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| 1 | Genetic diversity of the wild Asian lotus (Nelumbo nucifera) from Northern China显示文摘Asian lotus(Nelumbo nucifera Gaertn.)is an aquatic plant with ornamental,cultural,economic,and ecological values.China has abundant germplasm resources of Asian lotus.However,in many areas,the wild Asian lotuses have been destroyed and the germplasms is now facing extinction.In addition,the knowledge of the genetic diversity of the wild Asian lotus in China is poor.To identify and protect the germplasms of Chinese wild Asian lotus,eleven genomic-SSR primers,three EST-SSR primers,and three chloroplast DNA primers were used to investigate the genetic diversity among 69 samples of wild Asian lotus from 25 locations in northern China.The genetic diversity of 27 samples of wild Asian lotus from southern China and other countries,the ancient Asian lotus,Asian lotus cultivars from China,and Asian-American hybrids was also compared.The genetic diversity of the wild Asian lotus from northern China was characterized as medium,and themean values of observed heterozygosity(Ho)and expected heterozygosity(He)were 0.087 and 0.552,respectively.Based on a UPGMA dendrogram and STRUCTURE analysis,the wild Asian lotus samples in northern China were divided into three groups.The wild Asian lotus samples from northern China contained 16 haplotypes.The Nei’s genetic distance between the wild Asian lotus samples from the Songhua River basin and the Liao River basin in northeastern China was relatively small,and these germplasms might be relatively primitive compared to those from other regions.This study provides essential information regarding the genetic diversity of the wild Asian lotus resources in northern China,and provides a basis for further analysis of population-level genetic evolution through high-throughput sequencing. | Yanrong Fu Fengluan Liu Shuo Li Daike Tian Li Dong Yuchu Chen Yan Su | 2021 | Horticultural Plant Journal2021，7，5： | 3 |
| 2 | Quercetin-derivatives painting the yellow petals of American lotus(Nelumbo lutea)and enzymatic basis for their accumulation显示文摘American lotus(Nelumbo lutea)is one of the two species in Nelumbo and has only yellow flower.Identification of total flavonoids showed wild American lotus contained almost only flavonols with quercetin 3-O-glucuronide to be the dominant pigment.The variation tendency of the total flavonol content was coincident with yellow color variation of petals during flower development.To understand the mechanism of accumulation and constituent of pigments in petals,three pivotal genes,NlFLS1,NlFLS2 and NlFLS3,which were predicted to encode flavonol synthases were isolated and characterized by analyses of basic bioinformatics,temporal and spatial expression patterns and enzymatic activity.Their temporal expression levels showed the same variation tendency,which was also consistent with the development-dependent variation of total flavonol content.Spatial expression patterns indicated the three genes should function in petals.All the three proteins were demonstrated to be bifunctional dioxygenase,possessing both flavonol synthase activity and flavanone 3-hydroxylase activity.Besides,other flavonol biosynthesis related genes were also investigated on their expression levels to give more clues on the mechanism.Substrate preferences of the three FLSs,substrate competitions between the FLSs and other flavonol biosynthesis related enzymes,and the greatly differential expression levels between F3’H(flavonoid 3-hydroxylase)and F3’5’H(flavonoid 3,5-hydroxylase)contributed to the flavonol constituent in the petals of America lotus,namely abundant quercetin-derivatives while very few kaempferol-derivatives and myricetin-derivatives. | Qingqing Liu Dasheng Zhang Fengluan Liu Zhuoxing Liu Xiaohan Wang Yong Yang Shanshan Li Hanchun Li Daike Tian Liangsheng Wang | 2023 | Horticultural Plant Journal2023，9，1： | 1 |
| 3 | CEREBROCARDIAC SYNDROME ON PATIENTS OF OVER SEVENTY AGE AND EEG STUDY显示文摘Objectives: To observe cerebrocardiac syndrome on patients of the over seventy age and it's change of EEG. Methods: Brain CT and ECG were examined in 50 patients of the over seventy age with cerebrocardiac syndrome. Results: There were obvious changes in the brain CT and ECG. Conclusion:There were obvious changes of brain CT and ECG in cerebrocardiac syndrome of over seventy age patients. | LIU Mingshun, MEI Fengjun, LI Fengluan, LIU Qingrui, GU Lanjie Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, 050011 | 2005 | 现代电生理学杂志2005，12，3： | 0 |
| 4 | THE CONTRAST STUDY ON LEVEL DIAGNOSIS OF CT AND BA IN CEREBROVASCULAR DISEASES显示文摘Objective:To explore level diagnosis on CT and BA in cerebrovascular diseases.Method:CT and BA were examined in 53 patients with cerebrovascular diseases and compared in level diagnosis.Result:The sides on level diagonsis of CT and BA were identical.The rang of diseases was larger in BA than that in CT.Conclusion:BA can help level diagnosis in cerebrovascular diseases.The level diagnosis of BA and CT were basically identical. | Mingshun Liu Dan He Fengluan Li Shucai Wu | 2010 | 现代电生理学杂志2010，17，1： | 0 |
| 5 | THE STUDY APPLIED ON BA IN DIAGNOSIS OF CEREBRAL ARTERIOSCLEROSIS显示文摘目的:探讨脑电地形图在脑动脉硬化诊断中的应用价值。方法:90例脑动脉硬化患者进行了脑电地形图及脑CT检查,并两者进行了对比。结果:90例脑动脉硬化患者的脑电地形图改变是明显的,与脑CT对比有显著差异,P<0.01。结论:脑电地形图在脑动脉硬化患者诊断中有重要价值。 | Mingshun Liu,Po Ma,Fengluan Li,Jing Hu,Fengiun Mei | 2010 | 现代电生理学杂志2010，17，3： | 0 |
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