms in the gut contents of the CIA mice.In conclusion,we demonstrate for the first time that the high level of kaempferol in the gut regulates the intestinal flora and microbiotic metabolism,which are potentially responsible for the anti-arthritis activities of kaempferol. | Li-xiang Aa Fei Fei Qi Qi Run-bin Sun Sheng-hua Gu Zi-zhen Di Ji-ye Aa Guang-ji Wang Chang-xiao Liu | 2020 | Acta Pharmacologica Sinica2020，41，1： | 9 |
| 16 | Altered microRNA expression following sciatic nerve resection in dorsal root ganglia of rats显示文摘MicroRNAs (miRNAs ) 是否定地 post-transcriptionally 调整基因表示的小、非编码的 RNA (22 核苷酸) 的一个类,通过目标 mRNAs 的翻译抑制或降级的任何一个。我们揭开了在在在在在用 microarray 分析的老鼠的臀部的神经的切除术以后的 1, 4, 7,和 14 天的背面的根 ganglia (DRG ) 的 miRNAs 的表示的以前未知的改变。32 显著地, upregulated 和 18 downregulated miRNAs 在跟随臀部的神经损害的四个次点在 DRG 被识别。四的表示连续地 deregulated miRNAs,由即时 Taqman 聚合酶链反应分析了,与 microarray 数据一致(upregulated:miR-21, miR-221;downregulated:miR-500, miR-551b ) 。为这些 miRNAs 的潜在的目标,在臀部的神经切除术以后改变了,主要涉及细胞的进程的神经系统开发,多细胞的 organismal 开发,和规定。这研究在一只老鼠在臀部的神经的切除术以后在 DRG 表明了 miRNAs 的不同参与模型,和它可以也在说明为神经新生负责的分子的机制作出贡献。 | Bin Yu Songlin Zhou Tianmei Qian Yongjun Wang Fei Ding Xiaosong Gu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，11： | 9 |
| 17 | DIAGNOSTIC VALUE OF WHOLE BODY DIFFUSION WEIGHTED IMAGING FOR SCREENING PRIMARY TUMORS OF PATIENTS WITH METASTASES显示文摘Objective To evaluate the values of whole body diffusion weighted imaging (DWI) in screening primary unknown tumor in patients with metastases. Methods Totally, 34 patients with metastases of primary unknown tumors were scanned with whole body DWI, and conventional magnetic resonance (MR) imaging was performed if suspected lesions were detected. All the metastases including 27 cases of osseous metastases, 2 brain metastases, 2 liver metastases, 1 pulmonary multiple metastasis, 1 neck metastasis and 1 malignant ascites, were diagnosed by computed tomography, single photon emission computed tomography, or MR imaging. For the proven primary tumors diagnosed by biopsy or pathology of surgical specimens, apparent diffusion coefficient (ADC) values of the primary and metastatic lesions were measured respectively. The sensitivity and specificity of this technique for screening primary tumors were evaluated. Results We found 24 cases with suspected primary lesions, in which 23 lesions were proved to be primary tumors, and 1 was proved to be benign lesion. And no definite primary lesion was found in 10 cases on whole body DWI, but in which 1 case was diagnosed with primary tumor by biopsy later, and the other 9 cases remained unknown within follow-up of over half a year. The difference was not significant in ADC values between primary and metastatic lesions (P>0.05). The sensitivity and specificity of whole body DWI for searching primary tumors was 95.8% and 90.0%, respectively. Conclusion Combined with conventional MR scanning, whole body DWI can help to search primary lesions of patients with metastases. | Tai-fu Gu Xin-lan Xiao Fei Sun Jian-hua Yin Hai Zhao | 2008 | Chinese Medical Sciences Journal2008，23，3： | 9 |
| 18 | Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress显示文摘The Arabidopsis 25K GeneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, Xinjiang, XJqhx) genes was up- or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key tran-scription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China. | HE Fei KANG JiuQing ZHOU Xin SU Zhen QU LiJia GU HongYa | 2008 | Chinese Science Bulletin2008，53，19： | 9 |
| 19 | The Roles of acyI-CoA: Diacylglycerol Acyltransferase 2 Genes in the Biosynthesis of Triacylglycerols by the Green Algae Chlamydomonas reinhardtii显示文摘Dear Editor, Triacylglycerols(triglycerides)(TAGs),as the major storage forms of energy,mainly are stored in adipocytes,myocytes,enterocytes,hepatocytes,and mammary epithelial cells in mammals,oilseeds in plants,and lipid droplets in microorganisms(Yen et al.,2008). | Xiao-Dong Deng Bo Gu Ya-Jun Li Xin-Wen Hu Jian-Chun Guo Xiao-Wen Fei | 2012 | Molecular Plant2012，5，4： | 9 |
| 20 | Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2显示文摘Host cellular receptors play key roles in the determination of virus tropism and pathogenesis.However,little is known about SARS-CoV-2 host receptors with the exception of ACE2.Furthermore,ACE2 alone cannot explain the multi-organ tropism of SARS-CoV-2 nor the clinical differences between SARS-CoV-2 and SARS-CoV,suggesting the involvement of other receptor(s).Here,we performed genomic receptor profiling to screen 5054 human membrane proteins individually for interaction with the SARS-CoV-2 capsid spike(S)protein.Twelve proteins,including ACE2,ASGR1,and KREMEN1,were identified with diverse S-binding affinities and patterns.ASGR1 or KREMEN1 is sufficient for the entry of SARS-CoV-2 but not SARS-CoV in vitro and in vivo.SARS-CoV-2 utilizes distinct ACE2/ASGR1/KREMEN1(ASK)receptor combinations to enter different cell types,and the expression of ASK together displays a markedly stronger correlation with virus susceptibility than that of any individual receptor at both the cell and tissue levels.The cocktail of ASK-related neutralizing antibodies provides the most substantial blockage of SARS-CoV-2 infection in human lung organoids when compared to individual antibodies.Our study revealed an interacting host receptome of SARS-CoV-2,and identified ASGR1 and KREMEN1 as alternative functional receptors that play essential roles in ACE2-independent virus entry,providing insight into SARS-CoV-2 tropism and pathogenesis,as well as a community resource and potential therapeutic strategies for further COVID-19 investigations. | Yunqing Gu Jun Cao Xinyu Zhang Hai Gao Yuyan Wang Jia Wang Juan He Xiaoyi Jiang Jinlan Zhang Guanghui Shen Jie Yang Xichen Zheng Gaowei Hu Yuanfei Zhu Shujuan Du Yunkai Zhu Rong Zhang Jianqing Xu Fei Lan Di Qu Guoliang Xu Yun Zhao Dong Gao Youhua Xie Min Luo Zhigang Lu | 2022 | Cell Research2022，32，1： | 8 |
您所在的机构暂未开通此部分的功能，请联系管理员