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| 1 | Dual amine-and brnsted acid-catalyzed α-allylic alkylation of aldehydes显示文摘 | Xu Liwen Gao Guang Gu Fenglei | 2010 | Advanced Synthesis & Catalysis2010，352，9： | 1 |
| 2 | Blocking the Natural Killer (NK) Cell Inhibitory Receptor NKG2A Increases Activity of Human NK Cells and Clears HBV Infection in Mice显示文摘 | Fenglei Li Hairong Wei Haiming Wei Yufeng Gao Long Xu Wenwei Yin Rui Sun Zhigang Tian | 2012 | Gastroenterology2012，，： | 1 |
| 3 | Distribution pattern and geochemical analysis of rare earth elements in deep-ocean sediments显示文摘The content and distribution pattern of rare earth elements(REEs)in surface sediments from the Eastern and Western Pacifi c Ocean,the Northern and Southern Atlantic Ocean,and the Southwestern Indian Ocean were explored and the resources and geochemical characteristics of REEs in deep-ocean sediments from diff erent oceans were studied.The total REE abundances(ΣREE)in the diff erent oceans ranged as follows:Eastern Pacifi c,56.88–500.02μg/g;Western Pacifi c,290.68–439.94μg/g;Northern Atlantic,55.33–154.90μg/g;Southern Atlantic,40.83–69.30μg/g;and Southwestern Indian Ocean,20.24–64.76μg/g.Their corresponding LREE(La-Eu)/HREE(Gd-Lu)average values were 5.18,5.86,9.01,5.21,and 4.59,which indicated that the light REEs were all evidently enriched.δEu andδCe showed slight Eu-negative anomalies and signifi cant Ce-positive anomalies in all sediments.Although the contents of REEs in the sediments varied among the diff erent oceans,the distribution patterns of REEs were similar,and the correlation coeffi cient was greater than 0.9290.In the Eastern Pacifi c sediments,ΣREE showed a signifi cantly positive correlation with Co,Cu,Zn,Mn,Mo and a weak correlation with Fe.In the Western Pacifi c and Southern Atlantic sediments,ΣREE presented no obvious correlation and a weakly negative correlation with Co,Cu,Zn,Mn,Mo and Fe,respectively.ΣREE in the Southwestern Indian Ocean sediments positively correlated with Cu,Zn,Mn,Mo,Fe,and had a weakly negative correlation with Co. | Jingxi LI Chengjun SUN Fenghua JIANG Fenglei GAO Yifan ZHENG | 2021 | Journal of Oceanology and Limnology2021，39，1： | 1 |
| 4 | Preparation of MnO2/graphene composite as electrode material for supercapacitors显示文摘 | Qian Yong Lu Shunbao Gao Fenglei | | 0，，10： | 1 |
| 5 | Functional verification of the diphtheria toxin A gene in a recombinant system显示文摘Diphtheria toxin A (DTA), a segment of the diphtheria toxin (tox), inhibits protein synthesis in cells. When released from a cell, DTA is nontoxic and cannot enter other cells independently without the help of diphtheria toxin B. In this study, we artificially synthesized the DTA gene sequence and cloned it into pEGFP-N1 to generate the recombinant vector pEGFP-N1-DTA. This recombinant vector was then transfected into 293T cells to observe the effect of DTA protein expression on enhanced green fluorescent protein (EGFP) protein expression and the proliferation of 293T cells. After 48 h, high levels of EGFP expression were seen in control pEGFP-N1-transfected cells, whereas very low levels were seen in cells transfected with pEGFP-N1-DTA. Reverse transcription polymerase chain reaction confirmed the expression of the DTA gene in cells transfected with pEGFP-N1-DTA. Further, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed a significant difference in cell proliferation between the control group and the pEGFP-N1-DTA-transfected group. Using the expression of EGFP expression as an indicator, this study revealed that DTA expression can inhibit intracellular protein synthesis and cell proliferation. | Jingfeng Zhang Hengxi Wei Xinzheng Guo Minghua Hu Fenglei Gao Li Li Shouquan Zhang | 2013 | Journal of Animal Science and Biotechnology2013，4，1： | 0 |
| 6 | Efficient production of chimeric mice from embryonic stem cells injected into 4-to 8-cell and blastocyst embryos显示文摘Background: Production of chimeric mice is a useful tool for the elucidation of gene function. After successful isolation of embryonic stem(ES) cell lines, there are many methods for producing chimeras, including co-culture with the embryos, microinjection of the ES cells into pre-implantation embryos, and use of tetraploid embryos to generate the full ES-derived transgenic mice. Here, we aimed to generate the transgenic ES cell line, compare the production efficiency of chimeric mice and its proportion to yield the male chimeric mice by microinjected ES cells into 4-to 8-cell and blastocysts embryos with the application of Piezo-Micromanipulator(PMM), and trace the fate of the injected ES cells. Results: We successfully generated a transgenic ES cell line and proved that this cell line still maintained pluripotency. Although we achieved a satisfactory chimeric mice rate, there was no significant difference in the production of chimeric mice using the two different methods, but the proportion of the male chimeric mice in the 4-to 8-cell group was higher than in the blastocyst group. We also found that there was no tendency for ES cells to aggregate into the inner cell mass using in vitro culture of the chimeric embryos, indicating that they aggregated randomly. Conclusions: These results showed that the PMM method is a convenient way to generate chimeric mice and microinjection of ES cells into 4-to 8-cell embryos can increase the chance of yielding male chimeras compared to the blastocyst injection. These results provide useful data in transgenic research mediated by ES cells. | Minhua Hu Hengxi Wei Jingfeng Zhang Yinshan Bai Fenglei Gao Li Li Shouquan Zhang | 2013 | Journal of Animal Science and Biotechnology2013，4，4： | 0 |
| 7 | Reconfigurable robot based on modular joint concept显示文摘 | 史士财 Xie Zongwu Gao Xiaohui Jin Minghe Zhu Yingyuan Ni Fenglei Liu Hong Kraemer Erich Hirzinger Gerd | 2006 | High Technology Letters2006，12，4： | 0 |
| 8 | A Review of Microplastics in China Marine Waters显示文摘Microplastics(<5 mm)are ubiquitous in the environment and can pose potential danger to the ecosystem and even human health.As the sink of microplastics,the ocean,especially the densely populated coastal area,has become a hotspot for research on microplastic pollution.In the last decade,the research of marine microplastics has been rapidly increasing in China.This review summarized the microplastic research conducted in China marine waters so far,and introduced the trends and progress of microplastic research in the four seas along the coast of China.We reviewed and compared the current sampling,extraction,and identification methodologies of China's microplastic research.According to the sampling method,the 30 reviewed studies were separated into two categories,trawl sampling and bulk sampling,to summarize relevant data,including abundance,sizes,shapes,colors and polymer types of microplastics.The main results showed that the distribution of microplastics in China's marine environment varied significantly,with offshore mariculture zones and the South China Sea being the most contaminated areas.Transparent,granules(or pellets)and fibers were the most dominant microplastic colors and shapes,and the size of microplastics was influenced significantly by the sampling method.Polyethylene(PE),polypropylene(PP)and polystyrene(PS)were the most common polymer types found in the China Sea,accounting for 49.96%,29.97%,and 12.38%of the total studies,respectively.Compared with other global data,China's coastal microplastic pollution is at an intermediate level and does not seem to be a major microplastic pollution source. | GAO Fenglei LI Jingxi HU Jun LI Xianguo SUN Chengjun | 2023 | Journal of Ocean University of China2023，22，5： | 0 |
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